DE60217393D1 - Verfahren zur kombinierten parallelen zuführung von agentien und elektroporation für zellstrukturen und verwendung davon - Google Patents

Verfahren zur kombinierten parallelen zuführung von agentien und elektroporation für zellstrukturen und verwendung davon

Info

Publication number
DE60217393D1
DE60217393D1 DE60217393T DE60217393T DE60217393D1 DE 60217393 D1 DE60217393 D1 DE 60217393D1 DE 60217393 T DE60217393 T DE 60217393T DE 60217393 T DE60217393 T DE 60217393T DE 60217393 D1 DE60217393 D1 DE 60217393D1
Authority
DE
Germany
Prior art keywords
cell structure
agents
tubes
electroporation
cell structures
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60217393T
Other languages
English (en)
Other versions
DE60217393T2 (de
Inventor
Owe Orwar
Mattias Karlsson
Kerstin Nolkrantz
Cecilia Farre
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Cellectricon AB
Original Assignee
Cellectricon AB
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cellectricon AB filed Critical Cellectricon AB
Publication of DE60217393D1 publication Critical patent/DE60217393D1/de
Application granted granted Critical
Publication of DE60217393T2 publication Critical patent/DE60217393T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/87Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61NELECTROTHERAPY; MAGNETOTHERAPY; RADIATION THERAPY; ULTRASOUND THERAPY
    • A61N1/00Electrotherapy; Circuits therefor
    • A61N1/18Applying electric currents by contact electrodes
    • A61N1/20Applying electric currents by contact electrodes continuous direct currents
    • A61N1/30Apparatus for iontophoresis, i.e. transfer of media in ionic state by an electromotoric force into the body, or cataphoresis
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G01N2035/1027General features of the devices
    • G01N2035/1034Transferring microquantities of liquid
    • G01N2035/1039Micropipettes, e.g. microcapillary tubes
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G01N35/1065Multiple transfer devices
    • G01N35/1074Multiple transfer devices arranged in a two-dimensional array

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Molecular Biology (AREA)
  • Radiology & Medical Imaging (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Crystals, And After-Treatments Of Crystals (AREA)
DE60217393T 2001-11-27 2002-11-27 Verfahren zur kombinierten parallelen zuführung von agentien und elektroporation für zellstrukturen und verwendung davon Expired - Lifetime DE60217393T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
SE0103957 2001-11-27
SE0103957 2001-11-27
PCT/SE2002/002192 WO2003046170A1 (en) 2001-11-27 2002-11-27 A method for combined parallel agent delivery and electroporation for cell structures and use thereof

Publications (2)

Publication Number Publication Date
DE60217393D1 true DE60217393D1 (de) 2007-02-15
DE60217393T2 DE60217393T2 (de) 2007-10-18

Family

ID=20286108

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60217393T Expired - Lifetime DE60217393T2 (de) 2001-11-27 2002-11-27 Verfahren zur kombinierten parallelen zuführung von agentien und elektroporation für zellstrukturen und verwendung davon

Country Status (8)

Country Link
US (4) US20050019921A1 (de)
EP (2) EP1448771B1 (de)
JP (2) JP2005510237A (de)
AT (1) ATE350471T1 (de)
AU (2) AU2002353741A1 (de)
CA (2) CA2468511A1 (de)
DE (1) DE60217393T2 (de)
WO (2) WO2003046170A1 (de)

Families Citing this family (38)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7456012B2 (en) 1997-11-06 2008-11-25 Cellectricon Ab Method and apparatus for spatially confined electroporation
DE60217393T2 (de) 2001-11-27 2007-10-18 Cellectricon Ab Verfahren zur kombinierten parallelen zuführung von agentien und elektroporation für zellstrukturen und verwendung davon
EP2368635A3 (de) * 2002-02-12 2012-03-07 Cellectricon Ab Systeme und Verfahren zur schnellen Änderung der Lösungsumgebung um Sensoren
AU2003278461A1 (en) 2002-10-16 2004-05-04 Cellectricon Ab Nanoelectrodes and nanotips for recording transmembrane currents in a plurality of cells
US8058056B2 (en) * 2004-03-12 2011-11-15 The Regents Of The University Of California Method and apparatus for integrated cell handling and measurements
US8293524B2 (en) 2006-03-31 2012-10-23 Fluxion Biosciences Inc. Methods and apparatus for the manipulation of particle suspensions and testing thereof
WO2009029519A2 (en) * 2007-08-24 2009-03-05 David Mark Evans Multiplexed electroporation apparatus
WO2009067142A1 (en) * 2007-11-21 2009-05-28 The Regents Of The University Of California Controlled multi-holes electroporation
EP2772532B1 (de) 2008-03-12 2023-06-21 Cellectricon Ab Vorrichtung und Verfahren zur Spitzenausrichtung auf Multiwellplatten
ITCO20080016A1 (it) * 2008-04-22 2009-10-23 Giuseppe Caccia Manipolo per elettroporazione di prodotti farmacologici e/o naturali e/o estetici su strati cutanei a spessore contenuto
DE102008045830A1 (de) 2008-09-05 2010-03-11 Cinogy Gmbh Verfahren zur Behandlung eines lebende Zellen enthaltenden biologischen Materials
WO2010064991A1 (en) * 2008-12-01 2010-06-10 Nanyang Polytechnic Electroporation apparatus and method
BR112012011435B1 (pt) 2009-11-13 2020-06-23 Intuitive Surgical Operations, Inc. Mecanismo de instrumento cirúrgico, conjunto robótico de instrumento cirúrgico e sistema robótico de instrumento cirúrgico
EP2651505A4 (de) 2010-12-15 2014-06-11 Old Dominion Univ Res Found Elektroporationsinduzierte elektrosensibilisierung
AU2012255988B2 (en) * 2011-05-13 2017-07-13 The Regents Of The University Of California Photothermal substrates for selective transfection of cells
WO2013006343A1 (en) * 2011-07-01 2013-01-10 Beckman Coulter, Inc. Low carryover liquid handling probe for an automated analyzer
JP6035948B2 (ja) * 2011-08-04 2016-11-30 日本精工株式会社 圧電アクチュエータ及びマニピュレータ
CN102335479B (zh) * 2011-09-28 2013-03-13 上海交通大学 植入式微型电渗流可控输药芯片及其制备方法
CN102872531B (zh) * 2011-09-28 2014-11-19 上海交通大学 一种电渗流可控输药芯片电渗驱动单元的制备方法
LT2768942T (lt) 2011-10-17 2020-04-10 Massachusetts Institute Of Technology Pristatymas į ląstelės vidų
CA2905999A1 (en) 2013-03-15 2014-09-25 The Regents Of The University Of California High-throughput cargo delivery into live cells using photothermal platforms
US20160108432A1 (en) * 2013-05-16 2016-04-21 Advanced Liquid Logic, Inc. Droplet actuator for electroporation and transforming cells
KR102304167B1 (ko) 2013-08-16 2021-09-24 메사추세츠 인스티튜트 오브 테크놀로지 세포로의 선택적 물질 전달
AU2015235932B2 (en) 2014-03-28 2021-08-05 The Regents Of The University Of California Efficient delivery of large cargos into cells on a porous substrate
WO2016011059A1 (en) * 2014-07-14 2016-01-21 President And Fellows Of Harvard College Drug cocktail analyses using microscale vortex-assisted electroporation
US20170183644A1 (en) * 2014-07-14 2017-06-29 President And Fellows Of Harvard College Drug cocktail analyses using microscale vortex assisted electroporation
PL226157B1 (pl) 2014-10-21 2017-06-30 Inst Tech Materiałów Elektronicznych Mikrosonda do elektroporacji selektywnej i sposob wytwarzania takiej mikrosondy
RU2020139190A (ru) 2014-10-31 2021-01-26 Массачусетс Инститьют Оф Текнолоджи Доставка биомолекул в клетки иммунной системы
JP6846345B2 (ja) 2014-11-14 2021-03-24 マサチューセッツ インスティテュート オブ テクノロジー 破壊及び場による細胞への化合物及び組成物の送達
EP3037518B1 (de) * 2014-12-28 2020-05-06 Femtobiomed Inc. Vorrichtung zum einbringen von material in eine zelle
KR102428463B1 (ko) * 2014-12-28 2022-08-03 주식회사 펨토바이오메드 세포에 물질을 주입하는 장치 및 제조 방법
EP3037517A1 (de) * 2014-12-28 2016-06-29 Femtofab Co., Ltd. Verfahren zur modifizierung einer zelle durch einführung eines materials in die zelle
EP3245294A4 (de) 2015-01-12 2018-05-30 Massachusetts Institute of Technology Geneditierung durch mikrofluidische freisetzung
CN104536186B (zh) * 2015-01-13 2017-12-08 合肥鑫晟光电科技有限公司 一种识别装置和对位系统
US10167502B2 (en) 2015-04-03 2019-01-01 Fluxion Biosciences, Inc. Molecular characterization of single cells and cell populations for non-invasive diagnostics
US10987670B2 (en) 2015-04-14 2021-04-27 President And Fellows Of Harvard College Electrode array for vortex-assisted electroporation
JP6925984B2 (ja) 2015-07-09 2021-08-25 マサチューセッツ インスティテュート オブ テクノロジー 無核細胞への物質の送達
US11884960B2 (en) * 2019-08-16 2024-01-30 Terragene Llc Biological indicator for determining the efficacy of a steam or heat sterilization process and its method of use

Family Cites Families (67)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US584268A (en) * 1897-06-08 John edward mellor
US52A (en) * 1836-10-11 Drawing
US3867201A (en) 1971-08-11 1975-02-18 Mallory & Co Inc P R Hermetically sealed electrolyte battery and method of filling without breaking hermetic seal
DE3737607A1 (de) * 1987-11-05 1989-05-24 Hoefelmayr Bio Melktech Verfahren und vorrichtung zur durchfuehrung von messungen an einer schaeumenden fluessigkeit
US5389069A (en) 1988-01-21 1995-02-14 Massachusetts Institute Of Technology Method and apparatus for in vivo electroporation of remote cells and tissue
EP0398960B1 (de) 1988-01-21 1995-12-06 Massachusetts Institute Of Technology Molekültransport durch gewebe mit der verwendung von elektroporation
WO1990004645A1 (en) 1988-10-21 1990-05-03 Molecular Devices Corporation Methods and apparatus for detecting the effect of cell affecting agents on living cells
US5135478A (en) * 1989-05-10 1992-08-04 Drug Delivery Systems Inc. Multi-signal electrical transdermal drug applicator
US5137817A (en) 1990-10-05 1992-08-11 Amoco Corporation Apparatus and method for electroporation
US5208145A (en) 1991-05-06 1993-05-04 The Salk Institute For Biological Studies Assay for the presence, in cultured cells, of functional ligand-gated channels, and substances which enhance intracellular lithium accumulation by cultured cells via such channels
US5449492A (en) 1992-01-15 1995-09-12 List Electronics Device for determining concentration-dependent electrophysiological parameters in a series of measurements
US5501662A (en) 1992-05-22 1996-03-26 Genetronics, Inc. Implantable electroporation method and apparatus for drug and gene delivery
US5597699A (en) 1992-09-30 1997-01-28 Lanzara; Richard G. Method for determining drug compositions to prevent desensitization of cellular receptors
WO1994020841A1 (en) 1993-03-05 1994-09-15 University Of Wollongong Pulsed electrochemical detection using polymer electroactive electrodes
US5993434A (en) 1993-04-01 1999-11-30 Genetronics, Inc. Method of treatment using electroporation mediated delivery of drugs and genes
US5702359A (en) 1995-06-06 1997-12-30 Genetronics, Inc. Needle electrodes for mediated delivery of drugs and genes
DE69409626T2 (de) * 1994-01-28 1998-11-05 Hewlett Packard Gmbh Kapillar aus Kunststoff für Kapillarelektroforese und Verfahren zu dessen Herstellung
IL108775A (en) 1994-02-25 2003-09-17 Univ Ramot Method for efficient incorporation of molecules into cells
CA2194010A1 (en) * 1994-06-24 1996-01-04 Ooi Wong Pulsatile delivery systems of biologically active agents using electro voltage pulsing for controlling membrane permeability
WO1996010170A1 (en) 1994-09-29 1996-04-04 The Board Of Trustees Of The Leland Stanford Junior University Capillary-based separation methods for identifying bioactive analytes in a mixture
DE19529371C3 (de) 1995-08-10 2003-05-28 Nmi Univ Tuebingen Mikroelektroden-Anordnung
US6283951B1 (en) * 1996-10-11 2001-09-04 Transvascular, Inc. Systems and methods for delivering drugs to selected locations within the body
US6762036B2 (en) 1995-11-08 2004-07-13 Trustees Of Boston University Cellular physiology workstations for automated data acquisition and perfusion control
US5874268A (en) 1996-09-23 1999-02-23 Duke University Method of introducing exogenous compounds into cells by electroporation and apparatus for same
US6352535B1 (en) 1997-09-25 2002-03-05 Nanoptics, Inc. Method and a device for electro microsurgery in a physiological liquid environment
US5885430A (en) * 1996-10-04 1999-03-23 Spectrumedix Corporation Capillary tube holder for an electrophoretic apparatus
EP0938674B1 (de) 1996-11-16 2005-06-01 NMI Naturwissenschaftliches und Medizinisches Institut an der Universität Tübingen in Reutlingen Stiftung Bürgerlichen Rechts Mikroelementenanordnung, verfahren zum kontaktieren von in einer flüssigen umgebung befindlichen zellen und verfahren zum herstellen einer mikroelementenanordnung
US5873849A (en) 1997-04-24 1999-02-23 Ichor Medical Systems, Inc. Electrodes and electrode arrays for generating electroporation inducing electrical fields
WO1998050791A1 (en) 1997-05-01 1998-11-12 Neurosearch A/S An automatic electrode positioning apparatus
SE9702112D0 (sv) 1997-06-04 1997-06-04 Holdingbolaget Vid Goeteborgs Method and apparatus for detection of a receptor antagonist
US6241666B1 (en) 1997-07-03 2001-06-05 Cardiac Pathways Corp. Ablation catheter tip with a buffer layer covering the electrode
US6241701B1 (en) 1997-08-01 2001-06-05 Genetronics, Inc. Apparatus for electroporation mediated delivery of drugs and genes
US6055453A (en) * 1997-08-01 2000-04-25 Genetronics, Inc. Apparatus for addressing needle array electrodes for electroporation therapy
US7456012B2 (en) 1997-11-06 2008-11-25 Cellectricon Ab Method and apparatus for spatially confined electroporation
SE9704076D0 (sv) 1997-11-06 1997-11-06 Holdingbolaget Vid Goeteborgs Method for permeabilisation of cell structures and use thereof
US6208893B1 (en) 1998-01-27 2001-03-27 Genetronics, Inc. Electroporation apparatus with connective electrode template
WO2000034434A1 (en) * 1998-12-07 2000-06-15 Acacia Biosciences, Inc. Multi-channel electrode arrays
US6713291B2 (en) 1999-01-28 2004-03-30 Alan D. King Electrodes coated with treating agent and uses thereof
US6193647B1 (en) 1999-04-08 2001-02-27 The Board Of Trustees Of The University Of Illinois Microfluidic embryo and/or oocyte handling device and method
US6300108B1 (en) 1999-07-21 2001-10-09 The Regents Of The University Of California Controlled electroporation and mass transfer across cell membranes
SE9902817D0 (sv) 1999-07-30 1999-07-30 A & Science Invest Ab A method for selective electrofusion of at least two fusion partners having cell-like membranes
US6326177B1 (en) 1999-08-04 2001-12-04 Eastern Virginia Medical School Of The Medical College Of Hampton Roads Method and apparatus for intracellular electro-manipulation
CA2394020C (en) 1999-12-15 2011-04-05 University Of South Florida Electroporation device and method
AU2495801A (en) 1999-12-22 2001-07-09 Innovation And Development Corporation Method and apparatus for targeting localised electroporation
DE10010081A1 (de) 2000-03-02 2001-09-13 Nmi Univ Tuebingen Vorrichtung und Elektrodenanordnung für elektrophysiologische Untersuchungen
US7011939B2 (en) 2000-03-22 2006-03-14 Abbott Laboratories Apparatus and method for electrophysiological testing
GB0013584D0 (en) 2000-06-06 2000-07-26 Cenes Ltd Automated flow patch-clamp system
DE10032568A1 (de) 2000-07-05 2002-01-24 Nmi Univ Tuebingen Vorrichtung und Verfahren zum elektrischen Kontaktieren von in einer Flüssigkeit in Suspension befindlichen biologischen Zellen
EP1178315A1 (de) 2000-07-31 2002-02-06 Albrecht Dr.med. Priv.Doz. Lepple-Wienhues Verfahren und Vorrichtung zur Untersuchung von Zellen mit Hilfe der Patch Clamp-Methode
JP2004512833A (ja) 2000-09-18 2004-04-30 プレジデント・アンド・フェローズ・オブ・ハーバード・カレッジ 単一細胞の選択部分の様々な流体成分での差別的な処理
US6932893B2 (en) 2000-10-02 2005-08-23 Sophion Bioscience A/S System for electrophysiological measurements
US6846306B1 (en) 2000-10-10 2005-01-25 Cold Spring Harbor Laboratory Single cell electroporation
SE0003841D0 (sv) 2000-10-20 2000-10-20 Daniel Chiu A method and apparatus for penetration of lipid bilayer membranes
EP1225216A1 (de) 2001-01-08 2002-07-24 Niels Fertig Vorrichtung zur Untersuchung von Ionenkanälen in Membranen
US7101703B2 (en) 2001-03-09 2006-09-05 Cornell Research Foundation, Inc. Electrofusion microelectrode
WO2002077259A2 (en) 2001-03-24 2002-10-03 Aviva Biosciences Corporation Biochips including ion transport detecting structures and methods of use
US6748266B2 (en) 2001-04-06 2004-06-08 Mattioli Engineering Ltd. Method and apparatus for skin absorption enhancement and transdermal drug delivery
US7127284B2 (en) 2001-06-11 2006-10-24 Mercator Medsystems, Inc. Electroporation microneedle and methods for its use
US6749792B2 (en) 2001-07-09 2004-06-15 Lifescan, Inc. Micro-needles and methods of manufacture and use thereof
DE60217393T2 (de) 2001-11-27 2007-10-18 Cellectricon Ab Verfahren zur kombinierten parallelen zuführung von agentien und elektroporation für zellstrukturen und verwendung davon
US7018819B2 (en) 2001-11-30 2006-03-28 Cellectricon Ab Method and apparatus for manipulation of cells and cell-like structures focused electric fields in microfludic systems and use thereof
US7390650B2 (en) 2002-08-21 2008-06-24 Cellectricon Ab System and method for obtaining and maintaining high-resistance seals in patch clamp recordings
EP2368635A3 (de) 2002-02-12 2012-03-07 Cellectricon Ab Systeme und Verfahren zur schnellen Änderung der Lösungsumgebung um Sensoren
US7470518B2 (en) 2002-02-12 2008-12-30 Cellectricon Ab Systems and method for rapidly changing the solution environment around sensors
AU2003279926A1 (en) 2002-10-09 2004-05-04 Cellectricon Ab Method for interfacing macroscale components to microscale devices
AU2003278461A1 (en) 2002-10-16 2004-05-04 Cellectricon Ab Nanoelectrodes and nanotips for recording transmembrane currents in a plurality of cells
AU2003283663A1 (en) 2002-11-01 2004-05-25 Cellectricon Ab Computer programs,workstations, systems and methods for microfluidic substrates in cell

Also Published As

Publication number Publication date
AU2002353741A1 (en) 2003-06-10
CA2468424A1 (en) 2003-06-05
CA2468511A1 (en) 2003-06-05
JP4842512B2 (ja) 2011-12-21
US20050026283A1 (en) 2005-02-03
AU2002365542A1 (en) 2003-06-10
US8338150B2 (en) 2012-12-25
US20100159439A1 (en) 2010-06-24
JP2005510236A (ja) 2005-04-21
ATE350471T1 (de) 2007-01-15
WO2003046171A1 (en) 2003-06-05
JP2005510237A (ja) 2005-04-21
EP1448771A1 (de) 2004-08-25
US8268555B2 (en) 2012-09-18
US20090092963A1 (en) 2009-04-09
EP1448771B1 (de) 2007-01-03
DE60217393T2 (de) 2007-10-18
WO2003046170A1 (en) 2003-06-05
US20050019921A1 (en) 2005-01-27
EP1448770A1 (de) 2004-08-25

Similar Documents

Publication Publication Date Title
DE60217393D1 (de) Verfahren zur kombinierten parallelen zuführung von agentien und elektroporation für zellstrukturen und verwendung davon
DE60318455D1 (de) Elektrolytischer generator zur herstellung von eluierungsmittel und dessen verwendung
ATE278796T1 (de) Pufferlössung für die elektroporation und verfahren umfassend die verwendung derselben
DE60324126D1 (de) Selektive ausrichtung von nanopartikeln, die wechselstromfelder verwenden
TR200101659T2 (tr) Elektrolitik cihazı, sulu çözeltilerin arıtılması ve kimyasal maddelerin sentezi için metodlar.
DE69835228D1 (de) Verfahren zur elektropermeabilisation einzelner zellulärer und organeller strukturen
US9701954B2 (en) Method and device for uniformly treating adherent cells
MX2010004989A (es) Montaje aislante de doble barra de contacto para extraccion por via electrolitica de un metal, y metodos para su uso.
ATE376454T1 (de) Behältnis und vorrichtung zur erzeugung von elektrischen feldern in einzelnen reaktionsräumen
UA26465C2 (uk) Електрохімічhий пристрій для акумулюваhhя і подаhhя еhергії
ATE421160T1 (de) Mehrschichtige elektrochemische energiespeichereinrichtung und verfahren zu ihrer herstellung
TW431001B (en) Nonaqueous electrolytic secondary battery and manufacture method thereof
ATE460756T1 (de) Polymerverbundmembran mit ionen- /elektronenleitfähigkeit, herstellungsverfahren dafür und kern einer planaren brennstoffzelle mit dieser membran
EP1496012A4 (de) Vorrichtung und verfahren zur herstellung eines leitenden nanodrahtes
DE3576365D1 (de) Elektrode fuer elektrochemische prozesse, verfahren zu deren herstellung und verwendung in elektrolytischen zellen.
BR0109167A (pt) Processo e dispositivo para regulagem da concentração de ìons metálicos em um lìquido eletrolìtico, bem como aplicação do processo e uso do dispositivo
IL158513A0 (en) Circuit arrangement for injecting nucleic acids and other biologically active molecules into the nucleus of higher eucaryontic cells using electrical current
DE502004010612D1 (de) Akkumulator und Verfahren zur Herstellung des selben
WO2016087575A1 (en) High capacity redox electrodes and their use in cell lysis
CN217112169U (zh) 便携式快速生物大分子磁电双作用分离设备
DE69801573D1 (de) Positives elektrodenmaterial auf basis von titanoxysulfid für elektrochemischen generator und verfahren zu dessen herstellung
Yu et al. Quantification of propidium iodide delivery with millisecond electric pulses: a model study
EP1598446A3 (de) Verfahren und Anordnung zum Erfassen von Wechselstromkorrosion an kathodisch geschützten Anlagen
TR200103317A2 (tr) Elektrohemodiyaliz kartuşu.
Mogi et al. Magnetic-field-induced diffusion-limited-aggregation in electrodeposition

Legal Events

Date Code Title Description
8364 No opposition during term of opposition