ES2056410T3 - Procedimiento de produccion a partir de cepas de bacillus subtilis y procedimiento de preparacion de dichas cepas. - Google Patents

Procedimiento de produccion a partir de cepas de bacillus subtilis y procedimiento de preparacion de dichas cepas.

Info

Publication number
ES2056410T3
ES2056410T3 ES90402172T ES90402172T ES2056410T3 ES 2056410 T3 ES2056410 T3 ES 2056410T3 ES 90402172 T ES90402172 T ES 90402172T ES 90402172 T ES90402172 T ES 90402172T ES 2056410 T3 ES2056410 T3 ES 2056410T3
Authority
ES
Spain
Prior art keywords
gene
protein
induced
strain
strains
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES90402172T
Other languages
English (en)
Inventor
Gwennael Joliff
Marie-Agnes Petit
Juan Manuel Mesas
Andre Klier
Georges Rapoport
Dusko Stanislas Ehrlich
Phillipe Looten
Didier Vernade
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Institut Pasteur de Lille
Institut National de la Recherche Agronomique INRA
Original Assignee
Institut Pasteur de Lille
Institut National de la Recherche Agronomique INRA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Institut Pasteur de Lille, Institut National de la Recherche Agronomique INRA filed Critical Institut Pasteur de Lille
Application granted granted Critical
Publication of ES2056410T3 publication Critical patent/ES2056410T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01004—Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/07—Bacillus
    • C12R2001/125—Bacillus subtilis ; Hay bacillus; Grass bacillus

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

LA PRESENTE INVENCION TIENE POR OBJETO LA OBTENCION DE UNA PROTEINA, CARACTERIZADA PORQUE A PARTIR DE UNA CEPA DEL GENERO BACILLUS EN EL CROMOSOMA DEL CUAL HAN SIDO INTEGRADOS EN UN GEN NO ESENCIAL DE DICHA CEPA: A) UNA UNIDAD AMPLIABLE QUE TIENEN UNA SECUENCIA DE ADN DETERMINADA M ENCUADRADA POR DOS SECUENCIAS DE ADN IDENTICAS D ORIENTADAS EN SENTIDO DIRECTO, CONTENIENDO LA SECUENCIA M EL GEN DE ESTRUCTURA DE DICHA PROTEINA PUESTO BAJO EL CONTROL DE ELEMENTOS DE EXPRESION Y SECRECION INDUCTIBLES EN BACILLUS DE DICHA PROTEINA Y EVENTUALMENTE UN GEN SELECIONABLE, Y B) UN SISTEMA DE REPLICACION O REPLICON INDUCTIBLE O CONDICIONAL IGUALMENTE INTEGRADO EN DICHO GEN NO ESENCIAL DEL CROMOSOMA DE LA CEPA SUPERIOR DE LA UNIDAD AMPLIABLE; 1. SE SELECCIONAN EVENTUALMENTE LOS CLONES AMPLIADOS OBTENIDOS POR CULTIVO EN UN MEDIO DE SELECCCION QUE CORRESPONDE AL GEN SELECCIONABLE Y SE TOMAN LAS CEPAS CUYO GENOMA CORRESPONDE A LA PRESENCIA DE UN NUMERO DE COPIAS DE DICHO GEN AUMENTADO RESPECTO A LA POBLACION BACTERIANA ANTES DE SELECCION; Y 2. EN UN MEDIO DE CULTIVO ADECUADO, SE INDUCE O CONDICIONA UNA AMPLIACION GENICA POR ACTIVACION DEL REPLICON, Y SE INDUCE EN LA EXPRESION Y LA SECRECION DE LA PROTEINA LA ACTIVACION DE DICHOS ELEMENTOS DE EXPRESION; 3. SE RECUPERA DICHA PROTEINA EXPRESADA. LA PRESENTE INVENCION TIENE IGUALMENTE POR OBJETO UN PROCEDIMIENTO PARA LA PREPARACION DE UNA CEPA DE BACILLUS AMPLIADA O NO, UTILIZADA ESPECIALMENTE EN EL PROCEDIMIENTO DE PRODUCCION SEGUN LA INVENCION.
ES90402172T 1989-07-27 1990-07-27 Procedimiento de produccion a partir de cepas de bacillus subtilis y procedimiento de preparacion de dichas cepas. Expired - Lifetime ES2056410T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR898910143A FR2650292B1 (fr) 1989-07-27 1989-07-27 Procede de production de proteine a partir de souches de bacillus subtilis et procede de preparation desdites souches

Publications (1)

Publication Number Publication Date
ES2056410T3 true ES2056410T3 (es) 1994-10-01

Family

ID=9384203

Family Applications (1)

Application Number Title Priority Date Filing Date
ES90402172T Expired - Lifetime ES2056410T3 (es) 1989-07-27 1990-07-27 Procedimiento de produccion a partir de cepas de bacillus subtilis y procedimiento de preparacion de dichas cepas.

Country Status (6)

Country Link
EP (1) EP0410895B1 (es)
AT (1) ATE105866T1 (es)
DE (1) DE69008959T2 (es)
DK (1) DK0410895T3 (es)
ES (1) ES2056410T3 (es)
FR (1) FR2650292B1 (es)

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2563533B1 (fr) * 1984-04-27 1986-08-22 Centre Nat Rech Scient Procede d'amplification de l'expression d'un gene determine chez bacillus subtilis et souches obtenues
FR2582316B1 (fr) * 1985-05-22 1988-12-02 Centre Nat Rech Scient Vecteurs d'expression de l'a-amylase dans bacillus subtilis, souches obtenues et procede de preparation d'a-amylase

Also Published As

Publication number Publication date
ATE105866T1 (de) 1994-06-15
DE69008959T2 (de) 1994-12-22
DK0410895T3 (da) 1994-10-03
EP0410895B1 (fr) 1994-05-18
FR2650292A1 (fr) 1991-02-01
EP0410895A1 (fr) 1991-01-30
DE69008959D1 (de) 1994-06-23
FR2650292B1 (fr) 1994-03-04

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