CN113430107A - Modular pretreatment system for microbial detection - Google Patents
Modular pretreatment system for microbial detection Download PDFInfo
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- CN113430107A CN113430107A CN202110713915.7A CN202110713915A CN113430107A CN 113430107 A CN113430107 A CN 113430107A CN 202110713915 A CN202110713915 A CN 202110713915A CN 113430107 A CN113430107 A CN 113430107A
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- pretreatment system
- microbial detection
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/36—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of biomass, e.g. colony counters or by turbidity measurements
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/46—Means for regulation, monitoring, measurement or control, e.g. flow regulation of cellular or enzymatic activity or functionality, e.g. cell viability
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M45/00—Means for pre-treatment of biological substances
- C12M45/02—Means for pre-treatment of biological substances by mechanical forces; Stirring; Trituration; Comminuting
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M45/00—Means for pre-treatment of biological substances
- C12M45/03—Means for pre-treatment of biological substances by control of the humidity or content of liquids; Drying
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M45/00—Means for pre-treatment of biological substances
- C12M45/04—Phase separators; Separation of non fermentable material; Fractionation
Abstract
The invention discloses a modular pretreatment system for microbial detection, which comprises a sample crushing device, a sample diluting device, a sample mixing device, a sample filtering and impurity removing device, a sample classifying device, a sample concentrating and storing device, a sample recording device and a second ultraviolet sterilizing device, wherein the sample filtering and impurity removing device comprises a precipitating device, a filtering device, a storing device and a processing device, the device is provided with ultraviolet sterilization to realize aseptic operation and really realize sterilization, ensure no pollution operation of samples in the operation process, solve the problems of pollution of the samples in the road and the like caused by remote road in the actual production operation, and has simple structure, convenient operation and strong practicability, the sample recording device records the quantity, quality and temperature of the samples, is convenient to check and record the conditions of the samples in the future, and lightens the working intensity of workers, the working environment quality of workers is improved, and potential safety hazards are reduced.
Description
Technical Field
The invention relates to a modular pretreatment system for microbial detection.
Background
The microorganism detection must be performed in the P2 laboratory, and the construction of the P2 laboratory is very strict and requires a lot of time and money, resulting in that the P2 laboratory cannot be popularized to various towns and remote areas. However, in practical supervision, problems such as remote roads and the like may cause uncertain factors such as contamination of samples in the roads. How to provide a scheme for meeting the related requirements of a P2 laboratory on cleanliness, pressure difference and sterilization degree is a technical problem to be solved by the application.
Disclosure of Invention
The present invention is directed to solving the above deficiencies of the prior art and to providing a modular pretreatment system for microbial detection.
The utility model provides a modular be used for microbial detection's preceding processing system, includes sample reducing mechanism, sample diluting device, sample mixing arrangement, sample filtration edulcoration device, sample sorter, the concentrated storage device of sample, sample recorder, second ultraviolet sterilization device, the sample filtration edulcoration device including sediment device, filter equipment, storage device, processing apparatus.
As a further improvement, a first ultraviolet sterilization device is arranged in front of the pretreatment system.
As a further improvement, the sample diluting device comprises a liquid adding device and a liquid storage device.
As a further improvement, the liquid is an enrichment liquid.
As a further improvement, the sample classification device is a plurality of independent sealed spaces.
As a further improvement, the sample recording device comprises a sample quantity recording device, a sample quality recording device and a sample temperature recording device.
Has the advantages that:
this device sets up ultraviolet sterilization, realize aseptic technique, really realize the sterilization, guarantee that the sample is pollution-free operation in operation process, solved in the actual production operation because the road is far away, lead to the sample to pollute the scheduling problem in the road, moreover, the steam generator is simple in structure, the operation of being convenient for, therefore, the clothes hanger is strong in practicability, sample recorder carries out the record to quantity, quality, the temperature of sample, be convenient for inspect and the record the condition of sample in the future, alleviate staff's working strength, improve staff's operational environment quality, and the potential safety hazard is reduced.
Drawings
FIG. 1 is a schematic diagram of the general construction of a modular pretreatment system for microbial detection;
Detailed Description
For the purpose of enhancing the understanding of the present invention, the present invention will be further described in detail with reference to the following examples and the accompanying drawings, which are only used for explaining the present invention and are not to be construed as limiting the scope of the present invention.
As shown in figure 1, a modular is used for microbial detection's preceding processing system, including sample reducing mechanism, sample diluting device, sample mixing arrangement, sample filtration edulcoration device, sample sorter, the concentrated storage device of sample, sample recorder, second ultraviolet sterilization device, sample filtration edulcoration device include sediment device, filter equipment, storage device, processing apparatus, be equipped with first ultraviolet sterilization device before the preceding processing system, sample diluting device includes liquid feeding device, liquid storage device, liquid be the enrichment fluid, sample sorter is a plurality of independent confined space, sample recorder includes sample quantity recorder, sample quality recorder, sample temperature recorder.
A pretreatment method of a modular pretreatment system for microbial detection, comprising the steps of:
and A, treating the sample by a first ultraviolet sterilization device before the sample enters a sample crushing device, so as to realize aseptic operation and really realize sterilization.
And B, after the sample is sterilized, the sample enters a sample crushing device to be crushed and ground.
And C, feeding the sample after passing through the sample crushing device into a sample diluting device, adding liquid into the sample through a liquid adding device in the sample diluting device, and adding liquid into the sample from the liquid storage device, wherein the added liquid is enrichment liquid.
And D, performing sedimentation and filtration on the sample added with the enrichment fluid, performing sedimentation and filtration on the extracted sample, and then performing classification treatment, and the method is simple in structure, convenient to operate and high in practicability.
And E, entering a sample concentration and storage device, concentrating the cultured solution containing the functional bacteria by the sample concentration and storage device, and independently sealing and storing the concentrated solution, so that the problems of sample pollution in the road and the like caused by remote road in the actual production operation are solved.
And F, recording and storing the quantity, the quality and the temperature of the sample by the sample recording device comprising a sample quantity recording device, a sample quality recording device and a sample temperature recording device, so that the quantity, the quality and the temperature of the sample can be conveniently checked and recorded in the future, the working strength of workers is reduced, the working environment quality of the workers is improved, and potential safety hazards are reduced.
And G, sterilizing the internal environment by using a second ultraviolet sterilization device after the sample is processed.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.
Claims (6)
1. The utility model provides a modular be used for microbial detection's preceding processing system, its characterized in that includes sample reducing mechanism, sample diluting device, sample mixing arrangement, sample filtration edulcoration device, sample sorter, the concentrated storage device of sample, sample recorder, second ultraviolet sterilization device, sample filtration edulcoration device including sediment device, filter equipment, storage device, processing apparatus.
2. The modular pretreatment system for microbial detection of claim 1, wherein the pretreatment system is preceded by a first ultraviolet sterilization device.
3. The modular pretreatment system for microbial detection of claim 1, wherein said sample dilution device comprises a priming device and a liquid storage device.
4. The modular pretreatment system for microbial detection of claim 3, wherein said liquid is an enrichment liquid.
5. The modular pretreatment system for microbial detection according to claim 1, wherein said sample sorting device comprises a plurality of independent sealed spaces.
6. The modular pretreatment system for microbial detection of claim 1, wherein said sample recording device comprises a sample quantity recording device, a sample quality recording device, and a sample temperature recording device.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110713915.7A CN113430107A (en) | 2021-06-25 | 2021-06-25 | Modular pretreatment system for microbial detection |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110713915.7A CN113430107A (en) | 2021-06-25 | 2021-06-25 | Modular pretreatment system for microbial detection |
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| Publication Number | Publication Date |
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| CN113430107A true CN113430107A (en) | 2021-09-24 |
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| CN202110713915.7A Pending CN113430107A (en) | 2021-06-25 | 2021-06-25 | Modular pretreatment system for microbial detection |
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB1503828A (en) * | 1976-06-22 | 1978-03-15 | Univ Strathclyde | Method of enumerating bacteria |
| GB9503428D0 (en) * | 1995-02-21 | 1995-04-12 | Mini Agriculture & Fisheries | Test for bacteria |
| CN103765193A (en) * | 2011-06-22 | 2014-04-30 | 载泽吧检测有限公司 | Micro-organism threat detection |
| US20160122796A1 (en) * | 2013-05-29 | 2016-05-05 | Satake Corporation | Method for examining microorganisms |
| CN107449652A (en) * | 2016-08-31 | 2017-12-08 | 赖晓健 | A kind of three-dimensional intelligent liquid dosage system |
| CN108410694A (en) * | 2018-02-11 | 2018-08-17 | 张家口健垣科技有限公司 | A kind of preprocessing system and method for microorganism detection |
-
2021
- 2021-06-25 CN CN202110713915.7A patent/CN113430107A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB1503828A (en) * | 1976-06-22 | 1978-03-15 | Univ Strathclyde | Method of enumerating bacteria |
| GB9503428D0 (en) * | 1995-02-21 | 1995-04-12 | Mini Agriculture & Fisheries | Test for bacteria |
| CN103765193A (en) * | 2011-06-22 | 2014-04-30 | 载泽吧检测有限公司 | Micro-organism threat detection |
| US20160122796A1 (en) * | 2013-05-29 | 2016-05-05 | Satake Corporation | Method for examining microorganisms |
| CN107449652A (en) * | 2016-08-31 | 2017-12-08 | 赖晓健 | A kind of three-dimensional intelligent liquid dosage system |
| CN108410694A (en) * | 2018-02-11 | 2018-08-17 | 张家口健垣科技有限公司 | A kind of preprocessing system and method for microorganism detection |
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