DE2514225C2 - Verfahren zur Herstellung immobilisierter Enzyme durch chemische Bindung an anorganische Träger - Google Patents
Verfahren zur Herstellung immobilisierter Enzyme durch chemische Bindung an anorganische TrägerInfo
- Publication number
- DE2514225C2 DE2514225C2 DE2514225A DE2514225A DE2514225C2 DE 2514225 C2 DE2514225 C2 DE 2514225C2 DE 2514225 A DE2514225 A DE 2514225A DE 2514225 A DE2514225 A DE 2514225A DE 2514225 C2 DE2514225 C2 DE 2514225C2
- Authority
- DE
- Germany
- Prior art keywords
- enzyme
- solution
- porous
- water
- added
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 238000000034 method Methods 0.000 title claims description 7
- 108010093096 Immobilized Enzymes Proteins 0.000 title claims description 6
- 238000004519 manufacturing process Methods 0.000 title claims description 6
- 239000000969 carrier Substances 0.000 title description 9
- 239000000126 substance Substances 0.000 title description 3
- 102000004190 Enzymes Human genes 0.000 claims description 41
- 108090000790 Enzymes Proteins 0.000 claims description 39
- 239000011148 porous material Substances 0.000 claims description 25
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 19
- 239000002245 particle Substances 0.000 claims description 17
- 239000000758 substrate Substances 0.000 claims description 10
- 239000000377 silicon dioxide Substances 0.000 claims description 9
- 125000000751 azo group Chemical group [*]N=N[*] 0.000 claims description 7
- 239000000203 mixture Substances 0.000 claims description 7
- 239000005373 porous glass Substances 0.000 claims description 6
- TWNQGVIAIRXVLR-UHFFFAOYSA-N oxo(oxoalumanyloxy)alumane Chemical compound O=[Al]O[Al]=O TWNQGVIAIRXVLR-UHFFFAOYSA-N 0.000 claims description 2
- RDOXTESZEPMUJZ-UHFFFAOYSA-N anisole Chemical compound COC1=CC=CC=C1 RDOXTESZEPMUJZ-UHFFFAOYSA-N 0.000 claims 1
- 239000000243 solution Substances 0.000 description 39
- 229940088598 enzyme Drugs 0.000 description 38
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 28
- 230000000694 effects Effects 0.000 description 9
- 239000004365 Protease Substances 0.000 description 8
- 239000011521 glass Substances 0.000 description 8
- 230000008878 coupling Effects 0.000 description 7
- 238000010168 coupling process Methods 0.000 description 7
- 238000005859 coupling reaction Methods 0.000 description 7
- 238000002360 preparation method Methods 0.000 description 7
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 6
- 239000012153 distilled water Substances 0.000 description 6
- 108091005804 Peptidases Proteins 0.000 description 5
- 229940079919 digestives enzyme preparation Drugs 0.000 description 5
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 4
- VEXZGXHMUGYJMC-UHFFFAOYSA-M Chloride anion Chemical compound [Cl-] VEXZGXHMUGYJMC-UHFFFAOYSA-M 0.000 description 4
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 4
- 229910044991 metal oxide Inorganic materials 0.000 description 4
- 150000004706 metal oxides Chemical class 0.000 description 4
- 235000019419 proteases Nutrition 0.000 description 4
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 3
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 3
- 229910019142 PO4 Inorganic materials 0.000 description 3
- 108090000526 Papain Proteins 0.000 description 3
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 3
- 239000004202 carbamide Substances 0.000 description 3
- 239000005018 casein Substances 0.000 description 3
- BECPQYXYKAMYBN-UHFFFAOYSA-N casein, tech. Chemical compound NCCCCC(C(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(CC(C)C)N=C(O)C(CCC(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(C(C)O)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(COP(O)(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(N)CC1=CC=CC=C1 BECPQYXYKAMYBN-UHFFFAOYSA-N 0.000 description 3
- 235000021240 caseins Nutrition 0.000 description 3
- 229960001484 edetic acid Drugs 0.000 description 3
- 239000008103 glucose Substances 0.000 description 3
- 229940055729 papain Drugs 0.000 description 3
- 235000019834 papain Nutrition 0.000 description 3
- 239000010452 phosphate Substances 0.000 description 3
- 239000008363 phosphate buffer Substances 0.000 description 3
- 150000003839 salts Chemical class 0.000 description 3
- 239000011780 sodium chloride Substances 0.000 description 3
- 238000001179 sorption measurement Methods 0.000 description 3
- 241000193830 Bacillus <bacterium> Species 0.000 description 2
- CPLXHLVBOLITMK-UHFFFAOYSA-N Magnesium oxide Chemical compound [Mg]=O CPLXHLVBOLITMK-UHFFFAOYSA-N 0.000 description 2
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 description 2
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 description 2
- 102000005158 Subtilisins Human genes 0.000 description 2
- 108010056079 Subtilisins Proteins 0.000 description 2
- 108010046334 Urease Proteins 0.000 description 2
- 108700040099 Xylose isomerases Proteins 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 238000005054 agglomeration Methods 0.000 description 2
- 230000002776 aggregation Effects 0.000 description 2
- 239000007864 aqueous solution Substances 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 239000007822 coupling agent Substances 0.000 description 2
- XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 description 2
- 235000018417 cysteine Nutrition 0.000 description 2
- QTBSBXVTEAMEQO-UHFFFAOYSA-M Acetate Chemical compound CC([O-])=O QTBSBXVTEAMEQO-UHFFFAOYSA-M 0.000 description 1
- 229910018072 Al 2 O 3 Inorganic materials 0.000 description 1
- -1 B. SlO 2 -AbO 3 Chemical class 0.000 description 1
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 1
- PIICEJLVQHRZGT-UHFFFAOYSA-N Ethylenediamine Chemical compound NCCN PIICEJLVQHRZGT-UHFFFAOYSA-N 0.000 description 1
- 102000020897 Formins Human genes 0.000 description 1
- 108091022623 Formins Proteins 0.000 description 1
- 229930091371 Fructose Natural products 0.000 description 1
- 239000005715 Fructose Substances 0.000 description 1
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 description 1
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 1
- 206010061217 Infestation Diseases 0.000 description 1
- XUJNEKJLAYXESH-REOHCLBHSA-N L-Cysteine Chemical compound SC[C@H](N)C(O)=O XUJNEKJLAYXESH-REOHCLBHSA-N 0.000 description 1
- 235000017858 Laurus nobilis Nutrition 0.000 description 1
- 102000035195 Peptidases Human genes 0.000 description 1
- 229910004298 SiO 2 Inorganic materials 0.000 description 1
- 235000005212 Terminalia tomentosa Nutrition 0.000 description 1
- 244000125380 Terminalia tomentosa Species 0.000 description 1
- 150000004982 aromatic amines Chemical class 0.000 description 1
- 241001233037 catfish Species 0.000 description 1
- 150000003841 chloride salts Chemical class 0.000 description 1
- 239000013065 commercial product Substances 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000007334 copolymerization reaction Methods 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 229960001760 dimethyl sulfoxide Drugs 0.000 description 1
- 238000005538 encapsulation Methods 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 125000000524 functional group Chemical group 0.000 description 1
- 238000000227 grinding Methods 0.000 description 1
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 1
- 238000005342 ion exchange Methods 0.000 description 1
- 239000000395 magnesium oxide Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 230000035515 penetration Effects 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 235000018102 proteins Nutrition 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 239000011541 reaction mixture Substances 0.000 description 1
- 150000004756 silanes Chemical class 0.000 description 1
- 238000005245 sintering Methods 0.000 description 1
- 239000002002 slurry Substances 0.000 description 1
- 229910000030 sodium bicarbonate Inorganic materials 0.000 description 1
- 235000017557 sodium bicarbonate Nutrition 0.000 description 1
- 229910000029 sodium carbonate Inorganic materials 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 238000001694 spray drying Methods 0.000 description 1
- 230000008961 swelling Effects 0.000 description 1
- 125000001493 tyrosinyl group Chemical group [H]OC1=C([H])C([H])=C(C([H])=C1[H])C([H])([H])C([H])(N([H])[H])C(*)=O 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/14—Enzymes or microbial cells immobilised on or in an inorganic carrier
Landscapes
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Inorganic Chemistry (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US05/473,717 US3930951A (en) | 1974-05-28 | 1974-05-28 | Bonding enzymes to porous inorganic carriers |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DE2514225A1 DE2514225A1 (de) | 1975-12-11 |
| DE2514225C2 true DE2514225C2 (de) | 1985-08-08 |
Family
ID=23880695
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE2514225A Expired DE2514225C2 (de) | 1974-05-28 | 1975-04-01 | Verfahren zur Herstellung immobilisierter Enzyme durch chemische Bindung an anorganische Träger |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US3930951A ( ) |
| JP (1) | JPS5819274B2 ( ) |
| CA (1) | CA1037399A ( ) |
| DE (1) | DE2514225C2 ( ) |
| FR (1) | FR2273008B1 ( ) |
| GB (1) | GB1457993A ( ) |
| IT (1) | IT1038178B ( ) |
Families Citing this family (20)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3983000A (en) * | 1976-04-01 | 1976-09-28 | Corning Glass Works | Bonding proteins to inorganic supports |
| JPS52120190A (en) * | 1976-04-02 | 1977-10-08 | Cpc International Inc | Fixing method of glucose isomerase and cotinuous isomerization of glucose |
| US4107287A (en) * | 1976-04-12 | 1978-08-15 | University Of Pennsylvania | GC Antigen-specific immunochemical indicator reagent and method for preparing same |
| US4072566A (en) * | 1976-09-27 | 1978-02-07 | Corning Glass Works | Immobilized biologically active proteins |
| DE2726188C2 (de) * | 1977-06-10 | 1979-05-10 | Kali-Chemie Ag, 3000 Hannover | Verfahren zur Herstellung eines wasserunlöslichen Enzympräparats |
| GB2004300B (en) * | 1977-09-14 | 1982-08-04 | Corning Glass Works | High surface low volume biomass composites |
| US4206286A (en) * | 1977-11-14 | 1980-06-03 | Technicon Instruments Corporation | Immobilization of proteins on inorganic supports |
| US4204040A (en) * | 1977-11-18 | 1980-05-20 | Technicon Instruments Corporation | Copolymerization of proteins on an inorganic support |
| JPS5849537B2 (ja) * | 1980-03-18 | 1983-11-05 | 三菱化学株式会社 | 尿素の製造方法 |
| US4748121A (en) * | 1984-11-30 | 1988-05-31 | Ppg Industries, Inc. | Porous glass fibers with immobilized biochemically active material |
| US4861714A (en) * | 1985-04-04 | 1989-08-29 | Verax Corporation | Weighted collagen microsponge for immobilizing bioactive material |
| US5100783A (en) * | 1985-05-10 | 1992-03-31 | Verax Corporation | Weighted microsponge for immobilizing bioactive material |
| IL93134A (en) * | 1990-01-23 | 1997-11-20 | Yissum Res Dev Co | Doped sol-gel glasses for obtaining chemical interactions |
| US5371018A (en) * | 1990-01-23 | 1994-12-06 | Yissum, Research Development Company Of The Hebrew University Of Jerusalem | Qualitative and quantitative processes for reactive chemicals in liquids using doped sol-gel glasses |
| JPH0536930U (ja) * | 1991-10-18 | 1993-05-18 | アルプス電気株式会社 | 平衡型発振器の共振回路 |
| JP2735458B2 (ja) * | 1993-06-18 | 1998-04-02 | 日本碍子株式会社 | 生体触媒用担体 |
| US7008599B1 (en) * | 1999-05-10 | 2006-03-07 | Smithkline Beecham Corporation | High throughput crystal form screening workstation and method of use |
| US20110218365A1 (en) * | 2008-09-05 | 2011-09-08 | Cobalt Technologies, Inc. | Engineered light-emitting reporter genes and methods of use |
| CN102482690A (zh) | 2009-06-26 | 2012-05-30 | 钴技术有限公司 | 生物产物生产的集成系统和方法 |
| CN103387975A (zh) | 2012-05-11 | 2013-11-13 | 上海天伟生物制药有限公司 | 一种固定化环脂肽酰基转移酶及其制备方法和用途 |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US2968635A (en) * | 1958-09-19 | 1961-01-17 | Universal Oil Prod Co | Activated alumina and the use thereof |
| US3666627A (en) * | 1968-10-14 | 1972-05-30 | Corning Glass Works | Method of stabilizing enzymes |
| US3669841A (en) * | 1970-02-11 | 1972-06-13 | Monsanto Co | Attachment of enzymes to siliceous materials |
| US3705084A (en) * | 1970-03-18 | 1972-12-05 | Monsanto Co | Macroporous enzyme reactor |
| US3783101A (en) * | 1972-02-17 | 1974-01-01 | Corning Glass Works | Enzymes bound to carriers having a metal oxide surface layer |
| US3804719A (en) * | 1972-08-07 | 1974-04-16 | Corning Glass Works | Adsorbing and crosslinking enzymes within the pores of porous glass |
-
1974
- 1974-05-28 US US05/473,717 patent/US3930951A/en not_active Expired - Lifetime
-
1975
- 1975-02-07 CA CA219,586A patent/CA1037399A/en not_active Expired
- 1975-04-01 DE DE2514225A patent/DE2514225C2/de not_active Expired
- 1975-05-16 IT IT23395/75A patent/IT1038178B/it active
- 1975-05-21 GB GB2177475A patent/GB1457993A/en not_active Expired
- 1975-05-26 JP JP50062845A patent/JPS5819274B2/ja not_active Expired
- 1975-05-27 FR FR7516419A patent/FR2273008B1/fr not_active Expired
Also Published As
| Publication number | Publication date |
|---|---|
| US3930951A (en) | 1976-01-06 |
| IT1038178B (it) | 1979-11-20 |
| FR2273008B1 ( ) | 1979-07-13 |
| JPS5819274B2 (ja) | 1983-04-16 |
| JPS511691A (en) | 1976-01-08 |
| DE2514225A1 (de) | 1975-12-11 |
| FR2273008A1 ( ) | 1975-12-26 |
| CA1037399A (en) | 1978-08-29 |
| GB1457993A (en) | 1976-12-08 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 8110 | Request for examination paragraph 44 | ||
| 8128 | New person/name/address of the agent |
Representative=s name: HERZFELD, A., RECHTSANW., 6370 OBERURSEL |
|
| D2 | Grant after examination | ||
| 8364 | No opposition during term of opposition | ||
| 8339 | Ceased/non-payment of the annual fee |