DE3019554A1 - Gewinnung von attenuierten viren fuer lebendimpfstoffe - Google Patents
Gewinnung von attenuierten viren fuer lebendimpfstoffeInfo
- Publication number
- DE3019554A1 DE3019554A1 DE19803019554 DE3019554A DE3019554A1 DE 3019554 A1 DE3019554 A1 DE 3019554A1 DE 19803019554 DE19803019554 DE 19803019554 DE 3019554 A DE3019554 A DE 3019554A DE 3019554 A1 DE3019554 A1 DE 3019554A1
- Authority
- DE
- Germany
- Prior art keywords
- nucleic acid
- infection
- viral nucleic
- virus
- prodn
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 241000700605 Viruses Species 0.000 title claims abstract description 30
- 150000007523 nucleic acids Chemical class 0.000 title claims abstract description 11
- 102000039446 nucleic acids Human genes 0.000 title claims abstract description 11
- 108020004707 nucleic acids Proteins 0.000 title claims abstract description 11
- 229960005486 vaccine Drugs 0.000 title claims abstract description 11
- 208000015181 infectious disease Diseases 0.000 title claims abstract description 10
- 230000002238 attenuated effect Effects 0.000 title claims abstract description 8
- 230000003612 virological effect Effects 0.000 title claims abstract 6
- 238000001890 transfection Methods 0.000 claims abstract description 8
- 238000000034 method Methods 0.000 claims abstract description 5
- 229920002307 Dextran Polymers 0.000 claims abstract description 4
- 208000000474 Poliomyelitis Diseases 0.000 claims abstract description 3
- 239000000126 substance Substances 0.000 claims abstract description 3
- 241000709664 Picornaviridae Species 0.000 claims abstract 2
- 239000000654 additive Substances 0.000 claims abstract 2
- 230000000996 additive effect Effects 0.000 claims abstract 2
- 238000004519 manufacturing process Methods 0.000 claims description 4
- 238000004113 cell culture Methods 0.000 claims description 3
- 241001465754 Metazoa Species 0.000 description 7
- 238000010790 dilution Methods 0.000 description 4
- 239000012895 dilution Substances 0.000 description 4
- 230000002458 infectious effect Effects 0.000 description 4
- 230000007918 pathogenicity Effects 0.000 description 4
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 241000709687 Coxsackievirus Species 0.000 description 2
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 2
- 241000699670 Mus sp. Species 0.000 description 2
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 2
- DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 description 2
- 230000001717 pathogenic effect Effects 0.000 description 2
- 231100000255 pathogenic effect Toxicity 0.000 description 2
- 229920002477 rna polymer Polymers 0.000 description 2
- 210000002966 serum Anatomy 0.000 description 2
- UCSJYZPVAKXKNQ-HZYVHMACSA-N streptomycin Chemical compound CN[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O[C@H]1O[C@@H]1[C@](C=O)(O)[C@H](C)O[C@H]1O[C@@H]1[C@@H](NC(N)=N)[C@H](O)[C@@H](NC(N)=N)[C@H](O)[C@H]1O UCSJYZPVAKXKNQ-HZYVHMACSA-N 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- 208000035473 Communicable disease Diseases 0.000 description 1
- 241000146321 Coxsackievirus A7 Species 0.000 description 1
- 201000005505 Measles Diseases 0.000 description 1
- 208000005647 Mumps Diseases 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- 229930182555 Penicillin Natural products 0.000 description 1
- JGSARLDLIJGVTE-MBNYWOFBSA-N Penicillin G Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-MBNYWOFBSA-N 0.000 description 1
- 235000014676 Phragmites communis Nutrition 0.000 description 1
- 208000003152 Yellow Fever Diseases 0.000 description 1
- 229940031567 attenuated vaccine Drugs 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000005587 bubbling Effects 0.000 description 1
- 244000309466 calf Species 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 230000000120 cytopathologic effect Effects 0.000 description 1
- 230000034994 death Effects 0.000 description 1
- 231100000517 death Toxicity 0.000 description 1
- 150000002170 ethers Chemical class 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 230000005847 immunogenicity Effects 0.000 description 1
- 230000036512 infertility Effects 0.000 description 1
- 208000010805 mumps infectious disease Diseases 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 229940049954 penicillin Drugs 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 201000005404 rubella Diseases 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 229920006395 saturated elastomer Polymers 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 229960005322 streptomycin Drugs 0.000 description 1
- 238000010257 thawing Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 230000003313 weakening effect Effects 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/12—Viral antigens
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/12—Viral antigens
- A61K39/125—Picornaviridae, e.g. calicivirus
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
- A61K2039/525—Virus
- A61K2039/5254—Virus avirulent or attenuated
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
- C12N2770/00011—Details
- C12N2770/32011—Picornaviridae
- C12N2770/32311—Enterovirus
- C12N2770/32334—Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
- C12N2770/00011—Details
- C12N2770/32011—Picornaviridae
- C12N2770/32311—Enterovirus
- C12N2770/32361—Methods of inactivation or attenuation
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Virology (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Medicinal Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Veterinary Medicine (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Public Health (AREA)
- Animal Behavior & Ethology (AREA)
- Engineering & Computer Science (AREA)
- Epidemiology (AREA)
- Mycology (AREA)
- Pharmacology & Pharmacy (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE19803019554 DE3019554A1 (de) | 1980-05-22 | 1980-05-22 | Gewinnung von attenuierten viren fuer lebendimpfstoffe |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE19803019554 DE3019554A1 (de) | 1980-05-22 | 1980-05-22 | Gewinnung von attenuierten viren fuer lebendimpfstoffe |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DE3019554A1 true DE3019554A1 (de) | 1981-11-26 |
| DE3019554C2 DE3019554C2 (enrdf_load_stackoverflow) | 1988-11-17 |
Family
ID=6103057
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE19803019554 Granted DE3019554A1 (de) | 1980-05-22 | 1980-05-22 | Gewinnung von attenuierten viren fuer lebendimpfstoffe |
Country Status (1)
| Country | Link |
|---|---|
| DE (1) | DE3019554A1 (enrdf_load_stackoverflow) |
-
1980
- 1980-05-22 DE DE19803019554 patent/DE3019554A1/de active Granted
Non-Patent Citations (1)
| Title |
|---|
| NICHTS-ERMITTELT * |
Also Published As
| Publication number | Publication date |
|---|---|
| DE3019554C2 (enrdf_load_stackoverflow) | 1988-11-17 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| DE69732407T2 (de) | Verfahren für die replikation von influenza in zellkultur und die bei diesem verfahren hergestellten influenza-viren | |
| DE69227400T2 (de) | Impfstoffe und Verfahren zu ihrer Herstellung | |
| DE69626022T3 (de) | Verfahren zur industriellen herstellung eines japanischen enkephalitis impfstoffes, und so erhaltener impfstoff | |
| DE2049515A1 (de) | Impfstoff gegen Virushepatitis | |
| CH629668A5 (de) | Verfahren zum reinigen von hepatitis-b-antigen. | |
| DE1617940C2 (de) | Verfahren zur Gewinnung eines für die Züchtung von Viren geeigneten Zellstammes | |
| EP0222974B1 (de) | Verfahren zur Herstellung eines Tollwutimpfstoffes und nach diesem Verfahren erhaltener Impfstoff | |
| DE19916085A1 (de) | Verfahren zur Herstellung eines antiviralen Mittels | |
| DE1924303A1 (de) | AEthylaethylenimin als Inaktivierungsmittel | |
| DE2555169C2 (enrdf_load_stackoverflow) | ||
| DE2616407A1 (de) | Tollwut-vaccine und verfahren zu ihrer herstellung | |
| DE2425997A1 (de) | Verfahren zur herstellung von abgeschwaechtem katzenfiebervakzin | |
| DE1198489B (de) | Verfahren zur Herstellung eines Impfstoffes gegen Panleucopenie | |
| DE3019554A1 (de) | Gewinnung von attenuierten viren fuer lebendimpfstoffe | |
| DE2415353C2 (enrdf_load_stackoverflow) | ||
| DE2456636A1 (de) | Influenza-vakzine und verfahren zu ihrer herstellung | |
| DD300833A7 (de) | Verfahren zur herstellung von inaktivierten influenza-vollvirusimpfstoffen | |
| DE3005495C2 (de) | Herstellung von Fragmenten von Viren mit Lipidhülle und sie enthaltende Arzneimittelzubereitungen | |
| DE68908414T2 (de) | Pharmazeutische zusammensetzungen für die reizung eines immunostimulierenden effektes. | |
| CN115251092A (zh) | 一种环保型能快速灭活细菌病毒的消毒剂及其制备方法 | |
| DE1235505B (de) | Verfahren zur Herstellung einer Vaccine gegen Staupe | |
| US2720485A (en) | Preparation of concentrated, purified ultraviolet inactivated hog cholera vaccine | |
| EP0025116A1 (de) | Verfahren zur Prüfung auf Inaktivierung von Impfstoffen und Unschädlichkeit von Blutprodukten sowie der Wirksamkeit von Chemotherapeutika bzw. Desinfektionsmitteln gegen Virushepatit und Verfahren zur Gewinnung von Virushepatitis-Antigen | |
| DE908909C (de) | Verfahren zur Herstellung und Verarbeitung pathogener Viren und der sie begleitenden Stoffe | |
| DE1037655B (de) | Verfahren zur Herstellung von Impfloesungen mit abgetoetetem infektioesem Hepatitisvirus |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 8110 | Request for examination paragraph 44 | ||
| D2 | Grant after examination | ||
| 8364 | No opposition during term of opposition | ||
| 8339 | Ceased/non-payment of the annual fee |