DE2953720C2 - Verfahren zum Nachweisen von Bakterien in flüssigen Proben - Google Patents
Verfahren zum Nachweisen von Bakterien in flüssigen ProbenInfo
- Publication number
- DE2953720C2 DE2953720C2 DE2953720A DE2953720A DE2953720C2 DE 2953720 C2 DE2953720 C2 DE 2953720C2 DE 2953720 A DE2953720 A DE 2953720A DE 2953720 A DE2953720 A DE 2953720A DE 2953720 C2 DE2953720 C2 DE 2953720C2
- Authority
- DE
- Germany
- Prior art keywords
- bacteria
- dye
- membrane
- urine
- staining
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 238000000034 method Methods 0.000 title claims description 49
- 239000007788 liquid Substances 0.000 title claims description 24
- 230000014670 detection of bacterium Effects 0.000 title description 4
- 241000894006 Bacteria Species 0.000 claims description 148
- 239000012528 membrane Substances 0.000 claims description 83
- 238000010186 staining Methods 0.000 claims description 42
- 238000005406 washing Methods 0.000 claims description 29
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 claims description 22
- 238000002360 preparation method Methods 0.000 claims description 21
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims description 18
- 239000004599 antimicrobial Substances 0.000 claims description 16
- 150000007524 organic acids Chemical class 0.000 claims description 12
- 238000004040 coloring Methods 0.000 claims description 11
- 239000003365 glass fiber Substances 0.000 claims description 7
- 150000003839 salts Chemical class 0.000 claims description 6
- 239000011148 porous material Substances 0.000 claims description 4
- 238000000151 deposition Methods 0.000 claims description 3
- 230000008021 deposition Effects 0.000 claims description 2
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 123
- 210000002700 urine Anatomy 0.000 description 79
- 239000000975 dye Substances 0.000 description 78
- 239000000523 sample Substances 0.000 description 39
- 230000001580 bacterial effect Effects 0.000 description 27
- 238000012360 testing method Methods 0.000 description 23
- 238000010790 dilution Methods 0.000 description 20
- 239000012895 dilution Substances 0.000 description 20
- 201000004538 Bacteriuria Diseases 0.000 description 18
- 230000003115 biocidal effect Effects 0.000 description 17
- 235000010633 broth Nutrition 0.000 description 17
- 239000011347 resin Substances 0.000 description 15
- 229920005989 resin Polymers 0.000 description 15
- 239000002253 acid Substances 0.000 description 14
- 210000004369 blood Anatomy 0.000 description 14
- 239000008280 blood Substances 0.000 description 14
- OARRHUQTFTUEOS-UHFFFAOYSA-N safranin Chemical compound [Cl-].C=12C=C(N)C(C)=CC2=NC2=CC(C)=C(N)C=C2[N+]=1C1=CC=CC=C1 OARRHUQTFTUEOS-UHFFFAOYSA-N 0.000 description 14
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 14
- 239000003085 diluting agent Substances 0.000 description 13
- 239000002738 chelating agent Substances 0.000 description 12
- 230000012010 growth Effects 0.000 description 12
- 239000000049 pigment Substances 0.000 description 12
- 239000012530 fluid Substances 0.000 description 11
- 241000588724 Escherichia coli Species 0.000 description 10
- 241000192125 Firmicutes Species 0.000 description 10
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 description 10
- 239000000463 material Substances 0.000 description 10
- 239000008188 pellet Substances 0.000 description 10
- 239000000981 basic dye Substances 0.000 description 9
- 238000011534 incubation Methods 0.000 description 9
- 230000035945 sensitivity Effects 0.000 description 9
- 230000000845 anti-microbial effect Effects 0.000 description 8
- 239000002244 precipitate Substances 0.000 description 8
- 239000000725 suspension Substances 0.000 description 8
- 239000004593 Epoxy Substances 0.000 description 7
- 239000003795 chemical substances by application Substances 0.000 description 7
- 238000002474 experimental method Methods 0.000 description 7
- 230000008569 process Effects 0.000 description 7
- 230000002829 reductive effect Effects 0.000 description 7
- 239000000243 solution Substances 0.000 description 7
- 229910019142 PO4 Inorganic materials 0.000 description 6
- 230000002401 inhibitory effect Effects 0.000 description 6
- 244000005700 microbiome Species 0.000 description 6
- 239000000203 mixture Substances 0.000 description 6
- 235000021317 phosphate Nutrition 0.000 description 6
- 238000004062 sedimentation Methods 0.000 description 6
- 210000002966 serum Anatomy 0.000 description 6
- 239000000057 synthetic resin Substances 0.000 description 6
- 229920003002 synthetic resin Polymers 0.000 description 6
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 5
- 230000015572 biosynthetic process Effects 0.000 description 5
- 238000005119 centrifugation Methods 0.000 description 5
- 239000012141 concentrate Substances 0.000 description 5
- 239000013068 control sample Substances 0.000 description 5
- 238000001514 detection method Methods 0.000 description 5
- 238000001914 filtration Methods 0.000 description 5
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 5
- 239000010452 phosphate Substances 0.000 description 5
- 239000001974 tryptic soy broth Substances 0.000 description 5
- 108010050327 trypticase-soy broth Proteins 0.000 description 5
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 4
- 239000004480 active ingredient Substances 0.000 description 4
- 239000003957 anion exchange resin Substances 0.000 description 4
- 238000006243 chemical reaction Methods 0.000 description 4
- 239000013078 crystal Substances 0.000 description 4
- 238000002845 discoloration Methods 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 238000011156 evaluation Methods 0.000 description 4
- 239000000706 filtrate Substances 0.000 description 4
- 239000001963 growth medium Substances 0.000 description 4
- 235000005985 organic acids Nutrition 0.000 description 4
- 150000002978 peroxides Chemical class 0.000 description 4
- 238000003860 storage Methods 0.000 description 4
- 229950003937 tolonium Drugs 0.000 description 4
- HNONEKILPDHFOL-UHFFFAOYSA-M tolonium chloride Chemical compound [Cl-].C1=C(C)C(N)=CC2=[S+]C3=CC(N(C)C)=CC=C3N=C21 HNONEKILPDHFOL-UHFFFAOYSA-M 0.000 description 4
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 3
- 102000001554 Hemoglobins Human genes 0.000 description 3
- 108010054147 Hemoglobins Proteins 0.000 description 3
- 241000589516 Pseudomonas Species 0.000 description 3
- 241000191967 Staphylococcus aureus Species 0.000 description 3
- 238000004458 analytical method Methods 0.000 description 3
- 239000003242 anti bacterial agent Substances 0.000 description 3
- 230000000721 bacterilogical effect Effects 0.000 description 3
- 210000000601 blood cell Anatomy 0.000 description 3
- 239000003153 chemical reaction reagent Substances 0.000 description 3
- 238000007796 conventional method Methods 0.000 description 3
- 238000003745 diagnosis Methods 0.000 description 3
- 230000002349 favourable effect Effects 0.000 description 3
- 239000008103 glucose Substances 0.000 description 3
- 208000015181 infectious disease Diseases 0.000 description 3
- 239000007791 liquid phase Substances 0.000 description 3
- 239000002609 medium Substances 0.000 description 3
- 238000004445 quantitative analysis Methods 0.000 description 3
- 239000007787 solid Substances 0.000 description 3
- 238000001179 sorption measurement Methods 0.000 description 3
- 239000011550 stock solution Substances 0.000 description 3
- 238000002560 therapeutic procedure Methods 0.000 description 3
- 208000019206 urinary tract infection Diseases 0.000 description 3
- 208000031729 Bacteremia Diseases 0.000 description 2
- 208000035143 Bacterial infection Diseases 0.000 description 2
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 2
- 201000009906 Meningitis Diseases 0.000 description 2
- 239000000020 Nitrocellulose Substances 0.000 description 2
- 239000004743 Polypropylene Substances 0.000 description 2
- 239000010425 asbestos Substances 0.000 description 2
- 208000022362 bacterial infectious disease Diseases 0.000 description 2
- 238000009640 blood culture Methods 0.000 description 2
- 210000001124 body fluid Anatomy 0.000 description 2
- 239000010839 body fluid Substances 0.000 description 2
- 238000009395 breeding Methods 0.000 description 2
- 230000001488 breeding effect Effects 0.000 description 2
- 210000004027 cell Anatomy 0.000 description 2
- 229920002301 cellulose acetate Polymers 0.000 description 2
- VUFGUVLLDPOSBC-XRZFDKQNSA-M cephalothin sodium Chemical compound [Na+].N([C@H]1[C@@H]2N(C1=O)C(=C(CS2)COC(=O)C)C([O-])=O)C(=O)CC1=CC=CS1 VUFGUVLLDPOSBC-XRZFDKQNSA-M 0.000 description 2
- 239000011248 coating agent Substances 0.000 description 2
- 238000000576 coating method Methods 0.000 description 2
- 239000003086 colorant Substances 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 230000003247 decreasing effect Effects 0.000 description 2
- 229910003460 diamond Inorganic materials 0.000 description 2
- 239000010432 diamond Substances 0.000 description 2
- 238000007865 diluting Methods 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 238000004090 dissolution Methods 0.000 description 2
- 238000004043 dyeing Methods 0.000 description 2
- 239000003822 epoxy resin Substances 0.000 description 2
- 238000000855 fermentation Methods 0.000 description 2
- 230000004151 fermentation Effects 0.000 description 2
- 239000011152 fibreglass Substances 0.000 description 2
- 239000000945 filler Substances 0.000 description 2
- 239000011521 glass Substances 0.000 description 2
- LNEPOXFFQSENCJ-UHFFFAOYSA-N haloperidol Chemical compound C1CC(O)(C=2C=CC(Cl)=CC=2)CCN1CCCC(=O)C1=CC=C(F)C=C1 LNEPOXFFQSENCJ-UHFFFAOYSA-N 0.000 description 2
- 230000002452 interceptive effect Effects 0.000 description 2
- CXKWCBBOMKCUKX-UHFFFAOYSA-M methylene blue Chemical compound [Cl-].C1=CC(N(C)C)=CC2=[S+]C3=CC(N(C)C)=CC=C3N=C21 CXKWCBBOMKCUKX-UHFFFAOYSA-M 0.000 description 2
- 229960000907 methylthioninium chloride Drugs 0.000 description 2
- 230000002906 microbiologic effect Effects 0.000 description 2
- PGSADBUBUOPOJS-UHFFFAOYSA-N neutral red Chemical compound Cl.C1=C(C)C(N)=CC2=NC3=CC(N(C)C)=CC=C3N=C21 PGSADBUBUOPOJS-UHFFFAOYSA-N 0.000 description 2
- 229920001220 nitrocellulos Polymers 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 230000001575 pathological effect Effects 0.000 description 2
- 229920000647 polyepoxide Polymers 0.000 description 2
- -1 polypropylene Polymers 0.000 description 2
- 229920001155 polypropylene Polymers 0.000 description 2
- 238000001556 precipitation Methods 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 238000004451 qualitative analysis Methods 0.000 description 2
- 230000009467 reduction Effects 0.000 description 2
- 229910052895 riebeckite Inorganic materials 0.000 description 2
- 239000013049 sediment Substances 0.000 description 2
- 239000011734 sodium Substances 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
- 239000008223 sterile water Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 239000012085 test solution Substances 0.000 description 2
- 229920001817 Agar Polymers 0.000 description 1
- 206010008796 Chromaturia Diseases 0.000 description 1
- KRKNYBCHXYNGOX-UHFFFAOYSA-K Citrate Chemical compound [O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O KRKNYBCHXYNGOX-UHFFFAOYSA-K 0.000 description 1
- CEAZRRDELHUEMR-URQXQFDESA-N Gentamicin Chemical compound O1[C@H](C(C)NC)CC[C@@H](N)[C@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](NC)[C@@](C)(O)CO2)O)[C@H](N)C[C@@H]1N CEAZRRDELHUEMR-URQXQFDESA-N 0.000 description 1
- 229930182566 Gentamicin Natural products 0.000 description 1
- 239000004471 Glycine Substances 0.000 description 1
- 238000003794 Gram staining Methods 0.000 description 1
- 238000002768 Kirby-Bauer method Methods 0.000 description 1
- 241000588748 Klebsiella Species 0.000 description 1
- NIPNSKYNPDTRPC-UHFFFAOYSA-N N-[2-oxo-2-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)ethyl]-2-[[3-(trifluoromethoxy)phenyl]methylamino]pyrimidine-5-carboxamide Chemical compound O=C(CNC(=O)C=1C=NC(=NC=1)NCC1=CC(=CC=C1)OC(F)(F)F)N1CC2=C(CC1)NN=N2 NIPNSKYNPDTRPC-UHFFFAOYSA-N 0.000 description 1
- GRYLNZFGIOXLOG-UHFFFAOYSA-N Nitric acid Chemical compound O[N+]([O-])=O GRYLNZFGIOXLOG-UHFFFAOYSA-N 0.000 description 1
- 240000007930 Oxalis acetosella Species 0.000 description 1
- 235000008098 Oxalis acetosella Nutrition 0.000 description 1
- 241000586605 Parlatoria proteus Species 0.000 description 1
- 206010034133 Pathogen resistance Diseases 0.000 description 1
- 241000588769 Proteus <enterobacteria> Species 0.000 description 1
- 241000238370 Sepia Species 0.000 description 1
- 206010040047 Sepsis Diseases 0.000 description 1
- 241000607715 Serratia marcescens Species 0.000 description 1
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 description 1
- 241000191963 Staphylococcus epidermidis Species 0.000 description 1
- 208000012931 Urologic disease Diseases 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 230000003698 anagen phase Effects 0.000 description 1
- 125000000129 anionic group Chemical group 0.000 description 1
- 238000000137 annealing Methods 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 230000003385 bacteriostatic effect Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 239000012928 buffer substance Substances 0.000 description 1
- 244000309466 calf Species 0.000 description 1
- 150000004649 carbonic acid derivatives Chemical class 0.000 description 1
- 238000005341 cation exchange Methods 0.000 description 1
- 238000005352 clarification Methods 0.000 description 1
- 230000001332 colony forming effect Effects 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 238000004042 decolorization Methods 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000035475 disorder Diseases 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 238000010828 elution Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 210000003743 erythrocyte Anatomy 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 229960002518 gentamicin Drugs 0.000 description 1
- 239000010931 gold Substances 0.000 description 1
- 229910052737 gold Inorganic materials 0.000 description 1
- 210000004884 grey matter Anatomy 0.000 description 1
- 230000001771 impaired effect Effects 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 230000002458 infectious effect Effects 0.000 description 1
- 238000001802 infusion Methods 0.000 description 1
- 239000002054 inoculum Substances 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 229910017604 nitric acid Inorganic materials 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 230000036961 partial effect Effects 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 238000005453 pelletization Methods 0.000 description 1
- 239000000825 pharmaceutical preparation Substances 0.000 description 1
- 229940127557 pharmaceutical product Drugs 0.000 description 1
- 150000003013 phosphoric acid derivatives Chemical class 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 238000012764 semi-quantitative analysis Methods 0.000 description 1
- 208000013223 septicemia Diseases 0.000 description 1
- 238000013207 serial dilution Methods 0.000 description 1
- 238000011120 smear test Methods 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 239000001632 sodium acetate Substances 0.000 description 1
- 235000017281 sodium acetate Nutrition 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 210000000278 spinal cord Anatomy 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 238000001356 surgical procedure Methods 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- UEUXEKPTXMALOB-UHFFFAOYSA-J tetrasodium;2-[2-[bis(carboxylatomethyl)amino]ethyl-(carboxylatomethyl)amino]acetate Chemical compound [Na+].[Na+].[Na+].[Na+].[O-]C(=O)CN(CC([O-])=O)CCN(CC([O-])=O)CC([O-])=O UEUXEKPTXMALOB-UHFFFAOYSA-J 0.000 description 1
- 239000012749 thinning agent Substances 0.000 description 1
- SOBHUZYZLFQYFK-UHFFFAOYSA-K trisodium;hydroxy-[[phosphonatomethyl(phosphonomethyl)amino]methyl]phosphinate Chemical class [Na+].[Na+].[Na+].OP(O)(=O)CN(CP(O)([O-])=O)CP([O-])([O-])=O SOBHUZYZLFQYFK-UHFFFAOYSA-K 0.000 description 1
- 150000007971 urates Chemical class 0.000 description 1
- 208000014001 urinary system disease Diseases 0.000 description 1
- 239000001052 yellow pigment Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- Toxicology (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US94519778A | 1978-09-25 | 1978-09-25 | |
| US06/033,900 US4225669A (en) | 1979-04-27 | 1979-04-27 | Staining and analysis of bacteria |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DE2953720C2 true DE2953720C2 (de) | 1983-10-20 |
Family
ID=26710302
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE2938511A Expired DE2938511C2 (de) | 1978-09-25 | 1979-09-24 | Präparat zum Färben von Bakterien |
| DE2953720A Expired DE2953720C2 (de) | 1978-09-25 | 1979-09-24 | Verfahren zum Nachweisen von Bakterien in flüssigen Proben |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE2938511A Expired DE2938511C2 (de) | 1978-09-25 | 1979-09-24 | Präparat zum Färben von Bakterien |
Country Status (7)
| Country | Link |
|---|---|
| BR (1) | BR7906097A (pt) |
| CA (1) | CA1112987A (pt) |
| DE (2) | DE2938511C2 (pt) |
| FR (1) | FR2436993A1 (pt) |
| GB (1) | GB2031457B (pt) |
| IT (1) | IT1164706B (pt) |
| SE (1) | SE7907581L (pt) |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4525453A (en) * | 1982-10-26 | 1985-06-25 | Eastman Kodak Company | Process for rapidly differentiating between gram-negative and gram-positive microorganisms |
| US4617264A (en) * | 1983-11-04 | 1986-10-14 | Syntex (U.S.A.) Inc. | Pretreatment method and composition |
| US4912036A (en) * | 1986-09-24 | 1990-03-27 | Eastman Kodak Company | Rapid differentiation of bacteria using cyclic polypeptide antibiotics |
| US4885239A (en) * | 1986-09-24 | 1989-12-05 | Eastman Kodak Company | Rapid differentiation of bacteria using polyether antibiotics |
| ATE110787T1 (de) * | 1987-06-12 | 1994-09-15 | Edward S Hyman | Bestimmung von bakteriuria. |
| AU2002224757A1 (en) * | 2001-01-17 | 2002-07-30 | Danish Dairy Board | Method for differential analysis of bacteria in a sample |
| CH703678B1 (de) * | 2004-04-06 | 2012-03-15 | Empa Testmaterialien Ag | Verfahren und Einrichtung zum Testen bakterizider Wirkung von Substanzen. |
| CN112574863A (zh) * | 2019-09-29 | 2021-03-30 | 广东体必康生物科技有限公司 | 一种基于双层膜过滤的细菌检测方法 |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DD101759A1 (pt) * | 1973-01-28 | 1973-11-12 |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3043751A (en) * | 1960-03-14 | 1962-07-10 | Harold C Herman | Process and equipment for determining microbial sensitivity to anti-microbial agents |
| US3554871A (en) * | 1969-05-16 | 1971-01-12 | American Sterilizer Co | Strip for staining bacteria |
| SE7408105L (pt) * | 1973-06-22 | 1974-12-23 | Univ Strathclyde | |
| GB1503828A (en) * | 1976-06-22 | 1978-03-15 | Univ Strathclyde | Method of enumerating bacteria |
-
1979
- 1979-08-23 CA CA334,321A patent/CA1112987A/en not_active Expired
- 1979-09-11 GB GB7931544A patent/GB2031457B/en not_active Expired
- 1979-09-12 SE SE7907581A patent/SE7907581L/ not_active Application Discontinuation
- 1979-09-21 IT IT50319/79A patent/IT1164706B/it active
- 1979-09-24 DE DE2938511A patent/DE2938511C2/de not_active Expired
- 1979-09-24 FR FR7923676A patent/FR2436993A1/fr active Granted
- 1979-09-24 DE DE2953720A patent/DE2953720C2/de not_active Expired
- 1979-09-24 BR BR7906097A patent/BR7906097A/pt unknown
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DD101759A1 (pt) * | 1973-01-28 | 1973-11-12 |
Non-Patent Citations (3)
| Title |
|---|
| Dickscheit, R.: Handbuch der mikro- biologischen Laboratoriumstechnik, Dresden 1967, S. 180 unter 3.2.10.5 * |
| Joergensen, A.: Mikroorganismen der Gärungsindustrie, 7. Aufl., Nürnberg 1956, S. 370 * |
| Oberzill, W.: Mirkobiologische Analytik, Nürnberg 1967, S. 95 unter 111.6, Abs. 1 u. 2 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CA1112987A (en) | 1981-11-24 |
| IT7950319A0 (it) | 1979-09-21 |
| IT1164706B (it) | 1987-04-15 |
| GB2031457B (en) | 1982-10-27 |
| BR7906097A (pt) | 1980-05-27 |
| FR2436993A1 (fr) | 1980-04-18 |
| GB2031457A (en) | 1980-04-23 |
| DE2938511A1 (de) | 1980-03-27 |
| SE7907581L (sv) | 1980-03-26 |
| DE2938511C2 (de) | 1982-09-16 |
| FR2436993B1 (pt) | 1983-04-29 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US4225669A (en) | Staining and analysis of bacteria | |
| US4336337A (en) | Detection of bacteria | |
| DE69020555T2 (de) | Fällungstest für mikroorganismen. | |
| DE68925481T2 (de) | Verfahren zum nachweis von bakterien im harn | |
| DE2823916A1 (de) | Verfahren zur selektiven bestimmung von lebensfaehigen soma- und mikroben-zellen | |
| DE1944246A1 (de) | Verfahren und Vorrichtung zur quantitativen Bestimmung von chemischen Blutsubstanzen in Blutderivaten | |
| CH639204A5 (de) | Kunststofftraeger zur durchfuehrung von analytischen oder diagnostischen untersuchungen. | |
| DE69720248T2 (de) | Schneller mikrobieller test | |
| DE3226407C2 (de) | Mikro-Analyse-Kapillar-System | |
| DE2953720C2 (de) | Verfahren zum Nachweisen von Bakterien in flüssigen Proben | |
| DE60035977T2 (de) | MIT FTA BESCHICHTETER TRäGER ZUR VERWENDUNG ALS MOLEKULARES DIAGNOSEMITTEL | |
| DE69821494T2 (de) | Anlage unf Verfahren zum Trennen von Feststoffen aus einer flüssigen Probe | |
| DE19814715A1 (de) | Gelatinemembranfilter, Verfahren zu ihrer Herstellung und ihre Verwendung | |
| DE69737718T2 (de) | Nachweis von mikroorganismen | |
| EP1573051A1 (de) | Verfahren und vorrichtung zur bestimmung von keimen | |
| DE69214473T2 (de) | Ein Verfahren zum Nachweis gramnegativer Bakterien | |
| DE2728077A1 (de) | Verfahren zum faerben von mikroorganismen | |
| DE2829316A1 (de) | Verfahren zum nachweis und zur untersuchung von zellulaerer aktivitaet und mittel zur durchfuehrung des verfahrens | |
| EP0457789B1 (de) | Verfahren zur schnellen prüfung der wirksamkeit von agenzien auf mikroorganismen | |
| DE3784702T2 (de) | Verfahren und vorrichtung zum nachweis und zur mengenbestimmung von bakterien. | |
| CH634923A5 (en) | Process for the preparation of pili from Neisseria gonorrhoeae | |
| DE69214474T2 (de) | Ein Verfahren zum Nachweis von Mikroorganismen | |
| DE3783532T2 (de) | Zusammensetzung und verfahren, die substituierte ortho-chinone als elektronen-transfermittel in analytischen bestimmungen verwenden. | |
| DE3879171T2 (de) | Verfahren und vorrichtung zur quantitativen bestimmung von mikroorganismen oder pyrogenen. | |
| DE2212573C3 (de) | Prüfmittel zur halbquantitativen Bestimmung der in einer Flüssigkeit enthaltenen Mikroorganismen |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| AC | Divided out of |
Ref country code: DE Ref document number: 2938511 Format of ref document f/p: P |
|
| Q172 | Divided out of (supplement): |
Ref country code: DE Ref document number: 2938511 |
|
| 8110 | Request for examination paragraph 44 | ||
| 8161 | Application of addition to: |
Ref document number: 2938511 Country of ref document: DE Format of ref document f/p: P |
|
| AC | Divided out of |
Ref country code: DE Ref document number: 2938511 Format of ref document f/p: P |
|
| AF | Is addition to no. |
Ref country code: DE Ref document number: 2938511 Format of ref document f/p: P |
|
| D2 | Grant after examination | ||
| 8364 | No opposition during term of opposition | ||
| 8340 | Patent of addition ceased/non-payment of fee of main patent |