CA2481495C - Primer extension based method employing nucleotides labelled via cleavable linkers - Google Patents

Primer extension based method employing nucleotides labelled via cleavable linkers Download PDF

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Publication number
CA2481495C
CA2481495C CA2481495A CA2481495A CA2481495C CA 2481495 C CA2481495 C CA 2481495C CA 2481495 A CA2481495 A CA 2481495A CA 2481495 A CA2481495 A CA 2481495A CA 2481495 C CA2481495 C CA 2481495C
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CA
Canada
Prior art keywords
nucleotide
mole
label
primer
labeled
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
CA2481495A
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English (en)
French (fr)
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CA2481495A1 (en
Inventor
Charlotta Olsson
Nigel Tooke
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Qiagen GmbH
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Qiagen GmbH
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Filing date
Publication date
Priority claimed from SE0201024A external-priority patent/SE0201024D0/xx
Application filed by Qiagen GmbH filed Critical Qiagen GmbH
Publication of CA2481495A1 publication Critical patent/CA2481495A1/en
Application granted granted Critical
Publication of CA2481495C publication Critical patent/CA2481495C/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
CA2481495A 2002-04-04 2003-04-04 Primer extension based method employing nucleotides labelled via cleavable linkers Expired - Lifetime CA2481495C (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US36959902P 2002-04-04 2002-04-04
SE0201024-7 2002-04-04
US60/369,599 2002-04-04
SE0201024A SE0201024D0 (sv) 2002-04-04 2002-04-04 New method
PCT/SE2003/000547 WO2003085135A1 (en) 2002-04-04 2003-04-04 New method

Publications (2)

Publication Number Publication Date
CA2481495A1 CA2481495A1 (en) 2003-10-16
CA2481495C true CA2481495C (en) 2011-08-30

Family

ID=28793693

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2481495A Expired - Lifetime CA2481495C (en) 2002-04-04 2003-04-04 Primer extension based method employing nucleotides labelled via cleavable linkers

Country Status (8)

Country Link
US (1) US20050244827A1 (https=)
EP (1) EP1495137B1 (https=)
JP (1) JP4230364B2 (https=)
AT (1) ATE304610T1 (https=)
AU (1) AU2003214765B2 (https=)
CA (1) CA2481495C (https=)
DE (1) DE60301622T2 (https=)
WO (1) WO2003085135A1 (https=)

Families Citing this family (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9708358B2 (en) 2000-10-06 2017-07-18 The Trustees Of Columbia University In The City Of New York Massive parallel method for decoding DNA and RNA
JP2004510433A (ja) 2000-10-06 2004-04-08 ザ・トラスティーズ・オブ・コランビア・ユニバーシティー・イン・ザ・シティー・オブ・ニューヨーク Ｄｎａおよびｒｎａを解読するための大量並行方法
US7057026B2 (en) 2001-12-04 2006-06-06 Solexa Limited Labelled nucleotides
GB0129012D0 (en) 2001-12-04 2002-01-23 Solexa Ltd Labelled nucleotides
US11008359B2 (en) 2002-08-23 2021-05-18 Illumina Cambridge Limited Labelled nucleotides
US7414116B2 (en) 2002-08-23 2008-08-19 Illumina Cambridge Limited Labelled nucleotides
EP3002289B1 (en) 2002-08-23 2018-02-28 Illumina Cambridge Limited Modified nucleotides for polynucleotide sequencing
WO2005003375A2 (en) * 2003-01-29 2005-01-13 454 Corporation Methods of amplifying and sequencing nucleic acids
WO2005060345A2 (en) * 2003-12-22 2005-07-07 Biotage Ab New method for sequencing-by-synthesis
GB0517097D0 (en) 2005-08-19 2005-09-28 Solexa Ltd Modified nucleosides and nucleotides and uses thereof
US8399188B2 (en) 2006-09-28 2013-03-19 Illumina, Inc. Compositions and methods for nucleotide sequencing
WO2008069973A2 (en) 2006-12-01 2008-06-12 The Trustees Of Columbia University In The City Of New York Four-color dna sequencing by synthesis using cleavable fluorescent nucleotide reversible terminators
DK2725107T3 (da) 2007-10-19 2019-01-02 Univ Columbia DNA-sekventering med ikke-fluorescerende nukleotidreversible terminatorer og ddNTP'er modificeret med spaltbart mærke og nukleinsyre omfattende inosin med reversible terminatorer
WO2009051807A1 (en) 2007-10-19 2009-04-23 The Trustees Of Columbia University In The City Of New York Design and synthesis of cleavable fluorescent nucleotides as reversible terminators for dna sequencing by synthesis
JP5431352B2 (ja) 2007-11-28 2014-03-05 ビーエーエスエフ ソシエタス・ヨーロピア 液体安定剤混合物
AU2009212165A1 (en) 2008-02-07 2009-08-13 Pacific Biosciences Of California, Inc. CIS reactive oxygen quenchers integrated into linkers
US20100261185A1 (en) 2009-03-27 2010-10-14 Life Technologies Corporation Labeled enzyme compositions, methods and systems
US9146248B2 (en) 2013-03-14 2015-09-29 Intelligent Bio-Systems, Inc. Apparatus and methods for purging flow cells in nucleic acid sequencing instruments
WO2014144883A1 (en) 2013-03-15 2014-09-18 The Trustees Of Columbia University In The City Of New York Raman cluster tagged molecules for biological imaging
US9591268B2 (en) 2013-03-15 2017-03-07 Qiagen Waltham, Inc. Flow cell alignment methods and systems
BR112015022448B1 (pt) 2013-03-15 2020-12-08 Illumina Cambridge Limited molécula de nucleotídeo ou nucleosídeo modificado, métodos para preparar o crescimento de polinucleotídeo complementar a polinucleotídeo alvo de fita simples em reação de sequenciação e para determinar a sequência de polinucleotídeo alvo de fita simples e kit
CN110506121A (zh) * 2016-11-04 2019-11-26 奇根科学有限责任公司 含硫羟基的切割试剂和氧化洗涤剂
EP3743116B1 (en) * 2018-01-26 2024-07-10 F. Hoffmann-La Roche AG Radiolabelled oligonucleotides and process for their preparation

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4910300A (en) * 1985-12-11 1990-03-20 Chiron Corporation Method for making nucleic acid probes
US5268486A (en) * 1986-04-18 1993-12-07 Carnegie-Mellon Unversity Method for labeling and detecting materials employing arylsulfonate cyanine dyes
US5783384A (en) * 1992-01-13 1998-07-21 President And Fellows Of Harvard College Selection of binding-molecules
US5516664A (en) * 1992-12-23 1996-05-14 Hyman; Edward D. Enzymatic synthesis of repeat regions of oligonucleotides
CN1552869A (zh) * 1995-12-27 2004-12-08 宝酒造株式会社 新型dna聚合酶
US20020055100A1 (en) * 1997-04-01 2002-05-09 Kawashima Eric H. Method of nucleic acid sequencing
US6485944B1 (en) * 1997-10-10 2002-11-26 President And Fellows Of Harvard College Replica amplification of nucleic acid arrays
US6280947B1 (en) * 1999-08-11 2001-08-28 Exact Sciences Corporation Methods for detecting nucleotide insertion or deletion using primer extension
DE60045917D1 (de) * 1999-02-23 2011-06-16 Caliper Life Sciences Inc Sequenzierung durch inkorporation
US6818395B1 (en) * 1999-06-28 2004-11-16 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences
WO2001094546A2 (en) * 2000-06-02 2001-12-13 Dna Sciences, Inc. Primer extension using a mixture of labelled and unlabelled nucleotides

Also Published As

Publication number Publication date
AU2003214765B2 (en) 2007-07-26
EP1495137B1 (en) 2005-09-14
AU2003214765A1 (en) 2003-10-20
ATE304610T1 (de) 2005-09-15
DE60301622T2 (de) 2006-06-14
EP1495137A1 (en) 2005-01-12
DE60301622D1 (de) 2005-10-20
CA2481495A1 (en) 2003-10-16
WO2003085135A1 (en) 2003-10-16
JP4230364B2 (ja) 2009-02-25
US20050244827A1 (en) 2005-11-03
JP2005521422A (ja) 2005-07-21

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