DE60306771T3 - Neue peptidzusammensetzungen und deren verwendung, insbesondere zur herstellung von pharmazeutischen zusammensetzungen mit wirkung gegen das hepatitis-c-virus - Google Patents
Neue peptidzusammensetzungen und deren verwendung, insbesondere zur herstellung von pharmazeutischen zusammensetzungen mit wirkung gegen das hepatitis-c-virus Download PDFInfo
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- DE60306771T3 DE60306771T3 DE60306771T DE60306771T DE60306771T3 DE 60306771 T3 DE60306771 T3 DE 60306771T3 DE 60306771 T DE60306771 T DE 60306771T DE 60306771 T DE60306771 T DE 60306771T DE 60306771 T3 DE60306771 T3 DE 60306771T3
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
- C12N2770/00011—Details
- C12N2770/24011—Flaviviridae
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- Communicable Diseases (AREA)
- Biophysics (AREA)
- Genetics & Genomics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Oncology (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Peptides Or Proteins (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FR0206111 | 2002-05-17 | ||
| FR0206111A FR2839722A1 (fr) | 2002-05-17 | 2002-05-17 | Nouvelles compositions peptidiques et leur utilisation notamment dans la preparation de compositions pharmaceutiques actives contre le virus de l'hepatite c |
| PCT/FR2003/001478 WO2003097677A2 (fr) | 2002-05-17 | 2003-05-15 | Nouvelles compostions peptidiques et leur utilisation notamment dans la preparation de compositions pharmaceutiques actives contre le virus de l'hepatite c |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| DE60306771D1 DE60306771D1 (de) | 2006-08-24 |
| DE60306771T2 DE60306771T2 (de) | 2007-08-09 |
| DE60306771T3 true DE60306771T3 (de) | 2010-09-30 |
Family
ID=29286584
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE60306771T Expired - Lifetime DE60306771T3 (de) | 2002-05-17 | 2003-05-15 | Neue peptidzusammensetzungen und deren verwendung, insbesondere zur herstellung von pharmazeutischen zusammensetzungen mit wirkung gegen das hepatitis-c-virus |
Country Status (12)
| Country | Link |
|---|---|
| US (5) | US7393831B2 (enExample) |
| EP (1) | EP1506225B2 (enExample) |
| JP (1) | JP4309837B2 (enExample) |
| CN (1) | CN1653086B (enExample) |
| AT (1) | ATE332921T1 (enExample) |
| AU (1) | AU2003255577A1 (enExample) |
| DE (1) | DE60306771T3 (enExample) |
| DK (1) | DK1506225T4 (enExample) |
| ES (1) | ES2268411T5 (enExample) |
| FR (1) | FR2839722A1 (enExample) |
| PT (1) | PT1506225E (enExample) |
| WO (1) | WO2003097677A2 (enExample) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2839722A1 (fr) | 2002-05-17 | 2003-11-21 | Bio Merieux | Nouvelles compositions peptidiques et leur utilisation notamment dans la preparation de compositions pharmaceutiques actives contre le virus de l'hepatite c |
| FR2855758B1 (fr) * | 2003-06-05 | 2005-07-22 | Biomerieux Sa | Composition comprenant la polyproteine ns3/ns4 et le polypeptide ns5b du vhc, vecteurs d'expression incluant les sequences nucleiques correspondantes et leur utilisation en therapeutique |
| FR2862648B1 (fr) * | 2003-11-21 | 2006-02-03 | Biomerieux Sa | Nouveau peptide immunogene et nouveaux epitopes et utilisations notamment dans la preparation de compositions pharmaceutiques actives contre le virus de l 'hepatite c |
| US20080249283A1 (en) * | 2004-04-30 | 2008-10-09 | Tomoya Miyakawa | Hla-Binding Peptides, Precursors Thereof, Dna Fragments and Recombinant Vectors that Code for Those Peptide Sequences |
| MX2008011361A (es) * | 2006-03-09 | 2008-09-15 | Transgene Sa | Proteina de fusion no-estructural del virus de hepatitis c. |
| JP5031827B2 (ja) | 2006-06-01 | 2012-09-26 | 程云 | 予防或いは肝損傷を治療するペプチッド |
| EP2646459B1 (en) | 2010-12-02 | 2020-01-08 | Bionor Immuno AS | Peptide scaffold design |
Family Cites Families (46)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US132321A (en) * | 1872-10-15 | Improvement in platform-scales | ||
| US4007844A (en) * | 1976-02-02 | 1977-02-15 | Maxon Industries, Inc. | Self folding platform |
| US4087007A (en) * | 1976-12-01 | 1978-05-02 | Leyman Manufacturing Co. | Cargo platform system |
| US4198188A (en) * | 1977-04-07 | 1980-04-15 | Perkins William V | Load elevator mechanism for unstable loads |
| US4563121A (en) * | 1983-02-22 | 1986-01-07 | Leyman Manufacturing Corp. | Cargo elevator system |
| US4576541A (en) * | 1984-02-29 | 1986-03-18 | Leyman Manufacturing Corp. | Safety latch for a cargo platform |
| US4627784A (en) * | 1986-01-21 | 1986-12-09 | Venco Manufacturing, Inc. | Loading and unloading apparatus for a vehicle |
| US4725185A (en) * | 1986-05-12 | 1988-02-16 | Maxon Industries, Inc. | Fail safe brake for rail type lifts |
| US4806062A (en) * | 1987-10-29 | 1989-02-21 | Maxon Industries, Inc. | Stowable lift for freight vehicles |
| US5683864A (en) * | 1987-11-18 | 1997-11-04 | Chiron Corporation | Combinations of hepatitis C virus (HCV) antigens for use in immunoassays for anti-HCV antibodies |
| US4930969A (en) * | 1988-08-11 | 1990-06-05 | Langer Hartford P | Rail lift gate apparatus and storage scheme |
| KR0185373B1 (ko) | 1989-03-17 | 1999-05-01 | 로버트 피. 블랙버언 | Hcv 폴리단백질에서 유래되는 hcv 아미노산 서열 부분을 포함하는 폴리펩티드 및 그 사용 |
| US5106726A (en) * | 1990-02-16 | 1992-04-21 | United Biomedical, Inc. | Synthetic peptides specific for the detection of antibodies to HCV |
| US5747239A (en) * | 1990-02-16 | 1998-05-05 | United Biomedical, Inc. | Synthetic peptides specific for the detection of antibodies to HCV, diagnosis of HCV infection and preventions thereof as vaccines |
| US5639594A (en) * | 1990-02-16 | 1997-06-17 | United Biomedical, Inc. | Linear and branched peptides effective in diagnosing and detecting non-A, non-B hepatitis |
| KR940000755B1 (ko) * | 1990-02-16 | 1994-01-29 | 유나이티드 바이오메디칼 인코오포레이티드 | Hcv에 대한 항체 검출, hcv 감염의 진단 및 백신으로서의 그 예방에 특히 적합한 합성 펩티드 |
| US5582968A (en) * | 1990-02-16 | 1996-12-10 | United Biomedical, Inc. | Branched hybrid and cluster peptides effective in diagnosing and detecting non-A, non-B hepatitis |
| HU217025B (hu) * | 1990-04-04 | 1999-11-29 | Chiron Corp. | Hepatitis C vírus-(HCV) antigénkészítmények az anti-HCV-antitestek kimutatására immunoassay-kben |
| US5712087A (en) * | 1990-04-04 | 1998-01-27 | Chiron Corporation | Immunoassays for anti-HCV antibodies employing combinations of hepatitis C virus (HCV) antigens |
| US5157504A (en) | 1990-04-20 | 1992-10-20 | Fuji Photo Film Co., Ltd. | Copy cassette supplying apparatus |
| CA2047792C (en) * | 1990-07-26 | 2002-07-02 | Chang Y. Wang | Synthetic peptides specific for the detection of antibodies to hcv, diagnosis of hcv infection and prevention thereof as vaccines |
| US5122026A (en) * | 1990-09-17 | 1992-06-16 | Leyman Manufacturing Corp. | Cargo platform with plural storage positions |
| US6274148B1 (en) * | 1990-11-08 | 2001-08-14 | Chiron Corporation | Hepatitis C virus asialoglycoproteins |
| ES2188583T3 (es) * | 1991-06-24 | 2003-07-01 | Chiron Corp | Polipeptidos para el virus de la hepatitis c (hcv). |
| US5263808A (en) * | 1991-11-27 | 1993-11-23 | Leyman Manufacturing Corp. | Automatic storage latch system for a cargo platform |
| BR9406652A (pt) | 1993-03-05 | 1996-09-10 | Cytel Corp | Composição |
| US5709995A (en) * | 1994-03-17 | 1998-01-20 | The Scripps Research Institute | Hepatitis C virus-derived peptides capable of inducing cytotoxic T lymphocyte responses |
| US5513943A (en) * | 1994-05-16 | 1996-05-07 | Tatcom, Inc. | Load elevator with columnar power assemblies |
| US20030095980A1 (en) * | 1994-07-29 | 2003-05-22 | Geert Maertens | Purified hepatitis C virus envelope proteins for diagnostic and therapeutic use |
| GB2294690B (en) * | 1994-11-01 | 1998-10-28 | United Biomedical Inc | Peptides effective for diagnosis and detection of hepatitis C infection |
| US5597282A (en) * | 1995-08-29 | 1997-01-28 | Leyman Manufacturing Corporation | Method and apparatus for unstacking and unloading a stacked load from one level to another level |
| US6534482B1 (en) * | 1998-05-13 | 2003-03-18 | Epimmune, Inc. | Expression vectors for stimulating an immune response and methods of using the same |
| ES2371432T3 (es) * | 1998-05-13 | 2012-01-02 | Epimmune Inc. | Vectores de expresión para estimular una respuesta inmune y procedimientos de uso de los mismos. |
| US7108855B2 (en) * | 1998-06-24 | 2006-09-19 | Innogenetics N.V. | Purified hepatitis C virus envelope proteins for diagnostic and therapeutic use |
| AU765940B2 (en) * | 1998-06-24 | 2003-10-02 | Innogenetics N.V. | Particles of HCV envelope proteins: use for vaccination |
| WO2001021189A1 (en) * | 1999-07-19 | 2001-03-29 | Epimmune Inc. | Inducing cellular immune responses to hepatitis c virus using peptide and nucleic acid compositions |
| AU1236801A (en) * | 1999-10-27 | 2001-05-08 | Chiron Corporation | Activation of hcv-specific t cells |
| FR2809402A1 (fr) * | 2000-05-26 | 2001-11-30 | Dev Des Antigenes Combinatoire | Bibliotheques peptidiques combinatoires convergentes et leur application a la vaccination contre le virus de l'hepatite c |
| AUPQ776100A0 (en) * | 2000-05-26 | 2000-06-15 | Australian National University, The | Synthetic molecules and uses therefor |
| AU2002219711B8 (en) | 2000-12-08 | 2006-03-02 | Academisch Ziekenhuis Leiden | Long peptides of 22-45 amino acid residues that induce and/or enhance antigen specific immune responses |
| DE10131978A1 (de) * | 2001-07-02 | 2003-01-30 | Witte Velbert Gmbh & Co Kg | Drehfallenverschluss |
| FR2839722A1 (fr) | 2002-05-17 | 2003-11-21 | Bio Merieux | Nouvelles compositions peptidiques et leur utilisation notamment dans la preparation de compositions pharmaceutiques actives contre le virus de l'hepatite c |
| US7695960B2 (en) * | 2003-06-05 | 2010-04-13 | Transgene S.A. | Composition comprising the polyprotein NS3/NS4 and the polypeptide NS5B of HCV, expression vectors including the corresponding nucleic sequences and their therapeutic use |
| US7172689B2 (en) * | 2004-02-20 | 2007-02-06 | Bratten Jack R | Arrangement and method for maintaining a minimum flow velocity in the coolant return of a machine tool coolant filtration system |
| US7491026B2 (en) * | 2005-03-17 | 2009-02-17 | Saf-Holland, Inc. | Lift gate assembly |
| US7484921B2 (en) * | 2005-08-24 | 2009-02-03 | Murphy Michael O | Lift gate system |
-
2002
- 2002-05-17 FR FR0206111A patent/FR2839722A1/fr not_active Withdrawn
-
2003
- 2003-05-15 US US10/514,762 patent/US7393831B2/en not_active Expired - Fee Related
- 2003-05-15 JP JP2004506350A patent/JP4309837B2/ja not_active Expired - Fee Related
- 2003-05-15 EP EP03752815A patent/EP1506225B2/fr not_active Expired - Lifetime
- 2003-05-15 ES ES03752815T patent/ES2268411T5/es not_active Expired - Lifetime
- 2003-05-15 DK DK03752815.5T patent/DK1506225T4/da active
- 2003-05-15 WO PCT/FR2003/001478 patent/WO2003097677A2/fr not_active Ceased
- 2003-05-15 DE DE60306771T patent/DE60306771T3/de not_active Expired - Lifetime
- 2003-05-15 AT AT03752815T patent/ATE332921T1/de active
- 2003-05-15 CN CN038111349A patent/CN1653086B/zh not_active Expired - Fee Related
- 2003-05-15 PT PT03752815T patent/PT1506225E/pt unknown
- 2003-05-15 AU AU2003255577A patent/AU2003255577A1/en not_active Abandoned
-
2006
- 2006-08-11 US US11/503,245 patent/US20070072176A1/en not_active Abandoned
-
2008
- 2008-06-03 US US12/132,321 patent/US8080525B2/en not_active Expired - Fee Related
- 2008-09-16 US US12/211,385 patent/US8067228B2/en not_active Expired - Fee Related
-
2009
- 2009-12-17 US US12/653,751 patent/US8293528B2/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| DK1506225T4 (da) | 2010-07-05 |
| US20070020285A1 (en) | 2007-01-25 |
| ES2268411T5 (es) | 2010-05-28 |
| DE60306771D1 (de) | 2006-08-24 |
| PT1506225E (pt) | 2006-12-29 |
| WO2003097677A3 (fr) | 2004-03-25 |
| JP4309837B2 (ja) | 2009-08-05 |
| US8067228B2 (en) | 2011-11-29 |
| US20070072176A1 (en) | 2007-03-29 |
| AU2003255577A8 (en) | 2003-12-02 |
| DK1506225T3 (da) | 2006-11-13 |
| US20110020398A1 (en) | 2011-01-27 |
| US7393831B2 (en) | 2008-07-01 |
| WO2003097677A2 (fr) | 2003-11-27 |
| US8293528B2 (en) | 2012-10-23 |
| CN1653086A (zh) | 2005-08-10 |
| US20090087449A1 (en) | 2009-04-02 |
| US8080525B2 (en) | 2011-12-20 |
| US20090075879A1 (en) | 2009-03-19 |
| DE60306771T2 (de) | 2007-08-09 |
| FR2839722A1 (fr) | 2003-11-21 |
| ES2268411T3 (es) | 2007-03-16 |
| ATE332921T1 (de) | 2006-08-15 |
| JP2006511442A (ja) | 2006-04-06 |
| CN1653086B (zh) | 2012-05-23 |
| EP1506225B2 (fr) | 2010-03-17 |
| AU2003255577A1 (en) | 2003-12-02 |
| EP1506225B1 (fr) | 2006-07-12 |
| EP1506225A2 (fr) | 2005-02-16 |
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Legal Events
| Date | Code | Title | Description |
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| 8363 | Opposition against the patent | ||
| 8366 | Restricted maintained after opposition proceedings |