DE602006018054D1 - Translationselongationsfaktor-promotor aus pichia pastoris und verfahren zur herstellung von rekombinantem protein unter verwendung davon - Google Patents

Translationselongationsfaktor-promotor aus pichia pastoris und verfahren zur herstellung von rekombinantem protein unter verwendung davon

Info

Publication number
DE602006018054D1
DE602006018054D1 DE602006018054T DE602006018054T DE602006018054D1 DE 602006018054 D1 DE602006018054 D1 DE 602006018054D1 DE 602006018054 T DE602006018054 T DE 602006018054T DE 602006018054 T DE602006018054 T DE 602006018054T DE 602006018054 D1 DE602006018054 D1 DE 602006018054D1
Authority
DE
Germany
Prior art keywords
promoter
pichia pastoris
elongation factor
translation elongation
recombinant protein
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
DE602006018054T
Other languages
English (en)
Inventor
Hong Weon Lee
Jung Oh Ahn
Joon Ki Jung
Eui Sung Choi
Chun Suk Kim
Eun Gyo Lee
Ji Yeon Hong
Hyeok Won Lee
Myoung Soo Park
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Korea Research Institute of Bioscience and Biotechnology KRIBB
Original Assignee
Korea Research Institute of Bioscience and Biotechnology KRIBB
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Korea Research Institute of Bioscience and Biotechnology KRIBB filed Critical Korea Research Institute of Bioscience and Biotechnology KRIBB
Publication of DE602006018054D1 publication Critical patent/DE602006018054D1/de
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
    • C07K14/395—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts from Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Mycology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
DE602006018054T 2005-11-16 2006-01-19 Translationselongationsfaktor-promotor aus pichia pastoris und verfahren zur herstellung von rekombinantem protein unter verwendung davon Active DE602006018054D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR20050109697 2005-11-16
PCT/KR2006/000215 WO2007058407A1 (en) 2005-11-16 2006-01-19 Translational elongation factor promoter from pichia pastoris and method for producing recombinant protein using the same

Publications (1)

Publication Number Publication Date
DE602006018054D1 true DE602006018054D1 (de) 2010-12-16

Family

ID=38048774

Family Applications (1)

Application Number Title Priority Date Filing Date
DE602006018054T Active DE602006018054D1 (de) 2005-11-16 2006-01-19 Translationselongationsfaktor-promotor aus pichia pastoris und verfahren zur herstellung von rekombinantem protein unter verwendung davon

Country Status (6)

Country Link
US (1) US7816509B2 (de)
EP (1) EP1951877B1 (de)
KR (1) KR100752107B1 (de)
AT (1) ATE486944T1 (de)
DE (1) DE602006018054D1 (de)
WO (1) WO2007058407A1 (de)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100717353B1 (ko) * 2006-04-12 2007-05-11 한국생명공학연구원 피키아 파스토리스 유래의 자동 유도성 ｎｐｓ 프로모터 및이를 이용한 이종 단백질의 제조방법
CN102088989B (zh) 2008-06-17 2014-11-26 印第安纳大学研究及科技有限公司 在生理pH缓冲液中具有增强的溶解性和稳定性的胰高血糖素类似物
EP2258855A1 (de) 2009-05-28 2010-12-08 Universität für Bodenkultur Wien Expressionssequenzen
ES2913825T3 (es) 2011-10-07 2022-06-06 Lonza Ag Promotor regulable
EP3527667B1 (de) * 2013-03-08 2020-11-25 Biogrammatics, Inc. Hefepromotoren zur proteinexpression
EP2970994B1 (de) 2013-03-15 2019-07-17 Lonza Ltd Konstitutiver promoter
US9150870B2 (en) 2013-03-15 2015-10-06 Lonza Ltd. Constitutive promoter
US20220033832A1 (en) 2018-09-24 2022-02-03 Orta Dogu Teknik Universitesi Modified aox1 promoter variants
CN114774461B (zh) * 2022-04-06 2023-05-26 暨南大学 Ash1p作为负调控因子在提高宿主细胞中蛋白表达中的应用
CN114657197B (zh) * 2022-04-06 2023-07-21 暨南大学 Gsm1p作为正调控因子在提高宿主细胞中蛋白表达中的应用

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5989870A (en) * 1986-04-30 1999-11-23 Rohm Enzyme Finland Oy Method for cloning active promoters
US5650294A (en) * 1990-06-25 1997-07-22 Basf Aktiengesellschaft Isolated promoter and terminator of elongation factor 1-α
US5612198A (en) * 1990-09-04 1997-03-18 The Salk Institute Production of insulin-like growth factor-1 in methylotrophic yeast cells
EP0922109A1 (de) * 1996-05-21 1999-06-16 Novo Nordisk A/S Neue hefepromotoren verwendbar zur expressionsklonierung und zur expression heterologer proteinein hefe
ID23301A (id) 1998-04-23 2000-04-05 Hoffmann La Roche Produksi yang berlebihan dari riboflavin di dalam ragi
US20030064437A1 (en) * 2000-11-15 2003-04-03 Nick Wan Expression system for recombinant proteins
KR100646100B1 (ko) 2004-11-27 2006-11-15 한국생명공학연구원 미생물 유래의 신규 프로모터의 스크리닝 방법

Also Published As

Publication number Publication date
EP1951877B1 (de) 2010-11-03
ATE486944T1 (de) 2010-11-15
KR100752107B1 (ko) 2007-08-24
US7816509B2 (en) 2010-10-19
EP1951877A1 (de) 2008-08-06
EP1951877A4 (de) 2009-02-18
WO2007058407A1 (en) 2007-05-24
US20090298122A1 (en) 2009-12-03
KR20070052179A (ko) 2007-05-21

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