DE60112282D1 - Auf rezeptorgrundlage arbeitende screening-verfahren für protein wechselwirkungen - Google Patents

Auf rezeptorgrundlage arbeitende screening-verfahren für protein wechselwirkungen

Info

Publication number
DE60112282D1
DE60112282D1 DE60112282T DE60112282T DE60112282D1 DE 60112282 D1 DE60112282 D1 DE 60112282D1 DE 60112282 T DE60112282 T DE 60112282T DE 60112282 T DE60112282 T DE 60112282T DE 60112282 D1 DE60112282 D1 DE 60112282D1
Authority
DE
Germany
Prior art keywords
protein interactions
binding
screening procedures
receptor based
based working
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60112282T
Other languages
English (en)
Other versions
DE60112282T2 (de
Inventor
Sven Eyckerman
Ostade Xaveer Van
Joel Vandekerckhove
Annick Verhee
Jan Tavernier
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Vlaams Instituut voor Biotechnologie VIB
Original Assignee
Vlaams Instituut voor Biotechnologie VIB
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Vlaams Instituut voor Biotechnologie VIB filed Critical Vlaams Instituut voor Biotechnologie VIB
Publication of DE60112282D1 publication Critical patent/DE60112282D1/de
Application granted granted Critical
Publication of DE60112282T2 publication Critical patent/DE60112282T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1055—Protein x Protein interaction, e.g. two hybrid selection
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/71—Receptors; Cell surface antigens; Cell surface determinants for growth factors; for growth regulators
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/715—Receptors; Cell surface antigens; Cell surface determinants for cytokines; for lymphokines; for interferons
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/90—Fusion polypeptide containing a motif for post-translational modification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2799/00—Uses of viruses
    • C12N2799/02—Uses of viruses as vector
    • C12N2799/021—Uses of viruses as vector for the expression of a heterologous nucleic acid
    • C12N2799/027—Uses of viruses as vector for the expression of a heterologous nucleic acid where the vector is derived from a retrovirus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2799/00—Uses of viruses
    • C12N2799/02—Uses of viruses as vector
    • C12N2799/06—Uses of viruses as vector in vitro
DE60112282T 2000-05-22 2001-05-22 Auf rezeptorgrundlage arbeitende screening-verfahren für protein wechselwirkungen Expired - Lifetime DE60112282T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP00201771 2000-05-22
EP00201771 2000-05-22
PCT/EP2001/005916 WO2001090188A2 (en) 2000-05-22 2001-05-22 Receptor-based interaction trap

Publications (2)

Publication Number Publication Date
DE60112282D1 true DE60112282D1 (de) 2005-09-01
DE60112282T2 DE60112282T2 (de) 2006-04-20

Family

ID=8171515

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60112282T Expired - Lifetime DE60112282T2 (de) 2000-05-22 2001-05-22 Auf rezeptorgrundlage arbeitende screening-verfahren für protein wechselwirkungen

Country Status (8)

Country Link
US (2) US7855270B2 (de)
EP (2) EP1283878B1 (de)
JP (1) JP4756813B2 (de)
AT (1) ATE300611T1 (de)
AU (2) AU8178401A (de)
CA (1) CA2407872C (de)
DE (1) DE60112282T2 (de)
WO (1) WO2001090188A2 (de)

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* Cited by examiner, † Cited by third party
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ATE497603T1 (de) 2001-03-02 2011-02-15 Gpc Biotech Ag Drei-hybrid-assaysystem
WO2003004643A2 (en) 2001-07-03 2003-01-16 Vlaams Interuniversitair Instituut Voor Biotechnologie Vzw Reversed mammalian protein-protein interaction trap
GB201103453D0 (en) * 2011-03-01 2011-04-13 Vib Vzw Kinase substrate sensor
US9746470B2 (en) * 2011-05-09 2017-08-29 Whitehead Institute For Biomedical Research Chaperone interaction assays and uses thereof
CA2866097C (en) 2012-03-06 2021-02-02 Vib Vzw Membrane span-kinase fusion protein and the uses thereof
US10444245B2 (en) 2012-05-24 2019-10-15 Vib Vzw Trapping mammalian protein-protein complexes in virus-like particles utilizing HIV-1 GAG-bait fusion proteins
GB201504859D0 (en) 2015-03-23 2015-05-06 Vib Vzw Viral particle based small molecule-protein interaction trap
GB201909491D0 (en) * 2019-07-01 2019-08-14 Governing Council Of The Univ Of Toronto Detection of protein to protein interactions
CA3162256A1 (en) * 2019-12-17 2021-06-24 Nikolai Kley Detection of molecular interactions
AU2020408339A1 (en) * 2019-12-17 2022-07-14 Orionis Biosciences BV Detection of novel degradation-related interactions
AU2022349077A1 (en) * 2021-09-22 2024-03-28 Sonoma Biotherapeutics, Inc. Il5ra cell surface markers

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JP3771253B2 (ja) 1988-09-02 2006-04-26 ダイアックス コープ． 新規な結合タンパク質の生成と選択
WO1992020791A1 (en) 1990-07-10 1992-11-26 Cambridge Antibody Technology Limited Methods for producing members of specific binding pairs
EP0804561B1 (de) 1993-02-12 2009-12-30 The Board Of Trustees Of The Leland Stanford Junior University Regulierte transkription von zielgerichteten genen und andere biologische ereignisse
US5521066A (en) 1993-09-13 1996-05-28 Bristol-Myers Squibb Company Periplasmic membrane-bound system for detecting protein-protein interactions
US5935797A (en) * 1994-06-16 1999-08-10 Stanford University Interaction of MHC Class II proteins with members of the PCNA family of proteins
US5716622A (en) * 1995-01-06 1998-02-10 The Rockefeller University Functionally active regions of signal transducer and activators of transcription
US5712149A (en) * 1995-02-03 1998-01-27 Cell Genesys, Inc. Chimeric receptor molecules for delivery of co-stimulatory signals
US5637463A (en) 1995-05-04 1997-06-10 Hoffmann-La Roche Inc. Method to detect protein-protein interactions
GB9511935D0 (en) 1995-06-13 1995-08-09 Smithkline Beecham Plc Novel compound
SE9503151D0 (sv) 1995-09-13 1995-09-13 Bioinvent Internatioal Ab Combined ligand and receptor display
US5972621A (en) 1995-11-27 1999-10-26 Millennium Pharmaceuticals, Inc. Methods of identifying compounds that modulate body weight using the OB receptor
WO1997020933A2 (en) 1995-12-06 1997-06-12 Schering Corporation MUTATIONAL VARIANTS OF MAMMALIAN Ob GENE PROTEINS
US7063958B1 (en) 1996-01-16 2006-06-20 The Rockefeller University Nucleic acids db, the receptor for leptin
JP2000506007A (ja) * 1996-02-23 2000-05-23 アリアド・ファーマシューティカルズ・インコーポレイテッド 細胞によるアッセイ
WO1997032017A1 (en) 1996-02-26 1997-09-04 Morphosys Gesellschaft Für Proteinoptimierung Mbh Novel method for the identification of nucleic acid sequences encoding two or more interacting (poly)peptides
EP0912609A2 (de) 1996-06-06 1999-05-06 Smithkline Beecham Plc Leptinfragmente (ob protein)
US6001816A (en) 1996-06-20 1999-12-14 Merck & Co., Inc. Gene therapy for leptin deficiency
US5843697A (en) * 1996-07-17 1998-12-01 University Of Medicine And Dentistry Of New Jersey Cells expressing IL-10 receptor and the CRFB4 gene product, an IL-10 receptor accessory protein
US5776689A (en) * 1996-07-19 1998-07-07 The Regents Of The University Of California Protein recruitment system
WO1998012224A1 (en) 1996-09-20 1998-03-26 Hoechst Aktiengesellschaft Use of leptin antagonists for treating insulin resistance in type ii diabetes
AU4789297A (en) 1996-11-01 1998-05-29 Smithkline Beecham Plc Method for the detection of compounds that modulate the effects of the obese (ob) protein
US6294330B1 (en) 1997-01-31 2001-09-25 Odyssey Pharmaceuticals Inc. Protein fragment complementation assays for the detection of biological or drug interactions
CA2196496A1 (en) 1997-01-31 1998-07-31 Stephen William Watson Michnick Protein fragment complementation assay for the detection of protein-protein interactions
US6342345B1 (en) * 1997-04-02 2002-01-29 The Board Of Trustees Of The Leland Stanford Junior University Detection of molecular interactions by reporter subunit complementation
WO1999003974A1 (en) * 1997-07-15 1999-01-28 The Regents Of The University Of California Compositions and methods for identifying modulators of transducisomes, a new class of therapeutic targets
US5885779A (en) * 1997-09-09 1999-03-23 University Of British Columbia Repressed trans-activator system for characterization of protein-protein interactions
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AU765010B2 (en) 1998-07-28 2003-09-04 Vlaams Interuniversitair Instituut Voor Biotechnologie Vzw Eukaryotic cell-based gene interaction cloning
CA2335107C (en) 1998-07-28 2010-01-19 Vlaams Interuniversitair Instituut Voor Biotechnologie Vzw Leptin-mediated gene-induction
AU5143099A (en) * 1998-07-30 2000-02-21 Universite De Montreal Protein fragment complementation assays for the detection of biological or drug interactions
WO2001096538A2 (en) 2000-06-13 2001-12-20 Bayer Aktiengesellschaft Regulation of human transmembrane serine protease
AU2067202A (en) 2000-11-14 2002-05-27 Vlaams Interuniv Inst Biotech Functional fragment of the lectine receptor
HUP0402071A2 (hu) 2001-02-06 2005-01-28 Merck Patent Gmbh. Csökkentett immunogenitású, módosított leptin
EP1812038A1 (de) * 2004-11-18 2007-08-01 VIB vzw Fibronectin iii domäne als leptin-rezeptor-antagonisten

Also Published As

Publication number Publication date
WO2001090188A2 (en) 2001-11-29
EP1283878A2 (de) 2003-02-19
US8003757B2 (en) 2011-08-23
EP1612221A3 (de) 2008-07-23
EP1612221A2 (de) 2006-01-04
WO2001090188A9 (en) 2002-09-19
JP2003533998A (ja) 2003-11-18
AU8178401A (en) 2001-12-03
US20100173408A1 (en) 2010-07-08
US7855270B2 (en) 2010-12-21
CA2407872A1 (en) 2001-11-29
JP4756813B2 (ja) 2011-08-24
DE60112282T2 (de) 2006-04-20
EP1283878B1 (de) 2005-07-27
US20030100021A1 (en) 2003-05-29
AU2001281784B2 (en) 2005-09-15
CA2407872C (en) 2013-11-12
ATE300611T1 (de) 2005-08-15
WO2001090188A3 (en) 2002-06-13

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