DE3650783D1 - Verwendung des Glucoamylasepromotors aus Apergillus - Google Patents

Verwendung des Glucoamylasepromotors aus Apergillus

Info

Publication number
DE3650783D1
DE3650783D1 DE3650783T DE3650783T DE3650783D1 DE 3650783 D1 DE3650783 D1 DE 3650783D1 DE 3650783 T DE3650783 T DE 3650783T DE 3650783 T DE3650783 T DE 3650783T DE 3650783 D1 DE3650783 D1 DE 3650783D1
Authority
DE
Germany
Prior art keywords
coding regions
expression
foreign
aspergillus
secretion
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE3650783T
Other languages
English (en)
Other versions
DE3650783T2 (de
Inventor
David Ivor Gwynne
Francis Paul Buxton
Mark Hudson Pickett
Roger Wayne Davies
Claudio Scazzocchio
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
DSM IP Assets BV
Original Assignee
DSM IP Assets BV
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US06/811,404 external-priority patent/US5198345A/en
Application filed by DSM IP Assets BV filed Critical DSM IP Assets BV
Publication of DE3650783D1 publication Critical patent/DE3650783D1/de
Application granted granted Critical
Publication of DE3650783T2 publication Critical patent/DE3650783T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C12N9/2422—Alpha-amylase (3.2.1.1.) from plant source
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/555—Interferons [IFN]
    • C07K14/56—IFN-alpha
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2428—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01003—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/036—Fusion polypeptide containing a localisation/targetting motif targeting to the medium outside of the cell, e.g. type III secretion
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
    • C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Toxicology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Mycology (AREA)
  • Botany (AREA)
  • Plant Pathology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Yarns And Mechanical Finishing Of Yarns Or Ropes (AREA)
  • Mushroom Cultivation (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Mechanical Treatment Of Semiconductor (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Treatment Of Sludge (AREA)
  • Looms (AREA)
DE3650783T 1985-04-15 1986-04-14 Verwendung des Glucoamylasepromotors aus Apergillus Expired - Lifetime DE3650783T2 (de)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
CA479135 1985-04-15
CA479135 1985-04-15
US06/811,404 US5198345A (en) 1985-04-15 1985-12-20 Vectors in use in filamentous fungi
US811404 1997-03-04

Publications (2)

Publication Number Publication Date
DE3650783D1 true DE3650783D1 (de) 2003-12-04
DE3650783T2 DE3650783T2 (de) 2004-07-15

Family

ID=25670651

Family Applications (2)

Application Number Title Priority Date Filing Date
DE3650664T Expired - Fee Related DE3650664T2 (de) 1985-04-15 1986-04-14 Verwendung von promotoren von filamentösen pilzen
DE3650783T Expired - Lifetime DE3650783T2 (de) 1985-04-15 1986-04-14 Verwendung des Glucoamylasepromotors aus Apergillus

Family Applications Before (1)

Application Number Title Priority Date Filing Date
DE3650664T Expired - Fee Related DE3650664T2 (de) 1985-04-15 1986-04-14 Verwendung von promotoren von filamentösen pilzen

Country Status (6)

Country Link
EP (1) EP0284603B1 (de)
JP (1) JP2868179B2 (de)
AT (2) ATE253119T1 (de)
AU (1) AU606033B2 (de)
DE (2) DE3650664T2 (de)
WO (1) WO1986006097A1 (de)

Families Citing this family (45)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8508800D0 (en) * 1985-04-04 1985-05-09 Glaxo Group Ltd Microbiological process
US5364770A (en) * 1985-08-29 1994-11-15 Genencor International Inc. Heterologous polypeptides expressed in aspergillus
ATE117020T1 (de) * 1985-08-29 1995-01-15 Genencor Int Expression von heterologen polypeptiden in filamentösen pilzen, verfahren zu deren herstellung und vektoren zu deren herstellung.
US6004785A (en) * 1985-08-29 1999-12-21 Genencor International Inc. Heterologous polypeptides expressed in filamentous fungi, processes for making same, and vectors for making same
US4935349A (en) * 1986-01-17 1990-06-19 Zymogenetics, Inc. Expression of higher eucaryotic genes in aspergillus
US5536661A (en) * 1987-03-10 1996-07-16 Novo Nordisk A/S Process for the production of protein products in aspergillus
US5766912A (en) * 1986-03-17 1998-06-16 Novo Nordisk A/S Humicola lipase produced in aspergillus
FI872102A (fi) * 1986-06-06 1987-12-07 Panlabs Inc Expressionssystem hos filamentala fungi.
US5447862A (en) * 1987-02-04 1995-09-05 Ciba-Geigy Corporation Pectin lyase genes of aspergillus niger
GB8702475D0 (en) 1987-02-04 1987-03-11 Ciba Geigy Ag Expression system
US5252726A (en) * 1987-09-04 1993-10-12 Novo Nordisk A/S Promoters for use in aspergillus
WO1989001969A1 (en) * 1987-09-04 1989-03-09 Novo-Nordisk A/S Process for the production of protein products in aspergillus and promoters for use in aspergillus
ES2063162T5 (es) * 1988-07-28 2002-11-16 Novartis Ag Nuevo sistema de expresion.
US5162228A (en) * 1988-12-28 1992-11-10 Takeda Chemical Industries, Ltd. Gylceraldehyde-3-phosphate dehydrogenase gene and promoter
AU627334B2 (en) * 1989-06-16 1992-08-20 Genencor Inc. Dna sequences, vectors, and fusion polypeptides to increase secretion of desired polypeptides from filamentous fungi
JP2995770B2 (ja) * 1989-12-15 1999-12-27 吉富製薬株式会社 異種蛋白質の製造方法
WO1991013971A1 (en) * 1990-03-13 1991-09-19 Hawaii Biotechnology Group, Inc. Neurospora expression system
JP3478396B2 (ja) * 1992-03-04 2003-12-15 ジェネンコア インターナショナル インコーポレーテッド 黒色アスペルギルスカタラーゼ−ｒの産生
US5382658A (en) * 1992-04-03 1995-01-17 Allelix Biopharmaceuticals Inc. Stability-enhanced variants of parathyroid hormone
CA2098639A1 (en) * 1992-06-19 1993-12-20 K. Anne Kronis Bone stimulating, non-vasoactive parathyroid hormone variants
DK0673429T3 (da) * 1992-12-10 2002-10-07 Dsm Nv Fremstilling af heterologe proteiner i filamentøse svampe
GB9316883D0 (en) * 1993-08-13 1993-09-29 Univ Leeds Production of heterologous peptices
ES2080689B1 (es) * 1994-04-21 1996-09-01 Urquima Sa Procedimiento de obtencion de un edulcorante natural proteico.
GB9515941D0 (en) 1995-08-03 1995-10-04 Zeneca Ltd DNA constructs
DK0826054T3 (da) * 1995-05-18 2004-04-19 Genencor Int Ekspression af glycosyltransferase i Aspergillus
GB9907461D0 (en) 1999-03-31 1999-05-26 King S College London Neurite regeneration
US8372620B2 (en) 1998-12-23 2013-02-12 Dupont Nutrition Biosciences Aps Bacterial xylanases
GB0020498D0 (en) 2000-08-18 2000-10-11 Sterix Ltd Compound
AU7779800A (en) 2000-09-21 2002-04-02 Dsm Nv Talaromyces xylanases
CN100506981C (zh) 2000-12-07 2009-07-01 Dsmip资产有限公司 富含具有羧基末端脯氨酸残基的肽的蛋白质水解产物
ATE266087T1 (de) 2000-12-07 2004-05-15 Dsm Ip Assets Bv Verfahren zur verhinderung oder verminderung der trübung in getränken
GB0116453D0 (en) 2001-07-05 2001-08-29 Imp College Innovations Ltd Method
ES2525873T3 (es) 2002-06-07 2014-12-30 Dsm Ip Assets B.V. Método mejorado para la prevención o reducción del enturbiamiento en bebidas
TWI328612B (en) 2002-07-25 2010-08-11 Dsm Ip Assets Bv Genes and their encoded polypeptides involved in the biosynthetic pathway of vitamin b12, vectors and host cells comprising the genes, and process for producing vitamin b12
GB0403864D0 (en) 2004-02-20 2004-03-24 Ucl Ventures Modulator
BRPI0619381A2 (pt) 2005-11-28 2011-09-27 Dsm Ip Assets Bv preparações de enzimas que não alteram o paladar
GB0600406D0 (en) 2006-01-10 2006-02-15 Univ Bath Crystal
JP5436225B2 (ja) 2007-02-20 2014-03-05 ディーエスエム アイピー アセッツ ビー．ブイ． 新規シアリダーゼ
ES2608033T3 (es) 2007-03-22 2017-04-05 Dsm Ip Assets B.V. Nuevas lisil oxidasas
EP2604623A3 (de) 2007-08-08 2013-10-02 Novozymes Biopharma DK A/S Transferrinvarianten und Konjugate
WO2010089385A1 (en) 2009-02-06 2010-08-12 Novozymes Biopharma Dk A/S Purification process
EP3243835B1 (de) 2009-02-11 2024-04-10 Albumedix Ltd Varianten und konjugate von albumin
WO2011101339A2 (en) 2010-02-18 2011-08-25 Dsm Ip Assets B.V. Fermentation broth and filtration filter cake and uses thereof
WO2012007445A1 (en) 2010-07-13 2012-01-19 Dsm Ip Assets B.V. Mutarotase in crystallization
WO2012007446A1 (en) 2010-07-13 2012-01-19 Dsm Ip Assets B.V. Use of a mutarotase in the production of dried powders

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0099226A1 (de) * 1982-07-07 1984-01-25 St. Louis University Zur Replikation eines extrachromosomalen Elements befähigte fadenartige Pilze
NO840200L (no) * 1983-01-28 1984-07-30 Cefus Corp Glukoamylase cdna.
NZ207925A (en) * 1983-04-25 1988-05-30 Genentech Inc Yeast expression vehicle consisting of a yeast promoter and signal peptide encoding region linked to a heterologus peptide coding region; expression and culture
EP0136907A3 (de) * 1983-10-03 1986-12-30 Genentech, Inc. Xenogeneisches Expressionskontrollsystem, Verfahren zu dessen Verwendung, dieses enthaltende Expressionsvektoren, mit denselben transformierte Zellen und daraus hergestellte heterolose Proteine
EP0173378B1 (de) * 1984-07-27 1991-06-12 Unilever N.V. Verfahren zur Herstellung von einem Polypeptid mittels eines transformierten Mikroorganismus, ein dazu geeigneter transformierter Mikroorganismus, und zur Herstellung dieser Mikroorganismen geeignete DNS Sequenzen
EP0191221A1 (de) * 1984-10-24 1986-08-20 The Regents Of The University Of California Vektoren zur Transformation von Ascomyceten
US4753877A (en) * 1984-11-07 1988-06-28 Penalva Miguel A Cephalosporium acremonium
ATE117020T1 (de) * 1985-08-29 1995-01-15 Genencor Int Expression von heterologen polypeptiden in filamentösen pilzen, verfahren zu deren herstellung und vektoren zu deren herstellung.

Also Published As

Publication number Publication date
DE3650664D1 (de) 1998-02-12
EP0284603A1 (de) 1988-10-05
DE3650664T2 (de) 1998-04-16
JPH07163389A (ja) 1995-06-27
AU5698686A (en) 1986-11-05
WO1986006097A1 (en) 1986-10-23
ATE253119T1 (de) 2003-11-15
DE3650783T2 (de) 2004-07-15
AU606033B2 (en) 1991-01-31
EP0284603B1 (de) 1998-01-07
ATE161881T1 (de) 1998-01-15
JP2868179B2 (ja) 1999-03-10

Similar Documents

Publication Publication Date Title
DE3650783D1 (de) Verwendung des Glucoamylasepromotors aus Apergillus
EP0768383A3 (de) Promotoren von filamentösen Pilzen und deren Verwendung
ATE332378T1 (de) Polypeptide mit phytase wirkung
ATE196164T1 (de) Gereinigte scytalidium lacassen und nukleinsäuren dafür kodierend
ES2073571T3 (es) Aplicacion de nuevos fragmentos de adn como secuencia que codifica un peptido señal para la secrecion de proteinas maduras, mediante levaduras recombinantes, cassettes de expresion, levaduras transformadas y procedimiento correspondiente de preparacion de proteinas.
ES2054878T3 (es) Expresion de la proapolipoproteina a-i humana.
DE69633825D1 (de) Modifiziertes enzym mit lipolytischer aktivität
NO932423D0 (no) Ekspresjon av rekombinante proteiner i attenuerte bakterier
NO902082D0 (no) Fibronektinbindende protein samt fremstilling derav.
NZ219998A (en) Lysostaphin and its production by genetic engineering methods
IL69469A (en) Polypeptide having human h1-relaxin activity,double-stranded dna fragments coding therefor,vectors containing the dna fragments and methods for preparing the polypeptide,dna fragments and vectors
ES552234A0 (es) Un metodo para producir bacterianamente un polipeptido heteralogo que tiene actividad somatotropinica.
ATE364703T1 (de) Herstellung von proteinen aus pro-proteinen durch fusionsproteine abgeleitet von furin oder furinanalogen
GR852940B (de)
KR970700769A (ko) Aspergillus niger의 카르복시펩티다제, 재조합 단백질 및 그것의 제조방법(CARBOXYPEPTIDASE OF ASPERGILLUS NIGER, RECOMBINANT PROTEIN AND ITS METHOD OF PRODUCTION)
NO882380D0 (no) Fibfonektin-bindende protein, samt fremstilling derav.
WO1998013478A3 (en) Antifungal proteins, dna coding therefore, and hosts incorporating same
ATE170556T1 (de) Dna-sequenzen, vektoren und fusionspolypeptide zur erhöhung der sekretion gewünschter polypeptide von filamentösen fungi
DE69120390T2 (de) Kollagen-bindendes protein sowie dessen herstellung
ES2111537T3 (es) Inhibidor de la ribonucleasa de placenta humana recombinante y metodo de produccion.
NZ241280A (en) Recombinant iga protease with a modified non-functional c-terminal protein sequence
ES2086374T3 (es) Vectores de expresion de adn recombinante y compuestos de adn que codifican la actividad aciltransferasa de aspergillus.
DK580188D0 (da) Ekskpressionsplasmid, e.coli-vaertscelle og okse-somatotropin-lignende polypeptid
EP0183272A3 (en) Vector capable of efficient gene expression and utilization of such vector
PT100494A (pt) Vector de expressao dictiostelido e processo de expressao de uma proteina desejada

Legal Events

Date Code Title Description
8364 No opposition during term of opposition