DE2634728A1 - Zellkulturmedium - Google Patents
ZellkulturmediumInfo
- Publication number
- DE2634728A1 DE2634728A1 DE19762634728 DE2634728A DE2634728A1 DE 2634728 A1 DE2634728 A1 DE 2634728A1 DE 19762634728 DE19762634728 DE 19762634728 DE 2634728 A DE2634728 A DE 2634728A DE 2634728 A1 DE2634728 A1 DE 2634728A1
- Authority
- DE
- Germany
- Prior art keywords
- serum
- cells
- culture medium
- culture
- pig
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
- 239000006143 cell culture medium Substances 0.000 title description 3
- 210000002966 serum Anatomy 0.000 claims description 53
- 239000002609 medium Substances 0.000 claims description 16
- 239000001963 growth medium Substances 0.000 claims description 15
- 239000004094 surface-active agent Substances 0.000 claims description 10
- 238000000034 method Methods 0.000 claims description 8
- 241001465754 Metazoa Species 0.000 claims description 5
- 229920000609 methyl cellulose Polymers 0.000 claims description 5
- 239000001923 methylcellulose Substances 0.000 claims description 5
- 235000010981 methylcellulose Nutrition 0.000 claims description 5
- 235000015097 nutrients Nutrition 0.000 claims description 5
- 230000006920 protein precipitation Effects 0.000 claims description 4
- 230000002401 inhibitory effect Effects 0.000 claims description 3
- 229920002503 polyoxyethylene-polyoxypropylene Polymers 0.000 claims description 3
- 210000004027 cell Anatomy 0.000 description 32
- 230000012010 growth Effects 0.000 description 12
- 244000309466 calf Species 0.000 description 11
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 8
- 239000012894 fetal calf serum Substances 0.000 description 8
- 210000004102 animal cell Anatomy 0.000 description 5
- 230000001605 fetal effect Effects 0.000 description 5
- 102000014150 Interferons Human genes 0.000 description 4
- 108010050904 Interferons Proteins 0.000 description 4
- 235000019687 Lamb Nutrition 0.000 description 4
- 230000010261 cell growth Effects 0.000 description 4
- 229940079322 interferon Drugs 0.000 description 4
- 210000004698 lymphocyte Anatomy 0.000 description 4
- 241000287828 Gallus gallus Species 0.000 description 3
- 239000002054 inoculum Substances 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 229920000642 polymer Polymers 0.000 description 3
- 108010017384 Blood Proteins Proteins 0.000 description 2
- 102000004506 Blood Proteins Human genes 0.000 description 2
- 239000000427 antigen Substances 0.000 description 2
- 102000036639 antigens Human genes 0.000 description 2
- 108091007433 antigens Proteins 0.000 description 2
- 210000004748 cultured cell Anatomy 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- -1 polyoxypropylene Polymers 0.000 description 2
- 238000001556 precipitation Methods 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 241000083551 Ena Species 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- IAYPIBMASNFSPL-UHFFFAOYSA-N Ethylene oxide Chemical compound C1CO1 IAYPIBMASNFSPL-UHFFFAOYSA-N 0.000 description 1
- 102000008072 Lymphokines Human genes 0.000 description 1
- 108010074338 Lymphokines Proteins 0.000 description 1
- 239000006146 Roswell Park Memorial Institute medium Substances 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 239000013065 commercial product Substances 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- HQPMKSGTIOYHJT-UHFFFAOYSA-N ethane-1,2-diol;propane-1,2-diol Chemical compound OCCO.CC(O)CO HQPMKSGTIOYHJT-UHFFFAOYSA-N 0.000 description 1
- 210000005260 human cell Anatomy 0.000 description 1
- 230000002209 hydrophobic effect Effects 0.000 description 1
- 238000009630 liquid culture Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 239000000693 micelle Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 229920001983 poloxamer Polymers 0.000 description 1
- 229920001993 poloxamer 188 Polymers 0.000 description 1
- 229920001451 polypropylene glycol Polymers 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 238000012807 shake-flask culturing Methods 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 238000004114 suspension culture Methods 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0634—Cells from the blood or the immune system
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/50—Soluble polymers, e.g. polyethyleneglycol [PEG]
Landscapes
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Hematology (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Cell Biology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB32237/75A GB1540579A (en) | 1975-08-01 | 1975-08-01 | Cell culture medium |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DE2634728A1 true DE2634728A1 (de) | 1977-02-17 |
Family
ID=10335466
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE19762634728 Withdrawn DE2634728A1 (de) | 1975-08-01 | 1976-08-02 | Zellkulturmedium |
Country Status (5)
| Country | Link |
|---|---|
| JP (1) | JPS5218880A (enrdf_load_stackoverflow) |
| BE (1) | BE844763A (enrdf_load_stackoverflow) |
| DE (1) | DE2634728A1 (enrdf_load_stackoverflow) |
| FR (1) | FR2319710A1 (enrdf_load_stackoverflow) |
| GB (1) | GB1540579A (enrdf_load_stackoverflow) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FI820200L (fi) * | 1981-01-28 | 1982-07-29 | Biotechnolog Forschung Gmbh | Foerfarande foer framstaellning av en antikropp producerande hybridceller och av antikroppar hybridceller och antikroppar |
| FR2572093A1 (fr) * | 1984-10-22 | 1986-04-25 | Anda Biolog | Nouveau milieu de culture cellulaire contenant des cellules de rate humaine, procede de culture utilisant ce milieu de culture, lignees de cellules lymphoblastoides obtenues, application a la production d'interferon, procede de preparation d'interferon et interferon ainsi produit |
| JP3339566B2 (ja) | 1998-10-21 | 2002-10-28 | 日本電気株式会社 | サーモメトリック−バイナリコード変換方法および回路、それに使用されるエンコーダ素子回路 |
| WO2020022511A1 (ja) * | 2018-07-27 | 2020-01-30 | 味の素株式会社 | 動物細胞の浮遊培養用の添加物、浮遊培養用培地および浮遊培養方法 |
-
1975
- 1975-08-01 GB GB32237/75A patent/GB1540579A/en not_active Expired
-
1976
- 1976-07-30 BE BE169455A patent/BE844763A/xx unknown
- 1976-07-30 JP JP51091217A patent/JPS5218880A/ja active Pending
- 1976-07-30 FR FR7623440A patent/FR2319710A1/fr active Granted
- 1976-08-02 DE DE19762634728 patent/DE2634728A1/de not_active Withdrawn
Also Published As
| Publication number | Publication date |
|---|---|
| BE844763A (fr) | 1977-01-31 |
| JPS5218880A (en) | 1977-02-12 |
| GB1540579A (en) | 1979-02-14 |
| FR2319710A1 (fr) | 1977-02-25 |
| FR2319710B1 (enrdf_load_stackoverflow) | 1979-06-22 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 8139 | Disposal/non-payment of the annual fee |