CN108410694A - A kind of preprocessing system and method for microorganism detection - Google Patents
A kind of preprocessing system and method for microorganism detection Download PDFInfo
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- CN108410694A CN108410694A CN201810142015.XA CN201810142015A CN108410694A CN 108410694 A CN108410694 A CN 108410694A CN 201810142015 A CN201810142015 A CN 201810142015A CN 108410694 A CN108410694 A CN 108410694A
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- solution
- sample
- function bacterium
- sedimentation
- microorganism detection
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M45/00—Means for pre-treatment of biological substances
- C12M45/02—Means for pre-treatment of biological substances by mechanical forces; Stirring; Trituration; Comminuting
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M45/00—Means for pre-treatment of biological substances
Abstract
The invention discloses a kind of preprocessing systems and method for microorganism detection, and wherein system includes being provided with the confined space of the ventilation opening and sample comminution apparatus being arranged in confined space, sedimentation and filter device and sample concentration device；Sample comminution apparatus is crushed for the sample to acquisition；Sedimentation and filter device are extracted after sedimentation and filtering, and include the solution of function bacterium, are cultivated the solution comprising function bacterium for the solution after crushing to be settled and filtered；Sample concentration device carries out lid lid to the solution after concentration and independently seals up for safekeeping to solve in practical operation due to far away, lead to problems such as sample be polluted in road, with very high practicability for being concentrated to the solution comprising function bacterium after culture.
Description
Technical field
The present invention relates to a kind of microbiological treatment field, more particularly to a kind of preprocessing system for microorganism detection and
Method.
Background technology
Microorganism detection must carry out in P2 Lab, and what the construction of P2 Lab was strict needs the plenty of time
And money, it cannot spread to each small towns and remote districts so as to cause P2 Lab.However it may be due in onsite supervision
The problems such as far away, may cause sample the uncertain factors such as to pollute in road.How a kind of cleanliness factor to be provided, pressure difference with
And it is the application technical problems to be solved that sterility, which reaches the scheme of the related request of P2 Lab,.
Invention content
In view of the above technical problems, the present invention provides a kind of cleanliness factor, and pressure difference and sterility reach P2 Lab
The preprocessing system and method for microorganism detection of related request.
In order to solve the above technical problems, the technical solution used in the present invention is：It provides a kind of for microorganism detection
Preprocessing system, including it is provided with the confined space of ventilation opening and the sample comminution apparatus being arranged in confined space, sedimentation
And filter device, sample concentration device；
The sample comminution apparatus, for after enrichment liquid is added, being crushed to the sample of acquisition, to molten after crushing
Liquid culture；
The sedimentation and filter device, for the solution after culture to be settled and filtered, extraction is by sedimentation and mistake
After filter, and include the solution of function bacterium, the solution comprising function bacterium is cultivated；
The sample concentration device, for being concentrated to the solution comprising function bacterium after culture, after concentration
Solution carry out even group-division, and the sample solution after grouping is stored in separate space, and seal.
More preferably, in the above-mentioned technical solutions, the preprocessing system further includes enrichment liquid storage device；
The enrichment liquid storage device is added to the sedimentation and mistake for storing enrichment liquid, and by the enrichment liquid of storage
It filters in device.
More preferably, in the above-mentioned technical solutions, the preprocessing system further includes being arranged in confined space vent position
Filter；
The filter is filtered for pair air to circulate with the confined space.
More preferably, in the above-mentioned technical solutions, the preprocessing system further includes ultraviolet sterilizer；
The ultraviolet sterilizer is arranged at the top of the confined space, for the sample comminution apparatus, institute
It states sedimentation and filter device, the sample concentration device, the enrichment liquid storage device carries out disinfection sterilization.
More preferably, in the above-mentioned technical solutions, the ultraviolet sterilizer is arranged at the top of the confined space, uses
In to the sample comminution apparatus, it is described sedimentation and filter device, the sample concentration device, the enrichment liquid storage device into
Row disinfection.
The present invention also provides a kind of pre-treating methods for microorganism detection, include the following steps：
The sample of acquisition is crushed, extraction crushes solution；
It cultivated, settled and is filtered to crushing solution, extraction is after sedimentation and filtering and includes the molten of function bacterium
Liquid cultivates the solution comprising function bacterium；；
The solution comprising function bacterium after culture is concentrated, even group-division is carried out to the solution after concentration, and
Sample solution after grouping, which is stored in, which can carry out the separate space inner cover lid of air circulation, seals up for safekeeping.
More preferably, in the above-mentioned technical solutions, in the solution for extracting after sedimentation and filtering and include function bacterium
Later, before cultivating the solution comprising function bacterium, further include：
Enrichment liquid is added in the solution comprising function bacterium.
More preferably, in the above-mentioned technical solutions, the pre-treating method further includes：
Disinfection by ultraviolet light is carried out to the internal environment control before and after sample treatment.
Using above technical scheme, the technique effect reached is the present invention：It is provided by the invention for microorganism detection
Preprocessing system and method, cleanliness factor, pressure difference and sterility have reached the related request of P2 Lab, solve practical behaviour
Due to far away in work, leads to problems such as sample be polluted in road, expand the preprocessing system for microorganism detection
And the scope of application of method, there is very high practicability.
Description of the drawings
The invention will be further described below in conjunction with the accompanying drawings：
Fig. 1 is the schematic frame diagram of the preprocessing system provided by the invention for microorganism detection；
Fig. 2 is the schematic flow chart of the pre-treating method provided by the invention for microorganism detection.
Specific implementation mode
As shown in Figure 1, the preprocessing system provided by the invention for microorganism detection, including it is provided with the close of ventilation opening
Sample comminution apparatus, sedimentation and the filter device and sample concentration device for closing space and being arranged in confined space；
The sample comminution apparatus, for after enrichment liquid is added, being crushed to the sample of acquisition, to molten after crushing
Liquid culture；
The sedimentation and filter device, for the solution after culture to be settled and filtered, extraction is by sedimentation and mistake
After filter, and include the solution of function bacterium, the solution comprising function bacterium is cultivated；
The sample concentration device, for being concentrated to the solution comprising function bacterium after culture, after concentration
Solution carry out even group-division, and the sample solution after grouping is stored in separate space, and seal.
As a kind of embodiment, preprocessing system further includes enrichment liquid storage device；
Enrichment liquid storage device is added to for storing enrichment liquid, and by the enrichment liquid of storage in sedimentation and filter device.
As a kind of embodiment, preprocessing system further includes the filter being arranged in confined space vent position；
Filter with the air that confined space circulates for pair being filtered.
As a kind of embodiment, preprocessing system further includes ultraviolet sterilizer；
Ultraviolet sterilizer is arranged at the top of confined space, for sample comminution apparatus, sedimentation and filter device,
Sample concentration device, enrichment liquid storage device carry out disinfection sterilization.
As shown in Fig. 2, the present invention also provides a kind of pre-treating method for microorganism detection, include the following steps：
The sample of acquisition is crushed, extraction crushes solution；
It carries out, settle and filters to crushing solution, extract after sedimentation and filtering；
The solution comprising function bacterium after culture is concentrated, even group-division is carried out to the solution after concentration, and
Sample solution after grouping is stored in separate space, and is sealed.
As a kind of embodiment, extraction after sedimentation and filtering and include function bacterium solution after, to packet
Before solution containing function bacterium is cultivated, further include：
Enrichment liquid is added in the solution comprising function bacterium.
As a kind of embodiment, pre-treating method further includes：
Disinfection by ultraviolet light is carried out to the internal environment control before and after sample treatment.
Using above technical scheme, the technique effect reached is the present invention：It is provided by the invention for microorganism detection
Preprocessing system and method, cleanliness factor, pressure difference and sterility have reached the related request of P2 Lab, solve practical behaviour
Due to far away in work, leads to problems such as sample be polluted in road, expand the preprocessing system for microorganism detection
And the scope of application of method, there is very high practicability.
It can be that professional and technical personnel in the field realize or use that the above embodiment, which is intended to illustrate the present invention, to above-mentioned
Embodiment, which is modified, will be apparent to those skilled in the art, therefore the present invention includes but not limited to
The above embodiment, it is any to meet the claims or specification description, meet with principles disclosed herein and novelty,
The method of inventive features, technique, product, each fall within protection scope of the present invention.
Claims (7)
1. a kind of preprocessing system for microorganism detection, it is characterised in that：Including be provided with the confined space of ventilation opening with
And sample comminution apparatus, sedimentation and the filter device and sample concentration device being arranged in confined space；
The sample comminution apparatus trains the solution after crushing for after enrichment liquid is added, being crushed to the sample of acquisition
It supports；
The sedimentation and filter device extract after sedimentation and filtering for the solution after culture to be settled and filtered,
And include the solution of function bacterium, the solution comprising function bacterium is cultivated；
The sample concentration device, for being concentrated to the solution comprising function bacterium after culture, to molten after concentration
Liquid carries out even group-division, and the sample solution after grouping is stored in separate space, and seals.
2. being directed to the preprocessing system of microorganism detection as described in claim 1, it is characterised in that：The preprocessing system is also
Including enrichment liquid storage device；
The enrichment liquid storage device is added to the sedimentation and filtering dress for storing enrichment liquid, and by the enrichment liquid of storage
In setting.
3. being directed to the preprocessing system of microorganism detection as claimed in claim 2, it is characterised in that：The preprocessing system is also
Including the filter in confined space vent position is arranged；
The filter is filtered for pair air to circulate with the confined space.
4. being directed to the preprocessing system of microorganism detection as claimed in claim 3, it is characterised in that：The preprocessing system is also
Including ultraviolet sterilizer；
The ultraviolet sterilizer is arranged at the top of the confined space, for the sample comminution apparatus, described heavy
Drop and filter device, the sample concentration device, the enrichment liquid storage device carry out disinfection sterilization.
5. a kind of pre-treating method for microorganism detection, which is characterized in that include the following steps：
Acquisition sample after addition enrichment liquid is crushed, to the hydroponics after crushing；
Solution after culture is settled and filtered, is extracted after sedimentation and filtering, and include the solution of function bacterium, to packet
Solution containing function bacterium is cultivated；
The solution comprising function bacterium after culture is concentrated, even group-division is carried out to the solution after concentration, and will divide
Sample solution after group is stored in separate space, and is sealed.
6. being directed to the pre-treating method of microorganism detection as claimed in claim 5, it is characterised in that：In the extraction by heavy
Drop and filtering after and include function bacterium solution after, before cultivating the solution comprising function bacterium, further include：
Enrichment liquid is added in the solution comprising function bacterium.
7. being directed to the pre-treating method of microorganism detection as claimed in claim 6, it is characterised in that：The pre-treating method is also
Including：
Disinfection by ultraviolet light is carried out to the internal environment control before and after sample treatment.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201810142015.XA CN108410694A (en) | 2018-02-11 | 2018-02-11 | A kind of preprocessing system and method for microorganism detection |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201810142015.XA CN108410694A (en) | 2018-02-11 | 2018-02-11 | A kind of preprocessing system and method for microorganism detection |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109082456A (en) * | 2018-08-24 | 2018-12-25 | 张家口健垣科技有限公司 | A kind of automated food microorganism detection pre-treating method and device |
| CN113430107A (en) * | 2021-06-25 | 2021-09-24 | 常州市金坛第一人民医院 | Modular pretreatment system for microbial detection |
| CN113893908A (en) * | 2021-09-27 | 2022-01-07 | 南京嘉通微纳科技有限公司 | Microbial detection pretreatment device and microbial detector comprising same |
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| CN206089654U (en) * | 2016-09-28 | 2017-04-12 | 西安工业大学 | Bacterium detecting device |
| CN106831149A (en) * | 2017-02-25 | 2017-06-13 | 湖南隆农农业科技开发有限公司 | A kind of micro-organism fertilizer for improving soil se use efficiency and preparation method thereof |
| CN107099598A (en) * | 2017-05-24 | 2017-08-29 | 济南市疾病预防控制中心 | A kind of method of the micro-fluidic integrated detection of bacterium |
| US20170318762A1 (en) * | 2016-05-09 | 2017-11-09 | Chi-Tse Kuo | Method and system of cultivating aquatic product and plant |
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2018
- 2018-02-11 CN CN201810142015.XA patent/CN108410694A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20170318762A1 (en) * | 2016-05-09 | 2017-11-09 | Chi-Tse Kuo | Method and system of cultivating aquatic product and plant |
| CN206089654U (en) * | 2016-09-28 | 2017-04-12 | 西安工业大学 | Bacterium detecting device |
| CN106831149A (en) * | 2017-02-25 | 2017-06-13 | 湖南隆农农业科技开发有限公司 | A kind of micro-organism fertilizer for improving soil se use efficiency and preparation method thereof |
| CN107099598A (en) * | 2017-05-24 | 2017-08-29 | 济南市疾病预防控制中心 | A kind of method of the micro-fluidic integrated detection of bacterium |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109082456A (en) * | 2018-08-24 | 2018-12-25 | 张家口健垣科技有限公司 | A kind of automated food microorganism detection pre-treating method and device |
| CN109082456B (en) * | 2018-08-24 | 2021-07-23 | 张家口健垣科技有限公司 | Automatic food microorganism detection pretreatment method and device |
| CN113430107A (en) * | 2021-06-25 | 2021-09-24 | 常州市金坛第一人民医院 | Modular pretreatment system for microbial detection |
| CN113893908A (en) * | 2021-09-27 | 2022-01-07 | 南京嘉通微纳科技有限公司 | Microbial detection pretreatment device and microbial detector comprising same |
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Address after: 075000 No. 8, Xingye Road, industrial cluster district, Zhangjiakou City, Hebei Province Applicant after: Jianyuan Technology (Zhangjiakou) Co., Ltd Address before: 075000 No. 8, Xingye Road, industrial cluster district, Zhangjiakou City, Hebei Province Applicant before: Zhangjiakou Jianyuan Technology Co., Ltd |
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Application publication date: 20180817 |
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