DE4120139A1 - Enzymimmunoassay-verfahren und testset hierzu - Google Patents
Enzymimmunoassay-verfahren und testset hierzuInfo
- Publication number
- DE4120139A1 DE4120139A1 DE19914120139 DE4120139A DE4120139A1 DE 4120139 A1 DE4120139 A1 DE 4120139A1 DE 19914120139 DE19914120139 DE 19914120139 DE 4120139 A DE4120139 A DE 4120139A DE 4120139 A1 DE4120139 A1 DE 4120139A1
- Authority
- DE
- Germany
- Prior art keywords
- plate
- enzyme
- antibody
- antigen
- pins
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 230000000295 complement effect Effects 0.000 title claims abstract description 35
- 238000003018 immunoassay Methods 0.000 title claims abstract description 10
- 239000000758 substrate Substances 0.000 title claims description 12
- 238000000034 method Methods 0.000 title description 7
- 239000000427 antigen Substances 0.000 claims abstract description 33
- 108091007433 antigens Proteins 0.000 claims abstract description 29
- 102000036639 antigens Human genes 0.000 claims abstract description 29
- 238000005406 washing Methods 0.000 claims abstract description 12
- 238000005259 measurement Methods 0.000 claims abstract 2
- 108090000790 Enzymes Proteins 0.000 claims description 25
- 102000004190 Enzymes Human genes 0.000 claims description 25
- 238000012360 testing method Methods 0.000 claims description 13
- 230000000694 effects Effects 0.000 claims description 5
- 239000007790 solid phase Substances 0.000 claims description 4
- 238000002845 discoloration Methods 0.000 claims description 3
- 239000003153 chemical reaction reagent Substances 0.000 claims 2
- 108090000623 proteins and genes Proteins 0.000 claims 1
- 238000011534 incubation Methods 0.000 abstract description 19
- 239000000376 reactant Substances 0.000 abstract description 6
- 238000009792 diffusion process Methods 0.000 abstract description 3
- 230000003247 decreasing effect Effects 0.000 abstract description 2
- 238000003556 assay Methods 0.000 abstract 1
- 239000012530 fluid Substances 0.000 abstract 1
- 238000007654 immersion Methods 0.000 abstract 1
- 210000002966 serum Anatomy 0.000 description 5
- 238000002965 ELISA Methods 0.000 description 4
- 241000590002 Helicobacter pylori Species 0.000 description 3
- 238000011156 evaluation Methods 0.000 description 3
- 229940037467 helicobacter pylori Drugs 0.000 description 3
- 238000009666 routine test Methods 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 208000007882 Gastritis Diseases 0.000 description 1
- 241000162682 Heterogen Species 0.000 description 1
- 208000007107 Stomach Ulcer Diseases 0.000 description 1
- 210000000080 chela (arthropods) Anatomy 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000007598 dipping method Methods 0.000 description 1
- 208000000718 duodenal ulcer Diseases 0.000 description 1
- 230000002496 gastric effect Effects 0.000 description 1
- 238000009790 rate-determining step (RDS) Methods 0.000 description 1
- 230000035484 reaction time Effects 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000009736 wetting Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/5302—Apparatus specially adapted for immunological test procedures
- G01N33/5304—Reaction vessels, e.g. agglutination plates
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54366—Apparatus specially adapted for solid-phase testing
Landscapes
- Health & Medical Sciences (AREA)
- Immunology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE19914120139 DE4120139A1 (de) | 1991-06-19 | 1991-06-19 | Enzymimmunoassay-verfahren und testset hierzu |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE19914120139 DE4120139A1 (de) | 1991-06-19 | 1991-06-19 | Enzymimmunoassay-verfahren und testset hierzu |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DE4120139A1 true DE4120139A1 (de) | 1992-12-24 |
| DE4120139C2 DE4120139C2 (enrdf_load_stackoverflow) | 1993-09-23 |
Family
ID=6434240
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE19914120139 Granted DE4120139A1 (de) | 1991-06-19 | 1991-06-19 | Enzymimmunoassay-verfahren und testset hierzu |
Country Status (1)
| Country | Link |
|---|---|
| DE (1) | DE4120139A1 (enrdf_load_stackoverflow) |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE4339795A1 (de) * | 1993-11-17 | 1995-05-18 | Hunger Hans Dieter Dr | Verfahren zum Nachweis von Biomolekülen |
| EP0709678A1 (en) * | 1994-10-25 | 1996-05-01 | Sumitomo Pharmaceuticals Company, Limited | Immunoassay plate |
| WO2003085401A1 (en) * | 2002-04-11 | 2003-10-16 | Moliseinnovazione Soc. Cons. A R.L. | Device and method to simultaneously detect different antibodies and antigens in clinical alimentary and environmental samples |
| EP1355146A3 (en) * | 2002-04-15 | 2003-12-17 | Becton Dickinson and Company | Use of the multipin platform as anchor device |
| WO2003095662A3 (en) * | 2002-05-08 | 2004-03-11 | Novartis Ag | Simultaneous detection of two analytes produced by a cell under the influence of a putative agent using a pin coated assay system |
| ITCS20110012A1 (it) * | 2011-04-21 | 2012-10-22 | Uni Degli Studi Del Molise | Metodo analitico di competizione tra 2 fasi solide per il rilevamento simultaneo di differenti marker cellulari o molecolari, dispositivo costituito da micropiastre o microstrip con pozzetti di forma allungata per l'esecuzione di detto metodo e relat |
| WO2012143912A1 (en) * | 2011-04-21 | 2012-10-26 | Università Degli Studi Del Molise | Device, method and kit for the detection of different markers in different cellular or molecular types and their quantification |
| EP3368909A4 (en) * | 2015-10-29 | 2019-07-03 | Vibrant Holdings, LLC | METHODS, TOOLS AND TOOL ASSEMBLY FOR BIOMOLECULAR ANALYSIS USING MICROARRAYS |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE29803626U1 (de) * | 1998-03-03 | 1998-05-07 | Pache, Wolfgang, 79294 Sölden | Pinplatte |
| DE10227962B4 (de) * | 2002-06-22 | 2005-12-15 | Lavision Biotec Gmbh | Grundkörper für einen Bio-Chip, Anordnung zum Auslesen und Vorrichtung zur Hybridisierung |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE9100320U1 (de) * | 1991-01-09 | 1991-04-25 | Behm, Erasmus, Dr.sc.med., O-2540 Rostock | Vorrichtung zur Durchführung eines Immunoassays |
-
1991
- 1991-06-19 DE DE19914120139 patent/DE4120139A1/de active Granted
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE9100320U1 (de) * | 1991-01-09 | 1991-04-25 | Behm, Erasmus, Dr.sc.med., O-2540 Rostock | Vorrichtung zur Durchführung eines Immunoassays |
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE4339795A1 (de) * | 1993-11-17 | 1995-05-18 | Hunger Hans Dieter Dr | Verfahren zum Nachweis von Biomolekülen |
| EP0709678A1 (en) * | 1994-10-25 | 1996-05-01 | Sumitomo Pharmaceuticals Company, Limited | Immunoassay plate |
| WO2003085401A1 (en) * | 2002-04-11 | 2003-10-16 | Moliseinnovazione Soc. Cons. A R.L. | Device and method to simultaneously detect different antibodies and antigens in clinical alimentary and environmental samples |
| US7510687B2 (en) | 2002-04-11 | 2009-03-31 | Alessandra Mazzeo | Simultaneous detection of different antibodies and antigens in clinical alimentary and environmental samples |
| EP1355146A3 (en) * | 2002-04-15 | 2003-12-17 | Becton Dickinson and Company | Use of the multipin platform as anchor device |
| WO2003095662A3 (en) * | 2002-05-08 | 2004-03-11 | Novartis Ag | Simultaneous detection of two analytes produced by a cell under the influence of a putative agent using a pin coated assay system |
| ITCS20110012A1 (it) * | 2011-04-21 | 2012-10-22 | Uni Degli Studi Del Molise | Metodo analitico di competizione tra 2 fasi solide per il rilevamento simultaneo di differenti marker cellulari o molecolari, dispositivo costituito da micropiastre o microstrip con pozzetti di forma allungata per l'esecuzione di detto metodo e relat |
| WO2012143912A1 (en) * | 2011-04-21 | 2012-10-26 | Università Degli Studi Del Molise | Device, method and kit for the detection of different markers in different cellular or molecular types and their quantification |
| EP3368909A4 (en) * | 2015-10-29 | 2019-07-03 | Vibrant Holdings, LLC | METHODS, TOOLS AND TOOL ASSEMBLY FOR BIOMOLECULAR ANALYSIS USING MICROARRAYS |
| US11033904B2 (en) | 2015-10-29 | 2021-06-15 | Vibrant Holdings, Llc | Methods, tools, and tool assemblies for biomolecular analysis using microarrays |
Also Published As
| Publication number | Publication date |
|---|---|
| DE4120139C2 (enrdf_load_stackoverflow) | 1993-09-23 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| OP8 | Request for examination as to paragraph 44 patent law | ||
| D2 | Grant after examination | ||
| 8364 | No opposition during term of opposition | ||
| 8339 | Ceased/non-payment of the annual fee |