DE602005004354D1 - Verfahren zur Lokalisierung von Fluoreszenzmarkers - Google Patents

Verfahren zur Lokalisierung von Fluoreszenzmarkers

Info

Publication number
DE602005004354D1
DE602005004354D1 DE602005004354T DE602005004354T DE602005004354D1 DE 602005004354 D1 DE602005004354 D1 DE 602005004354D1 DE 602005004354 T DE602005004354 T DE 602005004354T DE 602005004354 T DE602005004354 T DE 602005004354T DE 602005004354 D1 DE602005004354 D1 DE 602005004354D1
Authority
DE
Germany
Prior art keywords
localization
fluorescent marker
marker
fluorescent
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
DE602005004354T
Other languages
English (en)
Other versions
DE602005004354T2 (de
Inventor
Bart Buijsse
Robert F M Hendriks
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Koninklijke Philips NV
FEI Co
Original Assignee
Koninklijke Philips Electronics NV
FEI Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Koninklijke Philips Electronics NV, FEI Co filed Critical Koninklijke Philips Electronics NV
Publication of DE602005004354D1 publication Critical patent/DE602005004354D1/de
Application granted granted Critical
Publication of DE602005004354T2 publication Critical patent/DE602005004354T2/de
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G01N21/6458—Fluorescence microscopy
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N23/00—Investigating or analysing materials by the use of wave or particle radiation, e.g. X-rays or neutrons, not covered by groups G01N3/00 – G01N17/00, G01N21/00 or G01N22/00
    • G01N23/22—Investigating or analysing materials by the use of wave or particle radiation, e.g. X-rays or neutrons, not covered by groups G01N3/00 – G01N17/00, G01N21/00 or G01N22/00 by measuring secondary emission from the material
    • G01N23/225—Investigating or analysing materials by the use of wave or particle radiation, e.g. X-rays or neutrons, not covered by groups G01N3/00 – G01N17/00, G01N21/00 or G01N22/00 by measuring secondary emission from the material using electron or ion
    • H—ELECTRICITY
    • H01—ELECTRIC ELEMENTS
    • H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
    • H01J37/00—Discharge tubes with provision for introducing objects or material to be exposed to the discharge, e.g. for the purpose of examination or processing thereof
    • H01J37/02—Details
    • H01J37/22—Optical, image processing or photographic arrangements associated with the tube
    • H01J37/226—Optical arrangements for illuminating the object; optical arrangements for collecting light from the object
    • H01J37/228—Optical arrangements for illuminating the object; optical arrangements for collecting light from the object whereby illumination or light collection take place in the same area of the discharge
    • H—ELECTRICITY
    • H01—ELECTRIC ELEMENTS
    • H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
    • H01J37/00—Discharge tubes with provision for introducing objects or material to be exposed to the discharge, e.g. for the purpose of examination or processing thereof
    • H01J37/26—Electron or ion microscopes; Electron or ion diffraction tubes
    • H01J37/28—Electron or ion microscopes; Electron or ion diffraction tubes with scanning beams
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6432—Quenching
    • H—ELECTRICITY
    • H01—ELECTRIC ELEMENTS
    • H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
    • H01J2237/00—Discharge tubes exposing object to beam, e.g. for analysis treatment, etching, imaging
    • H01J2237/26—Electron or ion microscopes
    • H01J2237/282—Determination of microscope properties
    • H01J2237/2826—Calibration
    • H—ELECTRICITY
    • H01—ELECTRIC ELEMENTS
    • H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
    • H01J2237/00—Discharge tubes exposing object to beam, e.g. for analysis treatment, etching, imaging
    • H01J2237/30—Electron or ion beam tubes for processing objects
    • H01J2237/304—Controlling tubes
    • H01J2237/30433—System calibration
    • H01J2237/30438—Registration
    • H—ELECTRICITY
    • H01—ELECTRIC ELEMENTS
    • H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
    • H01J2237/00—Discharge tubes exposing object to beam, e.g. for analysis treatment, etching, imaging
    • H01J2237/30—Electron or ion beam tubes for processing objects
    • H01J2237/317—Processing objects on a microscale
    • H01J2237/3174—Etching microareas

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Pathology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Optics & Photonics (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Lasers (AREA)
  • Analysing Materials By The Use Of Radiation (AREA)
DE602005004354T 2004-11-09 2005-11-03 Verfahren zur Lokalisierung von Fluoreszenzmarkers Active DE602005004354T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
NL1027462A NL1027462C2 (nl) 2004-11-09 2004-11-09 Werkwijze voor het lokaliseren van fluorescente markers.
NL1027462 2004-11-09

Publications (2)

Publication Number Publication Date
DE602005004354D1 true DE602005004354D1 (de) 2008-03-06
DE602005004354T2 DE602005004354T2 (de) 2008-05-21

Family

ID=34974505

Family Applications (1)

Application Number Title Priority Date Filing Date
DE602005004354T Active DE602005004354T2 (de) 2004-11-09 2005-11-03 Verfahren zur Lokalisierung von Fluoreszenzmarkers

Country Status (6)

Country Link
US (1) US7317515B2 (de)
EP (1) EP1655597B1 (de)
JP (1) JP4418789B2 (de)
CN (1) CN100565189C (de)
DE (1) DE602005004354T2 (de)
NL (1) NL1027462C2 (de)

Families Citing this family (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1801593A1 (de) * 2005-12-22 2007-06-27 Koninklijke Philips Electronics N.V. Abbildungsmethode von biologischen Proben unter Verwendung von inorganischen Nanopartikeln als Markierungsagentien
EP1890136A1 (de) * 2006-08-16 2008-02-20 FEI Company Verfahren zum Erhalt von Bilderen für Abschnitte einer Probe
JP5873227B2 (ja) * 2007-12-06 2016-03-01 エフ・イ−・アイ・カンパニー デコレーションを用いたスライス・アンド・ビュー
JP2009250904A (ja) * 2008-04-10 2009-10-29 Jeol Ltd 検査装置及び検査方法
US7961397B2 (en) * 2008-08-29 2011-06-14 Omniprobe, Inc Single-channel optical processing system for energetic-beam microscopes
DE102008054148B9 (de) * 2008-10-31 2021-12-30 Hellma Materials Gmbh & Co. Kg Verfahren zur Bestimmung der Laserstabilität von optischem Material sowie damit erhaltene Kristalle und deren Verwendung
JP5492409B2 (ja) * 2008-12-26 2014-05-14 株式会社堀場製作所 電子顕微鏡装置
DE102009015341A1 (de) 2009-03-27 2010-10-07 Carl Zeiss Ag Verfahren und Vorrichtungen zur optischen Untersuchung von Proben
DE102009046211B4 (de) * 2009-10-30 2017-08-24 Carl Zeiss Microscopy Gmbh Detektionsvorrichtung und Teilchenstrahlgerät mit Detektionsvorrichtung
US8350237B2 (en) 2010-03-31 2013-01-08 Fei Company Automated slice milling for viewing a feature
JP5770434B2 (ja) * 2010-06-24 2015-08-26 株式会社堀場製作所 電子顕微鏡装置
NL2005080C2 (en) * 2010-07-14 2012-01-17 Univ Delft Tech Inspection apparatus and replaceable door for a vacuum chamber of such an inspection apparatus.
US8319181B2 (en) 2011-01-30 2012-11-27 Fei Company System and method for localization of large numbers of fluorescent markers in biological samples
EP2509097A1 (de) * 2011-04-07 2012-10-10 FEI Company Verfahren zum Schutz eines Strahlungsdetektors in einem Ladungsteilcheninstrument
US9044781B2 (en) * 2012-12-04 2015-06-02 Fei Company Microfluidics delivery systems
US8872105B2 (en) * 2013-02-19 2014-10-28 Fei Company In situ reactivation of fluorescence marker
DE102013209104A1 (de) 2013-05-16 2014-11-20 Carl Zeiss Microscopy Gmbh Vorrichtung und Verfahren zur spektroskopischen Analyse
NL2012029C2 (en) * 2013-12-24 2015-06-26 Mapper Lithography Ip Bv Charged particle lithography system with sensor assembly.
NL2012218C2 (en) 2014-02-06 2015-08-10 Univ Delft Tech Method and apparatus for determining a density of fluorescent markers in a sample.
KR101725137B1 (ko) * 2015-08-21 2017-04-26 한국표준과학연구원 전자현미경용 시료실 장치 및 이를 구비한 전자현미경
WO2017062060A1 (en) * 2015-10-07 2017-04-13 Intel Corporation In-situ laser assisted processes in focused ion beam applications
EP3159728A1 (de) 2015-10-21 2017-04-26 FEI Company Interferometrisches stehwellenmikroskop
JP2017083234A (ja) * 2015-10-26 2017-05-18 オムロン株式会社 三次元形状計測装置、三次元形状計測システム、プログラム、コンピュータ読み取り可能な記録媒体、および三次元形状計測方法
EP3364248A1 (de) 2017-02-15 2018-08-22 Centre National De La Recherche Scientifique Elektronenstrahllithografieverfahren für eine probe mit mindestens einer zerbrechlichen nanostruktur
KR101917720B1 (ko) 2017-07-31 2019-02-08 한미반도체 주식회사 웨이퍼 마킹 장치 및 웨이퍼 마킹방법
CN111344831B (zh) 2017-11-27 2023-03-24 株式会社日立高新技术 带电粒子束装置以及使用了其的试样观察方法

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2596863B1 (fr) * 1986-04-07 1988-06-17 Centre Nat Rech Scient Dispositif de microscopie analytique, propre a former a la fois une sonde raman et une sonde electronique
DE4233686A1 (de) * 1992-10-02 1994-04-07 Ism Ingenieurbuero Fuer Spezia Verfahren zur selektiven optischen Darstellung bzw. Markierung vorbestimmter Atome, Moleküle oder Molekülgruppen
NL9400111A (nl) * 1994-01-24 1995-09-01 Biomat Res Group Stichting Azl Elektronenmicroscoop met Ramanspectroscopie.
US6259104B1 (en) * 1994-07-15 2001-07-10 Stephen C. Baer Superresolution in optical microscopy and microlithography
US5479024A (en) * 1994-08-11 1995-12-26 The Regents Of The University Of California Method and apparatus for performing near-field optical microscopy
US6002471A (en) * 1996-11-04 1999-12-14 California Institute Of Technology High resolution scanning raman microscope

Also Published As

Publication number Publication date
EP1655597A1 (de) 2006-05-10
NL1027462C2 (nl) 2006-05-10
CN1789982A (zh) 2006-06-21
CN100565189C (zh) 2009-12-02
US7317515B2 (en) 2008-01-08
JP4418789B2 (ja) 2010-02-24
US20060098188A1 (en) 2006-05-11
JP2006145526A (ja) 2006-06-08
EP1655597B1 (de) 2008-01-16
DE602005004354T2 (de) 2008-05-21

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