GB1503828A - Method of enumerating bacteria - Google Patents

Method of enumerating bacteria

Info

Publication number
GB1503828A
GB1503828A GB2580276A GB2580276A GB1503828A GB 1503828 A GB1503828 A GB 1503828A GB 2580276 A GB2580276 A GB 2580276A GB 2580276 A GB2580276 A GB 2580276A GB 1503828 A GB1503828 A GB 1503828A
Authority
GB
United Kingdom
Prior art keywords
organisms
micro
phosphate
liquid
derivatives
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
GB2580276A
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Strathclyde
Original Assignee
University of Strathclyde
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of Strathclyde filed Critical University of Strathclyde
Priority to GB2580276A priority Critical patent/GB1503828A/en
Priority to CA280,989A priority patent/CA1089339A/en
Priority to FR7718920A priority patent/FR2355912A1/en
Priority to DE19772728077 priority patent/DE2728077A1/en
Priority to DK276677A priority patent/DK276677A/en
Priority to JP7339577A priority patent/JPS5320479A/en
Publication of GB1503828A publication Critical patent/GB1503828A/en
Expired legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2304/00—Chemical means of detecting microorganisms
    • C12Q2304/10—DNA staining

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Toxicology (AREA)
  • Biomedical Technology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

1503828 Enumerating bacteria STRATHCLYDE UNIVERSITY OF 22 June 1976 25802/76 Heading C6F A method of staining micro-organisms to facilitate counting comprises the steps of providing a suspension of micro-organisms in a liquid medium, reacting the micro-organisms in the suspended state with phosphate ions whereby phosphate ions react at active sites on the micro-organisms to produce phosphate derivatives of the micro-organisms having at said sites polydentate phosphate groups, and treating the phosphate derivatives with a phosphate-reactive fluorochrome dye (specified) which thereby combines chemically with the microorganisms via the intermediate phosphate groups. Preferably the phosphate derivatives are treated with the fluorochrome dye in the suspended state in liquid medium to produce a liquid specimen for microscopic examination in the liquid state. Alternatively, the phosphate derivatives are applied to a carrier substrate and fixed thereto using a liquid fixative, and the fixed sample is brought into contact with the fluorochrome dye to be stained, the stained sample being dried to provide on the substrate a dry specimen for microscopic examination in the dry state. The micro-organisms may be subjected to further chemical modifications e.g. methylation, esterification, hydrolysis, oxidation or reaction with sulphur dioxide, whereby to increase the number of active sites available. They may also be treated with an optical brightening agent. Many detailed Examples are provided.
GB2580276A 1976-06-22 1976-06-22 Method of enumerating bacteria Expired GB1503828A (en)

Priority Applications (6)

Application Number Priority Date Filing Date Title
GB2580276A GB1503828A (en) 1976-06-22 1976-06-22 Method of enumerating bacteria
CA280,989A CA1089339A (en) 1976-06-22 1977-06-20 Method of staining micro-organisms
FR7718920A FR2355912A1 (en) 1976-06-22 1977-06-21 MICROORGANISMS STAINING PROCESS
DE19772728077 DE2728077A1 (en) 1976-06-22 1977-06-22 METHOD OF COLORING MICRO-ORGANISMS
DK276677A DK276677A (en) 1976-06-22 1977-06-22 PROCEDURES FOR COLORING MICRO-ORGANISMS
JP7339577A JPS5320479A (en) 1976-06-22 1977-06-22 Dyeing and counting of microorganism

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB2580276A GB1503828A (en) 1976-06-22 1976-06-22 Method of enumerating bacteria

Publications (1)

Publication Number Publication Date
GB1503828A true GB1503828A (en) 1978-03-15

Family

ID=10233555

Family Applications (1)

Application Number Title Priority Date Filing Date
GB2580276A Expired GB1503828A (en) 1976-06-22 1976-06-22 Method of enumerating bacteria

Country Status (6)

Country Link
JP (1) JPS5320479A (en)
CA (1) CA1089339A (en)
DE (1) DE2728077A1 (en)
DK (1) DK276677A (en)
FR (1) FR2355912A1 (en)
GB (1) GB1503828A (en)

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1985005691A1 (en) * 1984-05-25 1985-12-19 University Of Strathclyde Apparatus for microscopic examination
WO1999060373A1 (en) * 1998-05-20 1999-11-25 Nanomont Gesellschaft Für Nanotechnologie Mbh Method and device for fixing micro- and/or nano-objects
WO2001036661A2 (en) * 1999-10-25 2001-05-25 Genprime, Inc. Method and apparatus for prokaryotic and eukaryotic cell quantitation
US6673568B1 (en) 1999-10-25 2004-01-06 Genprime, Inc. Method and apparatus for prokaryotic and eukaryotic cell quantitation
US6787302B2 (en) 1999-10-25 2004-09-07 Genprime, Inc. Method and apparatus for prokaryotic and eukaryotic cell quantitation
CN112575054A (en) * 2019-12-16 2021-03-30 中国计量科学研究院 Rapid synchronous multiple detection method and kit for total number of listeria monocytogenes and bacteria
CN113430107A (en) * 2021-06-25 2021-09-24 常州市金坛第一人民医院 Modular pretreatment system for microbial detection

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1112987A (en) * 1978-09-25 1981-11-24 Joseph L. Melnick Staining and analysis of bacteria
JPH0782009B2 (en) * 1988-03-24 1995-09-06 工業技術院長 Cell fusion method and device
DE602004010323T2 (en) 2003-03-27 2008-03-13 Nissan Chemical Industries, Ltd. Diantimony pentoxide sol and method of preparation
WO2006033283A1 (en) 2004-09-21 2006-03-30 Nissan Chemical Industries, Ltd. Process for producing antimony pentaoxide
CN103091145B (en) * 2013-01-30 2015-04-29 中国人民解放军军事医学科学院生物工程研究所 Method for staining microorganisms

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3678151A (en) * 1969-07-25 1972-07-18 Gugol Clini Tex Inc Biological staining method
SE7408105L (en) * 1973-06-22 1974-12-23 Univ Strathclyde

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1985005691A1 (en) * 1984-05-25 1985-12-19 University Of Strathclyde Apparatus for microscopic examination
WO1999060373A1 (en) * 1998-05-20 1999-11-25 Nanomont Gesellschaft Für Nanotechnologie Mbh Method and device for fixing micro- and/or nano-objects
WO2001036661A2 (en) * 1999-10-25 2001-05-25 Genprime, Inc. Method and apparatus for prokaryotic and eukaryotic cell quantitation
WO2001036661A3 (en) * 1999-10-25 2002-07-11 Genprime Inc Method and apparatus for prokaryotic and eukaryotic cell quantitation
US6673568B1 (en) 1999-10-25 2004-01-06 Genprime, Inc. Method and apparatus for prokaryotic and eukaryotic cell quantitation
US6787302B2 (en) 1999-10-25 2004-09-07 Genprime, Inc. Method and apparatus for prokaryotic and eukaryotic cell quantitation
CN112575054A (en) * 2019-12-16 2021-03-30 中国计量科学研究院 Rapid synchronous multiple detection method and kit for total number of listeria monocytogenes and bacteria
CN113430107A (en) * 2021-06-25 2021-09-24 常州市金坛第一人民医院 Modular pretreatment system for microbial detection

Also Published As

Publication number Publication date
DK276677A (en) 1977-12-23
FR2355912B1 (en) 1983-12-16
JPS5320479A (en) 1978-02-24
CA1089339A (en) 1980-11-11
DE2728077A1 (en) 1978-01-05
FR2355912A1 (en) 1978-01-20

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Legal Events

Date Code Title Description
PS Patent sealed
732 Registration of transactions, instruments or events in the register (sect. 32/1977)
732 Registration of transactions, instruments or events in the register (sect. 32/1977)
PCNP Patent ceased through non-payment of renewal fee

Effective date: 19930622