DE3571646D1 - Process for the amplification of the expression of a determined gene in bacillus subtilis and strains obtained - Google Patents

Process for the amplification of the expression of a determined gene in bacillus subtilis and strains obtained

Info

Publication number
DE3571646D1
DE3571646D1 DE8585400830T DE3571646T DE3571646D1 DE 3571646 D1 DE3571646 D1 DE 3571646D1 DE 8585400830 T DE8585400830 T DE 8585400830T DE 3571646 T DE3571646 T DE 3571646T DE 3571646 D1 DE3571646 D1 DE 3571646D1
Authority
DE
Germany
Prior art keywords
gene
integration
bacillus
strains obtained
amplification
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
DE8585400830T
Other languages
English (en)
Inventor
Stanilas Ehrlich
Laurent Janniere
Evelyne Pierre
Brigitte Niaudet
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Centre National de la Recherche Scientifique CNRS
Original Assignee
Centre National de la Recherche Scientifique CNRS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=9303577&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=DE3571646(D1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Centre National de la Recherche Scientifique CNRS filed Critical Centre National de la Recherche Scientifique CNRS
Application granted granted Critical
Publication of DE3571646D1 publication Critical patent/DE3571646D1/de
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/822—Microorganisms using bacteria or actinomycetales
    • Y10S435/832—Bacillus
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/822—Microorganisms using bacteria or actinomycetales
    • Y10S435/832—Bacillus
    • Y10S435/839—Bacillus subtilis
DE8585400830T 1984-04-27 1985-04-26 Process for the amplification of the expression of a determined gene in bacillus subtilis and strains obtained Expired DE3571646D1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR8406701A FR2563533B1 (fr) 1984-04-27 1984-04-27 Procede d'amplification de l'expression d'un gene determine chez bacillus subtilis et souches obtenues

Publications (1)

Publication Number Publication Date
DE3571646D1 true DE3571646D1 (en) 1989-08-24

Family

ID=9303577

Family Applications (1)

Application Number Title Priority Date Filing Date
DE8585400830T Expired DE3571646D1 (en) 1984-04-27 1985-04-26 Process for the amplification of the expression of a determined gene in bacillus subtilis and strains obtained

Country Status (6)

Country Link
US (1) US4959316A (de)
EP (1) EP0166628B1 (de)
JP (1) JPH0665306B2 (de)
AT (1) ATE44766T1 (de)
DE (1) DE3571646D1 (de)
FR (1) FR2563533B1 (de)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2637532B2 (ja) * 1987-02-27 1997-08-06 ギスト ブロカデス ナームローゼ フェンノートチャップ 原核微生物の染色体ｄｎａにおける安定した遺伝子増幅
FR2627508B1 (fr) * 1988-02-22 1990-10-05 Eurolysine Procede pour l'integration d'un gene choisi sur le chromosome d'une bacterie et bacterie obtenue par ledit procede
JPH02211862A (ja) * 1988-06-22 1990-08-23 Toagosei Chem Ind Co Ltd 抗昆虫蛋白産生枯草菌
JPH0669365B2 (ja) * 1989-05-22 1994-09-07 株式会社ミドリ十字 アルブミン遺伝子を含むプラスミド、形質転換体、形質転換体の製造方法、アルブミンの製造方法
CA2017176A1 (en) * 1989-05-22 1990-11-22 Yoshitomi Pharmaceutical Industries Ltd. Albumin gene-containing plasmid, transformant carrying same, production of such transformant and production of albumin
FR2650292B1 (fr) * 1989-07-27 1994-03-04 Institut Recherche Agronomique Procede de production de proteine a partir de souches de bacillus subtilis et procede de preparation desdites souches
US5695976A (en) * 1989-12-18 1997-12-09 Novo Nordisk A/S Stable integration of DNA in bacterial genomes
DE4216246A1 (de) * 1992-05-16 1993-11-18 Solvay Enzymes Gmbh & Co Kg Einfaches Verfahren zur stabilen chromosomalen Genamplifikation
DK153992D0 (da) * 1992-12-22 1992-12-22 Novo Nordisk As Metode
US5733753A (en) * 1992-12-22 1998-03-31 Novo Nordisk A/S Amplification of genomic DNA by site specific integration of a selectable marker construct
US5861273A (en) * 1993-12-21 1999-01-19 Celtrix Phamraceuticals, Inc. Chromosomal expression of heterologous genes in bacterial cells
CN1307642A (zh) * 1998-05-27 2001-08-08 诺沃诺尔迪斯克生物技术有限公司 通过改变基因拷贝数制备多肽的方法
KR20010111577A (ko) * 1999-03-23 2001-12-19 보드 오브 트러스티즈 오퍼래이팅 미시건 스태이트 유니버시티 미오-이노시톨-1-포스페이트 신타아제 및미오-이노시톨-2-디히드로게나아제를 사용한1,2,3-트리히드록시벤젠 및 1,2,3,4-테트라히드록시벤젠의합성
CN101061214B (zh) 2004-10-22 2012-12-05 诺维信公司 多个多核苷酸拷贝的稳定基因组整合

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4336336A (en) * 1979-01-12 1982-06-22 President And Fellows Of Harvard College Fused gene and method of making and using same
GB2068971B (en) * 1980-01-30 1983-06-08 Searle & Co Recombinant dna techniques
EP0074808A3 (de) * 1981-09-16 1984-07-04 University Patents, Inc. Rekombinantes Verfahren und Materialien
JPS59501195A (ja) * 1982-07-09 1984-07-12 ボイス　トンプソン　インスチチユ−ト　フオ−プラント　リサ−チ，インコ−ポレ−テツド 原核生物における遺伝子工学

Also Published As

Publication number Publication date
JPS60234579A (ja) 1985-11-21
EP0166628B1 (de) 1989-07-19
ATE44766T1 (de) 1989-08-15
FR2563533A1 (fr) 1985-10-31
JPH0665306B2 (ja) 1994-08-24
US4959316A (en) 1990-09-25
EP0166628A1 (de) 1986-01-02
FR2563533B1 (fr) 1986-08-22

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Legal Events

Date Code Title Description
8363 Opposition against the patent