DE4139840B4 - Antigen-Zubereitung zum Nachweis von H. pylori - Google Patents
Antigen-Zubereitung zum Nachweis von H. pylori Download PDFInfo
- Publication number
- DE4139840B4 DE4139840B4 DE4139840A DE4139840A DE4139840B4 DE 4139840 B4 DE4139840 B4 DE 4139840B4 DE 4139840 A DE4139840 A DE 4139840A DE 4139840 A DE4139840 A DE 4139840A DE 4139840 B4 DE4139840 B4 DE 4139840B4
- Authority
- DE
- Germany
- Prior art keywords
- pylori
- antigen
- helicobacter pylori
- antigens
- antibodies
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 102000036639 antigens Human genes 0.000 title claims abstract description 80
- 108091007433 antigens Proteins 0.000 title claims abstract description 80
- 239000000427 antigen Substances 0.000 title claims abstract description 79
- 238000002360 preparation method Methods 0.000 title claims description 47
- 238000001514 detection method Methods 0.000 title claims description 15
- 108010046334 Urease Proteins 0.000 claims abstract description 36
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 29
- 238000003556 assay Methods 0.000 claims abstract description 24
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 24
- 230000000694 effects Effects 0.000 claims abstract description 20
- 241000590002 Helicobacter pylori Species 0.000 claims abstract description 19
- 239000000203 mixture Substances 0.000 claims abstract description 18
- 229940037467 helicobacter pylori Drugs 0.000 claims abstract description 17
- 238000004458 analytical method Methods 0.000 claims abstract description 8
- 238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 claims abstract description 8
- 230000009467 reduction Effects 0.000 claims abstract description 3
- 238000004925 denaturation Methods 0.000 claims abstract 2
- 230000036425 denaturation Effects 0.000 claims abstract 2
- 239000000284 extract Substances 0.000 claims description 33
- 238000000034 method Methods 0.000 claims description 20
- 239000012472 biological sample Substances 0.000 claims description 15
- 208000015181 infectious disease Diseases 0.000 claims description 12
- 239000000523 sample Substances 0.000 claims description 10
- 238000002965 ELISA Methods 0.000 claims description 9
- 108090000790 Enzymes Proteins 0.000 claims description 8
- 102000004190 Enzymes Human genes 0.000 claims description 8
- 239000000758 substrate Substances 0.000 claims description 8
- 206010019375 Helicobacter infections Diseases 0.000 claims description 6
- 239000004816 latex Substances 0.000 claims description 6
- 229920000126 latex Polymers 0.000 claims description 6
- 230000008859 change Effects 0.000 claims description 4
- 230000007717 exclusion Effects 0.000 claims description 4
- 238000011161 development Methods 0.000 claims description 3
- 239000007787 solid Substances 0.000 claims description 3
- 108010001336 Horseradish Peroxidase Proteins 0.000 claims description 2
- 238000004440 column chromatography Methods 0.000 claims description 2
- 230000004520 agglutination Effects 0.000 claims 1
- 230000000295 complement effect Effects 0.000 claims 1
- 238000001035 drying Methods 0.000 claims 1
- 238000003127 radioimmunoassay Methods 0.000 claims 1
- 238000001542 size-exclusion chromatography Methods 0.000 claims 1
- 210000004027 cell Anatomy 0.000 description 43
- 239000000243 solution Substances 0.000 description 16
- HEGSGKPQLMEBJL-RKQHYHRCSA-N octyl beta-D-glucopyranoside Chemical compound CCCCCCCCO[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O HEGSGKPQLMEBJL-RKQHYHRCSA-N 0.000 description 15
- 238000012360 testing method Methods 0.000 description 15
- LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 description 13
- 239000012528 membrane Substances 0.000 description 13
- 239000002953 phosphate buffered saline Substances 0.000 description 13
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 12
- PXIPVTKHYLBLMZ-UHFFFAOYSA-N Sodium azide Chemical compound [Na+].[N-]=[N+]=[N-] PXIPVTKHYLBLMZ-UHFFFAOYSA-N 0.000 description 12
- 239000002609 medium Substances 0.000 description 12
- 210000002966 serum Anatomy 0.000 description 10
- 239000003599 detergent Substances 0.000 description 9
- 239000006228 supernatant Substances 0.000 description 9
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 7
- 238000005194 fractionation Methods 0.000 description 7
- 230000035945 sensitivity Effects 0.000 description 7
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 6
- 239000004471 Glycine Substances 0.000 description 6
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 6
- 238000002835 absorbance Methods 0.000 description 6
- 230000000890 antigenic effect Effects 0.000 description 6
- 239000000872 buffer Substances 0.000 description 6
- 238000005119 centrifugation Methods 0.000 description 6
- 230000002496 gastric effect Effects 0.000 description 6
- 239000000499 gel Substances 0.000 description 6
- 229960002449 glycine Drugs 0.000 description 6
- 239000000463 material Substances 0.000 description 6
- 239000004677 Nylon Substances 0.000 description 5
- 238000004519 manufacturing process Methods 0.000 description 5
- 235000015097 nutrients Nutrition 0.000 description 5
- 229920001778 nylon Polymers 0.000 description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 5
- 241000283973 Oryctolagus cuniculus Species 0.000 description 4
- 229920002684 Sepharose Polymers 0.000 description 4
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 4
- 229940098773 bovine serum albumin Drugs 0.000 description 4
- 239000004202 carbamide Substances 0.000 description 4
- 238000003306 harvesting Methods 0.000 description 4
- 238000003018 immunoassay Methods 0.000 description 4
- 210000002381 plasma Anatomy 0.000 description 4
- 230000000405 serological effect Effects 0.000 description 4
- 238000005406 washing Methods 0.000 description 4
- MUBZPKHOEPUJKR-UHFFFAOYSA-N Oxalic acid Chemical compound OC(=O)C(O)=O MUBZPKHOEPUJKR-UHFFFAOYSA-N 0.000 description 3
- 229920001213 Polysorbate 20 Polymers 0.000 description 3
- 238000006243 chemical reaction Methods 0.000 description 3
- 239000008367 deionised water Substances 0.000 description 3
- 229910021641 deionized water Inorganic materials 0.000 description 3
- 238000002270 exclusion chromatography Methods 0.000 description 3
- 238000000605 extraction Methods 0.000 description 3
- 238000011534 incubation Methods 0.000 description 3
- HEGSGKPQLMEBJL-UHFFFAOYSA-N n-octyl beta-D-glucopyranoside Natural products CCCCCCCCOC1OC(CO)C(O)C(O)C1O HEGSGKPQLMEBJL-UHFFFAOYSA-N 0.000 description 3
- 239000008188 pellet Substances 0.000 description 3
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 description 3
- 238000000746 purification Methods 0.000 description 3
- 238000000527 sonication Methods 0.000 description 3
- 239000000725 suspension Substances 0.000 description 3
- PCDWFBFHIIKIPM-UHFFFAOYSA-N 3-ethyl-2h-1,3-benzothiazole-2-sulfonic acid Chemical compound C1=CC=C2N(CC)C(S(O)(=O)=O)SC2=C1 PCDWFBFHIIKIPM-UHFFFAOYSA-N 0.000 description 2
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 2
- 241000589562 Brucella Species 0.000 description 2
- 241000589989 Helicobacter Species 0.000 description 2
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 239000007983 Tris buffer Substances 0.000 description 2
- 239000002250 absorbent Substances 0.000 description 2
- 230000002745 absorbent Effects 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 239000000654 additive Substances 0.000 description 2
- 238000007818 agglutination assay Methods 0.000 description 2
- OHDRQQURAXLVGJ-HLVWOLMTSA-N azane;(2e)-3-ethyl-2-[(e)-(3-ethyl-6-sulfo-1,3-benzothiazol-2-ylidene)hydrazinylidene]-1,3-benzothiazole-6-sulfonic acid Chemical compound [NH4+].[NH4+].S/1C2=CC(S([O-])(=O)=O)=CC=C2N(CC)C\1=N/N=C1/SC2=CC(S([O-])(=O)=O)=CC=C2N1CC OHDRQQURAXLVGJ-HLVWOLMTSA-N 0.000 description 2
- 230000008033 biological extinction Effects 0.000 description 2
- 238000001574 biopsy Methods 0.000 description 2
- 238000009395 breeding Methods 0.000 description 2
- 230000001488 breeding effect Effects 0.000 description 2
- 239000013592 cell lysate Substances 0.000 description 2
- 239000006285 cell suspension Substances 0.000 description 2
- 230000001419 dependent effect Effects 0.000 description 2
- 239000012091 fetal bovine serum Substances 0.000 description 2
- 239000011888 foil Substances 0.000 description 2
- 230000007062 hydrolysis Effects 0.000 description 2
- 238000006460 hydrolysis reaction Methods 0.000 description 2
- 238000007392 microtiter assay Methods 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- 239000012071 phase Substances 0.000 description 2
- 239000004033 plastic Substances 0.000 description 2
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 239000012460 protein solution Substances 0.000 description 2
- 238000009589 serological test Methods 0.000 description 2
- 239000011537 solubilization buffer Substances 0.000 description 2
- 238000001228 spectrum Methods 0.000 description 2
- 210000002784 stomach Anatomy 0.000 description 2
- 208000018556 stomach disease Diseases 0.000 description 2
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 2
- JTNGEYANGCBZLK-UHFFFAOYSA-N 1h-indol-3-yl dihydrogen phosphate Chemical compound C1=CC=C2C(OP(O)(=O)O)=CNC2=C1 JTNGEYANGCBZLK-UHFFFAOYSA-N 0.000 description 1
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 1
- IVLXQGJVBGMLRR-UHFFFAOYSA-N 2-aminoacetic acid;hydron;chloride Chemical compound Cl.NCC(O)=O IVLXQGJVBGMLRR-UHFFFAOYSA-N 0.000 description 1
- HRPVXLWXLXDGHG-UHFFFAOYSA-N Acrylamide Chemical compound NC(=O)C=C HRPVXLWXLXDGHG-UHFFFAOYSA-N 0.000 description 1
- 102000002260 Alkaline Phosphatase Human genes 0.000 description 1
- 108020004774 Alkaline Phosphatase Proteins 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 241000589875 Campylobacter jejuni Species 0.000 description 1
- 208000035473 Communicable disease Diseases 0.000 description 1
- 208000015877 Duodenal disease Diseases 0.000 description 1
- VGGSQFUCUMXWEO-UHFFFAOYSA-N Ethene Chemical compound C=C VGGSQFUCUMXWEO-UHFFFAOYSA-N 0.000 description 1
- 239000005977 Ethylene Substances 0.000 description 1
- 208000012895 Gastric disease Diseases 0.000 description 1
- 208000007882 Gastritis Diseases 0.000 description 1
- 102000018697 Membrane Proteins Human genes 0.000 description 1
- 108010052285 Membrane Proteins Proteins 0.000 description 1
- 239000011837 N,N-methylenebisacrylamide Substances 0.000 description 1
- 208000008469 Peptic Ulcer Diseases 0.000 description 1
- BELBBZDIHDAJOR-UHFFFAOYSA-N Phenolsulfonephthalein Chemical compound C1=CC(O)=CC=C1C1(C=2C=CC(O)=CC=2)C2=CC=CC=C2S(=O)(=O)O1 BELBBZDIHDAJOR-UHFFFAOYSA-N 0.000 description 1
- GOOHAUXETOMSMM-UHFFFAOYSA-N Propylene oxide Chemical compound CC1CO1 GOOHAUXETOMSMM-UHFFFAOYSA-N 0.000 description 1
- 229920000297 Rayon Polymers 0.000 description 1
- 208000025865 Ulcer Diseases 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 150000001298 alcohols Chemical class 0.000 description 1
- 125000001931 aliphatic group Chemical group 0.000 description 1
- 229910021529 ammonia Inorganic materials 0.000 description 1
- 239000012298 atmosphere Substances 0.000 description 1
- 239000011324 bead Substances 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 239000006161 blood agar Substances 0.000 description 1
- UDSAIICHUKSCKT-UHFFFAOYSA-N bromophenol blue Chemical compound C1=C(Br)C(O)=C(Br)C=C1C1(C=2C=C(Br)C(O)=C(Br)C=2)C2=CC=CC=C2S(=O)(=O)O1 UDSAIICHUKSCKT-UHFFFAOYSA-N 0.000 description 1
- 239000006227 byproduct Substances 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 238000011072 cell harvest Methods 0.000 description 1
- 239000007795 chemical reaction product Substances 0.000 description 1
- 239000003593 chromogenic compound Substances 0.000 description 1
- 230000001684 chronic effect Effects 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 230000001332 colony forming effect Effects 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- NKLPQNGYXWVELD-UHFFFAOYSA-M coomassie brilliant blue Chemical compound [Na+].C1=CC(OCC)=CC=C1NC1=CC=C(C(=C2C=CC(C=C2)=[N+](CC)CC=2C=C(C=CC=2)S([O-])(=O)=O)C=2C=CC(=CC=2)N(CC)CC=2C=C(C=CC=2)S([O-])(=O)=O)C=C1 NKLPQNGYXWVELD-UHFFFAOYSA-M 0.000 description 1
- 238000010219 correlation analysis Methods 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 239000002274 desiccant Substances 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 239000012470 diluted sample Substances 0.000 description 1
- 239000003085 diluting agent Substances 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 201000006549 dyspepsia Diseases 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000012894 fetal calf serum Substances 0.000 description 1
- 229960001269 glycine hydrochloride Drugs 0.000 description 1
- 239000001307 helium Substances 0.000 description 1
- 229910052734 helium Inorganic materials 0.000 description 1
- SWQJXJOGLNCZEY-UHFFFAOYSA-N helium atom Chemical compound [He] SWQJXJOGLNCZEY-UHFFFAOYSA-N 0.000 description 1
- 229940098197 human immunoglobulin g Drugs 0.000 description 1
- 230000028993 immune response Effects 0.000 description 1
- 238000003119 immunoblot Methods 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 230000000977 initiatory effect Effects 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- YECIFGHRMFEPJK-UHFFFAOYSA-N lidocaine hydrochloride monohydrate Chemical compound O.[Cl-].CC[NH+](CC)CC(=O)NC1=C(C)C=CC=C1C YECIFGHRMFEPJK-UHFFFAOYSA-N 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000011068 loading method Methods 0.000 description 1
- 230000001926 lymphatic effect Effects 0.000 description 1
- 239000006166 lysate Substances 0.000 description 1
- 230000002906 microbiologic effect Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- ZIUHHBKFKCYYJD-UHFFFAOYSA-N n,n'-methylenebisacrylamide Chemical compound C=CC(=O)NCNC(=O)C=C ZIUHHBKFKCYYJD-UHFFFAOYSA-N 0.000 description 1
- 239000013642 negative control Substances 0.000 description 1
- 239000012299 nitrogen atmosphere Substances 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 235000006408 oxalic acid Nutrition 0.000 description 1
- MPQXHAGKBWFSNV-UHFFFAOYSA-N oxidophosphanium Chemical group [PH3]=O MPQXHAGKBWFSNV-UHFFFAOYSA-N 0.000 description 1
- 239000013610 patient sample Substances 0.000 description 1
- 208000011906 peptic ulcer disease Diseases 0.000 description 1
- 210000005259 peripheral blood Anatomy 0.000 description 1
- 239000011886 peripheral blood Substances 0.000 description 1
- 102000013415 peroxidase activity proteins Human genes 0.000 description 1
- 108040007629 peroxidase activity proteins Proteins 0.000 description 1
- 229960003531 phenolsulfonphthalein Drugs 0.000 description 1
- 238000007747 plating Methods 0.000 description 1
- 238000002264 polyacrylamide gel electrophoresis Methods 0.000 description 1
- 238000011176 pooling Methods 0.000 description 1
- 238000011045 prefiltration Methods 0.000 description 1
- 239000013014 purified material Substances 0.000 description 1
- 206010037844 rash Diseases 0.000 description 1
- 239000002964 rayon Substances 0.000 description 1
- 239000011541 reaction mixture Substances 0.000 description 1
- 239000011347 resin Substances 0.000 description 1
- 229920005989 resin Polymers 0.000 description 1
- 239000012465 retentate Substances 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000012064 sodium phosphate buffer Substances 0.000 description 1
- 239000007790 solid phase Substances 0.000 description 1
- 238000005063 solubilization Methods 0.000 description 1
- 230000007928 solubilization Effects 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 150000003462 sulfoxides Chemical class 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- 150000003512 tertiary amines Chemical class 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- RTKIYNMVFMVABJ-UHFFFAOYSA-L thimerosal Chemical compound [Na+].CC[Hg]SC1=CC=CC=C1C([O-])=O RTKIYNMVFMVABJ-UHFFFAOYSA-L 0.000 description 1
- 229940033663 thimerosal Drugs 0.000 description 1
- 238000001262 western blot Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6854—Immunoglobulins
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/205—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Campylobacter (G)
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56911—Bacteria
- G01N33/56922—Campylobacter
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/195—Assays involving biological materials from specific organisms or of a specific nature from bacteria
- G01N2333/205—Assays involving biological materials from specific organisms or of a specific nature from bacteria from Campylobacter (G)
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/8215—Microorganisms
- Y10S435/822—Microorganisms using bacteria or actinomycetales
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Biochemistry (AREA)
- Organic Chemistry (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Food Science & Technology (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Genetics & Genomics (AREA)
- Biophysics (AREA)
- Gastroenterology & Hepatology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Pharmacology & Pharmacy (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US62184590A | 1990-12-04 | 1990-12-04 | |
| US621845 | 1990-12-04 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DE4139840A1 DE4139840A1 (de) | 1992-06-11 |
| DE4139840B4 true DE4139840B4 (de) | 2005-06-02 |
Family
ID=24491888
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE4139840A Expired - Lifetime DE4139840B4 (de) | 1990-12-04 | 1991-12-03 | Antigen-Zubereitung zum Nachweis von H. pylori |
Country Status (5)
| Country | Link |
|---|---|
| US (2) | US5814455A (cg-RX-API-DMAC7.html) |
| JP (1) | JP3202772B2 (cg-RX-API-DMAC7.html) |
| DE (1) | DE4139840B4 (cg-RX-API-DMAC7.html) |
| FR (1) | FR2669929A1 (cg-RX-API-DMAC7.html) |
| IT (1) | IT1252164B (cg-RX-API-DMAC7.html) |
Families Citing this family (67)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7141244B1 (en) * | 1992-03-02 | 2006-11-28 | Chiron Srl | Helicobacter pylori proteins useful for vaccines and diagnostics |
| MX9301706A (es) * | 1992-04-13 | 1994-05-31 | Oravax Inc | Composicion de vacuna para el tratamiento de la infeccion por helicobacter. |
| US6406703B1 (en) | 1993-07-27 | 2002-06-18 | Csl Limited | Treatment of H. pylori associated gastroduodenal disease |
| ATE244578T1 (de) * | 1993-07-27 | 2003-07-15 | Csl Ltd | Behandlung eines durch helicobakter verursachten gastroduodenalen krankheit |
| DE69529219T2 (de) * | 1994-07-01 | 2003-11-06 | Chiron Corp. (N.D.Ges.D. Staates Delaware), Emeryville | Helicobacter proteine und impstoffe |
| GB9502899D0 (en) * | 1995-02-15 | 1995-04-05 | Cortecs Ltd | Novel antigen |
| FR2736360B1 (fr) | 1995-07-04 | 1997-09-05 | Pasteur Institut | Clonage et caracterisation du gene fiba de h.pylori, production de souches aflagellees |
| FR2739623B1 (fr) * | 1995-10-04 | 1997-12-05 | Pasteur Merieux Serums Vacc | Nouvelle proteine membranaire p76 d'helicobacter pylori |
| FR2739622B1 (fr) * | 1995-10-04 | 1997-12-05 | Pasteur Merieux Serums Vacc | Nouvelles proteines membranaires d'helicobacter pylori |
| DK0909323T3 (da) | 1996-01-04 | 2007-05-21 | Novartis Vaccines & Diagnostic | Helicobacter pylori-bakterioferritin |
| ATE353337T1 (de) * | 1996-06-10 | 2007-02-15 | Thomas Boren | Adhesin aus heliobacter pylori, welches an blutgruppenantigene bindet |
| US6902903B1 (en) * | 1996-12-19 | 2005-06-07 | Chiron Corporation | Helicobacter pylori diagnostics |
| MX9703918A (es) | 1997-05-28 | 1998-11-30 | J Marshall M D Barry | Procedimiento para preparar un producto farmaceutico reactivo para deteccion de desorden gastrointestinal causado por bacteria en el tracto gastrointestinal superior. |
| WO1999053318A1 (en) * | 1998-04-14 | 1999-10-21 | Otsuka Pharmaceutical Co., Ltd. | Methods for assaying antibody and device for assaying antibody |
| WO2000056769A2 (en) * | 1999-03-18 | 2000-09-28 | Cambridge Life Sciences | COMPOSITIONS, TEST KITS AND METHODS FOR DETECTING $i(HELICOBACTER PYLORI) |
| US6682940B2 (en) | 1999-05-04 | 2004-01-27 | Dan A. Pankowsky | Products and methods for single parameter and multiparameter phenotyping of cells |
| US6828157B1 (en) | 1999-05-04 | 2004-12-07 | Dan A. Pankowsky | Products and methods for single parameter and multiparameter phenotyping of cells |
| FR2803302B1 (fr) * | 2000-01-04 | 2004-12-10 | Pf Medicament | Procede de preparation d'un polypeptide soluble en solvant aqueux en absence de detergent |
| US7179609B1 (en) | 2000-10-04 | 2007-02-20 | Atrophus Ab | Screening method for gastritis |
| US20020107368A1 (en) * | 2000-12-07 | 2002-08-08 | Jing-Hui Tian | Helicobacter proteins, gene sequences and uses thereof |
| US20050266499A1 (en) * | 2001-04-25 | 2005-12-01 | Rockeby Biomed Corporation, Ltd. | Method and apparatus for testing for presence or absence of select biological substances |
| RU2196993C1 (ru) * | 2001-06-05 | 2003-01-20 | Санкт-Петербургский научно-исследовательский институт эпидемиологии и микробиологии им. Пастера | Способ серодиагностики helicobacter pylori-инфекции иммуноферментным анализом |
| EP1423142A1 (en) * | 2001-08-31 | 2004-06-02 | Chiron SRL. | Helicobacter pylori vaccination with a combination of caga, vaca and nap proteins |
| USD467348S1 (en) | 2001-10-15 | 2002-12-17 | Kimberly-Clark Worldwide, Inc. | Diagnostic test carrier |
| US6929926B2 (en) | 2001-10-15 | 2005-08-16 | Barry J. Marshall | Composition for the detection of gastrointestinal disorders |
| USD485359S1 (en) | 2001-10-15 | 2004-01-13 | Kimberly-Clark Worldwide, Inc. | Specimen-handling tool |
| US7008777B2 (en) * | 2001-10-15 | 2006-03-07 | Barry J. Marshall | System for the detection of urease and method for using same |
| US6998250B2 (en) | 2001-10-15 | 2006-02-14 | Donald J. McMichael | Method for detecting Helicobacter pylori |
| US6890765B2 (en) * | 2001-11-02 | 2005-05-10 | Roche Diagnostics Operations, Inc. | Particles for immunoassays and methods for treating the same |
| USD469881S1 (en) | 2001-12-17 | 2003-02-04 | Kimberly-Clark Worldwide, Inc. | Two-well diagnostic test kit with specimen-handling tool |
| USD484988S1 (en) | 2001-12-17 | 2004-01-06 | Kimberly-Clark Worldwide, Inc. | Diagnostic test kit with specimen-handling tool |
| USD470241S1 (en) | 2001-12-17 | 2003-02-11 | Kimberly-Clark Worldwide, Inc. | Specimen-handling tool |
| USD470597S1 (en) | 2001-12-17 | 2003-02-18 | Kimberly-Clark Worldwide, Inc. | Diagnostic test kit |
| USD471639S1 (en) | 2001-12-17 | 2003-03-11 | Kimberly-Clark Worldwide, Inc. | Tool for handling a specimen |
| USD470596S1 (en) | 2001-12-17 | 2003-02-18 | Kimberly-Clark Worldwide, Inc. | Test kit specimen-handling tool |
| US6777246B2 (en) | 2001-12-18 | 2004-08-17 | Roche Diagnostics Gmbh | Tertiary amine compounds for use in immunoassays |
| US6783976B2 (en) | 2001-12-21 | 2004-08-31 | Kimberly-Clark Worldwide, Inc. | Carrier and specimen-handling tool for use in diagnostic testing |
| US20030166027A1 (en) * | 2002-02-21 | 2003-09-04 | George Sachs | Compositions, test kits and methods for detecting helicobacter pylori |
| US20050048077A1 (en) * | 2002-02-21 | 2005-03-03 | George Sachs | Compositions, test kits and methods for detecting helicobacter pylori |
| SE0200974D0 (sv) | 2002-03-27 | 2002-03-27 | Phagen Ab | Screening method for gastritis |
| JP4933258B2 (ja) | 2003-09-22 | 2012-05-16 | クイデル コーポレーション | 試料中の複数分析物の検出装置 |
| US7629127B2 (en) * | 2005-01-21 | 2009-12-08 | Dexall Biomedical Labs, Inc. | Method for the visual detection of specific antibodies by the use of lateral flow assays |
| PL3264094T3 (pl) | 2005-04-04 | 2021-04-19 | Biogen Ma Inc. | Sposoby oceniania odpowiedzi immunologicznej na środek leczniczy |
| US7713715B2 (en) * | 2005-09-06 | 2010-05-11 | University Of Tennessee Research Foundation | Method for diagnosing infections |
| EP2676967B1 (en) | 2006-02-28 | 2019-08-14 | Biogen MA Inc. | Methods of treating inflammatory and autoimmune diseases with natalizumab |
| JP5504390B2 (ja) | 2006-03-03 | 2014-05-28 | バイオジェン・アイデック・エムエイ・インコーポレイテッド | ナタリズマブを用いて炎症性疾患及び自己免疫疾患を治療する方法 |
| WO2008021954A2 (en) * | 2006-08-09 | 2008-02-21 | Biogen Idec Ma Inc. | Method for distribution of a drug |
| JP5214723B2 (ja) | 2007-04-30 | 2013-06-19 | ナノゲン・インコーポレイテッド | 多分析物アッセイ |
| CN103254308B (zh) | 2007-06-15 | 2015-01-21 | 厦门大学 | H5亚型禽流感病毒血凝素蛋白的单克隆抗体或其结合活性片段及其用途 |
| US9404911B2 (en) | 2008-04-21 | 2016-08-02 | Quidel Corporation | Integrated assay device and housing |
| US20100322823A1 (en) * | 2008-06-04 | 2010-12-23 | Surapaneni Krishna P | Rapid Detection of Post-Vaccination Antibody Response |
| US8692873B2 (en) | 2009-01-15 | 2014-04-08 | Alverix, Inc. | Video-frame data receiver with low frame capture rate |
| US8422740B2 (en) | 2009-01-15 | 2013-04-16 | Scott Dylewski | Methods for determining a liquid front position on a test strip |
| WO2011044553A1 (en) | 2009-10-11 | 2011-04-14 | Biogen Idec Ma Inc. | Anti-vla-4 related assays |
| SG181653A1 (en) | 2010-01-11 | 2012-07-30 | Biogen Idec Inc | Assay for jc virus antibodies |
| US11287423B2 (en) | 2010-01-11 | 2022-03-29 | Biogen Ma Inc. | Assay for JC virus antibodies |
| US10295472B2 (en) | 2010-05-05 | 2019-05-21 | Alverix, Inc. | Assay reader operable to scan a test strip |
| PT2715352T (pt) | 2011-05-31 | 2019-06-12 | Biogen Ma Inc | Método de avaliação do risco de lmp |
| CN102305857A (zh) * | 2011-08-02 | 2012-01-04 | 深圳市亚辉龙生物科技有限公司 | 一种测定幽门螺杆菌抗体的胶乳免疫试剂及其检测方法 |
| HK1216546A1 (zh) | 2013-05-28 | 2016-11-18 | Biogen Ma Inc. | 评估pml风险的方法 |
| FI20145550A7 (fi) * | 2014-06-13 | 2015-12-14 | Biohit Oyj | Gastriini-17 käyttö biomarkkerina atrofiselle gastriitille ja usealle sen seurauksena kohonneelle riskille |
| FI3785799T3 (fi) | 2015-08-06 | 2023-03-23 | Lia Diagnostics Inc | Veteen dispergoituvien määritysten valmistusmenetelmiä |
| CN106148175A (zh) * | 2016-05-18 | 2016-11-23 | 北京华安麦科生物技术有限公司 | 一种用于富集微生物的方法及其应用 |
| US20200172768A1 (en) | 2018-12-04 | 2020-06-04 | Adhesives Research, Inc. | Disintegrable thin film adhesive barrier |
| FI20205255A1 (en) * | 2020-03-11 | 2021-09-12 | Biohit Oyj | Gastrin-17 test for screening for dyspepsia and reflux symptoms, and atrophic gastritis, and related risks |
| US20250110123A1 (en) * | 2022-01-25 | 2025-04-03 | The Kitasato Institute | Method for detecting helicobacter suis antibody using whole cell |
| JPWO2023145787A1 (cg-RX-API-DMAC7.html) * | 2022-01-25 | 2023-08-03 |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1989008843A1 (en) * | 1988-03-10 | 1989-09-21 | Baylor College Of Medicine | Process for preparation of high molecular weight cell-associated protein of campylobacter pylori and use for serological detection of campylobacter pylori infection |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4981685A (en) * | 1986-03-07 | 1991-01-01 | Utah State University Foundation | Bacterial extract vaccines for veterinary application |
| US5459041A (en) * | 1988-02-18 | 1995-10-17 | Enteric Research Laboratories, Inc. | Campylobacter pylori antigens and uses thereof for detection of Campylobacter pylori infection |
| DE3809796A1 (de) * | 1988-03-23 | 1989-10-05 | Wolfgang Prof Dr Med Bredt | Verfahren zum reinigen eines 168 kd-proteins von mycoplasma pneumoniae |
| WO1989009407A1 (en) * | 1988-03-23 | 1989-10-05 | Julie Claire Dent | Detection of campylobacter pylori urease antibodies and reagent therefor |
| GB2223756A (en) * | 1988-09-29 | 1990-04-18 | Health Lab Service Board | Protein purification process |
-
1991
- 1991-12-03 DE DE4139840A patent/DE4139840B4/de not_active Expired - Lifetime
- 1991-12-04 JP JP32079491A patent/JP3202772B2/ja not_active Expired - Fee Related
- 1991-12-04 IT ITMI913243A patent/IT1252164B/it active IP Right Grant
- 1991-12-04 FR FR9115031A patent/FR2669929A1/fr active Granted
-
1994
- 1994-08-18 US US08/292,932 patent/US5814455A/en not_active Expired - Lifetime
-
1995
- 1995-06-07 US US08/486,843 patent/US5846751A/en not_active Expired - Lifetime
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1989008843A1 (en) * | 1988-03-10 | 1989-09-21 | Baylor College Of Medicine | Process for preparation of high molecular weight cell-associated protein of campylobacter pylori and use for serological detection of campylobacter pylori infection |
Non-Patent Citations (1)
| Title |
|---|
| FEMS Microbiology Letters 71, S.225-230, 1990 * |
Also Published As
| Publication number | Publication date |
|---|---|
| IT1252164B (it) | 1995-06-05 |
| ITMI913243A0 (it) | 1991-12-04 |
| FR2669929B1 (cg-RX-API-DMAC7.html) | 1995-05-05 |
| ITMI913243A1 (it) | 1993-06-04 |
| DE4139840A1 (de) | 1992-06-11 |
| FR2669929A1 (fr) | 1992-06-05 |
| US5814455A (en) | 1998-09-29 |
| JPH05264553A (ja) | 1993-10-12 |
| US5846751A (en) | 1998-12-08 |
| JP3202772B2 (ja) | 2001-08-27 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| DE4139840B4 (de) | Antigen-Zubereitung zum Nachweis von H. pylori | |
| DE68923882T2 (de) | Verfahren zur herstellung des hochmolekulargewichtigen zell-verbundenen proteins von campylobakter pylori und seine verwendung zum serologischen nachweis von campylobakter pylori-infektionen. | |
| DE68928007T2 (de) | Antigene Zusammensetzungen aus Fragmenten von Campylobacter pylori und Verfahren zu ihrer Herstellung und Verwendung | |
| DE3781610T2 (de) | Diagnostikum fuer lyme-krankheit. | |
| DE69709434T3 (de) | Immunoassay für H. pylori in Fäkalienproben | |
| Slots et al. | Detection of Actinobacillus actinomycetemcomitans and Bacteroides gingivalis in subgingival smears by the indirect fluorescent‐antibody technique | |
| DE69934697T2 (de) | Verfahren zur detektion von legionella bakterien unter verwendung aufgereinigter antigen-spezifischer antikörper | |
| DE2836046A1 (de) | Immunologisches bestimmungsverfahren | |
| CH634924A5 (de) | Serologisches verfahren zur bestimmung der gegenwart von neisseria gonorrhoeae-antikoerpern. | |
| DE69725606T2 (de) | Methoden für die diagnose von allergischen atemwegsaspergillosen | |
| DE19649389A1 (de) | Antigenspezifischer IgM-Nachweis | |
| DE68920251T2 (de) | Bestimmung eines chlamydialen oder gonokokkalen Antigens unter Verwendung eines positiv geladenen ionischen Bindungsträgers. | |
| DE69319240T2 (de) | In vitro-test für helicobacter pylori | |
| JP4268358B2 (ja) | 抗体および免疫学的測定方法 | |
| DE69213036T2 (de) | Verfahren zum Nachweis und zur Quantifizierung kariogener Bakterien | |
| DE69910409T2 (de) | Disaccharide-derivate | |
| Tilton | Laboratory aids for the diagnosis of Borrelia burgdorferi infection | |
| EP1278065A1 (en) | Method of detecting streptococcus sobrinus and antibody therefor | |
| DE1598928B2 (de) | Reagens zum nachweis der infektioesen mononukleose | |
| DE3781181T2 (de) | Reagenz mit monoklonalantikoerpern zu bakteroides gingivalis. | |
| Penner et al. | Serotyping of Proteus rettgeri on the basis of O antigens | |
| DE69615537T2 (de) | Helicobacter pylori antigen | |
| Engers | Simplification and standardization of serodiagnostic tests for leprosy based on phenolic glycolipid-I (PG-I) antigen | |
| EP0821237B1 (de) | Immunassay zum Nachweis von anti-B. burgdorferi Antikörpern und Verfahren zur Serodiagnose bei Lyme Borreliose, diagnostische Mittel und Testkits zur Durchführung der Verfahren | |
| USRE34101E (en) | Process for preparation of high molecular weight cell-associated protein of Campylobacter pylori and use for serological detection of Campylobacter pylori infection |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 8110 | Request for examination paragraph 44 | ||
| 8364 | No opposition during term of opposition | ||
| R071 | Expiry of right | ||
| R071 | Expiry of right |