DE3440988A1 - Verfahren zur spaltung von peptiden und proteinen an der methionyl-bindung - Google Patents
Verfahren zur spaltung von peptiden und proteinen an der methionyl-bindungInfo
- Publication number
- DE3440988A1 DE3440988A1 DE19843440988 DE3440988A DE3440988A1 DE 3440988 A1 DE3440988 A1 DE 3440988A1 DE 19843440988 DE19843440988 DE 19843440988 DE 3440988 A DE3440988 A DE 3440988A DE 3440988 A1 DE3440988 A1 DE 3440988A1
- Authority
- DE
- Germany
- Prior art keywords
- reaction
- cleavage
- cyanogen chloride
- proteins
- methionyl
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
- 238000000034 method Methods 0.000 title claims abstract description 18
- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 16
- 102000004169 proteins and genes Human genes 0.000 title claims abstract description 15
- 108090000765 processed proteins & peptides Proteins 0.000 title claims abstract description 7
- 102000004196 processed proteins & peptides Human genes 0.000 title claims abstract description 6
- QPJDMGCKMHUXFD-UHFFFAOYSA-N cyanogen chloride Chemical compound ClC#N QPJDMGCKMHUXFD-UHFFFAOYSA-N 0.000 claims abstract description 28
- 238000003776 cleavage reaction Methods 0.000 claims abstract description 14
- 230000007017 scission Effects 0.000 claims abstract description 13
- BDAGIHXWWSANSR-UHFFFAOYSA-N methanoic acid Natural products OC=O BDAGIHXWWSANSR-UHFFFAOYSA-N 0.000 claims description 13
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 8
- 238000006243 chemical reaction Methods 0.000 claims description 8
- 235000019253 formic acid Nutrition 0.000 claims description 8
- 239000007789 gas Substances 0.000 claims description 8
- 239000011541 reaction mixture Substances 0.000 claims description 8
- OSWFIVFLDKOXQC-UHFFFAOYSA-N 4-(3-methoxyphenyl)aniline Chemical compound COC1=CC=CC(C=2C=CC(N)=CC=2)=C1 OSWFIVFLDKOXQC-UHFFFAOYSA-N 0.000 claims description 5
- 239000012429 reaction media Substances 0.000 claims description 5
- 239000000203 mixture Substances 0.000 claims description 4
- 229910052757 nitrogen Inorganic materials 0.000 claims description 4
- 239000002253 acid Substances 0.000 claims description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 3
- ATDGTVJJHBUTRL-UHFFFAOYSA-N cyanogen bromide Chemical compound BrC#N ATDGTVJJHBUTRL-UHFFFAOYSA-N 0.000 description 12
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 9
- 150000001413 amino acids Chemical group 0.000 description 6
- 102000005936 beta-Galactosidase Human genes 0.000 description 6
- 108010005774 beta-Galactosidase Proteins 0.000 description 6
- 238000004458 analytical method Methods 0.000 description 5
- 230000007026 protein scission Effects 0.000 description 4
- 108020004256 Beta-lactamase Proteins 0.000 description 3
- FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 description 3
- 229920004482 WACKER® Polymers 0.000 description 3
- 102000006635 beta-lactamase Human genes 0.000 description 3
- 238000007664 blowing Methods 0.000 description 3
- 239000012634 fragment Substances 0.000 description 3
- 102000037865 fusion proteins Human genes 0.000 description 3
- 108020001507 fusion proteins Proteins 0.000 description 3
- 229930182817 methionine Natural products 0.000 description 3
- VYHVQEYOFIYNJP-UHFFFAOYSA-N methyl thiocyanate Chemical compound CSC#N VYHVQEYOFIYNJP-UHFFFAOYSA-N 0.000 description 3
- 238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 description 3
- 239000007787 solid Substances 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 description 2
- 231100000636 lethal dose Toxicity 0.000 description 2
- 150000007524 organic acids Chemical class 0.000 description 2
- 230000035484 reaction time Effects 0.000 description 2
- 230000009257 reactivity Effects 0.000 description 2
- 231100000331 toxic Toxicity 0.000 description 2
- 230000002588 toxic effect Effects 0.000 description 2
- -1 Cyanogen Halides Chemical class 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 241000588724 Escherichia coli Species 0.000 description 1
- 102000018997 Growth Hormone Human genes 0.000 description 1
- 108010051696 Growth Hormone Proteins 0.000 description 1
- 108090001061 Insulin Proteins 0.000 description 1
- 102000004877 Insulin Human genes 0.000 description 1
- 102000014150 Interferons Human genes 0.000 description 1
- 108010050904 Interferons Proteins 0.000 description 1
- QIVBCDIJIAJPQS-VIFPVBQESA-N L-tryptophane Chemical compound C1=CC=C2C(C[C@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-VIFPVBQESA-N 0.000 description 1
- 229910019093 NaOCl Inorganic materials 0.000 description 1
- 108010076181 Proinsulin Proteins 0.000 description 1
- 238000012300 Sequence Analysis Methods 0.000 description 1
- QIVBCDIJIAJPQS-UHFFFAOYSA-N Tryptophan Natural products C1=CC=C2C(CC(N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-UHFFFAOYSA-N 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 238000005336 cracking Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 238000006911 enzymatic reaction Methods 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 230000008020 evaporation Effects 0.000 description 1
- 239000006260 foam Substances 0.000 description 1
- 238000004108 freeze drying Methods 0.000 description 1
- 230000004927 fusion Effects 0.000 description 1
- 238000001502 gel electrophoresis Methods 0.000 description 1
- 238000010353 genetic engineering Methods 0.000 description 1
- JMANVNJQNLATNU-UHFFFAOYSA-N glycolonitrile Natural products N#CC#N JMANVNJQNLATNU-UHFFFAOYSA-N 0.000 description 1
- 239000000122 growth hormone Substances 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 239000011261 inert gas Substances 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 229940125396 insulin Drugs 0.000 description 1
- 229940079322 interferon Drugs 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 235000005985 organic acids Nutrition 0.000 description 1
- 239000013612 plasmid Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 108010066381 preproinsulin Proteins 0.000 description 1
- 238000000164 protein isolation Methods 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- ZVCDLGYNFYZZOK-UHFFFAOYSA-M sodium cyanate Chemical compound [Na]OC#N ZVCDLGYNFYZZOK-UHFFFAOYSA-M 0.000 description 1
- SUKJFIGYRHOWBL-UHFFFAOYSA-N sodium hypochlorite Chemical compound [Na+].Cl[O-] SUKJFIGYRHOWBL-UHFFFAOYSA-N 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 230000002269 spontaneous effect Effects 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 231100000167 toxic agent Toxicity 0.000 description 1
- 239000003440 toxic substance Substances 0.000 description 1
- 231100000027 toxicology Toxicity 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/12—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by hydrolysis, i.e. solvolysis in general
- C07K1/126—Aminolysis
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/12—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by hydrolysis, i.e. solvolysis in general
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
- Y10S530/81—Carrier - bound or immobilized peptides or proteins and the preparation thereof, e.g. biological cell or cell fragment as carrier
- Y10S530/812—Peptides or proteins is immobilized on, or in, an organic carrier
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Medicinal Chemistry (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Life Sciences & Earth Sciences (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
- Saccharide Compounds (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Priority Applications (16)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE19843440988 DE3440988A1 (de) | 1984-11-09 | 1984-11-09 | Verfahren zur spaltung von peptiden und proteinen an der methionyl-bindung |
| AT85113857T ATE71109T1 (de) | 1984-11-09 | 1985-10-31 | Verfahren zur spaltung von peptiden und proteinen an der methionyl-bindung. |
| EP85113857A EP0180920B1 (de) | 1984-11-09 | 1985-10-31 | Verfahren zur Spaltung von Peptiden und Proteinen an der Methionyl-Bindung |
| DE8585113857T DE3585078D1 (de) | 1984-11-09 | 1985-10-31 | Verfahren zur spaltung von peptiden und proteinen an der methionyl-bindung. |
| GR852693A GR852693B (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) | 1984-11-09 | 1985-11-07 | |
| PT81445A PT81445B (pt) | 1984-11-09 | 1985-11-07 | Processo para a dissociacao de peptideos e proteinas na ligacao metionilo |
| FI854383A FI85494C (fi) | 1984-11-09 | 1985-11-07 | Foerfarande foer avspaltning av peptider och proteiner av metionyl-bindning. |
| US06/795,920 US4644057A (en) | 1984-11-09 | 1985-11-07 | Process for the cleavage of peptides and proteins at the methionyl bond using cyanogen chloride |
| ES548617A ES8701780A1 (es) | 1984-11-09 | 1985-11-07 | Procedimiento para disociar peptidos y proteinas en el enla-ce metionilo |
| JP60249148A JPH0714960B2 (ja) | 1984-11-09 | 1985-11-08 | ペプチドおよびタンパクのメチオニル結合での分裂方法 |
| DK516485A DK166546C (da) | 1984-11-09 | 1985-11-08 | Fremgangsmaade til spaltning af peptider og proteiner ved methionyl-bindingen |
| CA000494971A CA1247081A (en) | 1984-11-09 | 1985-11-08 | Process for the cleavage of peptides and proteins at the methionyl bond |
| IE279585A IE58683B1 (en) | 1984-11-09 | 1985-11-08 | A process for the cleavage of peptides and proteins at the methionyl bond |
| ZA858603A ZA858603B (en) | 1984-11-09 | 1985-11-08 | A process for the cleavage of peptides and proteins at the methionyl bond |
| AU49713/85A AU580348B2 (en) | 1984-11-09 | 1985-11-08 | A process for the cleavage of peptides and proteins at the methionyl bond |
| KR1019850008387A KR930007428B1 (ko) | 1984-11-09 | 1985-11-09 | 메티오닐 결합에서의 펩티드 및 단백질의 분해 방법 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE19843440988 DE3440988A1 (de) | 1984-11-09 | 1984-11-09 | Verfahren zur spaltung von peptiden und proteinen an der methionyl-bindung |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DE3440988A1 true DE3440988A1 (de) | 1986-07-10 |
Family
ID=6249917
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE19843440988 Withdrawn DE3440988A1 (de) | 1984-11-09 | 1984-11-09 | Verfahren zur spaltung von peptiden und proteinen an der methionyl-bindung |
| DE8585113857T Expired - Fee Related DE3585078D1 (de) | 1984-11-09 | 1985-10-31 | Verfahren zur spaltung von peptiden und proteinen an der methionyl-bindung. |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE8585113857T Expired - Fee Related DE3585078D1 (de) | 1984-11-09 | 1985-10-31 | Verfahren zur spaltung von peptiden und proteinen an der methionyl-bindung. |
Country Status (15)
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| LT3328B (en) | 1990-09-05 | 1995-07-25 | Hoechst Ag | Process for the preparation of insulin |
| WO2010016069A1 (en) | 2008-08-07 | 2010-02-11 | Biocon Limited | A process for preparation of insulin compounds |
Families Citing this family (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4745178A (en) * | 1987-04-22 | 1988-05-17 | Eli Lilly And Company | Process for selective peptide bond cleavage using sulfoxides and CF3 CO |
| DE3726655A1 (de) * | 1987-08-11 | 1989-02-23 | Hoechst Ag | Verfahren zur isolierung basischer proteine aus proteingemischen, welche solche basischen proteine enthalten |
| US4945157A (en) * | 1988-05-27 | 1990-07-31 | University Of Florida | Novel Extraction procedure for protein G |
| ATE264871T1 (de) | 1996-07-26 | 2004-05-15 | Aventis Pharma Gmbh | Insulinderivate mit erhöhter zinkbindung |
| DE19726167B4 (de) | 1997-06-20 | 2008-01-24 | Sanofi-Aventis Deutschland Gmbh | Insulin, Verfahren zu seiner Herstellung und es enthaltende pharmazeutische Zubereitung |
| DE19825447A1 (de) | 1998-06-06 | 1999-12-09 | Hoechst Marion Roussel De Gmbh | Neue Insulinanaloga mit erhöhter Zinkbildung |
| DE10114178A1 (de) | 2001-03-23 | 2002-10-10 | Aventis Pharma Gmbh | Zinkfreie und zinkarme Insulinzubereitungen mit verbesserter Stabilität |
| DE10227232A1 (de) | 2002-06-18 | 2004-01-15 | Aventis Pharma Deutschland Gmbh | Saure Insulinzubereitungen mit verbesserter Stabilität |
| US7193035B2 (en) * | 2002-10-29 | 2007-03-20 | Sanofi-Aventis Deutschland Gmbh | Crystals of insulin analogs and processes for their preparation |
| EP3677275B1 (de) | 2008-10-17 | 2024-06-12 | Sanofi-Aventis Deutschland GmbH | Kombination von einem insulin und einem glp-1-agonisten |
| SI2498802T1 (sl) | 2009-11-13 | 2015-05-29 | Sanofi-Aventis Deutschland Gmbh | Farmacevtski sestavek, ki vsebuje agonist GLP-1, inzulin in metionin |
| EP3345593B1 (de) | 2009-11-13 | 2023-09-06 | Sanofi-Aventis Deutschland GmbH | Pharmazeutische zusammensetzung umfassend despro36exendin-4(1-39)-lys6-nh2 und methionin |
| US20140148384A1 (en) | 2010-08-30 | 2014-05-29 | Sanofi-Aventis Deutschland Gmbh | Use of ave0010 for the manufacture of a medicament for the treatment of diabetes mellitus type 2 |
| US9821032B2 (en) | 2011-05-13 | 2017-11-21 | Sanofi-Aventis Deutschland Gmbh | Pharmaceutical combination for improving glycemic control as add-on therapy to basal insulin |
| CN103917241A (zh) | 2011-08-29 | 2014-07-09 | 赛诺菲-安万特德国有限公司 | 用于2型糖尿病患者中的血糖控制的药物组合 |
| TWI559929B (en) | 2011-09-01 | 2016-12-01 | Sanofi Aventis Deutschland | Pharmaceutical composition for use in the treatment of a neurodegenerative disease |
| LT3229828T (lt) | 2014-12-12 | 2023-06-12 | Sanofi-Aventis Deutschland Gmbh | Insulino glargino/liksisenatido fiksuoto santykio kompozicija |
| TWI748945B (zh) | 2015-03-13 | 2021-12-11 | 德商賽諾菲阿凡提斯德意志有限公司 | 第２型糖尿病病患治療 |
| TW201705975A (zh) | 2015-03-18 | 2017-02-16 | 賽諾菲阿凡提斯德意志有限公司 | 第２型糖尿病病患之治療 |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SE337223B (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) * | 1967-05-23 | 1971-08-02 | Pharmacia Ab | |
| US3935072A (en) * | 1972-08-10 | 1976-01-27 | Tanabe Seiyaku Co., Ltd. | Purification of coenzyme A |
| US3876501A (en) * | 1973-05-17 | 1975-04-08 | Baxter Laboratories Inc | Binding enzymes to activated water-soluble carbohydrates |
| US3919049A (en) * | 1974-06-27 | 1975-11-11 | Tokyo Tanabe Co | Process for preparing {62 -galactosidase |
| SE8302758L (sv) * | 1983-05-17 | 1984-11-18 | Pharmacia Ab | Sett for uppspjelkning av disulfidbindningar och derigenom erhallen forening |
| GB8317697D0 (en) * | 1983-06-29 | 1983-08-03 | Shell Int Research | Dissolution of peptides in non-aqueous and mixed non-aqueous/aqueous solvents |
| US4496689A (en) * | 1983-12-27 | 1985-01-29 | Miles Laboratories, Inc. | Covalently attached complex of alpha-1-proteinase inhibitor with a water soluble polymer |
-
1984
- 1984-11-09 DE DE19843440988 patent/DE3440988A1/de not_active Withdrawn
-
1985
- 1985-10-31 AT AT85113857T patent/ATE71109T1/de not_active IP Right Cessation
- 1985-10-31 EP EP85113857A patent/EP0180920B1/de not_active Expired - Lifetime
- 1985-10-31 DE DE8585113857T patent/DE3585078D1/de not_active Expired - Fee Related
- 1985-11-07 PT PT81445A patent/PT81445B/pt not_active IP Right Cessation
- 1985-11-07 ES ES548617A patent/ES8701780A1/es not_active Expired
- 1985-11-07 FI FI854383A patent/FI85494C/fi not_active IP Right Cessation
- 1985-11-07 GR GR852693A patent/GR852693B/el unknown
- 1985-11-07 US US06/795,920 patent/US4644057A/en not_active Expired - Lifetime
- 1985-11-08 IE IE279585A patent/IE58683B1/en not_active IP Right Cessation
- 1985-11-08 AU AU49713/85A patent/AU580348B2/en not_active Ceased
- 1985-11-08 DK DK516485A patent/DK166546C/da not_active IP Right Cessation
- 1985-11-08 CA CA000494971A patent/CA1247081A/en not_active Expired
- 1985-11-08 ZA ZA858603A patent/ZA858603B/xx unknown
- 1985-11-08 JP JP60249148A patent/JPH0714960B2/ja not_active Expired - Lifetime
- 1985-11-09 KR KR1019850008387A patent/KR930007428B1/ko not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| LT3328B (en) | 1990-09-05 | 1995-07-25 | Hoechst Ag | Process for the preparation of insulin |
| US5728543A (en) * | 1990-09-05 | 1998-03-17 | Hoechst Aktiengesellschaft | Clostripain catalyzed hydrolysis of preproinsulin analogs into corresponding insulins |
| WO2010016069A1 (en) | 2008-08-07 | 2010-02-11 | Biocon Limited | A process for preparation of insulin compounds |
Also Published As
| Publication number | Publication date |
|---|---|
| AU580348B2 (en) | 1989-01-12 |
| IE852795L (en) | 1986-05-09 |
| ZA858603B (en) | 1986-07-30 |
| DK516485D0 (da) | 1985-11-08 |
| FI854383A0 (fi) | 1985-11-07 |
| DK166546B (da) | 1993-06-07 |
| DE3585078D1 (de) | 1992-02-13 |
| FI85494B (fi) | 1992-01-15 |
| US4644057A (en) | 1987-02-17 |
| FI85494C (fi) | 1992-04-27 |
| ES548617A0 (es) | 1986-12-01 |
| JPS61115096A (ja) | 1986-06-02 |
| PT81445A (de) | 1985-12-01 |
| ES8701780A1 (es) | 1986-12-01 |
| KR860003832A (ko) | 1986-06-13 |
| EP0180920A3 (en) | 1989-01-04 |
| DK516485A (da) | 1986-05-10 |
| AU4971385A (en) | 1986-05-15 |
| GR852693B (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) | 1986-03-10 |
| KR930007428B1 (ko) | 1993-08-10 |
| CA1247081A (en) | 1988-12-20 |
| DK166546C (da) | 1993-10-25 |
| JPH0714960B2 (ja) | 1995-02-22 |
| EP0180920B1 (de) | 1992-01-02 |
| EP0180920A2 (de) | 1986-05-14 |
| ATE71109T1 (de) | 1992-01-15 |
| PT81445B (pt) | 1987-12-30 |
| IE58683B1 (en) | 1993-11-03 |
| FI854383L (fi) | 1986-05-10 |
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