DE3632343A1 - Verfahren zum nachweis von 1,2,2-trimethylpropylmethylphosphofluoridad - Google Patents
Verfahren zum nachweis von 1,2,2-trimethylpropylmethylphosphofluoridadInfo
- Publication number
- DE3632343A1 DE3632343A1 DE19863632343 DE3632343A DE3632343A1 DE 3632343 A1 DE3632343 A1 DE 3632343A1 DE 19863632343 DE19863632343 DE 19863632343 DE 3632343 A DE3632343 A DE 3632343A DE 3632343 A1 DE3632343 A1 DE 3632343A1
- Authority
- DE
- Germany
- Prior art keywords
- compound
- detected
- derivative
- detection
- protein
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000034 method Methods 0.000 title claims description 16
- 102000004169 proteins and genes Human genes 0.000 claims description 13
- 108090000623 proteins and genes Proteins 0.000 claims description 13
- 102000004190 Enzymes Human genes 0.000 claims description 9
- 108090000790 Enzymes Proteins 0.000 claims description 9
- 150000001875 compounds Chemical class 0.000 claims description 8
- 238000001514 detection method Methods 0.000 claims description 8
- 238000003018 immunoassay Methods 0.000 claims description 8
- YCIMNLLNPGFGHC-UHFFFAOYSA-N catechol Chemical compound OC1=CC=CC=C1O YCIMNLLNPGFGHC-UHFFFAOYSA-N 0.000 claims description 6
- 102000003992 Peroxidases Human genes 0.000 claims description 3
- UKJLNMAFNRKWGR-UHFFFAOYSA-N cyclohexatrienamine Chemical class NC1=CC=C=C[CH]1 UKJLNMAFNRKWGR-UHFFFAOYSA-N 0.000 claims description 3
- 108040007629 peroxidase activity proteins Proteins 0.000 claims description 3
- 229960000814 tetanus toxoid Drugs 0.000 claims description 3
- 241000700198 Cavia Species 0.000 claims description 2
- AVXURJPOCDRRFD-UHFFFAOYSA-N Hydroxylamine Chemical compound ON AVXURJPOCDRRFD-UHFFFAOYSA-N 0.000 claims description 2
- 241000283973 Oryctolagus cuniculus Species 0.000 claims description 2
- 230000003647 oxidation Effects 0.000 claims description 2
- 238000007254 oxidation reaction Methods 0.000 claims description 2
- KHIWWQKSHDUIBK-UHFFFAOYSA-N periodic acid Chemical compound OI(=O)(=O)=O KHIWWQKSHDUIBK-UHFFFAOYSA-N 0.000 claims description 2
- 239000004593 Epoxy Chemical class 0.000 claims 1
- 125000004103 aminoalkyl group Chemical class 0.000 claims 1
- -1 aminophenyl Chemical class 0.000 claims 1
- 125000004181 carboxyalkyl group Chemical class 0.000 claims 1
- 108060003552 hemocyanin Proteins 0.000 claims 1
- 229940088598 enzyme Drugs 0.000 description 6
- 238000006243 chemical reaction Methods 0.000 description 4
- 238000011161 development Methods 0.000 description 3
- 230000018109 developmental process Effects 0.000 description 3
- 150000003384 small molecules Chemical class 0.000 description 3
- 125000005919 1,2,2-trimethylpropyl group Chemical group 0.000 description 2
- 239000000427 antigen Substances 0.000 description 2
- 230000000890 antigenic effect Effects 0.000 description 2
- 102000036639 antigens Human genes 0.000 description 2
- 108091007433 antigens Proteins 0.000 description 2
- 238000003556 assay Methods 0.000 description 2
- 150000002148 esters Chemical class 0.000 description 2
- 238000011896 sensitive detection Methods 0.000 description 2
- QYKVBHFKNHUXPY-UHFFFAOYSA-N 4-[3,3-dimethylbutan-2-yloxy(methyl)phosphoryl]oxyaniline Chemical compound CC(C)(C)C(C)OP(C)(=O)OC1=CC=C(N)C=C1 QYKVBHFKNHUXPY-UHFFFAOYSA-N 0.000 description 1
- 102000012440 Acetylcholinesterase Human genes 0.000 description 1
- 108010022752 Acetylcholinesterase Proteins 0.000 description 1
- 238000002965 ELISA Methods 0.000 description 1
- SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical compound O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 description 1
- WTKZEGDFNFYCGP-UHFFFAOYSA-N Pyrazole Chemical class C=1C=NNC=1 WTKZEGDFNFYCGP-UHFFFAOYSA-N 0.000 description 1
- 229940022698 acetylcholinesterase Drugs 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 150000008064 anhydrides Chemical class 0.000 description 1
- 150000001718 carbodiimides Chemical class 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- AOGYCOYQMAVAFD-UHFFFAOYSA-N chlorocarbonic acid Chemical class OC(Cl)=O AOGYCOYQMAVAFD-UHFFFAOYSA-N 0.000 description 1
- 230000002860 competitive effect Effects 0.000 description 1
- 238000001212 derivatisation Methods 0.000 description 1
- 125000005442 diisocyanate group Chemical group 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 229910052731 fluorine Inorganic materials 0.000 description 1
- 125000001153 fluoro group Chemical group F* 0.000 description 1
- 125000000524 functional group Chemical group 0.000 description 1
- 238000004817 gas chromatography Methods 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 230000001900 immune effect Effects 0.000 description 1
- 230000028993 immune response Effects 0.000 description 1
- 230000002163 immunogen Effects 0.000 description 1
- 230000016784 immunoglobulin production Effects 0.000 description 1
- 239000003112 inhibitor Substances 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 239000002917 insecticide Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000004949 mass spectrometry Methods 0.000 description 1
- 150000003014 phosphoric acid esters Chemical class 0.000 description 1
- 238000011197 physicochemical method Methods 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 239000007790 solid phase Substances 0.000 description 1
- 125000006850 spacer group Chemical group 0.000 description 1
- 239000007858 starting material Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/531—Production of immunochemical test materials
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/5308—Immunoassay; Biospecific binding assay; Materials therefor for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Food Science & Technology (AREA)
- Biochemistry (AREA)
- Cell Biology (AREA)
- Biotechnology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
Priority Applications (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE19863632343 DE3632343A1 (de) | 1986-09-24 | 1986-09-24 | Verfahren zum nachweis von 1,2,2-trimethylpropylmethylphosphofluoridad |
| IL83624A IL83624A0 (en) | 1986-09-24 | 1987-08-23 | Method of detecting pinacolyl methylphosphonofluoridate(soman) |
| EP87112233A EP0261416A3 (de) | 1986-09-24 | 1987-08-24 | Verfahren zum Nachweis von 1,2,2-Trimethylpropylmethylphosphofluoridad |
| JP62239919A JPS6396556A (ja) | 1986-09-24 | 1987-09-24 | ピナコリルメチルホスホノフルオリデート（ソマン）の検出法 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE19863632343 DE3632343A1 (de) | 1986-09-24 | 1986-09-24 | Verfahren zum nachweis von 1,2,2-trimethylpropylmethylphosphofluoridad |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DE3632343A1 true DE3632343A1 (de) | 1988-04-07 |
| DE3632343C2 DE3632343C2 (enExample) | 1989-01-05 |
Family
ID=6310181
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE19863632343 Granted DE3632343A1 (de) | 1986-09-24 | 1986-09-24 | Verfahren zum nachweis von 1,2,2-trimethylpropylmethylphosphofluoridad |
Country Status (4)
| Country | Link |
|---|---|
| EP (1) | EP0261416A3 (enExample) |
| JP (1) | JPS6396556A (enExample) |
| DE (1) | DE3632343A1 (enExample) |
| IL (1) | IL83624A0 (enExample) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1990010709A2 (en) * | 1989-03-14 | 1990-09-20 | Board Of Regents Of The University Of Nebraska | Monoclonal antibodies for metallic cations on small molecules |
| US5639624A (en) * | 1989-03-14 | 1997-06-17 | Board Of Regents Of The University Of Nebraska | Monoclonal antibodies specific for metallic cations and method therefor |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA1256795A (en) * | 1983-12-28 | 1989-07-04 | Dennis A. Carson | Anti-idiotype antibodies induced by synthetic polypeptides |
| DE3400434A1 (de) * | 1984-01-09 | 1985-09-26 | Behringwerke Ag, 3550 Marburg | Bestimmung von fibrin mit fibrin-spezifischen antikoerpern |
| DE3515901A1 (de) * | 1985-05-03 | 1986-11-06 | Battelle-Institut E.V., 6000 Frankfurt | Verfahren zum nachweis von 1, 2, 2-trimethylpropylmethylphosphofluoridad |
-
1986
- 1986-09-24 DE DE19863632343 patent/DE3632343A1/de active Granted
-
1987
- 1987-08-23 IL IL83624A patent/IL83624A0/xx unknown
- 1987-08-24 EP EP87112233A patent/EP0261416A3/de not_active Withdrawn
- 1987-09-24 JP JP62239919A patent/JPS6396556A/ja active Pending
Non-Patent Citations (1)
| Title |
|---|
| FEBS Letters, Vol. 149, Nr. 1, Nov. 1982, S. 147-151 * |
Also Published As
| Publication number | Publication date |
|---|---|
| EP0261416A3 (de) | 1989-02-01 |
| EP0261416A2 (de) | 1988-03-30 |
| JPS6396556A (ja) | 1988-04-27 |
| DE3632343C2 (enExample) | 1989-01-05 |
| IL83624A0 (en) | 1988-01-31 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| OP8 | Request for examination as to paragraph 44 patent law | ||
| D2 | Grant after examination | ||
| 8364 | No opposition during term of opposition | ||
| 8327 | Change in the person/name/address of the patent owner |
Owner name: SAILER, JOHANN, DR., 53359 RHEINBACH, DE |
|
| 8339 | Ceased/non-payment of the annual fee |