DE60301622T2 - Auf primer verlängerung basierendes verfahren, das nukleotide verwendet, die über spaltbare linker markiert sind - Google Patents

Auf primer verlängerung basierendes verfahren, das nukleotide verwendet, die über spaltbare linker markiert sind Download PDF

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Publication number
DE60301622T2
DE60301622T2 DE60301622T DE60301622T DE60301622T2 DE 60301622 T2 DE60301622 T2 DE 60301622T2 DE 60301622 T DE60301622 T DE 60301622T DE 60301622 T DE60301622 T DE 60301622T DE 60301622 T2 DE60301622 T2 DE 60301622T2
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DE
Germany
Prior art keywords
nucleotide
primer
mole
linkers
labeled derivative
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60301622T
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German (de)
English (en)
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DE60301622D1 (de
Inventor
Charlotta Olsson
Nigel Tooke
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Qiagen GmbH
Original Assignee
Biotage AB
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Filing date
Publication date
Priority claimed from SE0201024A external-priority patent/SE0201024D0/xx
Application filed by Biotage AB filed Critical Biotage AB
Publication of DE60301622D1 publication Critical patent/DE60301622D1/de
Application granted granted Critical
Publication of DE60301622T2 publication Critical patent/DE60301622T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
DE60301622T 2002-04-04 2003-04-04 Auf primer verlängerung basierendes verfahren, das nukleotide verwendet, die über spaltbare linker markiert sind Expired - Lifetime DE60301622T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US36959902P 2002-04-04 2002-04-04
SE0201024A SE0201024D0 (sv) 2002-04-04 2002-04-04 New method
PCT/SE2003/000547 WO2003085135A1 (en) 2002-04-04 2003-04-04 New method

Publications (2)

Publication Number Publication Date
DE60301622D1 DE60301622D1 (de) 2005-10-20
DE60301622T2 true DE60301622T2 (de) 2006-06-14

Family

ID=28793693

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60301622T Expired - Lifetime DE60301622T2 (de) 2002-04-04 2003-04-04 Auf primer verlängerung basierendes verfahren, das nukleotide verwendet, die über spaltbare linker markiert sind

Country Status (8)

Country Link
US (1) US20050244827A1 (https=)
EP (1) EP1495137B1 (https=)
JP (1) JP4230364B2 (https=)
AT (1) ATE304610T1 (https=)
AU (1) AU2003214765B2 (https=)
CA (1) CA2481495C (https=)
DE (1) DE60301622T2 (https=)
WO (1) WO2003085135A1 (https=)

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* Cited by examiner, † Cited by third party
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AU2001296645A1 (en) 2000-10-06 2002-04-15 The Trustees Of Columbia University In The City Of New York Massive parallel method for decoding dna and rna
US9708358B2 (en) 2000-10-06 2017-07-18 The Trustees Of Columbia University In The City Of New York Massive parallel method for decoding DNA and RNA
US7057026B2 (en) 2001-12-04 2006-06-06 Solexa Limited Labelled nucleotides
GB0129012D0 (en) 2001-12-04 2002-01-23 Solexa Ltd Labelled nucleotides
WO2004018497A2 (en) 2002-08-23 2004-03-04 Solexa Limited Modified nucleotides for polynucleotide sequencing
US11008359B2 (en) 2002-08-23 2021-05-18 Illumina Cambridge Limited Labelled nucleotides
US7414116B2 (en) 2002-08-23 2008-08-19 Illumina Cambridge Limited Labelled nucleotides
CA2513899C (en) * 2003-01-29 2013-03-26 454 Corporation Methods of amplifying and sequencing nucleic acids
WO2005060345A2 (en) * 2003-12-22 2005-07-07 Biotage Ab New method for sequencing-by-synthesis
GB0517097D0 (en) 2005-08-19 2005-09-28 Solexa Ltd Modified nucleosides and nucleotides and uses thereof
WO2008042067A2 (en) 2006-09-28 2008-04-10 Illumina, Inc. Compositions and methods for nucleotide sequencing
GB2457402B (en) 2006-12-01 2011-10-19 Univ Columbia Four-color DNA sequencing by synthesis using cleavable fluorescent nucleotide reversible terminators
DK2725107T3 (da) 2007-10-19 2019-01-02 Univ Columbia DNA-sekventering med ikke-fluorescerende nukleotidreversible terminatorer og ddNTP'er modificeret med spaltbart mærke og nukleinsyre omfattende inosin med reversible terminatorer
WO2009051807A1 (en) 2007-10-19 2009-04-23 The Trustees Of Columbia University In The City Of New York Design and synthesis of cleavable fluorescent nucleotides as reversible terminators for dna sequencing by synthesis
CN101878258B (zh) 2007-11-28 2013-10-23 巴斯夫欧洲公司 液体稳定剂混合物
WO2009100382A1 (en) * 2008-02-07 2009-08-13 Pacific Biosciences Of California, Inc. Cis reactive oxygen quenchers integrated into linkers
WO2010111674A2 (en) 2009-03-27 2010-09-30 Life Technologies Corporation Methods and apparatus for single molecule sequencing using energy transfer detection
US9146248B2 (en) 2013-03-14 2015-09-29 Intelligent Bio-Systems, Inc. Apparatus and methods for purging flow cells in nucleic acid sequencing instruments
WO2014139596A1 (en) 2013-03-15 2014-09-18 Illumina Cambridge Limited Modified nucleosides or nucleotides
US9591268B2 (en) 2013-03-15 2017-03-07 Qiagen Waltham, Inc. Flow cell alignment methods and systems
WO2014144883A1 (en) 2013-03-15 2014-09-18 The Trustees Of Columbia University In The City Of New York Raman cluster tagged molecules for biological imaging
AU2017354876A1 (en) * 2016-11-04 2019-06-20 Qiagen Sciences, Llc Thiol-containing cleave reagents and oxidative wash
AU2019212849B2 (en) * 2018-01-26 2024-02-29 F. Hoffmann-La Roche Ag Radiolabelled oligonucleotides and process for their preparation

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4910300A (en) * 1985-12-11 1990-03-20 Chiron Corporation Method for making nucleic acid probes
US5268486A (en) * 1986-04-18 1993-12-07 Carnegie-Mellon Unversity Method for labeling and detecting materials employing arylsulfonate cyanine dyes
US5783384A (en) * 1992-01-13 1998-07-21 President And Fellows Of Harvard College Selection of binding-molecules
US5516664A (en) * 1992-12-23 1996-05-14 Hyman; Edward D. Enzymatic synthesis of repeat regions of oligonucleotides
JP3761197B2 (ja) * 1995-12-27 2006-03-29 タカラバイオ株式会社 新規ｄｎａポリメラーゼ
JP2001517948A (ja) * 1997-04-01 2001-10-09 グラクソ、グループ、リミテッド 核酸配列決定法
US6485944B1 (en) * 1997-10-10 2002-11-26 President And Fellows Of Harvard College Replica amplification of nucleic acid arrays
US6280947B1 (en) * 1999-08-11 2001-08-28 Exact Sciences Corporation Methods for detecting nucleotide insertion or deletion using primer extension
ATE556149T1 (de) * 1999-02-23 2012-05-15 Caliper Life Sciences Inc Manipulation von mikropartikeln in mikrofluidischen systemen
US6818395B1 (en) * 1999-06-28 2004-11-16 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences
WO2001094546A2 (en) * 2000-06-02 2001-12-13 Dna Sciences, Inc. Primer extension using a mixture of labelled and unlabelled nucleotides

Also Published As

Publication number Publication date
ATE304610T1 (de) 2005-09-15
JP2005521422A (ja) 2005-07-21
DE60301622D1 (de) 2005-10-20
JP4230364B2 (ja) 2009-02-25
WO2003085135A1 (en) 2003-10-16
AU2003214765B2 (en) 2007-07-26
EP1495137B1 (en) 2005-09-14
US20050244827A1 (en) 2005-11-03
EP1495137A1 (en) 2005-01-12
CA2481495C (en) 2011-08-30
CA2481495A1 (en) 2003-10-16
AU2003214765A1 (en) 2003-10-20

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Owner name: QIAGEN GMBH, 40724 HILDEN, DE