DE3853060D1 - Verfahren zur Herstellung von Oligonucleotiden. - Google Patents
Verfahren zur Herstellung von Oligonucleotiden.Info
- Publication number
- DE3853060D1 DE3853060D1 DE3853060T DE3853060T DE3853060D1 DE 3853060 D1 DE3853060 D1 DE 3853060D1 DE 3853060 T DE3853060 T DE 3853060T DE 3853060 T DE3853060 T DE 3853060T DE 3853060 D1 DE3853060 D1 DE 3853060D1
- Authority
- DE
- Germany
- Prior art keywords
- oligonucleotides
- preparation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 108091034117 Oligonucleotide Proteins 0.000 title 1
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 title 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
- C07F9/00—Compounds containing elements of Groups 5 or 15 of the Periodic Table
- C07F9/02—Phosphorus compounds
- C07F9/06—Phosphorus compounds without P—C bonds
- C07F9/22—Amides of acids of phosphorus
- C07F9/24—Esteramides
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Genetics & Genomics (AREA)
- Saccharide Compounds (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US07/138,287 US4965349A (en) | 1987-12-24 | 1987-12-24 | Method of synthesizing oligonucleotides labeled with ammonia-labile groups on solid phase supports |
Publications (2)
Publication Number | Publication Date |
---|---|
DE3853060D1 true DE3853060D1 (de) | 1995-03-23 |
DE3853060T2 DE3853060T2 (de) | 1995-10-19 |
Family
ID=22481345
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DE3853060T Expired - Fee Related DE3853060T2 (de) | 1987-12-24 | 1988-12-22 | Verfahren zur Herstellung von Oligonucleotiden. |
DE3856481T Expired - Fee Related DE3856481T2 (de) | 1987-12-24 | 1988-12-22 | Farbstoffmarkierte Phosphoramaditen |
Family Applications After (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DE3856481T Expired - Fee Related DE3856481T2 (de) | 1987-12-24 | 1988-12-22 | Farbstoffmarkierte Phosphoramaditen |
Country Status (4)
Country | Link |
---|---|
US (1) | US4965349A (de) |
EP (2) | EP0617047B1 (de) |
JP (1) | JP2787775B2 (de) |
DE (2) | DE3853060T2 (de) |
Families Citing this family (53)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5231191A (en) * | 1987-12-24 | 1993-07-27 | Applied Biosystems, Inc. | Rhodamine phosphoramidite compounds |
US4997928A (en) * | 1988-09-15 | 1991-03-05 | E. I. Du Pont De Nemours And Company | Fluorescent reagents for the preparation of 5'-tagged oligonucleotides |
US5262536A (en) * | 1988-09-15 | 1993-11-16 | E. I. Du Pont De Nemours And Company | Reagents for the preparation of 5'-tagged oligonucleotides |
US5366860A (en) * | 1989-09-29 | 1994-11-22 | Applied Biosystems, Inc. | Spectrally resolvable rhodamine dyes for nucleic acid sequence determination |
US5321131A (en) * | 1990-03-08 | 1994-06-14 | Hybridon, Inc. | Site-specific functionalization of oligodeoxynucleotides for non-radioactive labelling |
US5316908A (en) * | 1990-07-13 | 1994-05-31 | Life Technologies, Inc. | Size markers for electrophoretic analysis of DNA |
US5371241A (en) * | 1991-07-19 | 1994-12-06 | Pharmacia P-L Biochemicals Inc. | Fluorescein labelled phosphoramidites |
US5573905A (en) * | 1992-03-30 | 1996-11-12 | The Scripps Research Institute | Encoded combinatorial chemical libraries |
US5616700A (en) * | 1992-04-24 | 1997-04-01 | Beckman Instruments, Inc. | Processes for synthesizing nucleotides and modified nucleotides using N.sub. |
US5348868A (en) * | 1992-04-24 | 1994-09-20 | Beckman Instruments, Inc. | Methods and reagents for cleaving and deprotecting oligonucleotides |
US5428148A (en) * | 1992-04-24 | 1995-06-27 | Beckman Instruments, Inc. | N4 - acylated cytidinyl compounds useful in oligonucleotide synthesis |
US5627277A (en) * | 1992-12-16 | 1997-05-06 | Hybridon, Inc. | Method for analyzing oligonucleotide analogs |
US5643717A (en) * | 1992-12-16 | 1997-07-01 | Hybridon, Inc. | Substrate useful for separating modified oligonucleotides |
US5506103A (en) * | 1993-11-16 | 1996-04-09 | Hybridon, Inc. | Method for detecting charged oligonucleotides in biological fluids |
CA2180723A1 (en) * | 1994-01-26 | 1995-08-03 | Aharon S. Cohen | Method of detecting sub-ppb levels of oligonucleotides in biological fluids |
US5766550A (en) * | 1995-03-15 | 1998-06-16 | City Of Hope | Disposable reagent storage and delivery cartridge |
US6613508B1 (en) | 1996-01-23 | 2003-09-02 | Qiagen Genomics, Inc. | Methods and compositions for analyzing nucleic acid molecules utilizing sizing techniques |
US6312893B1 (en) | 1996-01-23 | 2001-11-06 | Qiagen Genomics, Inc. | Methods and compositions for determining the sequence of nucleic acid molecules |
US6027890A (en) * | 1996-01-23 | 2000-02-22 | Rapigene, Inc. | Methods and compositions for enhancing sensitivity in the analysis of biological-based assays |
US5736626A (en) * | 1996-01-29 | 1998-04-07 | The Perkin-Elmer Corporation | Solid support reagents for the direct synthesis of 3'-labeled polynucleotides |
US6020481A (en) | 1996-04-01 | 2000-02-01 | The Perkin-Elmer Corporation | Asymmetric benzoxanthene dyes |
US6080852A (en) | 1996-06-27 | 2000-06-27 | The Perkin-Elmer Corporation | 4,7-dichlororhodamine dyes |
US7550570B2 (en) * | 2000-05-25 | 2009-06-23 | Applied Biosystems, Llc. | 4,7-dichlororhodamine dyes labeled polynucleotides |
US5874554A (en) * | 1996-12-13 | 1999-02-23 | Incyte Pharmaceuticals, Inc. | Methods and solvent vehicles for reagent delivery in oligonucleotide synthesis using automated pulse jetting devices |
US5902881A (en) * | 1997-03-03 | 1999-05-11 | Isis Pharmaceuticals, Inc. | Reagent useful for synthesizing sulfurized oligonucleotide analogs |
US5760209A (en) * | 1997-03-03 | 1998-06-02 | Isis Pharmaceuticals, Inc. | Protecting group for synthesizing oligonucleotide analogs |
US5756705A (en) * | 1997-03-05 | 1998-05-26 | Wang; Edge Renfeng | Method for labeling oligonucleotide with ammonia-sensitive ligands |
US6054576A (en) * | 1997-10-02 | 2000-04-25 | Ribozyme Pharmaceuticals, Inc. | Deprotection of RNA |
US6255476B1 (en) | 1999-02-22 | 2001-07-03 | Pe Corporation (Ny) | Methods and compositions for synthesis of labelled oligonucleotides and analogs on solid-supports |
AU2998800A (en) | 1999-03-24 | 2000-10-09 | Government Of The United States Of America, As Represented By The Secretary Of The Department Of Health And Human Services, The | N-acylphosphoramidites and their use in oligonucleotide synthesis |
CA2375060A1 (en) * | 1999-05-24 | 2000-11-30 | Invitrogen Corporation | Method for deblocking of labeled oligonucleotides |
US6750357B1 (en) | 1999-06-25 | 2004-06-15 | Syngen, Inc. | Rhodamine-based fluorophores useful as labeling reagents |
US7019129B1 (en) * | 2000-05-09 | 2006-03-28 | Biosearch Technologies, Inc. | Dark quenchers for donor-acceptor energy transfer |
AU2001295005A1 (en) * | 2000-08-11 | 2002-02-25 | Nanogen, Inc. | Macromolecules having hydrazide attachment moieties and reagents for their production |
US7101999B2 (en) | 2000-09-06 | 2006-09-05 | Evotec Ag | Oxazine derivatives |
KR20040016826A (ko) * | 2000-12-05 | 2004-02-25 | 아베시아 리미티드 | 포스포로티오에이트 올리고뉴클레오티드의 제조방법 |
US6768005B2 (en) | 2000-12-20 | 2004-07-27 | Avecia Limited | Process |
WO2002048385A2 (en) | 2000-12-12 | 2002-06-20 | Invitrogen Corporation | Compositions and methods for the release of nucleic acid molecules from solid matrices |
DE60207995T2 (de) * | 2001-03-08 | 2006-08-03 | Applera Corp., Foster City | Verfahren zum entschützen von oligonukleotiden |
CA2440322C (en) * | 2001-03-23 | 2014-09-09 | Geron Corporation | Oligonucleotide conjugates |
US7563618B2 (en) * | 2001-03-23 | 2009-07-21 | Geron Corporation | Oligonucleotide conjugates |
US20030082547A1 (en) | 2001-08-27 | 2003-05-01 | Ewing Gregory J. | Non-fluorescent quencher compounds and biomolecular assays |
AU2002353001A1 (en) | 2001-12-03 | 2003-06-17 | The Government Of The United States Of America, Represented By The Secretary Of The Department Of He | Thermolabile hydroxyl protecting groups and methods of use |
US20050165558A1 (en) * | 2002-02-26 | 2005-07-28 | Becker Robert G. | Sequence detection system calculator |
US9809824B2 (en) | 2004-12-13 | 2017-11-07 | The United States Of America, Represented By The Secretary, Department Of Health And Human Services | CpG oligonucleotide prodrugs, compositions thereof and associated therapeutic methods |
CA2647827A1 (en) * | 2006-03-31 | 2007-10-11 | Applied Biosystems Inc. | Reagents useful for synthesizing rhodamine-labeled oligonucleotides |
EP2036897B1 (de) * | 2007-09-04 | 2017-10-18 | Roche Diagnostics GmbH | Stabiles Reagens zur Rhodaminmarkierung |
EP2268608A4 (de) * | 2008-04-01 | 2012-01-11 | Biosearch Technologies Inc | Sonden für stabilisierte nukleinsäure-dark-quencher-fluorophore |
WO2013078244A1 (en) * | 2011-11-21 | 2013-05-30 | Promega Corporation | Carboxy x rhodamine analogs |
CN111704644B (zh) * | 2020-08-18 | 2020-12-04 | 苏州金唯智生物科技有限公司 | 一种氨解液及氨解方法 |
CN114671921A (zh) * | 2022-05-06 | 2022-06-28 | 南京吉盛澳玛生物医药有限公司 | 一种大规模寡聚核苷酸氨解方法 |
CN115850350A (zh) * | 2022-12-22 | 2023-03-28 | 苏州泓迅生物科技股份有限公司 | 一种氨解液及氨解方法 |
CN116102599A (zh) * | 2022-12-29 | 2023-05-12 | 苏州梓熙生物科技有限公司 | 寡核苷酸氨解方法 |
Family Cites Families (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4415732A (en) * | 1981-03-27 | 1983-11-15 | University Patents, Inc. | Phosphoramidite compounds and processes |
US4507433A (en) * | 1983-10-07 | 1985-03-26 | The Johns Hopkins University | Preparation of oligodeoxyribonucleoside alkyl or arylphosphonates |
FR2556726B1 (fr) * | 1983-12-20 | 1987-02-20 | California Inst Of Techn | Compositions a base d'oligonucleotides monocatenaires et procede pour leur preparation |
US4849513A (en) * | 1983-12-20 | 1989-07-18 | California Institute Of Technology | Deoxyribonucleoside phosphoramidites in which an aliphatic amino group is attached to the sugar ring and their use for the preparation of oligonucleotides containing aliphatic amino groups |
JPS622164A (ja) * | 1985-06-25 | 1987-01-08 | シスカ・ダイアグノステイツクス・インコ−ポレ−テツド | N4−(置換アミノ)シチジンを含む核酸プロ−ブ |
US4659774A (en) * | 1985-11-01 | 1987-04-21 | American Hoechst Corporation | Support for solid-phase oligonucleotide synthesis |
US4855225A (en) * | 1986-02-07 | 1989-08-08 | Applied Biosystems, Inc. | Method of detecting electrophoretically separated oligonucleotides |
DE3642939A1 (de) * | 1986-06-03 | 1987-12-10 | Europ Lab Molekularbiolog | Verfahren zur dna-markierung |
EP0972779A3 (de) * | 1987-10-28 | 2004-10-20 | Howard Florey Institute Of Experimental Physiology And Medicine | Oligonucleotid-Polyamid Konjugate |
-
1987
- 1987-12-24 US US07/138,287 patent/US4965349A/en not_active Expired - Lifetime
-
1988
- 1988-12-21 JP JP63320745A patent/JP2787775B2/ja not_active Expired - Fee Related
- 1988-12-22 EP EP94107016A patent/EP0617047B1/de not_active Revoked
- 1988-12-22 EP EP88312206A patent/EP0323152B1/de not_active Expired - Lifetime
- 1988-12-22 DE DE3853060T patent/DE3853060T2/de not_active Expired - Fee Related
- 1988-12-22 DE DE3856481T patent/DE3856481T2/de not_active Expired - Fee Related
Also Published As
Publication number | Publication date |
---|---|
EP0323152A2 (de) | 1989-07-05 |
DE3856481T2 (de) | 2002-04-18 |
DE3856481D1 (de) | 2001-08-23 |
EP0617047B1 (de) | 2001-07-18 |
EP0323152B1 (de) | 1995-02-15 |
US4965349A (en) | 1990-10-23 |
EP0323152A3 (de) | 1991-04-10 |
DE3853060T2 (de) | 1995-10-19 |
JPH02796A (ja) | 1990-01-05 |
EP0617047A1 (de) | 1994-09-28 |
JP2787775B2 (ja) | 1998-08-20 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
8364 | No opposition during term of opposition | ||
8327 | Change in the person/name/address of the patent owner |
Owner name: THE PERKIN-ELMER CORP. (N.D.GES.D.STAATES NEW YORK |
|
8327 | Change in the person/name/address of the patent owner |
Owner name: APPLERA CORP., FOSTER CITY, CALIF., US |
|
8339 | Ceased/non-payment of the annual fee |