DE3128411A1 - Kulturstamm-mutante von esccherichia coli sowie verfahren zur herstellung von phenylalanin - Google Patents
Kulturstamm-mutante von esccherichia coli sowie verfahren zur herstellung von phenylalaninInfo
- Publication number
- DE3128411A1 DE3128411A1 DE19813128411 DE3128411A DE3128411A1 DE 3128411 A1 DE3128411 A1 DE 3128411A1 DE 19813128411 DE19813128411 DE 19813128411 DE 3128411 A DE3128411 A DE 3128411A DE 3128411 A1 DE3128411 A1 DE 3128411A1
- Authority
- DE
- Germany
- Prior art keywords
- microorganism
- phenylalanine
- enzyme
- culture
- formation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Ceased
Links
- COLNVLDHVKWLRT-QMMMGPOBSA-N L-phenylalanine Chemical compound OC(=O)[C@@H](N)CC1=CC=CC=C1 COLNVLDHVKWLRT-QMMMGPOBSA-N 0.000 title claims description 46
- COLNVLDHVKWLRT-UHFFFAOYSA-N phenylalanine Natural products OC(=O)C(N)CC1=CC=CC=C1 COLNVLDHVKWLRT-UHFFFAOYSA-N 0.000 title claims description 42
- 238000004519 manufacturing process Methods 0.000 title claims description 28
- 238000000034 method Methods 0.000 title claims description 15
- 244000005700 microbiome Species 0.000 claims description 42
- 108090000790 Enzymes Proteins 0.000 claims description 25
- 102000004190 Enzymes Human genes 0.000 claims description 25
- 108010080376 3-Deoxy-7-Phosphoheptulonate Synthase Proteins 0.000 claims description 23
- 230000015572 biosynthetic process Effects 0.000 claims description 22
- 241000588724 Escherichia coli Species 0.000 claims description 20
- 108090000623 proteins and genes Proteins 0.000 claims description 20
- WTFXTQVDAKGDEY-UHFFFAOYSA-N (-)-chorismic acid Natural products OC1C=CC(C(O)=O)=CC1OC(=C)C(O)=O WTFXTQVDAKGDEY-UHFFFAOYSA-N 0.000 claims description 16
- WTFXTQVDAKGDEY-HTQZYQBOSA-N chorismic acid Chemical compound O[C@@H]1C=CC(C(O)=O)=C[C@H]1OC(=C)C(O)=O WTFXTQVDAKGDEY-HTQZYQBOSA-N 0.000 claims description 16
- 230000002068 genetic effect Effects 0.000 claims description 15
- 230000005764 inhibitory process Effects 0.000 claims description 15
- OUYCCCASQSFEME-QMMMGPOBSA-N L-tyrosine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-QMMMGPOBSA-N 0.000 claims description 11
- 239000013612 plasmid Substances 0.000 claims description 10
- PJWIPEXIFFQAQZ-PUFIMZNGSA-N 7-phospho-2-dehydro-3-deoxy-D-arabino-heptonic acid Chemical compound OP(=O)(O)OC[C@@H](O)[C@@H](O)[C@H](O)CC(=O)C(O)=O PJWIPEXIFFQAQZ-PUFIMZNGSA-N 0.000 claims description 9
- 239000001963 growth medium Substances 0.000 claims description 9
- 239000002609 medium Substances 0.000 claims description 8
- OUYCCCASQSFEME-UHFFFAOYSA-N tyrosine Natural products OC(=O)C(N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-UHFFFAOYSA-N 0.000 claims description 8
- 102000004867 Hydro-Lyases Human genes 0.000 claims description 7
- 108090001042 Hydro-Lyases Proteins 0.000 claims description 7
- 108020001482 shikimate kinase Proteins 0.000 claims description 7
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims description 6
- QIVBCDIJIAJPQS-VIFPVBQESA-N L-tryptophane Chemical compound C1=CC=C2C(C[C@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-VIFPVBQESA-N 0.000 claims description 6
- QIVBCDIJIAJPQS-UHFFFAOYSA-N Tryptophan Natural products C1=CC=C2C(CC(N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-UHFFFAOYSA-N 0.000 claims description 6
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 5
- 239000008103 glucose Substances 0.000 claims description 5
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 4
- SRBFZHDQGSBBOR-IOVATXLUSA-N D-xylopyranose Chemical compound O[C@@H]1COC(O)[C@H](O)[C@H]1O SRBFZHDQGSBBOR-IOVATXLUSA-N 0.000 claims description 4
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims description 4
- 239000002028 Biomass Substances 0.000 claims description 3
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 3
- 150000001720 carbohydrates Chemical class 0.000 claims description 3
- 229910052799 carbon Inorganic materials 0.000 claims description 3
- 238000010790 dilution Methods 0.000 claims description 3
- 239000012895 dilution Substances 0.000 claims description 3
- 230000012010 growth Effects 0.000 claims description 3
- 230000037353 metabolic pathway Effects 0.000 claims description 3
- OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 claims description 2
- GUBGYTABKSRVRQ-XLOQQCSPSA-N Alpha-Lactose Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)O[C@H](O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-XLOQQCSPSA-N 0.000 claims description 2
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 claims description 2
- GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 claims description 2
- PYMYPHUHKUWMLA-UHFFFAOYSA-N arabinose Natural products OCC(O)C(O)C(O)C=O PYMYPHUHKUWMLA-UHFFFAOYSA-N 0.000 claims description 2
- SRBFZHDQGSBBOR-UHFFFAOYSA-N beta-D-Pyranose-Lyxose Natural products OC1COC(O)C(O)C1O SRBFZHDQGSBBOR-UHFFFAOYSA-N 0.000 claims description 2
- GUBGYTABKSRVRQ-QUYVBRFLSA-N beta-maltose Chemical compound OC[C@H]1O[C@H](O[C@H]2[C@H](O)[C@@H](O)[C@H](O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@@H]1O GUBGYTABKSRVRQ-QUYVBRFLSA-N 0.000 claims description 2
- 239000007795 chemical reaction product Substances 0.000 claims description 2
- 235000011187 glycerol Nutrition 0.000 claims description 2
- 150000003893 lactate salts Chemical class 0.000 claims description 2
- 239000008101 lactose Substances 0.000 claims description 2
- 229910052757 nitrogen Inorganic materials 0.000 claims description 2
- 108700009582 Pyruvate Dehydrogenase Acetyl-Transferring Kinase Proteins 0.000 claims 2
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 claims 1
- 101150044161 tyrR gene Proteins 0.000 description 8
- 101100259583 Bacillus subtilis (strain 168) tyrS2 gene Proteins 0.000 description 7
- 101150019536 aroF gene Proteins 0.000 description 7
- 230000035772 mutation Effects 0.000 description 7
- 239000000243 solution Substances 0.000 description 7
- 239000011573 trace mineral Substances 0.000 description 7
- 235000013619 trace mineral Nutrition 0.000 description 7
- 101100435903 Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / BCRC 11384 / JCM 1318 / LMG 3730 / NCIMB 10025) aroG gene Proteins 0.000 description 6
- 101150042732 aroC gene Proteins 0.000 description 6
- 238000006243 chemical reaction Methods 0.000 description 6
- 101150023849 pheA gene Proteins 0.000 description 6
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 4
- 229910052760 oxygen Inorganic materials 0.000 description 4
- 239000001301 oxygen Substances 0.000 description 4
- NGHMDNPXVRFFGS-IUYQGCFVSA-N D-erythrose 4-phosphate Chemical compound O=C[C@H](O)[C@H](O)COP(O)(O)=O NGHMDNPXVRFFGS-IUYQGCFVSA-N 0.000 description 3
- 108010044467 Isoenzymes Proteins 0.000 description 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 229930029653 phosphoenolpyruvate Natural products 0.000 description 3
- DTBNBXWJWCWCIK-UHFFFAOYSA-N phosphoenolpyruvic acid Chemical compound OC(=O)C(=C)OP(O)(O)=O DTBNBXWJWCWCIK-UHFFFAOYSA-N 0.000 description 3
- JXOHGGNKMLTUBP-HSUXUTPPSA-N shikimic acid Chemical compound O[C@@H]1CC(C(O)=O)=C[C@@H](O)[C@H]1O JXOHGGNKMLTUBP-HSUXUTPPSA-N 0.000 description 3
- JXOHGGNKMLTUBP-JKUQZMGJSA-N shikimic acid Natural products O[C@@H]1CC(C(O)=O)=C[C@H](O)[C@@H]1O JXOHGGNKMLTUBP-JKUQZMGJSA-N 0.000 description 3
- 101150083154 tyrA gene Proteins 0.000 description 3
- KRKNYBCHXYNGOX-UHFFFAOYSA-K Citrate Chemical compound [O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O KRKNYBCHXYNGOX-UHFFFAOYSA-K 0.000 description 2
- 101100408135 Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) phnA gene Proteins 0.000 description 2
- 101100002724 Thermus thermophilus aroH gene Proteins 0.000 description 2
- 150000001413 amino acids Chemical class 0.000 description 2
- 101150076125 aroG gene Proteins 0.000 description 2
- 239000000543 intermediate Substances 0.000 description 2
- 239000013067 intermediate product Substances 0.000 description 2
- 230000037361 pathway Effects 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 239000000758 substrate Substances 0.000 description 2
- 230000001629 suppression Effects 0.000 description 2
- 238000003786 synthesis reaction Methods 0.000 description 2
- 101150044170 trpE gene Proteins 0.000 description 2
- 101000644386 Brevibacillus parabrevis Phenylalanine racemase [ATP-hydrolyzing] Proteins 0.000 description 1
- 101000906861 Chondromyces crocatus ATP-dependent tyrosine adenylase Proteins 0.000 description 1
- 108010000898 Chorismate mutase Proteins 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- 229930003451 Vitamin B1 Natural products 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- -1 aromatic amino acids Chemical class 0.000 description 1
- 125000003118 aryl group Chemical group 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 230000006696 biosynthetic metabolic pathway Effects 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
- 210000000349 chromosome Anatomy 0.000 description 1
- 230000021615 conjugation Effects 0.000 description 1
- 230000001276 controlling effect Effects 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 102000034356 gene-regulatory proteins Human genes 0.000 description 1
- 108091006104 gene-regulatory proteins Proteins 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 235000010755 mineral Nutrition 0.000 description 1
- 238000002703 mutagenesis Methods 0.000 description 1
- 231100000350 mutagenesis Toxicity 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 238000012552 review Methods 0.000 description 1
- 238000009666 routine test Methods 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- QYOJSKGCWNAKGW-HCWXCVPCSA-N shikimate-3-phosphate Chemical compound O[C@H]1CC(C(O)=O)=C[C@H](OP(O)(O)=O)[C@@H]1O QYOJSKGCWNAKGW-HCWXCVPCSA-N 0.000 description 1
- 230000002226 simultaneous effect Effects 0.000 description 1
- 239000001509 sodium citrate Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 230000002194 synthesizing effect Effects 0.000 description 1
- 229960003495 thiamine Drugs 0.000 description 1
- DPJRMOMPQZCRJU-UHFFFAOYSA-M thiamine hydrochloride Chemical compound Cl.[Cl-].CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N DPJRMOMPQZCRJU-UHFFFAOYSA-M 0.000 description 1
- 230000026683 transduction Effects 0.000 description 1
- 238000010361 transduction Methods 0.000 description 1
- HRXKRNGNAMMEHJ-UHFFFAOYSA-K trisodium citrate Chemical compound [Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O HRXKRNGNAMMEHJ-UHFFFAOYSA-K 0.000 description 1
- 229940038773 trisodium citrate Drugs 0.000 description 1
- 241001515965 unidentified phage Species 0.000 description 1
- 239000011691 vitamin B1 Substances 0.000 description 1
- 235000010374 vitamin B1 Nutrition 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P13/00—Preparation of nitrogen-containing organic compounds
- C12P13/04—Alpha- or beta- amino acids
- C12P13/22—Tryptophan; Tyrosine; Phenylalanine; 3,4-Dihydroxyphenylalanine
- C12P13/222—Phenylalanine
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/8215—Microorganisms
- Y10S435/822—Microorganisms using bacteria or actinomycetales
- Y10S435/848—Escherichia
- Y10S435/849—Escherichia coli
Landscapes
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| AU459080 | 1980-07-18 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DE3128411A1 true DE3128411A1 (de) | 1983-09-15 |
Family
ID=3695035
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE19813128411 Ceased DE3128411A1 (de) | 1980-07-18 | 1981-07-17 | Kulturstamm-mutante von esccherichia coli sowie verfahren zur herstellung von phenylalanin |
Country Status (4)
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0140606A1 (en) * | 1983-10-07 | 1985-05-08 | Biotechnica International, Inc. | Cells and their use in methods for producing product compounds |
| EP0145156A1 (en) * | 1983-10-07 | 1985-06-19 | Biotechnica International, Inc. | Expression vectors, host cells comprising such vectors and methods of making desired compounds using such cells |
Families Citing this family (54)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5169768A (en) * | 1983-10-07 | 1992-12-08 | Biotechnica International, Inc. | Method of biosynthesis of phenylalanine |
| GB8333797D0 (en) * | 1983-12-19 | 1984-01-25 | Searle & Co | Starch utilization |
| JPS6178378A (ja) * | 1984-09-27 | 1986-04-21 | Ajinomoto Co Inc | 芳香族アミノ酸生合成遺伝子を含有する組換えｄｎａ及びそれを有するコリネ型細菌 |
| EP0190921A3 (en) * | 1985-02-04 | 1988-01-13 | Engenics, Inc. | Method for the overproduction of amino acids |
| JPS63500215A (ja) * | 1985-06-24 | 1988-01-28 | ザ ヌトラスウイ−ト カンパニ− | アミノ酸合成用の複合プラスミド |
| US4753883A (en) * | 1986-05-07 | 1988-06-28 | Biotechnica International, Inc. | Enzyme deregulation |
| KR890003714B1 (ko) * | 1987-03-26 | 1989-09-30 | 주식회사 미원 | 유전자 조작 미생물에 의한 l-페닐알라닌 제조방법 |
| KR890003682B1 (ko) * | 1987-03-26 | 1989-09-30 | 주식회사 미원 | 유전자조작 미생물에 의한 l-페닐알라닌의 제조방법 |
| US5100775A (en) * | 1988-03-16 | 1992-03-31 | Smyczek Peter J | Method for conducting nucleic acid hybridization in chamber with precise fluid delivery |
| US5019509A (en) * | 1988-04-20 | 1991-05-28 | Genetics Institute, Inc. | Method and compositions for the production of l-alanine and derivatives thereof |
| US5543052A (en) * | 1988-07-28 | 1996-08-06 | Alliedsignal Inc. | Process for removal of organic pollutants from waste water |
| US5580770A (en) * | 1989-11-02 | 1996-12-03 | Alliedsignal Inc. | Support containing particulate adsorbent and microorganisms for removal of pollutants |
| US4957686A (en) * | 1990-02-06 | 1990-09-18 | Norris Alan H | Use of bacteriophages to inhibit dental caries |
| DE69133587D1 (de) * | 1990-11-30 | 2008-01-03 | Ajinomoto Kk | Rekombinante DNS-Sequenzen kodierend für Enzyme frei von Feedback-Inhibition, Plasmide, die diese Sequenzen enthalten, transformierte Mikroorganismen, nützlich für Produktion aromatischer Aminosäuren, und deren Verfahren zur Herstellung durch Fermentation |
| US5168056A (en) * | 1991-02-08 | 1992-12-01 | Purdue Research Foundation | Enhanced production of common aromatic pathway compounds |
| DE69126141T2 (de) * | 1991-08-26 | 1997-08-28 | Kyowa Hakko Kogyo Kk | Verfahren zur herstellung von 4-hydroxy-l-prolin |
| US5409830A (en) * | 1992-12-16 | 1995-04-25 | Miwon Co. Ltd. | Method for production of l-phenylalanine by Echerichia coli mutant that is resistant to osmotic pressure |
| US5776736A (en) * | 1992-12-21 | 1998-07-07 | Purdue Research Foundation | Deblocking the common pathway of aromatic amino acid synthesis |
| KR100359131B1 (ko) * | 1992-12-21 | 2003-03-06 | 제넨코 인터내셔날 인코포레이티드 | 방향족아미노산합성의경로의탈차단방법 |
| US5395527A (en) * | 1993-07-01 | 1995-03-07 | Eco Equipement Fep Inc. | Process and apparatus for treating wastewater in a dynamic, bio sequenced manner |
| US5487987A (en) * | 1993-09-16 | 1996-01-30 | Purdue Research Foundation | Synthesis of adipic acid from biomass-derived carbon sources |
| US5629181A (en) * | 1993-09-16 | 1997-05-13 | Purdue Research Foundation | Synthesis of catechol from biomass-derived carbon sources |
| US5487986A (en) * | 1994-03-10 | 1996-01-30 | Genzyme Corporation | Method for increasing plasmid yield |
| US5614392A (en) * | 1995-06-01 | 1997-03-25 | Food Industry Research And Development Institute | Process of L-phenylalanine fermentation |
| US6274369B1 (en) | 1996-02-02 | 2001-08-14 | Invitrogen Corporation | Method capable of increasing competency of bacterial cell transformation |
| EP0921814A4 (en) | 1996-03-29 | 2001-10-04 | Life Technologies Inc | Process for increasing the viability and transformation efficiency of bacteria during storage at low temperatures |
| AUPO258196A0 (en) * | 1996-09-27 | 1996-10-17 | Australian National University, The | Method for determining ion channel activity of a substance |
| EP1005529B1 (en) | 1997-02-12 | 2005-04-27 | Invitrogen Corporation | Methods for lyophilizing competent cells |
| US6210937B1 (en) | 1997-04-22 | 2001-04-03 | Bechtel Bwxt Idaho, Llc | Development of genetically engineered bacteria for production of selected aromatic compounds |
| US6030819A (en) * | 1998-09-28 | 2000-02-29 | General Electric Company | Genetically engineered microorganisms and method for producing 4-hydroxybenzoic acid |
| EP1187930B1 (en) * | 1999-05-28 | 2006-08-02 | The University Of North Texas Health Science Center At Fort Worth | A global regulator used to enhance the synthesis of aromatic substances |
| RU2202607C2 (ru) * | 2000-03-03 | 2003-04-20 | Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" | Штамм бактерий methylophilus methylotrophus - продуцент l-фенилаланина (варианты), способ получения l-фенилаланина |
| WO2003024211A2 (en) * | 2001-09-14 | 2003-03-27 | Invitrogen Corporation | Composition for stabilizing biological materials |
| KR100608085B1 (ko) * | 2004-02-05 | 2006-08-02 | 씨제이 주식회사 | ｔｙｒＲ 유전자가 불활성화된 Ｌ-쓰레오닌 생성 미생물,그를 제조하는 방법 및 상기 미생물을 이용한Ｌ-쓰레오닌의 제조방법 |
| US7482140B2 (en) * | 2004-06-15 | 2009-01-27 | Ajinomoto Co., Inc. | L-tyrosine-producing bacterium and a method for producing L-tyrosine |
| JP4665613B2 (ja) * | 2004-06-15 | 2011-04-06 | 味の素株式会社 | Ｌ−チロシン生産菌及びｌ−チロシンの製造法 |
| US20060035310A1 (en) * | 2004-08-13 | 2006-02-16 | Millipore Corporation | Media for recovery of microorganism in the presence of antibiotics |
| WO2006124895A2 (en) * | 2005-05-16 | 2006-11-23 | Massachusetts Institute Of Technology | Mutations for enhanced tyrosine production |
| US7582474B2 (en) * | 2005-07-11 | 2009-09-01 | Honeywell International Inc. | Process reactor with layered packed bed |
| EP1764415A1 (en) * | 2005-09-20 | 2007-03-21 | Nederlandse Organisatie voor Toegepast-Natuuurwetenschappelijk Onderzoek TNO | Microbial production of p-hydroxybenzyl alcohol |
| US7700328B2 (en) * | 2006-06-07 | 2010-04-20 | E.I. Du Pont De Nemours And Company | Method for producing an L-tyrosine over-producing bacterial strain |
| RU2006129690A (ru) | 2006-08-16 | 2008-02-27 | Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" (ЗАО АГРИ) (RU) | СПОСОБ ПОЛУЧЕНИЯ L-АМИНОКИСЛОТЫ С ИСПОЛЬЗОВАНИЕМ БАКТЕРИЙ СЕМЕЙСТВА Enterobacteriaceae, В КОТОРОЙ ОСЛАБЛЕНА ЭКСПРЕССИЯ ГЕНА ydiN, ГЕНА ydiB ИЛИ ИХ КОМБИНАЦИИ |
| US20080102499A1 (en) * | 2006-10-27 | 2008-05-01 | Lori Jean Templeton | Method of enhancing L-tyrosine production in recombinant bacteria |
| EP2674490B1 (en) | 2011-01-31 | 2017-04-19 | Asahi Kasei Kabushiki Kaisha | Phenylpyruvate reductase and method for manufacturing optically-active phenyllactic acid and 4-hydroxyl-phenyllactic acid using same enzyme |
| WO2012122333A1 (en) | 2011-03-08 | 2012-09-13 | Arizona Board Of Regents, A Body Corporate Of The State Of Arizona, Acting For And On Behalf Of Arizona State University | Microbial conversion of glucose to styrene and its derivatives |
| EP2809771B1 (en) | 2012-01-30 | 2017-12-27 | Myriant Corporation | Production of muconic acid from genetically engineered microorganisms |
| WO2015031048A1 (en) | 2013-08-27 | 2015-03-05 | Arizina Board Of Regents On Behalf Of Arizona State University | Microorganisms engineered to produce phenol and its derivatives |
| BR122023023164A2 (pt) * | 2015-05-27 | 2024-03-05 | Lanzatech Nz, Inc. | Bactéria geneticamente modificada fixadora de c1 capaz de produzir pelo menos um produto derivado de corismato, e, método de fabricação de um produto de fermentação |
| EP3423563B1 (en) | 2016-03-02 | 2023-05-24 | PTT Global Chemical Public Company Limited | Improved muconic acid production from genetically engineered microorganisms |
| CN110494560A (zh) | 2017-02-06 | 2019-11-22 | 齐默尔根公司 | 通过发酵产生酪胺的经改造的生物合成途径 |
| US11708590B2 (en) | 2017-07-18 | 2023-07-25 | Arizona Board Of Regents On Behalf Of Arizona State University | Synthetic metabolic funneling for biochemical production |
| US11613768B2 (en) | 2017-07-25 | 2023-03-28 | Arizona Board Of Regents On Behalf Of Arizona State University | Microbial production of 2-phenylethanol from renewable substrates |
| WO2019194279A1 (en) * | 2018-04-06 | 2019-10-10 | Ajinomoto Co., Inc. | AGRICULTURAL OR HORTICULTURAL COMPOSITION CONTAINING CULTURE BROTH OF Bacillus BACTERIUM |
| CN112080452B (zh) * | 2020-08-17 | 2023-04-28 | 天津科技大学 | 一种高产苯乳酸地衣芽孢杆菌基因工程菌、生产苯乳酸的方法和应用 |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US2973304A (en) * | 1958-10-02 | 1961-02-28 | Pfizer & Co C | Fermentation process |
| US3700558A (en) * | 1967-11-20 | 1972-10-24 | Lepetit Spa | Production of l-tryptophan by fermentation |
| US3660235A (en) * | 1969-08-22 | 1972-05-02 | Ajinomoto Kk | Method for producing phenylalanine by fermentation |
| SU875663A1 (ru) * | 1978-06-30 | 1982-09-15 | Всесоюзный научно-исследовательский институт генетики и селекции промышленных микроорганизмов | Штаммы е.coLI ВНИИГенетика VL 334 @ N6 и ВНИИГенетика VL 334 @ N7-продуценты L-треонина и способ их получени |
| FR2440957A1 (fr) * | 1978-11-13 | 1980-06-06 | Pasteur Institut | Vecteurs appropries a l'insertion dans leurs genomes de fragments d'adn etrangers, selon l'une quelconque des phases de traduction possibles, et moyens pour leur fabrication |
| JPS5573868A (en) * | 1978-11-22 | 1980-06-03 | Mitsubishi Electric Corp | Coating method for thin aluminum-silicon alloy film |
| JPS561890A (en) * | 1979-06-15 | 1981-01-10 | Ajinomoto Co Inc | Preparation of l-phenylalanine by fermentation |
-
1981
- 1981-07-17 DE DE19813128411 patent/DE3128411A1/de not_active Ceased
- 1981-07-17 JP JP56112061A patent/JPS57170184A/ja active Pending
- 1981-07-20 FR FR8114056A patent/FR2486961A1/fr active Granted
-
1983
- 1983-01-18 US US06/459,302 patent/US4681852A/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0140606A1 (en) * | 1983-10-07 | 1985-05-08 | Biotechnica International, Inc. | Cells and their use in methods for producing product compounds |
| EP0145156A1 (en) * | 1983-10-07 | 1985-06-19 | Biotechnica International, Inc. | Expression vectors, host cells comprising such vectors and methods of making desired compounds using such cells |
Also Published As
| Publication number | Publication date |
|---|---|
| US4681852A (en) | 1987-07-21 |
| JPS57170184A (en) | 1982-10-20 |
| FR2486961B1 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) | 1985-04-12 |
| FR2486961A1 (fr) | 1982-01-22 |
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