DE4001518C2 - - Google Patents
Info
- Publication number
- DE4001518C2 DE4001518C2 DE4001518A DE4001518A DE4001518C2 DE 4001518 C2 DE4001518 C2 DE 4001518C2 DE 4001518 A DE4001518 A DE 4001518A DE 4001518 A DE4001518 A DE 4001518A DE 4001518 C2 DE4001518 C2 DE 4001518C2
- Authority
- DE
- Germany
- Prior art keywords
- fed
- batch section
- growth rate
- glucose
- oxygen
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Revoked
Links
- 238000000034 method Methods 0.000 claims abstract description 51
- 241000588724 Escherichia coli Species 0.000 claims abstract description 39
- 238000000855 fermentation Methods 0.000 claims abstract description 31
- 230000004151 fermentation Effects 0.000 claims abstract description 31
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 52
- 230000012010 growth Effects 0.000 claims description 52
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 42
- 239000008103 glucose Substances 0.000 claims description 42
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 claims description 35
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 34
- 239000001301 oxygen Substances 0.000 claims description 34
- 229910052760 oxygen Inorganic materials 0.000 claims description 34
- 239000000243 solution Substances 0.000 claims description 34
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 26
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 26
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims description 24
- QGZKDVFQNNGYKY-UHFFFAOYSA-N ammonia Natural products N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 claims description 22
- 235000015097 nutrients Nutrition 0.000 claims description 19
- 150000003839 salts Chemical class 0.000 claims description 15
- 239000002518 antifoaming agent Substances 0.000 claims description 11
- 239000002028 Biomass Substances 0.000 claims description 10
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 10
- ZGTMUACCHSMWAC-UHFFFAOYSA-L EDTA disodium salt (anhydrous) Chemical compound [Na+].[Na+].OC(=O)CN(CC([O-])=O)CCN(CC(O)=O)CC([O-])=O ZGTMUACCHSMWAC-UHFFFAOYSA-L 0.000 claims description 10
- 229910052799 carbon Inorganic materials 0.000 claims description 10
- 229910052500 inorganic mineral Inorganic materials 0.000 claims description 9
- NPFOYSMITVOQOS-UHFFFAOYSA-K iron(III) citrate Chemical compound [Fe+3].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NPFOYSMITVOQOS-UHFFFAOYSA-K 0.000 claims description 9
- 235000010755 mineral Nutrition 0.000 claims description 9
- 239000011707 mineral Substances 0.000 claims description 9
- 229910021380 Manganese Chloride Inorganic materials 0.000 claims description 8
- GLFNIEUTAYBVOC-UHFFFAOYSA-L Manganese chloride Chemical compound Cl[Mn]Cl GLFNIEUTAYBVOC-UHFFFAOYSA-L 0.000 claims description 8
- KGBXLFKZBHKPEV-UHFFFAOYSA-N boric acid Chemical compound OB(O)O KGBXLFKZBHKPEV-UHFFFAOYSA-N 0.000 claims description 8
- ORTQZVOHEJQUHG-UHFFFAOYSA-L copper(II) chloride Chemical compound Cl[Cu]Cl ORTQZVOHEJQUHG-UHFFFAOYSA-L 0.000 claims description 8
- 239000011565 manganese chloride Substances 0.000 claims description 8
- 235000002867 manganese chloride Nutrition 0.000 claims description 8
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 8
- 235000019796 monopotassium phosphate Nutrition 0.000 claims description 8
- 239000011684 sodium molybdate Substances 0.000 claims description 8
- 235000015393 sodium molybdate Nutrition 0.000 claims description 8
- TVXXNOYZHKPKGW-UHFFFAOYSA-N sodium molybdate (anhydrous) Chemical compound [Na+].[Na+].[O-][Mo]([O-])(=O)=O TVXXNOYZHKPKGW-UHFFFAOYSA-N 0.000 claims description 8
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims description 7
- JZRWCGZRTZMZEH-UHFFFAOYSA-N Thiamine Natural products CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N JZRWCGZRTZMZEH-UHFFFAOYSA-N 0.000 claims description 7
- 239000000758 substrate Substances 0.000 claims description 7
- KYMBYSLLVAOCFI-UHFFFAOYSA-N thiamine Chemical compound CC1=C(CCO)SCN1CC1=CN=C(C)N=C1N KYMBYSLLVAOCFI-UHFFFAOYSA-N 0.000 claims description 7
- 229960003495 thiamine Drugs 0.000 claims description 7
- 235000019157 thiamine Nutrition 0.000 claims description 7
- 239000011721 thiamine Substances 0.000 claims description 7
- 238000006213 oxygenation reaction Methods 0.000 claims description 6
- NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonium chloride Substances [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 claims description 5
- 235000011114 ammonium hydroxide Nutrition 0.000 claims description 5
- VHUUQVKOLVNVRT-UHFFFAOYSA-N Ammonium hydroxide Chemical compound [NH4+].[OH-] VHUUQVKOLVNVRT-UHFFFAOYSA-N 0.000 claims description 4
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 4
- 229910021580 Cobalt(II) chloride Inorganic materials 0.000 claims description 4
- 229910021592 Copper(II) chloride Inorganic materials 0.000 claims description 4
- MYMOFIZGZYHOMD-UHFFFAOYSA-N Dioxygen Chemical compound O=O MYMOFIZGZYHOMD-UHFFFAOYSA-N 0.000 claims description 4
- 239000007836 KH2PO4 Substances 0.000 claims description 4
- 229910004619 Na2MoO4 Inorganic materials 0.000 claims description 4
- ZOIORXHNWRGPMV-UHFFFAOYSA-N acetic acid;zinc Chemical compound [Zn].CC(O)=O.CC(O)=O ZOIORXHNWRGPMV-UHFFFAOYSA-N 0.000 claims description 4
- 230000033228 biological regulation Effects 0.000 claims description 4
- 239000004327 boric acid Substances 0.000 claims description 4
- GVPFVAHMJGGAJG-UHFFFAOYSA-L cobalt dichloride Chemical compound [Cl-].[Cl-].[Co+2] GVPFVAHMJGGAJG-UHFFFAOYSA-L 0.000 claims description 4
- MNNHAPBLZZVQHP-UHFFFAOYSA-N diammonium hydrogen phosphate Chemical compound [NH4+].[NH4+].OP([O-])([O-])=O MNNHAPBLZZVQHP-UHFFFAOYSA-N 0.000 claims description 4
- 235000019838 diammonium phosphate Nutrition 0.000 claims description 4
- 229910000388 diammonium phosphate Inorganic materials 0.000 claims description 4
- 229940099607 manganese chloride Drugs 0.000 claims description 4
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 claims description 4
- 239000011550 stock solution Substances 0.000 claims description 4
- 239000011701 zinc Substances 0.000 claims description 4
- 239000004246 zinc acetate Substances 0.000 claims description 4
- 235000011187 glycerol Nutrition 0.000 claims description 3
- GUBGYTABKSRVRQ-XLOQQCSPSA-N Alpha-Lactose Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)O[C@H](O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-XLOQQCSPSA-N 0.000 claims description 2
- 241001646716 Escherichia coli K-12 Species 0.000 claims description 2
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 claims description 2
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 2
- 229930006000 Sucrose Natural products 0.000 claims description 2
- 230000003247 decreasing effect Effects 0.000 claims description 2
- 150000002016 disaccharides Chemical class 0.000 claims description 2
- 235000001727 glucose Nutrition 0.000 claims description 2
- 239000008101 lactose Substances 0.000 claims description 2
- 150000002772 monosaccharides Chemical group 0.000 claims description 2
- 229910052757 nitrogen Inorganic materials 0.000 claims description 2
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 claims description 2
- 229920005862 polyol Polymers 0.000 claims description 2
- 150000003077 polyols Chemical class 0.000 claims description 2
- 230000001105 regulatory effect Effects 0.000 claims description 2
- 230000001954 sterilising effect Effects 0.000 claims description 2
- 239000005720 sucrose Substances 0.000 claims description 2
- 238000004659 sterilization and disinfection Methods 0.000 claims 1
- 229960001031 glucose Drugs 0.000 description 33
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 26
- 229910001868 water Inorganic materials 0.000 description 25
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 8
- 238000007792 addition Methods 0.000 description 6
- 229910021529 ammonia Inorganic materials 0.000 description 6
- 238000003958 fumigation Methods 0.000 description 6
- 230000004060 metabolic process Effects 0.000 description 5
- 239000000047 product Substances 0.000 description 5
- 230000015572 biosynthetic process Effects 0.000 description 4
- 229910002092 carbon dioxide Inorganic materials 0.000 description 4
- 238000011081 inoculation Methods 0.000 description 4
- 108020004511 Recombinant DNA Proteins 0.000 description 3
- 238000011021 bench scale process Methods 0.000 description 3
- 239000006227 byproduct Substances 0.000 description 3
- 229940041514 candida albicans extract Drugs 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 230000002401 inhibitory effect Effects 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 238000005259 measurement Methods 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 239000012138 yeast extract Substances 0.000 description 3
- 241001522878 Escherichia coli B Species 0.000 description 2
- 239000001569 carbon dioxide Substances 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 239000011573 trace mineral Substances 0.000 description 2
- 235000013619 trace mineral Nutrition 0.000 description 2
- 239000013598 vector Substances 0.000 description 2
- 241000251468 Actinopterygii Species 0.000 description 1
- 102000002723 Atrial Natriuretic Factor Human genes 0.000 description 1
- 101800001288 Atrial natriuretic factor Proteins 0.000 description 1
- 101800001890 Atrial natriuretic peptide Proteins 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 241000901842 Escherichia coli W Species 0.000 description 1
- 101000976075 Homo sapiens Insulin Proteins 0.000 description 1
- 101001033249 Homo sapiens Interleukin-1 beta Proteins 0.000 description 1
- 102000014150 Interferons Human genes 0.000 description 1
- 108010050904 Interferons Proteins 0.000 description 1
- 208000012868 Overgrowth Diseases 0.000 description 1
- 239000001888 Peptone Substances 0.000 description 1
- 108010080698 Peptones Proteins 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 102000013275 Somatomedins Human genes 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 241001148470 aerobic bacillus Species 0.000 description 1
- 235000005550 amino acid supplement Nutrition 0.000 description 1
- 230000003698 anagen phase Effects 0.000 description 1
- 238000010923 batch production Methods 0.000 description 1
- 230000006696 biosynthetic metabolic pathway Effects 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- NSQLIUXCMFBZME-MPVJKSABSA-N carperitide Chemical compound C([C@H]1C(=O)NCC(=O)NCC(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@H](C(NCC(=O)N[C@@H](C)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CO)C(=O)NCC(=O)N[C@@H](CC(C)C)C(=O)NCC(=O)N[C@@H](CSSC[C@@H](C(=O)N1)NC(=O)[C@H](CO)NC(=O)[C@H](CO)NC(=O)[C@H](CCCNC(N)=N)NC(=O)[C@H](CCCNC(N)=N)NC(=O)[C@H](CC(C)C)NC(=O)[C@@H](N)CO)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CO)C(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(O)=O)=O)[C@@H](C)CC)C1=CC=CC=C1 NSQLIUXCMFBZME-MPVJKSABSA-N 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 239000008367 deionised water Substances 0.000 description 1
- 229910021641 deionized water Inorganic materials 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 230000000368 destabilizing effect Effects 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 235000020774 essential nutrients Nutrition 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 230000009036 growth inhibition Effects 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- PBGKTOXHQIOBKM-FHFVDXKLSA-N insulin (human) Chemical compound C([C@@H](C(=O)N[C@@H](CC(C)C)C(=O)N[C@H]1CSSC[C@H]2C(=O)N[C@H](C(=O)N[C@@H](CO)C(=O)N[C@H](C(=O)N[C@H](C(N[C@@H](CO)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC=3C=CC(O)=CC=3)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CC=3C=CC(O)=CC=3)C(=O)N[C@@H](CSSC[C@H](NC(=O)[C@H](C(C)C)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC=3C=CC(O)=CC=3)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](C)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](C(C)C)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC=3NC=NC=3)NC(=O)[C@H](CO)NC(=O)CNC1=O)C(=O)NCC(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)NCC(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(=O)N[C@@H]([C@@H](C)O)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H]([C@@H](C)O)C(O)=O)C(=O)N[C@@H](CC(N)=O)C(O)=O)=O)CSSC[C@@H](C(N2)=O)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](C(C)C)NC(=O)[C@@H](NC(=O)CN)[C@@H](C)CC)[C@@H](C)CC)[C@@H](C)O)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](CC(N)=O)NC(=O)[C@@H](NC(=O)[C@@H](N)CC=1C=CC=CC=1)C(C)C)C1=CN=CN1 PBGKTOXHQIOBKM-FHFVDXKLSA-N 0.000 description 1
- 229940079322 interferon Drugs 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 239000012092 media component Substances 0.000 description 1
- 230000004066 metabolic change Effects 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 229910000069 nitrogen hydride Inorganic materials 0.000 description 1
- 239000005416 organic matter Substances 0.000 description 1
- 125000001477 organic nitrogen group Chemical group 0.000 description 1
- 230000036284 oxygen consumption Effects 0.000 description 1
- 235000019319 peptone Nutrition 0.000 description 1
- 238000011020 pilot scale process Methods 0.000 description 1
- 239000013612 plasmid Substances 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000003259 recombinant expression Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 235000002639 sodium chloride Nutrition 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 230000009469 supplementation Effects 0.000 description 1
- 230000001502 supplementing effect Effects 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Virology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Tropical Medicine & Parasitology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Priority Applications (10)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE4001518A DE4001518A1 (de) | 1990-01-19 | 1990-01-19 | Verfahren zur hochzelldichte-fermentation von escherichia coli in einem ruehrkesselfermentor |
| PCT/EP1990/002301 WO1991010721A2 (de) | 1990-01-19 | 1990-12-24 | Verfahren zur hochzelldichte-fermentation von escherichia coli in einem rührkesselfermentor |
| EP91901288A EP0511226B1 (de) | 1990-01-19 | 1990-12-24 | Verfahren zur hochzelldichte-fermentation von escherichia coli in einem rührkesselfermentor |
| DE59008683T DE59008683D1 (de) | 1990-01-19 | 1990-12-24 | Verfahren zur hochzelldichte-fermentation von escherichia coli in einem rührkesselfermentor. |
| AT91901288T ATE119570T1 (de) | 1990-01-19 | 1990-12-24 | Verfahren zur hochzelldichte-fermentation von escherichia coli in einem rührkesselfermentor. |
| CA002073974A CA2073974A1 (en) | 1990-01-19 | 1990-12-24 | Process for the high density fermentation of escherichia coli in an agitator vessel fermentor |
| DK91901288.0T DK0511226T3 (da) | 1990-01-19 | 1990-12-24 | Fremgangsmåde til fermentering med høj celletæthed af Escherichia coli i en rørefermenteringsbeholder |
| BR909007980A BR9007980A (pt) | 1990-01-19 | 1990-12-24 | Processo para a fermentacao com elevada densidade celular de escherichia coli em um fermentador de tanque agitado |
| HU9202332A HUT65613A (en) | 1990-01-19 | 1990-12-24 | Process for high cell density fermentation of escherichia coli in an agitated boiler fermenter |
| CS91117A CS11791A2 (en) | 1990-01-19 | 1991-01-18 | Method of fermentation with high cellular density of escherichia coli bacteria in stirring fermentation kettle |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE4001518A DE4001518A1 (de) | 1990-01-19 | 1990-01-19 | Verfahren zur hochzelldichte-fermentation von escherichia coli in einem ruehrkesselfermentor |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DE4001518A1 DE4001518A1 (de) | 1991-07-25 |
| DE4001518C2 true DE4001518C2 (enExample) | 1992-01-30 |
Family
ID=6398409
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE4001518A Granted DE4001518A1 (de) | 1990-01-19 | 1990-01-19 | Verfahren zur hochzelldichte-fermentation von escherichia coli in einem ruehrkesselfermentor |
| DE59008683T Expired - Lifetime DE59008683D1 (de) | 1990-01-19 | 1990-12-24 | Verfahren zur hochzelldichte-fermentation von escherichia coli in einem rührkesselfermentor. |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE59008683T Expired - Lifetime DE59008683D1 (de) | 1990-01-19 | 1990-12-24 | Verfahren zur hochzelldichte-fermentation von escherichia coli in einem rührkesselfermentor. |
Country Status (9)
| Country | Link |
|---|---|
| EP (1) | EP0511226B1 (enExample) |
| AT (1) | ATE119570T1 (enExample) |
| BR (1) | BR9007980A (enExample) |
| CA (1) | CA2073974A1 (enExample) |
| CS (1) | CS11791A2 (enExample) |
| DE (2) | DE4001518A1 (enExample) |
| DK (1) | DK0511226T3 (enExample) |
| HU (1) | HUT65613A (enExample) |
| WO (1) | WO1991010721A2 (enExample) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5981735A (en) | 1996-02-12 | 1999-11-09 | Cobra Therapeutics Limited | Method of plasmid DNA production and purification |
| GB9602825D0 (en) * | 1996-02-12 | 1996-04-10 | Therexsys Ltd | Method of plasmid dna production and purification |
| RU2631001C2 (ru) | 2010-08-30 | 2017-09-15 | Ф.Хоффманн-Ля Рош Аг | Способ получения полипептида |
| KR102036671B1 (ko) | 2012-03-12 | 2019-11-26 | 한미사이언스 주식회사 | 대장균의 고농도 배양방법 |
| CN114127256A (zh) | 2019-02-20 | 2022-03-01 | 巴斯夫欧洲公司 | 用确定成分培养基和微量元素补料的芽孢杆菌工业发酵工艺 |
| US20220186177A1 (en) | 2019-02-20 | 2022-06-16 | Basf Se | Industrial fermentation process for bacillus using defined medium and magnesium feed |
| CN115786214B (zh) * | 2022-12-26 | 2023-08-18 | 湖北擎科生物科技有限公司 | 感受态大肠杆菌的高密度发酵培养基及高密度发酵培养方法 |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE2601254B2 (de) * | 1976-01-15 | 1977-11-10 | B. Braun Melsungen Ag, 3508 Melsungen | Verfahren zur regelung des partialdruckes von gasen in fermentoren |
| NZ183731A (en) * | 1976-04-02 | 1980-04-28 | Ici Ltd | Process for culturing cells(microorganism and tissue culture) dependent on the relationship between cycle time and biomass efficiency ratio |
| FR2505359B1 (fr) * | 1981-05-08 | 1985-07-05 | Air Liquide | Procede et installation de fabrication de microorganismes |
| CA1293217C (en) * | 1987-11-09 | 1991-12-17 | Sooyoung Stanford Lee | Controlled growth rate fermentation |
| EP0345588A1 (de) * | 1988-06-03 | 1989-12-13 | Gesellschaft für Biotechnologische Forschung mbH (GBF) | Verfahren und Anordnung zur Regelung eines biologischen Wachstumsprozesses |
-
1990
- 1990-01-19 DE DE4001518A patent/DE4001518A1/de active Granted
- 1990-12-24 DE DE59008683T patent/DE59008683D1/de not_active Expired - Lifetime
- 1990-12-24 BR BR909007980A patent/BR9007980A/pt unknown
- 1990-12-24 WO PCT/EP1990/002301 patent/WO1991010721A2/de not_active Ceased
- 1990-12-24 CA CA002073974A patent/CA2073974A1/en not_active Abandoned
- 1990-12-24 EP EP91901288A patent/EP0511226B1/de not_active Expired - Lifetime
- 1990-12-24 DK DK91901288.0T patent/DK0511226T3/da active
- 1990-12-24 AT AT91901288T patent/ATE119570T1/de not_active IP Right Cessation
- 1990-12-24 HU HU9202332A patent/HUT65613A/hu unknown
-
1991
- 1991-01-18 CS CS91117A patent/CS11791A2/cs unknown
Also Published As
| Publication number | Publication date |
|---|---|
| DK0511226T3 (da) | 1995-04-10 |
| WO1991010721A2 (de) | 1991-07-25 |
| CA2073974A1 (en) | 1991-07-20 |
| DE4001518A1 (de) | 1991-07-25 |
| EP0511226A1 (de) | 1992-11-04 |
| HUT65613A (en) | 1994-07-28 |
| CS11791A2 (en) | 1991-08-13 |
| EP0511226B1 (de) | 1995-03-08 |
| HU9202332D0 (en) | 1993-05-28 |
| BR9007980A (pt) | 1992-11-17 |
| WO1991010721A3 (de) | 1991-09-19 |
| DE59008683D1 (de) | 1995-04-13 |
| ATE119570T1 (de) | 1995-03-15 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| OP8 | Request for examination as to paragraph 44 patent law | ||
| D2 | Grant after examination | ||
| 8363 | Opposition against the patent | ||
| 8331 | Complete revocation |