DE3211314A1 - Verfahren zur ermittlung von kennwerten und dispersionen, suspensionen und emulsionen - Google Patents
Verfahren zur ermittlung von kennwerten und dispersionen, suspensionen und emulsionenInfo
- Publication number
- DE3211314A1 DE3211314A1 DE19823211314 DE3211314A DE3211314A1 DE 3211314 A1 DE3211314 A1 DE 3211314A1 DE 19823211314 DE19823211314 DE 19823211314 DE 3211314 A DE3211314 A DE 3211314A DE 3211314 A1 DE3211314 A1 DE 3211314A1
- Authority
- DE
- Germany
- Prior art keywords
- histogram
- suspensions
- preparation
- brightness
- cells
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000000725 suspension Substances 0.000 title claims abstract description 69
- 238000000034 method Methods 0.000 title claims abstract description 54
- 239000000839 emulsion Substances 0.000 title claims abstract description 9
- 239000006185 dispersion Substances 0.000 title abstract description 4
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims abstract description 63
- 238000002360 preparation method Methods 0.000 claims abstract description 32
- 239000011159 matrix material Substances 0.000 claims abstract description 11
- 239000006285 cell suspension Substances 0.000 claims abstract description 9
- 238000005375 photometry Methods 0.000 claims abstract description 8
- 239000002245 particle Substances 0.000 claims abstract description 5
- 239000007962 solid dispersion Substances 0.000 claims abstract description 5
- 239000000463 material Substances 0.000 claims abstract description 3
- 150000002632 lipids Chemical class 0.000 claims description 43
- 238000000354 decomposition reaction Methods 0.000 claims description 5
- 239000002356 single layer Substances 0.000 claims description 5
- 239000010410 layer Substances 0.000 claims description 4
- 230000015556 catabolic process Effects 0.000 claims 1
- 239000010409 thin film Substances 0.000 abstract 1
- 210000004027 cell Anatomy 0.000 description 99
- 239000012188 paraffin wax Substances 0.000 description 29
- 235000015097 nutrients Nutrition 0.000 description 7
- 239000002609 medium Substances 0.000 description 6
- 238000004458 analytical method Methods 0.000 description 5
- 239000001963 growth medium Substances 0.000 description 5
- 239000007788 liquid Substances 0.000 description 5
- 238000004519 manufacturing process Methods 0.000 description 5
- 210000005253 yeast cell Anatomy 0.000 description 5
- 239000000243 solution Substances 0.000 description 4
- RBTBFTRPCNLSDE-UHFFFAOYSA-N 3,7-bis(dimethylamino)phenothiazin-5-ium Chemical compound C1=CC(N(C)C)=CC2=[S+]C3=CC(N(C)C)=CC=C3N=C21 RBTBFTRPCNLSDE-UHFFFAOYSA-N 0.000 description 3
- 239000011521 glass Substances 0.000 description 3
- 229960000907 methylthioninium chloride Drugs 0.000 description 3
- 230000002906 microbiologic effect Effects 0.000 description 3
- 239000007787 solid Substances 0.000 description 3
- 230000015572 biosynthetic process Effects 0.000 description 2
- 238000005266 casting Methods 0.000 description 2
- 230000004069 differentiation Effects 0.000 description 2
- 238000000855 fermentation Methods 0.000 description 2
- 230000004151 fermentation Effects 0.000 description 2
- 230000003834 intracellular effect Effects 0.000 description 2
- 238000011835 investigation Methods 0.000 description 2
- 238000009630 liquid culture Methods 0.000 description 2
- 238000004020 luminiscence type Methods 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 238000010186 staining Methods 0.000 description 2
- 230000000007 visual effect Effects 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 241000222120 Candida <Saccharomycetales> Species 0.000 description 1
- YDIKCZBMBPOGFT-PWUSVEHZSA-N Malvidin 3-galactoside Chemical compound [Cl-].COC1=C(O)C(OC)=CC(C=2C(=CC=3C(O)=CC(O)=CC=3[O+]=2)O[C@H]2[C@@H]([C@@H](O)[C@@H](O)[C@@H](CO)O2)O)=C1 YDIKCZBMBPOGFT-PWUSVEHZSA-N 0.000 description 1
- PXUQTDZNOHRWLI-QOPOCTTISA-O Primulin Natural products O(C)c1c(O)c(OC)cc(-c2c(O[C@H]3[C@H](O)[C@@H](O)[C@@H](O)[C@H](CO)O3)cc3c(O)cc(O)cc3[o+]2)c1 PXUQTDZNOHRWLI-QOPOCTTISA-O 0.000 description 1
- CKUAXEQHGKSLHN-UHFFFAOYSA-N [C].[N] Chemical compound [C].[N] CKUAXEQHGKSLHN-UHFFFAOYSA-N 0.000 description 1
- DPKHZNPWBDQZCN-UHFFFAOYSA-N acridine orange free base Chemical compound C1=CC(N(C)C)=CC2=NC3=CC(N(C)C)=CC=C3C=C21 DPKHZNPWBDQZCN-UHFFFAOYSA-N 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- DZBUGLKDJFMEHC-UHFFFAOYSA-N benzoquinolinylidene Natural products C1=CC=CC2=CC3=CC=CC=C3N=C21 DZBUGLKDJFMEHC-UHFFFAOYSA-N 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 210000002421 cell wall Anatomy 0.000 description 1
- 238000005520 cutting process Methods 0.000 description 1
- 210000000805 cytoplasm Anatomy 0.000 description 1
- 230000006735 deficit Effects 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 238000003113 dilution method Methods 0.000 description 1
- BNIILDVGGAEEIG-UHFFFAOYSA-L disodium hydrogen phosphate Chemical compound [Na+].[Na+].OP([O-])([O-])=O BNIILDVGGAEEIG-UHFFFAOYSA-L 0.000 description 1
- 229910000397 disodium phosphate Inorganic materials 0.000 description 1
- 235000019800 disodium phosphate Nutrition 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 229930195733 hydrocarbon Natural products 0.000 description 1
- 150000002430 hydrocarbons Chemical class 0.000 description 1
- 238000007654 immersion Methods 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 239000000155 melt Substances 0.000 description 1
- 238000000386 microscopy Methods 0.000 description 1
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 1
- 235000019796 monopotassium phosphate Nutrition 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 230000001590 oxidative effect Effects 0.000 description 1
- 238000002135 phase contrast microscopy Methods 0.000 description 1
- 239000008363 phosphate buffer Substances 0.000 description 1
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 238000004886 process control Methods 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 230000005855 radiation Effects 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000009897 systematic effect Effects 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 238000004448 titration Methods 0.000 description 1
- 238000002255 vaccination Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/1468—Optical investigation techniques, e.g. flow cytometry with spatial resolution of the texture or inner structure of the particle
Landscapes
- Chemical & Material Sciences (AREA)
- Dispersion Chemistry (AREA)
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE19823211314 DE3211314A1 (de) | 1982-03-26 | 1982-03-26 | Verfahren zur ermittlung von kennwerten und dispersionen, suspensionen und emulsionen |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE19823211314 DE3211314A1 (de) | 1982-03-26 | 1982-03-26 | Verfahren zur ermittlung von kennwerten und dispersionen, suspensionen und emulsionen |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DE3211314A1 true DE3211314A1 (de) | 1983-09-29 |
| DE3211314C2 DE3211314C2 (ja) | 1987-04-23 |
Family
ID=6159461
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE19823211314 Granted DE3211314A1 (de) | 1982-03-26 | 1982-03-26 | Verfahren zur ermittlung von kennwerten und dispersionen, suspensionen und emulsionen |
Country Status (1)
| Country | Link |
|---|---|
| DE (1) | DE3211314A1 (ja) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0468705A2 (en) * | 1990-07-25 | 1992-01-29 | Hitachi, Ltd. | Method and apparatus for investigating and controlling an object |
| WO1992016614A1 (en) * | 1991-03-21 | 1992-10-01 | University College London | Improvements in, or relating to, biomass and differentiation measurements on microbial organisms |
| GB2578299A (en) * | 2018-10-19 | 2020-05-06 | Institute Of Tech Sligo | Systems for controlling an emulsification process |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US2449586A (en) * | 1942-08-15 | 1948-09-21 | Borg Warner | Transmission and control therefor |
| DE2261949A1 (de) * | 1971-12-20 | 1973-07-05 | Miles Lab | Vorrichtung zum messen und anzeigen eines abgetasteten merkmals |
| DE2537343B2 (de) * | 1974-08-29 | 1980-04-03 | Shimadzu Seisakusho Ltd., Kyoto (Japan) | Verfahren zur densitometrischen Messung einer auf einem Trägermaterial entwickelten Substanzzone und Densitometer zur Durchführung des Verfahrens |
| US4205384A (en) * | 1977-09-14 | 1980-05-27 | Wibau Gmbh | Method for the analytic determination of physical characteristics of a material |
-
1982
- 1982-03-26 DE DE19823211314 patent/DE3211314A1/de active Granted
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US2449586A (en) * | 1942-08-15 | 1948-09-21 | Borg Warner | Transmission and control therefor |
| DE2261949A1 (de) * | 1971-12-20 | 1973-07-05 | Miles Lab | Vorrichtung zum messen und anzeigen eines abgetasteten merkmals |
| DE2537343B2 (de) * | 1974-08-29 | 1980-04-03 | Shimadzu Seisakusho Ltd., Kyoto (Japan) | Verfahren zur densitometrischen Messung einer auf einem Trägermaterial entwickelten Substanzzone und Densitometer zur Durchführung des Verfahrens |
| US4205384A (en) * | 1977-09-14 | 1980-05-27 | Wibau Gmbh | Method for the analytic determination of physical characteristics of a material |
Non-Patent Citations (2)
| Title |
|---|
| DD-Prospekt der Fa. Ernst Leitz GmbH, D 6330 Wetzlar, Liste 521-275, VII/71/Lx/SD, Leitz-Classimat * |
| DE-Z.: Technisches Messen, tm, 1979, H. 7/8, S. 267-270 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0468705A2 (en) * | 1990-07-25 | 1992-01-29 | Hitachi, Ltd. | Method and apparatus for investigating and controlling an object |
| EP0468705A3 (en) * | 1990-07-25 | 1992-04-15 | Hitachi, Ltd. | Method and apparatus for investigating and controlling an object |
| US5403735A (en) * | 1990-07-25 | 1995-04-04 | Hitachi, Ltd. | Method and apparatus for investigating and controlling an object |
| WO1992016614A1 (en) * | 1991-03-21 | 1992-10-01 | University College London | Improvements in, or relating to, biomass and differentiation measurements on microbial organisms |
| GB2578299A (en) * | 2018-10-19 | 2020-05-06 | Institute Of Tech Sligo | Systems for controlling an emulsification process |
Also Published As
| Publication number | Publication date |
|---|---|
| DE3211314C2 (ja) | 1987-04-23 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| OM8 | Search report available as to paragraph 43 lit. 1 sentence 1 patent law | ||
| 8110 | Request for examination paragraph 44 | ||
| D2 | Grant after examination | ||
| 8364 | No opposition during term of opposition | ||
| 8339 | Ceased/non-payment of the annual fee |