DE3301102C2 - - Google Patents
Info
- Publication number
- DE3301102C2 DE3301102C2 DE3301102A DE3301102A DE3301102C2 DE 3301102 C2 DE3301102 C2 DE 3301102C2 DE 3301102 A DE3301102 A DE 3301102A DE 3301102 A DE3301102 A DE 3301102A DE 3301102 C2 DE3301102 C2 DE 3301102C2
- Authority
- DE
- Germany
- Prior art keywords
- cells
- immobilization
- particles
- water
- cell
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 210000004027 cell Anatomy 0.000 claims description 145
- 239000002245 particle Substances 0.000 claims description 84
- 239000000243 solution Substances 0.000 claims description 71
- 239000000463 material Substances 0.000 claims description 57
- 239000000725 suspension Substances 0.000 claims description 54
- 102000004190 Enzymes Human genes 0.000 claims description 46
- 108090000790 Enzymes Proteins 0.000 claims description 46
- 210000001822 immobilized cell Anatomy 0.000 claims description 41
- 238000000034 method Methods 0.000 claims description 39
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 39
- 229920013730 reactive polymer Polymers 0.000 claims description 37
- 238000003756 stirring Methods 0.000 claims description 36
- 238000004519 manufacturing process Methods 0.000 claims description 24
- 239000011541 reaction mixture Substances 0.000 claims description 21
- SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical compound O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 claims description 18
- 239000000126 substance Substances 0.000 claims description 16
- 239000011942 biocatalyst Substances 0.000 claims description 15
- 239000000203 mixture Substances 0.000 claims description 14
- 229920002873 Polyethylenimine Polymers 0.000 claims description 12
- 108010039918 Polylysine Proteins 0.000 claims description 9
- 229920000656 polylysine Polymers 0.000 claims description 9
- 125000000467 secondary amino group Chemical group [H]N([*:1])[*:2] 0.000 claims description 9
- 239000004094 surface-active agent Substances 0.000 claims description 8
- 239000004472 Lysine Substances 0.000 claims description 7
- 230000001133 acceleration Effects 0.000 claims description 7
- 239000000853 adhesive Substances 0.000 claims description 7
- 230000001070 adhesive effect Effects 0.000 claims description 7
- 238000001914 filtration Methods 0.000 claims description 7
- 239000012634 fragment Substances 0.000 claims description 7
- 239000003960 organic solvent Substances 0.000 claims description 7
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 claims description 7
- 239000012736 aqueous medium Substances 0.000 claims description 6
- NAQMVNRVTILPCV-UHFFFAOYSA-N hexane-1,6-diamine Chemical compound NCCCCCCN NAQMVNRVTILPCV-UHFFFAOYSA-N 0.000 claims description 6
- 230000008823 permeabilization Effects 0.000 claims description 6
- 230000008569 process Effects 0.000 claims description 6
- 239000007853 buffer solution Substances 0.000 claims description 4
- 230000018044 dehydration Effects 0.000 claims description 4
- 238000006297 dehydration reaction Methods 0.000 claims description 4
- 230000003100 immobilizing effect Effects 0.000 claims description 4
- ZNZYKNKBJPZETN-WELNAUFTSA-N Dialdehyde 11678 Chemical compound N1C2=CC=CC=C2C2=C1[C@H](C[C@H](/C(=C/O)C(=O)OC)[C@@H](C=C)C=O)NCC2 ZNZYKNKBJPZETN-WELNAUFTSA-N 0.000 claims description 3
- 239000002253 acid Substances 0.000 claims description 3
- 238000000926 separation method Methods 0.000 claims description 3
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 claims description 2
- 150000007513 acids Chemical class 0.000 claims description 2
- 150000003839 salts Chemical class 0.000 claims description 2
- 239000002904 solvent Substances 0.000 claims description 2
- 238000004065 wastewater treatment Methods 0.000 claims description 2
- 238000007711 solidification Methods 0.000 claims 3
- 230000008023 solidification Effects 0.000 claims 3
- 230000000694 effects Effects 0.000 description 62
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 54
- 229940088598 enzyme Drugs 0.000 description 31
- 238000004062 sedimentation Methods 0.000 description 22
- 238000006243 chemical reaction Methods 0.000 description 18
- 229930006000 Sucrose Natural products 0.000 description 13
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 13
- 239000005720 sucrose Substances 0.000 description 13
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 12
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 12
- 239000008103 glucose Substances 0.000 description 12
- 241000196324 Embryophyta Species 0.000 description 11
- KDXKERNSBIXSRK-YFKPBYRVSA-N L-lysine Chemical compound NCCCC[C@H](N)C(O)=O KDXKERNSBIXSRK-YFKPBYRVSA-N 0.000 description 11
- 239000000758 substrate Substances 0.000 description 11
- 238000007710 freezing Methods 0.000 description 10
- 230000008014 freezing Effects 0.000 description 10
- 239000006228 supernatant Substances 0.000 description 10
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 9
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 9
- 230000002255 enzymatic effect Effects 0.000 description 9
- 239000008363 phosphate buffer Substances 0.000 description 9
- CKLJMWTZIZZHCS-UHFFFAOYSA-N D-OH-Asp Natural products OC(=O)C(N)CC(O)=O CKLJMWTZIZZHCS-UHFFFAOYSA-N 0.000 description 8
- CKLJMWTZIZZHCS-UWTATZPHSA-N L-Aspartic acid Natural products OC(=O)[C@H](N)CC(O)=O CKLJMWTZIZZHCS-UWTATZPHSA-N 0.000 description 8
- CKLJMWTZIZZHCS-REOHCLBHSA-N L-aspartic acid Chemical compound OC(=O)[C@@H](N)CC(O)=O CKLJMWTZIZZHCS-REOHCLBHSA-N 0.000 description 8
- 229930013930 alkaloid Natural products 0.000 description 8
- 229960005261 aspartic acid Drugs 0.000 description 8
- 230000001788 irregular Effects 0.000 description 8
- 238000002360 preparation method Methods 0.000 description 8
- 239000013049 sediment Substances 0.000 description 8
- 230000015572 biosynthetic process Effects 0.000 description 7
- 239000003431 cross linking reagent Substances 0.000 description 7
- 239000007788 liquid Substances 0.000 description 7
- 230000036961 partial effect Effects 0.000 description 7
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 6
- -1 Hydroxyalkyl methacrylates Chemical class 0.000 description 6
- 108010073038 Penicillin Amidase Proteins 0.000 description 6
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 description 6
- 235000002634 Solanum Nutrition 0.000 description 6
- 241000207763 Solanum Species 0.000 description 6
- 229910052782 aluminium Inorganic materials 0.000 description 6
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 6
- 238000001035 drying Methods 0.000 description 6
- 238000005189 flocculation Methods 0.000 description 6
- 230000016615 flocculation Effects 0.000 description 6
- 238000005755 formation reaction Methods 0.000 description 6
- 239000002609 medium Substances 0.000 description 6
- 238000002156 mixing Methods 0.000 description 6
- 230000035484 reaction time Effects 0.000 description 6
- 239000011780 sodium chloride Substances 0.000 description 6
- 238000005406 washing Methods 0.000 description 6
- 241000588724 Escherichia coli Species 0.000 description 5
- 235000019766 L-Lysine Nutrition 0.000 description 5
- 230000001580 bacterial effect Effects 0.000 description 5
- 239000000969 carrier Substances 0.000 description 5
- 238000000855 fermentation Methods 0.000 description 5
- 230000004151 fermentation Effects 0.000 description 5
- 229930182830 galactose Natural products 0.000 description 5
- 238000005259 measurement Methods 0.000 description 5
- 239000008399 tap water Substances 0.000 description 5
- 235000020679 tap water Nutrition 0.000 description 5
- 241000894006 Bacteria Species 0.000 description 4
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 4
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 4
- 241000233866 Fungi Species 0.000 description 4
- 239000004698 Polyethylene Substances 0.000 description 4
- 239000002262 Schiff base Substances 0.000 description 4
- 150000004753 Schiff bases Chemical class 0.000 description 4
- 239000007864 aqueous solution Substances 0.000 description 4
- 210000003527 eukaryotic cell Anatomy 0.000 description 4
- 239000008394 flocculating agent Substances 0.000 description 4
- 239000011888 foil Substances 0.000 description 4
- 239000008187 granular material Substances 0.000 description 4
- 244000005700 microbiome Species 0.000 description 4
- 229920000573 polyethylene Polymers 0.000 description 4
- 229920000642 polymer Polymers 0.000 description 4
- 210000001236 prokaryotic cell Anatomy 0.000 description 4
- 239000004753 textile Substances 0.000 description 4
- 229920003169 water-soluble polymer Polymers 0.000 description 4
- 102000004031 Carboxy-Lyases Human genes 0.000 description 3
- 108090000489 Carboxy-Lyases Proteins 0.000 description 3
- 229930091371 Fructose Natural products 0.000 description 3
- 239000005715 Fructose Substances 0.000 description 3
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 description 3
- ZMXDDKWLCZADIW-UHFFFAOYSA-N N,N-Dimethylformamide Chemical compound CN(C)C=O ZMXDDKWLCZADIW-UHFFFAOYSA-N 0.000 description 3
- 229910000831 Steel Inorganic materials 0.000 description 3
- 230000004913 activation Effects 0.000 description 3
- 125000003172 aldehyde group Chemical group 0.000 description 3
- 108010051210 beta-Fructofuranosidase Proteins 0.000 description 3
- 102000005936 beta-Galactosidase Human genes 0.000 description 3
- 108010005774 beta-Galactosidase Proteins 0.000 description 3
- 239000000872 buffer Substances 0.000 description 3
- 238000005119 centrifugation Methods 0.000 description 3
- 239000003153 chemical reaction reagent Substances 0.000 description 3
- 238000010908 decantation Methods 0.000 description 3
- 239000011521 glass Substances 0.000 description 3
- 238000000265 homogenisation Methods 0.000 description 3
- 229910052751 metal Inorganic materials 0.000 description 3
- 239000002184 metal Substances 0.000 description 3
- 230000000813 microbial effect Effects 0.000 description 3
- 229940068065 phytosterols Drugs 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- 239000007787 solid Substances 0.000 description 3
- 239000010959 steel Substances 0.000 description 3
- 230000008961 swelling Effects 0.000 description 3
- 241000186361 Actinobacteria <class> Species 0.000 description 2
- 239000001729 Ammonium fumarate Substances 0.000 description 2
- 241000193830 Bacillus <bacterium> Species 0.000 description 2
- 102000004157 Hydrolases Human genes 0.000 description 2
- 108090000604 Hydrolases Proteins 0.000 description 2
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 2
- DCXYFEDJOCDNAF-REOHCLBHSA-N L-asparagine Chemical compound OC(=O)[C@@H](N)CC(N)=O DCXYFEDJOCDNAF-REOHCLBHSA-N 0.000 description 2
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 description 2
- STNJBCKSHOAVAJ-UHFFFAOYSA-N Methacrolein Chemical compound CC(=C)C=O STNJBCKSHOAVAJ-UHFFFAOYSA-N 0.000 description 2
- 239000007983 Tris buffer Substances 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
- 239000008351 acetate buffer Substances 0.000 description 2
- 230000009471 action Effects 0.000 description 2
- 235000019297 ammonium fumarate Nutrition 0.000 description 2
- CKKXWJDFFQPBQL-SEPHDYHBSA-N azane;(e)-but-2-enedioic acid Chemical compound N.N.OC(=O)\C=C\C(O)=O CKKXWJDFFQPBQL-SEPHDYHBSA-N 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 238000005842 biochemical reaction Methods 0.000 description 2
- 230000036983 biotransformation Effects 0.000 description 2
- 238000009835 boiling Methods 0.000 description 2
- 238000007444 cell Immobilization Methods 0.000 description 2
- 239000006285 cell suspension Substances 0.000 description 2
- 230000001413 cellular effect Effects 0.000 description 2
- 238000007385 chemical modification Methods 0.000 description 2
- 238000004587 chromatography analysis Methods 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 238000007596 consolidation process Methods 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
- 238000009792 diffusion process Methods 0.000 description 2
- 238000006911 enzymatic reaction Methods 0.000 description 2
- 239000001963 growth medium Substances 0.000 description 2
- 238000004128 high performance liquid chromatography Methods 0.000 description 2
- 238000002347 injection Methods 0.000 description 2
- 239000007924 injection Substances 0.000 description 2
- 239000001573 invertase Substances 0.000 description 2
- 235000011073 invertase Nutrition 0.000 description 2
- 239000008101 lactose Substances 0.000 description 2
- 229920005615 natural polymer Polymers 0.000 description 2
- 230000003071 parasitic effect Effects 0.000 description 2
- 238000006116 polymerization reaction Methods 0.000 description 2
- 238000003825 pressing Methods 0.000 description 2
- OVARTBFNCCXQKS-UHFFFAOYSA-N propan-2-one;hydrate Chemical compound O.CC(C)=O OVARTBFNCCXQKS-UHFFFAOYSA-N 0.000 description 2
- 230000002797 proteolythic effect Effects 0.000 description 2
- 230000001105 regulatory effect Effects 0.000 description 2
- 238000004626 scanning electron microscopy Methods 0.000 description 2
- 239000011343 solid material Substances 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 238000012546 transfer Methods 0.000 description 2
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 2
- 239000002699 waste material Substances 0.000 description 2
- RYHBNJHYFVUHQT-UHFFFAOYSA-N 1,4-Dioxane Chemical compound C1COCCO1 RYHBNJHYFVUHQT-UHFFFAOYSA-N 0.000 description 1
- HRPVXLWXLXDGHG-UHFFFAOYSA-N Acrylamide Chemical compound NC(=O)C=C HRPVXLWXLXDGHG-UHFFFAOYSA-N 0.000 description 1
- 229920001817 Agar Polymers 0.000 description 1
- 102100032948 Aspartoacylase Human genes 0.000 description 1
- 241000228245 Aspergillus niger Species 0.000 description 1
- 208000035404 Autolysis Diseases 0.000 description 1
- 239000002028 Biomass Substances 0.000 description 1
- DKPFZGUDAPQIHT-UHFFFAOYSA-N Butyl acetate Natural products CCCCOC(C)=O DKPFZGUDAPQIHT-UHFFFAOYSA-N 0.000 description 1
- 102100035882 Catalase Human genes 0.000 description 1
- 108010053835 Catalase Proteins 0.000 description 1
- 206010057248 Cell death Diseases 0.000 description 1
- 229920002284 Cellulose triacetate Polymers 0.000 description 1
- 229920002101 Chitin Polymers 0.000 description 1
- 229920001661 Chitosan Polymers 0.000 description 1
- 102000008186 Collagen Human genes 0.000 description 1
- 108010035532 Collagen Proteins 0.000 description 1
- 241000289669 Erinaceus europaeus Species 0.000 description 1
- 241000588698 Erwinia Species 0.000 description 1
- 108010093031 Galactosidases Proteins 0.000 description 1
- 102000002464 Galactosidases Human genes 0.000 description 1
- 108010010803 Gelatin Proteins 0.000 description 1
- 108010015776 Glucose oxidase Proteins 0.000 description 1
- 101000797251 Homo sapiens Aspartoacylase Proteins 0.000 description 1
- 108090000769 Isomerases Proteins 0.000 description 1
- 102000004195 Isomerases Human genes 0.000 description 1
- 244000285963 Kluyveromyces fragilis Species 0.000 description 1
- 235000014663 Kluyveromyces fragilis Nutrition 0.000 description 1
- 102000004895 Lipoproteins Human genes 0.000 description 1
- 108090001030 Lipoproteins Proteins 0.000 description 1
- 102000004317 Lyases Human genes 0.000 description 1
- 108090000856 Lyases Proteins 0.000 description 1
- 108010014251 Muramidase Proteins 0.000 description 1
- 102000016943 Muramidase Human genes 0.000 description 1
- 241000187488 Mycobacterium sp. Species 0.000 description 1
- 108010062010 N-Acetylmuramoyl-L-alanine Amidase Proteins 0.000 description 1
- 102000004316 Oxidoreductases Human genes 0.000 description 1
- 108090000854 Oxidoreductases Proteins 0.000 description 1
- 241000222051 Papiliotrema laurentii Species 0.000 description 1
- 239000004952 Polyamide Substances 0.000 description 1
- 240000007776 Solanum aviculare Species 0.000 description 1
- 235000002591 Solanum aviculare Nutrition 0.000 description 1
- 241000719745 Streptomyces phaechromogenes Species 0.000 description 1
- RTAQQCXQSZGOHL-UHFFFAOYSA-N Titanium Chemical compound [Ti] RTAQQCXQSZGOHL-UHFFFAOYSA-N 0.000 description 1
- 238000005411 Van der Waals force Methods 0.000 description 1
- 108700040099 Xylose isomerases Proteins 0.000 description 1
- QCWXUUIWCKQGHC-UHFFFAOYSA-N Zirconium Chemical compound [Zr] QCWXUUIWCKQGHC-UHFFFAOYSA-N 0.000 description 1
- NNLVGZFZQQXQNW-ADJNRHBOSA-N [(2r,3r,4s,5r,6s)-4,5-diacetyloxy-3-[(2s,3r,4s,5r,6r)-3,4,5-triacetyloxy-6-(acetyloxymethyl)oxan-2-yl]oxy-6-[(2r,3r,4s,5r,6s)-4,5,6-triacetyloxy-2-(acetyloxymethyl)oxan-3-yl]oxyoxan-2-yl]methyl acetate Chemical compound O([C@@H]1O[C@@H]([C@H]([C@H](OC(C)=O)[C@H]1OC(C)=O)O[C@H]1[C@@H]([C@@H](OC(C)=O)[C@H](OC(C)=O)[C@@H](COC(C)=O)O1)OC(C)=O)COC(=O)C)[C@@H]1[C@@H](COC(C)=O)O[C@@H](OC(C)=O)[C@H](OC(C)=O)[C@H]1OC(C)=O NNLVGZFZQQXQNW-ADJNRHBOSA-N 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 150000003797 alkaloid derivatives Chemical class 0.000 description 1
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 1
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 1
- 235000011130 ammonium sulphate Nutrition 0.000 description 1
- 230000003698 anagen phase Effects 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 229960001230 asparagine Drugs 0.000 description 1
- 108010041102 azocasein Proteins 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
- IYNDLOXRXUOGIU-LQDWTQKMSA-M benzylpenicillin potassium Chemical compound [K+].N([C@H]1[C@H]2SC([C@@H](N2C1=O)C([O-])=O)(C)C)C(=O)CC1=CC=CC=C1 IYNDLOXRXUOGIU-LQDWTQKMSA-M 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 230000001588 bifunctional effect Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 210000002421 cell wall Anatomy 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 238000012512 characterization method Methods 0.000 description 1
- 238000001311 chemical methods and process Methods 0.000 description 1
- 229920001436 collagen Polymers 0.000 description 1
- 238000010924 continuous production Methods 0.000 description 1
- 238000007334 copolymerization reaction Methods 0.000 description 1
- 230000009089 cytolysis Effects 0.000 description 1
- 210000000805 cytoplasm Anatomy 0.000 description 1
- 230000003413 degradative effect Effects 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 239000002158 endotoxin Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 229960003133 ergot alkaloid Drugs 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 238000011049 filling Methods 0.000 description 1
- 239000012065 filter cake Substances 0.000 description 1
- 238000011010 flushing procedure Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 239000000499 gel Substances 0.000 description 1
- 239000008273 gelatin Substances 0.000 description 1
- 229920000159 gelatin Polymers 0.000 description 1
- 235000019322 gelatine Nutrition 0.000 description 1
- 235000011852 gelatine desserts Nutrition 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- VOZRXNHHFUQHIL-UHFFFAOYSA-N glycidyl methacrylate Chemical compound CC(=C)C(=O)OCC1CO1 VOZRXNHHFUQHIL-UHFFFAOYSA-N 0.000 description 1
- 230000034659 glycolysis Effects 0.000 description 1
- 238000000227 grinding Methods 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- FUZZWVXGSFPDMH-UHFFFAOYSA-N hexanoic acid Chemical compound CCCCCC(O)=O FUZZWVXGSFPDMH-UHFFFAOYSA-N 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 230000002779 inactivation Effects 0.000 description 1
- 229910010272 inorganic material Inorganic materials 0.000 description 1
- 239000011147 inorganic material Substances 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 239000000543 intermediate Substances 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 210000003093 intracellular space Anatomy 0.000 description 1
- 230000002427 irreversible effect Effects 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 229920006008 lipopolysaccharide Polymers 0.000 description 1
- 235000018977 lysine Nutrition 0.000 description 1
- 229960000274 lysozyme Drugs 0.000 description 1
- 239000004325 lysozyme Substances 0.000 description 1
- 235000010335 lysozyme Nutrition 0.000 description 1
- 230000002101 lytic effect Effects 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 238000000386 microscopy Methods 0.000 description 1
- ZIUHHBKFKCYYJD-UHFFFAOYSA-N n,n'-methylenebisacrylamide Chemical compound C=CC(=O)NCNC(=O)C=C ZIUHHBKFKCYYJD-UHFFFAOYSA-N 0.000 description 1
- 230000006855 networking Effects 0.000 description 1
- 239000011368 organic material Substances 0.000 description 1
- 230000035515 penetration Effects 0.000 description 1
- 210000001322 periplasm Anatomy 0.000 description 1
- 239000003208 petroleum Substances 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 229920002647 polyamide Polymers 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 230000036632 reaction speed Effects 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 238000012552 review Methods 0.000 description 1
- 230000033764 rhythmic process Effects 0.000 description 1
- 230000028043 self proteolysis Effects 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 238000005029 sieve analysis Methods 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 230000003595 spectral effect Effects 0.000 description 1
- 230000002269 spontaneous effect Effects 0.000 description 1
- 229910001220 stainless steel Inorganic materials 0.000 description 1
- 239000010935 stainless steel Substances 0.000 description 1
- 239000007858 starting material Substances 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 229920001059 synthetic polymer Polymers 0.000 description 1
- 238000005496 tempering Methods 0.000 description 1
- 238000010257 thawing Methods 0.000 description 1
- 230000036962 time dependent Effects 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 229910052719 titanium Inorganic materials 0.000 description 1
- 239000010936 titanium Substances 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- GPPXJZIENCGNKB-UHFFFAOYSA-N vanadium Chemical compound [V]#[V] GPPXJZIENCGNKB-UHFFFAOYSA-N 0.000 description 1
- 229910052720 vanadium Inorganic materials 0.000 description 1
- 210000005253 yeast cell Anatomy 0.000 description 1
- 229910052726 zirconium Inorganic materials 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
- C12N11/08—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
- C12N11/089—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds
Landscapes
- Zoology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CS82277A CS231458B1 (en) | 1982-01-14 | 1982-01-14 | Cellular catalysts for biotransformation and method to produce them |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DE3301102A1 DE3301102A1 (de) | 1983-07-21 |
| DE3301102C2 true DE3301102C2 (en:Method) | 1987-09-17 |
Family
ID=5334616
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE19833301102 Granted DE3301102A1 (de) | 1982-01-14 | 1983-01-14 | Cellulaere biokatalysatoren und ihre herstellung |
Country Status (6)
| Country | Link |
|---|---|
| JP (1) | JPS58155092A (en:Method) |
| AT (1) | AT388933B (en:Method) |
| CS (1) | CS231458B1 (en:Method) |
| DE (1) | DE3301102A1 (en:Method) |
| IN (1) | IN155662B (en:Method) |
| YU (1) | YU44732B (en:Method) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3301992A1 (de) * | 1983-01-21 | 1984-07-26 | Boehringer Ingelheim KG, 6507 Ingelheim | Permeabilisiertes schimmelpilzmycel mit intaktem immobilisiertem glucoseoxidase-katalasesystem sowie dessen herstellung und anwendung |
| US4600692A (en) * | 1983-02-10 | 1986-07-15 | Purification Engineering, Inc. | Immobilized cells for preparing phenylalanine |
| CA1210717A (en) * | 1983-08-10 | 1986-09-02 | Oreste J. Lantero, Jr. | Immobilization of biocatalysts |
| KR20030076721A (ko) * | 1997-09-09 | 2003-09-26 | 바이오케미 게젤샤프트 엠베하 | 무 에스테라제 효소 |
| AT409380B (de) * | 1997-09-09 | 2002-07-25 | Biochemie Gmbh | Verfahren zur herstellung von biokatalysatoren |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5617073B2 (en:Method) * | 1971-10-28 | 1981-04-20 | ||
| GB1444539A (en) * | 1972-09-11 | 1976-08-04 | Novo Industri As | Immobilised enzymes |
| SU659611A1 (ru) * | 1977-02-09 | 1979-04-30 | Московский государственный университет им. М.В.Ломоносова | Способ получени -аспарагиновой кислоты |
| US4212943A (en) * | 1978-03-27 | 1980-07-15 | Miles Laboratories, Inc. | Production of bacterial cell aggregate |
| DK146942C (da) * | 1978-04-19 | 1984-07-30 | Novo Industri As | Fremgangsmaade til fremstilling af et immobiliseret enzymprodukt |
| US4251632A (en) * | 1978-09-11 | 1981-02-17 | Miles Laboratories, Inc. | Preparation of a bacterial cell aggregate |
-
1982
- 1982-01-14 CS CS82277A patent/CS231458B1/cs unknown
-
1983
- 1983-01-05 AT AT0002783A patent/AT388933B/de not_active IP Right Cessation
- 1983-01-07 IN IN31/CAL/83A patent/IN155662B/en unknown
- 1983-01-13 YU YU70/83A patent/YU44732B/xx unknown
- 1983-01-14 JP JP58003581A patent/JPS58155092A/ja active Granted
- 1983-01-14 DE DE19833301102 patent/DE3301102A1/de active Granted
Also Published As
| Publication number | Publication date |
|---|---|
| IN155662B (en:Method) | 1985-02-23 |
| CS231458B1 (en) | 1984-11-19 |
| YU44732B (en) | 1991-02-28 |
| ATA2783A (de) | 1989-02-15 |
| JPH0325159B2 (en:Method) | 1991-04-05 |
| JPS58155092A (ja) | 1983-09-14 |
| DE3301102A1 (de) | 1983-07-21 |
| AT388933B (de) | 1989-09-25 |
| YU7083A (en) | 1985-10-31 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| DE2721829C3 (de) | Verfahren zur Herstellung eines immobilisierte mikrobielle Zellen enthaltenden hydrophilen komplexen Xerogels | |
| DE2915135C2 (en:Method) | ||
| DE69032263T2 (de) | Biomosaische Polymere und Verfahren zu deren Herstellung | |
| DE2537993C2 (de) | Neues Glukose-Isomerase-Produkt und Verfahren zu seiner Herstellung | |
| DE3520001C2 (de) | Verfahren zur Herstellung immobilisierter Mikroorganismen oder Enzyme | |
| DE1915970A1 (de) | Verfahren zur Herstellung von Produkten auf der Basis aktiver Proteinsubstanzen | |
| DE2265121B2 (de) | Enzympräparat zur Bestimmung von Cholesterin und Verfahren zu seiner Herstellung | |
| Ionescu et al. | Amperometric algal Chlorella vulgaris cell biosensors based on alginate and polypyrrole‐alginate gels | |
| DE2407961C3 (de) | Enzymatisch aktive Membran, Verfahren zu ihrer Herstellung und ihre Verwendung | |
| DE2560532C2 (en:Method) | ||
| DE2232645C3 (de) | Verfahren zur Durchführung einer enzymkatalysierten Umwandlung eines Substrats | |
| DE3414083C2 (en:Method) | ||
| DE2930859C2 (de) | Verfahren zur Herstellung von immobilisierten Enzymen oder Mikroorganismen | |
| DE69705023T2 (de) | Enzymatische gelierung von polymermaterialien | |
| DE3301102C2 (en:Method) | ||
| US5739015A (en) | Biotransformation of chitin to chitosan | |
| US4954443A (en) | Method of immobilizing biochemically active substance with xanthan gum | |
| DE2242710A1 (de) | Komplex eines biologisch aktiven proteins mit einem polymeren | |
| DE2413694C3 (en:Method) | ||
| DD294729A5 (de) | Verfahren zur herstellung von immobilisaten mit biologisch aktiven, makromolekularen verbindungen | |
| DE2950985A1 (de) | Gebundene zellen mit penicillinacylaseaktivitaet, ihre herstellung und verwendung | |
| DE3854472T2 (de) | Erhaltung der Viabilität von Mikroorganismen zur Anwendung in mikrobiellen Inokulantien. | |
| DE69120319T2 (de) | Durch Einschliessen immobilisierte Biokatalysatoren und Verfahren zu ihrer Herstellung | |
| DE2809777C3 (de) | Verfahren zur Herstellung eines Polysaccharide | |
| DD285370A5 (de) | Verfahren zur immobilisierung von mikroorganismen bzw. coimmobilisierung von mikroorganismen und enzymen |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 8110 | Request for examination paragraph 44 | ||
| D2 | Grant after examination | ||
| 8364 | No opposition during term of opposition | ||
| 8339 | Ceased/non-payment of the annual fee |