DE60125156T2 - Nicht-menschliche sperm-dns zubereitungen zur genetischen veränderung nicht-menschlicher tiere - Google Patents
Nicht-menschliche sperm-dns zubereitungen zur genetischen veränderung nicht-menschlicher tiere Download PDFInfo
- Publication number
- DE60125156T2 DE60125156T2 DE60125156T DE60125156T DE60125156T2 DE 60125156 T2 DE60125156 T2 DE 60125156T2 DE 60125156 T DE60125156 T DE 60125156T DE 60125156 T DE60125156 T DE 60125156T DE 60125156 T2 DE60125156 T2 DE 60125156T2
- Authority
- DE
- Germany
- Prior art keywords
- antibody
- dna
- sperm cells
- sperm
- linker
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 241001465754 Metazoa Species 0.000 title description 26
- 230000002068 genetic effect Effects 0.000 title description 3
- 238000002360 preparation method Methods 0.000 title description 2
- 108020004414 DNA Proteins 0.000 claims description 64
- 108090000623 proteins and genes Proteins 0.000 claims description 28
- 239000013598 vector Substances 0.000 claims description 19
- 230000003993 interaction Effects 0.000 claims description 15
- 108091033319 polynucleotide Proteins 0.000 claims description 12
- 102000040430 polynucleotide Human genes 0.000 claims description 12
- 239000002157 polynucleotide Substances 0.000 claims description 12
- 239000002502 liposome Substances 0.000 claims description 8
- 238000012239 gene modification Methods 0.000 claims description 7
- 230000005017 genetic modification Effects 0.000 claims description 7
- 235000013617 genetically modified food Nutrition 0.000 claims description 7
- 102000004169 proteins and genes Human genes 0.000 claims description 7
- 230000004001 molecular interaction Effects 0.000 claims description 4
- 102000053602 DNA Human genes 0.000 claims 2
- 210000004027 cell Anatomy 0.000 description 91
- 238000000034 method Methods 0.000 description 23
- 239000013612 plasmid Substances 0.000 description 23
- 241000282887 Suidae Species 0.000 description 18
- 241000699666 Mus <mouse, genus> Species 0.000 description 13
- 230000004720 fertilization Effects 0.000 description 12
- 241000287828 Gallus gallus Species 0.000 description 11
- 230000027455 binding Effects 0.000 description 11
- 241000699670 Mus sp. Species 0.000 description 10
- 238000006243 chemical reaction Methods 0.000 description 10
- 241000283707 Capra Species 0.000 description 9
- 238000000338 in vitro Methods 0.000 description 9
- 241000282898 Sus scrofa Species 0.000 description 8
- 238000001727 in vivo Methods 0.000 description 8
- 238000004519 manufacturing process Methods 0.000 description 8
- 230000009261 transgenic effect Effects 0.000 description 8
- 241000283690 Bos taurus Species 0.000 description 7
- 108091007433 antigens Proteins 0.000 description 7
- 230000000694 effects Effects 0.000 description 7
- 238000000684 flow cytometry Methods 0.000 description 7
- 239000000203 mixture Substances 0.000 description 7
- 102000002260 Alkaline Phosphatase Human genes 0.000 description 6
- 108020004774 Alkaline Phosphatase Proteins 0.000 description 6
- 241001494479 Pecora Species 0.000 description 6
- 239000000427 antigen Substances 0.000 description 6
- 102000036639 antigens Human genes 0.000 description 6
- 210000002257 embryonic structure Anatomy 0.000 description 6
- 210000000287 oocyte Anatomy 0.000 description 6
- 108091029865 Exogenous DNA Proteins 0.000 description 5
- 108060003951 Immunoglobulin Proteins 0.000 description 5
- 238000002105 Southern blotting Methods 0.000 description 5
- 239000000499 gel Substances 0.000 description 5
- 230000012010 growth Effects 0.000 description 5
- 102000018358 immunoglobulin Human genes 0.000 description 5
- 101710163270 Nuclease Proteins 0.000 description 4
- 238000001514 detection method Methods 0.000 description 4
- 235000013601 eggs Nutrition 0.000 description 4
- 235000018102 proteins Nutrition 0.000 description 4
- 210000001550 testis Anatomy 0.000 description 4
- 210000001519 tissue Anatomy 0.000 description 4
- 230000004568 DNA-binding Effects 0.000 description 3
- 108010035533 Drosophila Proteins Proteins 0.000 description 3
- 102000004190 Enzymes Human genes 0.000 description 3
- 108090000790 Enzymes Proteins 0.000 description 3
- 241000124008 Mammalia Species 0.000 description 3
- 108091000054 Prion Proteins 0.000 description 3
- 102000029797 Prion Human genes 0.000 description 3
- 108010090804 Streptavidin Proteins 0.000 description 3
- 239000003795 chemical substances by application Substances 0.000 description 3
- 210000000349 chromosome Anatomy 0.000 description 3
- 238000004520 electroporation Methods 0.000 description 3
- 230000002779 inactivation Effects 0.000 description 3
- 208000015181 infectious disease Diseases 0.000 description 3
- 238000002347 injection Methods 0.000 description 3
- 239000007924 injection Substances 0.000 description 3
- 150000002632 lipids Chemical class 0.000 description 3
- 244000144972 livestock Species 0.000 description 3
- 238000000520 microinjection Methods 0.000 description 3
- 210000003101 oviduct Anatomy 0.000 description 3
- 244000052769 pathogen Species 0.000 description 3
- 210000000582 semen Anatomy 0.000 description 3
- 210000002966 serum Anatomy 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- 238000012546 transfer Methods 0.000 description 3
- 210000002268 wool Anatomy 0.000 description 3
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 2
- 102000011022 Chorionic Gonadotropin Human genes 0.000 description 2
- 108010062540 Chorionic Gonadotropin Proteins 0.000 description 2
- 238000011771 FVB mouse Methods 0.000 description 2
- 241000238631 Hexapoda Species 0.000 description 2
- 241000581650 Ivesia Species 0.000 description 2
- 108091036060 Linker DNA Proteins 0.000 description 2
- 239000004677 Nylon Substances 0.000 description 2
- 238000010222 PCR analysis Methods 0.000 description 2
- 238000005411 Van der Waals force Methods 0.000 description 2
- ZSLZBFCDCINBPY-ZSJPKINUSA-N acetyl-CoA Chemical compound O[C@@H]1[C@H](OP(O)(O)=O)[C@@H](COP(O)(=O)OP(O)(=O)OCC(C)(C)[C@@H](O)C(=O)NCCC(=O)NCCSC(=O)C)O[C@H]1N1C2=NC=NC(N)=C2N=C1 ZSLZBFCDCINBPY-ZSJPKINUSA-N 0.000 description 2
- 239000011543 agarose gel Substances 0.000 description 2
- 235000001014 amino acid Nutrition 0.000 description 2
- 150000001413 amino acids Chemical class 0.000 description 2
- 238000003556 assay Methods 0.000 description 2
- 230000005540 biological transmission Effects 0.000 description 2
- 230000000295 complement effect Effects 0.000 description 2
- 230000008878 coupling Effects 0.000 description 2
- 238000010168 coupling process Methods 0.000 description 2
- 238000005859 coupling reaction Methods 0.000 description 2
- 235000018417 cysteine Nutrition 0.000 description 2
- XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 description 2
- 210000000918 epididymis Anatomy 0.000 description 2
- 201000010063 epididymitis Diseases 0.000 description 2
- 239000012634 fragment Substances 0.000 description 2
- 208000002672 hepatitis B Diseases 0.000 description 2
- 229940084986 human chorionic gonadotropin Drugs 0.000 description 2
- 238000009396 hybridization Methods 0.000 description 2
- 238000011534 incubation Methods 0.000 description 2
- 230000002458 infectious effect Effects 0.000 description 2
- 230000009027 insemination Effects 0.000 description 2
- 230000010354 integration Effects 0.000 description 2
- 230000001404 mediated effect Effects 0.000 description 2
- 239000012528 membrane Substances 0.000 description 2
- 235000013336 milk Nutrition 0.000 description 2
- 210000004080 milk Anatomy 0.000 description 2
- 239000008267 milk Substances 0.000 description 2
- 229920001778 nylon Polymers 0.000 description 2
- 244000144977 poultry Species 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 230000008707 rearrangement Effects 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 230000035899 viability Effects 0.000 description 2
- 230000003612 virological effect Effects 0.000 description 2
- 238000002689 xenotransplantation Methods 0.000 description 2
- MTCFGRXMJLQNBG-REOHCLBHSA-N (2S)-2-Amino-3-hydroxypropansäure Chemical compound OC[C@H](N)C(O)=O MTCFGRXMJLQNBG-REOHCLBHSA-N 0.000 description 1
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 1
- 102000040650 (ribonucleotides)n+m Human genes 0.000 description 1
- 241000251468 Actinopterygii Species 0.000 description 1
- 108020005544 Antisense RNA Proteins 0.000 description 1
- 241000271566 Aves Species 0.000 description 1
- 108700003860 Bacterial Genes Proteins 0.000 description 1
- 108010077805 Bacterial Proteins Proteins 0.000 description 1
- 108020004513 Bacterial RNA Proteins 0.000 description 1
- 241000255789 Bombyx mori Species 0.000 description 1
- 101710109085 Cysteine synthase, chloroplastic/chromoplastic Proteins 0.000 description 1
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 1
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 1
- 208000035240 Disease Resistance Diseases 0.000 description 1
- 108700039691 Genetic Promoter Regions Proteins 0.000 description 1
- 102000018997 Growth Hormone Human genes 0.000 description 1
- 108010051696 Growth Hormone Proteins 0.000 description 1
- 241000282412 Homo Species 0.000 description 1
- 102000014150 Interferons Human genes 0.000 description 1
- 108010050904 Interferons Proteins 0.000 description 1
- 241001529936 Murinae Species 0.000 description 1
- 241000699660 Mus musculus Species 0.000 description 1
- 108091061960 Naked DNA Proteins 0.000 description 1
- 101710138316 O-acetylserine sulfhydrylase Proteins 0.000 description 1
- 206010034133 Pathogen resistance Diseases 0.000 description 1
- 208000027954 Poultry disease Diseases 0.000 description 1
- 241000607142 Salmonella Species 0.000 description 1
- MTCFGRXMJLQNBG-UHFFFAOYSA-N Serine Natural products OCC(N)C(O)=O MTCFGRXMJLQNBG-UHFFFAOYSA-N 0.000 description 1
- 108091022908 Serine O-acetyltransferase Proteins 0.000 description 1
- 108700019146 Transgenes Proteins 0.000 description 1
- 108010067390 Viral Proteins Proteins 0.000 description 1
- 208000036142 Viral infection Diseases 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 238000005281 X-ray Gandolfi Methods 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 210000000577 adipose tissue Anatomy 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 238000003975 animal breeding Methods 0.000 description 1
- 210000001557 animal structure Anatomy 0.000 description 1
- 235000015278 beef Nutrition 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 229960002685 biotin Drugs 0.000 description 1
- 235000020958 biotin Nutrition 0.000 description 1
- 239000011616 biotin Substances 0.000 description 1
- 210000001109 blastomere Anatomy 0.000 description 1
- 230000032823 cell division Effects 0.000 description 1
- 230000009087 cell motility Effects 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 239000013611 chromosomal DNA Substances 0.000 description 1
- 230000002759 chromosomal effect Effects 0.000 description 1
- 239000003184 complementary RNA Substances 0.000 description 1
- 230000001268 conjugating effect Effects 0.000 description 1
- 230000027326 copulation Effects 0.000 description 1
- 230000009260 cross reactivity Effects 0.000 description 1
- 210000001771 cumulus cell Anatomy 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 230000005684 electric field Effects 0.000 description 1
- 210000001671 embryonic stem cell Anatomy 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000001952 enzyme assay Methods 0.000 description 1
- ZMMJGEGLRURXTF-UHFFFAOYSA-N ethidium bromide Chemical compound [Br-].C12=CC(N)=CC=C2C2=CC=C(N)C=C2[N+](CC)=C1C1=CC=CC=C1 ZMMJGEGLRURXTF-UHFFFAOYSA-N 0.000 description 1
- 229960005542 ethidium bromide Drugs 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 230000008020 evaporation Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 208000010824 fish disease Diseases 0.000 description 1
- GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 description 1
- 238000001943 fluorescence-activated cell sorting Methods 0.000 description 1
- 230000006870 function Effects 0.000 description 1
- 239000000122 growth hormone Substances 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 210000004408 hybridoma Anatomy 0.000 description 1
- 210000000987 immune system Anatomy 0.000 description 1
- 229940127121 immunoconjugate Drugs 0.000 description 1
- 229940072221 immunoglobulins Drugs 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 229940079322 interferon Drugs 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 210000004962 mammalian cell Anatomy 0.000 description 1
- 210000001161 mammalian embryo Anatomy 0.000 description 1
- 239000003550 marker Substances 0.000 description 1
- 239000002480 mineral oil Substances 0.000 description 1
- 235000010446 mineral oil Nutrition 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 230000036961 partial effect Effects 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 230000002688 persistence Effects 0.000 description 1
- 230000000384 rearing effect Effects 0.000 description 1
- 230000006798 recombination Effects 0.000 description 1
- 238000005215 recombination Methods 0.000 description 1
- 230000002829 reductive effect Effects 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 238000012552 review Methods 0.000 description 1
- PYWVYCXTNDRMGF-UHFFFAOYSA-N rhodamine B Chemical compound [Cl-].C=12C=CC(=[N+](CC)CC)C=C2OC2=CC(N(CC)CC)=CC=C2C=1C1=CC=CC=C1C(O)=O PYWVYCXTNDRMGF-UHFFFAOYSA-N 0.000 description 1
- 239000000523 sample Substances 0.000 description 1
- 238000003345 scintillation counting Methods 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 238000011830 transgenic mouse model Methods 0.000 description 1
- 238000013519 translation Methods 0.000 description 1
- 238000002054 transplantation Methods 0.000 description 1
- 241000712461 unidentified influenza virus Species 0.000 description 1
- 230000009385 viral infection Effects 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/027—New or modified breeds of vertebrates
- A01K67/0275—Genetically modified vertebrates, e.g. transgenic
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
- A61K47/69—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the conjugate being characterised by physical or galenical forms, e.g. emulsion, particle, inclusion complex, stent or kit
- A61K47/6901—Conjugates being cells, cell fragments, viruses, ghosts, red blood cells or viral vectors
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
- C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/0006—Modification of the membrane of cells, e.g. cell decoration
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0608—Germ cells
- C12N5/061—Sperm cells, spermatogonia
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2217/00—Genetically modified animals
- A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K2039/505—Medicinal preparations containing antigens or antibodies comprising antibodies
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2510/00—Genetically modified cells
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Molecular Biology (AREA)
- Animal Behavior & Ethology (AREA)
- Medicinal Chemistry (AREA)
- Biophysics (AREA)
- Immunology (AREA)
- Environmental Sciences (AREA)
- Veterinary Medicine (AREA)
- Virology (AREA)
- Reproductive Health (AREA)
- Pharmacology & Pharmacy (AREA)
- Public Health (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Epidemiology (AREA)
- Developmental Biology & Embryology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Hematology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Materials For Medical Uses (AREA)
- Peptides Or Proteins (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US537861 | 2000-03-28 | ||
| US09/537,861 US7067308B1 (en) | 2000-03-28 | 2000-03-28 | Vector for genetically modifying non-human animals |
| PCT/US2001/007018 WO2001073094A2 (en) | 2000-03-28 | 2001-03-05 | Novel sperm-dna complexes for genetically modifying non-human animals |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DE60125156D1 DE60125156D1 (de) | 2007-01-25 |
| DE60125156T2 true DE60125156T2 (de) | 2007-09-20 |
Family
ID=24144410
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE60125156T Expired - Lifetime DE60125156T2 (de) | 2000-03-28 | 2001-03-05 | Nicht-menschliche sperm-dns zubereitungen zur genetischen veränderung nicht-menschlicher tiere |
Country Status (10)
| Country | Link |
|---|---|
| US (2) | US7067308B1 (enExample) |
| EP (2) | EP1760155A3 (enExample) |
| JP (1) | JP2003528617A (enExample) |
| CN (1) | CN1432067A (enExample) |
| AR (1) | AR028298A1 (enExample) |
| AT (1) | ATE348184T1 (enExample) |
| AU (1) | AU2001245438A1 (enExample) |
| DE (1) | DE60125156T2 (enExample) |
| PE (1) | PE20011214A1 (enExample) |
| WO (1) | WO2001073094A2 (enExample) |
Families Citing this family (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7053187B2 (en) | 2000-03-28 | 2006-05-30 | Gioagri Corporation | Sperm-specific monoclonal antibody, mAbC |
| US7067308B1 (en) * | 2000-03-28 | 2006-06-27 | Bioagri Corporation | Vector for genetically modifying non-human animals |
| US7527966B2 (en) | 2002-06-26 | 2009-05-05 | Transgenrx, Inc. | Gene regulation in transgenic animals using a transposon-based vector |
| NZ597601A (en) | 2007-05-16 | 2013-04-26 | Mat Malta Advanced Technologies Ltd | Treatment and prevention of influenza |
| WO2010036976A2 (en) | 2008-09-25 | 2010-04-01 | Transgenrx, Inc. | Novel vectors for production of antibodies |
| WO2010036978A2 (en) | 2008-09-25 | 2010-04-01 | Transgenrx, Inc. | Novel vectors for production of growth hormone |
| EP2417249B1 (en) * | 2009-04-08 | 2022-06-08 | The Regents of The University of California | Dna-cell conjugates |
| EP2417263B1 (en) | 2009-04-09 | 2015-09-23 | ProteoVec Holding L.L.C. | Production of proteins using transposon-based vectors |
| CN102453720B (zh) * | 2010-10-28 | 2013-01-30 | 华中农业大学 | 一种在猪肌肉组织高效表达的融合启动子 |
| SG10201510056SA (en) * | 2011-10-28 | 2016-01-28 | Regeneron Pharma | Genetically modified mice expressing chimeric major histocompatibility complex (mhc) ii molecules |
| US20150024497A1 (en) * | 2013-07-19 | 2015-01-22 | Elwha Llc | Methods and Systems for Utilizing Sperm for Molecular Delivery |
| BR112019025358A2 (pt) | 2017-05-31 | 2020-07-07 | Commonwealth Scientific And Industrial Research Organisation | ovo aviário transgênico, ave transgênica, ovo ou progênie aviária, método para detectar um ovo aviário macho, método para produzir um ovo aviário, método para replicar um vírus, vírus produzidos pelo uso de ovo aviário, método para produzir uma composição de vacina, composição da vacina produzida pelo uso do método e método para produzir um ovo aviário transgênico ou uma ave produzida pelo ovo |
Family Cites Families (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5428132A (en) | 1987-10-11 | 1995-06-27 | United States Of America | Conjugate and method for integration of foreign DNA into cells |
| IT1228210B (it) | 1989-01-10 | 1991-06-05 | Consiglio Nazionale Ricerche | Procedimento per la introduzione di dna esogeno nelle cellule somatiche e germinali di animali. |
| IL92529A0 (en) | 1989-12-03 | 1990-08-31 | Yissum Res Dev Co | Generation of transgenic vertebrates by employing transformed sperm cells via artificial insemination |
| US5521291A (en) | 1991-09-30 | 1996-05-28 | Boehringer Ingelheim International, Gmbh | Conjugates for introducing nucleic acid into higher eucaryotic cells |
| US6063630A (en) * | 1991-11-05 | 2000-05-16 | Transkaryotic Therapies, Inc. | Targeted introduction of DNA into primary or secondary cells and their use for gene therapy |
| AU2146092A (en) | 1992-05-28 | 1993-12-30 | Scientific Dimensions Usa, Inc. | Transgenic animal production with biolistically transformed spermatozoa |
| RU2081914C1 (ru) | 1994-07-21 | 1997-06-20 | Андрей Вадимович Кузнецов | Способ введения чужеродной днк в сперматозоиды |
| US5744335A (en) | 1995-09-19 | 1998-04-28 | Mirus Corporation | Process of transfecting a cell with a polynucleotide mixed with an amphipathic compound and a DNA-binding protein |
| JPH10304790A (ja) | 1995-09-29 | 1998-11-17 | Hoechst Japan Ltd | トランスジェニック動物の作成方法 |
| CN1112165C (zh) | 1997-08-15 | 2003-06-25 | 中国农业大学 | 精子介dna转移生产转基因动物的方法 |
| WO1999038991A1 (en) | 1998-01-28 | 1999-08-05 | Takara Shuzo Co., Ltd. | Method for transferring gene into germ cell |
| NZ506341A (en) | 1998-02-09 | 2003-02-28 | Tranxenogen Inc | Genetic manipulation of spermatogonia |
| IL123411A0 (en) | 1998-02-22 | 1998-09-24 | Kimron Veterinary Inst | High efficiency methods and compositions for integrating exogenous dna into genomic dna of sperm |
| JP2002524054A (ja) | 1998-08-11 | 2002-08-06 | アンソニー・シー・エフ・ペリー | 遺伝子転移を行う方法 |
| US7067308B1 (en) * | 2000-03-28 | 2006-06-27 | Bioagri Corporation | Vector for genetically modifying non-human animals |
| US7053187B2 (en) * | 2000-03-28 | 2006-05-30 | Gioagri Corporation | Sperm-specific monoclonal antibody, mAbC |
-
2000
- 2000-03-28 US US09/537,861 patent/US7067308B1/en not_active Expired - Fee Related
-
2001
- 2001-03-05 AT AT01918350T patent/ATE348184T1/de not_active IP Right Cessation
- 2001-03-05 EP EP06125927A patent/EP1760155A3/en not_active Withdrawn
- 2001-03-05 EP EP01918350A patent/EP1268836B1/en not_active Expired - Lifetime
- 2001-03-05 DE DE60125156T patent/DE60125156T2/de not_active Expired - Lifetime
- 2001-03-05 WO PCT/US2001/007018 patent/WO2001073094A2/en not_active Ceased
- 2001-03-05 AU AU2001245438A patent/AU2001245438A1/en not_active Abandoned
- 2001-03-05 JP JP2001570810A patent/JP2003528617A/ja active Pending
- 2001-03-05 CN CN01810496A patent/CN1432067A/zh active Pending
- 2001-03-27 PE PE2001000287A patent/PE20011214A1/es not_active Application Discontinuation
- 2001-03-28 AR ARP010101471A patent/AR028298A1/es unknown
-
2006
- 2006-04-20 US US11/407,851 patent/US20070214512A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| ATE348184T1 (de) | 2007-01-15 |
| EP1268836A2 (en) | 2003-01-02 |
| PE20011214A1 (es) | 2001-11-29 |
| WO2001073094A3 (en) | 2002-05-30 |
| JP2003528617A (ja) | 2003-09-30 |
| AU2001245438A1 (en) | 2001-10-08 |
| US20070214512A1 (en) | 2007-09-13 |
| CN1432067A (zh) | 2003-07-23 |
| EP1760155A3 (en) | 2007-03-14 |
| EP1268836B1 (en) | 2006-12-13 |
| EP1760155A2 (en) | 2007-03-07 |
| US7067308B1 (en) | 2006-06-27 |
| WO2001073094A2 (en) | 2001-10-04 |
| DE60125156D1 (de) | 2007-01-25 |
| AR028298A1 (es) | 2003-04-30 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US11230697B2 (en) | Enhanced expression of human or humanized immunoglobulin in non-human transgenic animals | |
| US5523222A (en) | Polyelectrolyte DNA conjugation and genetic transformation of an animal | |
| Chang et al. | Effective generation of transgenic pigs and mice by linker based sperm-mediated gene transfer. | |
| DE60125156T2 (de) | Nicht-menschliche sperm-dns zubereitungen zur genetischen veränderung nicht-menschlicher tiere | |
| EP0665883A1 (en) | Tetracycline repressor-mediated binary regulation system for control of gene expression in transgenic animals | |
| DE60023451T2 (de) | Aus nicht-menschlichen transgenen tieren gewonnene menschliche polyklonale antikörper | |
| WO1994004672A9 (en) | Tetracycline repressor-mediated binary regulation system for control of gene expression in transgenic animals | |
| DE69627885T2 (de) | Methode zur entwicklung transgener ziegen | |
| WO1999036510A1 (de) | Effizienter kerntransfer mit fetalen fibroblasten | |
| CN101155509A (zh) | 转基因鸡 | |
| US20170218336A1 (en) | Enhanced expression of human or humanized immunoglobulin in non-human transgenic animals | |
| DE69600719T2 (de) | Vorbereitung transgenischer Proteine | |
| AU673990B2 (en) | Repopulation of testicular seminiferous tubules with foreign cells | |
| US20020162134A1 (en) | Primordial germ cell-based germ line production of birds | |
| EP1361788A2 (en) | Primordial germ cell-based germ line production of birds | |
| RU2402211C2 (ru) | Способ получения трансгенных кроликов, продуцирующих белки в молочную железу | |
| US20020194638A1 (en) | Vector for genetically modifying non-human animals | |
| Arima et al. | Possible Transfer of Intra-Testicularly Injected Foreign DNA to Generations in Chicken | |
| Velander | Polyelectrolyte DNA conjugation and genetic transformation of an animal | |
| Gordon | Modification of the germ line in animals | |
| Wall et al. | Alteration of the genome as a method to increase diversity in animal germplasm resources | |
| DE19607367A1 (de) | Herstellung von transgenem Geflügel durch Gentransfer mit p95-spezifischen Genfähren | |
| ROBERT | Alteration of the genome as a method to increase diversity in | |
| INTRA | Animal Resource Science Section, Division of Bioresource and Bioenvironmental Sciences, Graduate School, Kyushu University | |
| King | Genetic engineering, chromosome analysis and sex determination |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 8364 | No opposition during term of opposition | ||
| 8327 | Change in the person/name/address of the patent owner |
Owner name: KWANG HUA DEVELOPMENT AND INVESTMENT, LTD., WA, HK |
|
| 8328 | Change in the person/name/address of the agent |
Representative=s name: BETTEN & RESCH, 80333 MUENCHEN |