CA1037399A - Method of bonding enzymes to porous inorganic carriers - Google Patents
Method of bonding enzymes to porous inorganic carriersInfo
- Publication number
- CA1037399A CA1037399A CA219,586A CA219586A CA1037399A CA 1037399 A CA1037399 A CA 1037399A CA 219586 A CA219586 A CA 219586A CA 1037399 A CA1037399 A CA 1037399A
- Authority
- CA
- Canada
- Prior art keywords
- enzyme
- porous
- carrier
- solution
- enzymes
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 102000004190 Enzymes Human genes 0.000 title claims abstract description 76
- 108090000790 Enzymes Proteins 0.000 title claims abstract description 76
- 238000000034 method Methods 0.000 title claims abstract description 26
- 239000000969 carrier Substances 0.000 title claims abstract description 21
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims abstract description 41
- 239000011148 porous material Substances 0.000 claims abstract description 37
- 239000002245 particle Substances 0.000 claims abstract description 35
- 239000000377 silicon dioxide Substances 0.000 claims abstract description 18
- 239000000203 mixture Substances 0.000 claims abstract description 13
- PNEYBMLMFCGWSK-UHFFFAOYSA-N aluminium oxide Inorganic materials [O-2].[O-2].[O-2].[Al+3].[Al+3] PNEYBMLMFCGWSK-UHFFFAOYSA-N 0.000 claims abstract description 7
- XUEJWOZUNYWFCY-UHFFFAOYSA-M 2-methoxybenzenediazonium;chloride Chemical compound [Cl-].COC1=CC=CC=C1[N+]#N XUEJWOZUNYWFCY-UHFFFAOYSA-M 0.000 claims abstract description 4
- 229940088598 enzyme Drugs 0.000 claims description 71
- 239000002131 composite material Substances 0.000 claims description 31
- 239000004365 Protease Substances 0.000 claims description 22
- 108090000526 Papain Proteins 0.000 claims description 17
- 229940055729 papain Drugs 0.000 claims description 17
- 235000019834 papain Nutrition 0.000 claims description 17
- 108010093096 Immobilized Enzymes Proteins 0.000 claims description 15
- 239000005373 porous glass Substances 0.000 claims description 13
- 238000006243 chemical reaction Methods 0.000 claims description 10
- 239000000758 substrate Substances 0.000 claims description 10
- 108010046334 Urease Proteins 0.000 claims description 8
- 108700040099 Xylose isomerases Proteins 0.000 claims description 7
- 108091005804 Peptidases Proteins 0.000 claims description 6
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims description 6
- 235000019419 proteases Nutrition 0.000 claims description 5
- 244000063299 Bacillus subtilis Species 0.000 claims description 3
- 235000014469 Bacillus subtilis Nutrition 0.000 claims description 3
- 125000000751 azo group Chemical group [*]N=N[*] 0.000 claims description 3
- 239000000463 material Substances 0.000 abstract description 7
- 239000011521 glass Substances 0.000 abstract description 5
- 125000000664 diazo group Chemical group [N-]=[N+]=[*] 0.000 abstract description 3
- 239000000243 solution Substances 0.000 description 54
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 35
- 238000003556 assay Methods 0.000 description 15
- 230000000694 effects Effects 0.000 description 11
- 239000012153 distilled water Substances 0.000 description 9
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 description 8
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 8
- 238000010168 coupling process Methods 0.000 description 7
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 6
- 230000008878 coupling Effects 0.000 description 6
- 238000005859 coupling reaction Methods 0.000 description 6
- 229910052681 coesite Inorganic materials 0.000 description 5
- 229910052906 cristobalite Inorganic materials 0.000 description 5
- XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 description 5
- 235000018417 cysteine Nutrition 0.000 description 5
- 239000008363 phosphate buffer Substances 0.000 description 5
- 235000012239 silicon dioxide Nutrition 0.000 description 5
- 229910052682 stishovite Inorganic materials 0.000 description 5
- 229910052905 tridymite Inorganic materials 0.000 description 5
- 239000007836 KH2PO4 Substances 0.000 description 4
- 239000005018 casein Substances 0.000 description 4
- BECPQYXYKAMYBN-UHFFFAOYSA-N casein, tech. Chemical compound NCCCCC(C(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(CC(C)C)N=C(O)C(CCC(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(C(C)O)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(COP(O)(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(N)CC1=CC=CC=C1 BECPQYXYKAMYBN-UHFFFAOYSA-N 0.000 description 4
- 235000021240 caseins Nutrition 0.000 description 4
- 230000002255 enzymatic effect Effects 0.000 description 4
- 229910044991 metal oxide Inorganic materials 0.000 description 4
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 4
- 235000019796 monopotassium phosphate Nutrition 0.000 description 4
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 description 4
- 238000002360 preparation method Methods 0.000 description 4
- 235000017557 sodium bicarbonate Nutrition 0.000 description 4
- 229910000030 sodium bicarbonate Inorganic materials 0.000 description 4
- 239000011780 sodium chloride Substances 0.000 description 4
- 238000001179 sorption measurement Methods 0.000 description 4
- 238000005406 washing Methods 0.000 description 4
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 3
- 230000003466 anti-cipated effect Effects 0.000 description 3
- 239000004202 carbamide Substances 0.000 description 3
- 239000007822 coupling agent Substances 0.000 description 3
- 238000009792 diffusion process Methods 0.000 description 3
- 239000011654 magnesium acetate Substances 0.000 description 3
- 229940069446 magnesium acetate Drugs 0.000 description 3
- 150000004706 metal oxides Chemical class 0.000 description 3
- -1 A12O3 Substances 0.000 description 2
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
- 102000005158 Subtilisins Human genes 0.000 description 2
- 108010056079 Subtilisins Proteins 0.000 description 2
- GWEVSGVZZGPLCZ-UHFFFAOYSA-N Titan oxide Chemical compound O=[Ti]=O GWEVSGVZZGPLCZ-UHFFFAOYSA-N 0.000 description 2
- 235000012538 ammonium bicarbonate Nutrition 0.000 description 2
- 239000007864 aqueous solution Substances 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- HFACYLZERDEVSX-UHFFFAOYSA-N benzidine Chemical compound C1=CC(N)=CC=C1C1=CC=C(N)C=C1 HFACYLZERDEVSX-UHFFFAOYSA-N 0.000 description 2
- 230000001588 bifunctional effect Effects 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 239000012954 diazonium Substances 0.000 description 2
- 239000008103 glucose Substances 0.000 description 2
- 230000003100 immobilizing effect Effects 0.000 description 2
- UEGPKNKPLBYCNK-UHFFFAOYSA-L magnesium acetate Chemical compound [Mg+2].CC([O-])=O.CC([O-])=O UEGPKNKPLBYCNK-UHFFFAOYSA-L 0.000 description 2
- 235000011285 magnesium acetate Nutrition 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- 150000003839 salts Chemical class 0.000 description 2
- WVGMYVWWJSIIFJ-UHFFFAOYSA-N 2-methoxybenzenediazonium Chemical compound COC1=CC=CC=C1[N+]#N WVGMYVWWJSIIFJ-UHFFFAOYSA-N 0.000 description 1
- 238000012935 Averaging Methods 0.000 description 1
- 229930091371 Fructose Natural products 0.000 description 1
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 description 1
- 239000005715 Fructose Substances 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- BLRPTPMANUNPDV-UHFFFAOYSA-N Silane Chemical compound [SiH4] BLRPTPMANUNPDV-UHFFFAOYSA-N 0.000 description 1
- DWAQJAXMDSEUJJ-UHFFFAOYSA-M Sodium bisulfite Chemical compound [Na+].OS([O-])=O DWAQJAXMDSEUJJ-UHFFFAOYSA-M 0.000 description 1
- 206010042618 Surgical procedure repeated Diseases 0.000 description 1
- 239000012876 carrier material Substances 0.000 description 1
- 238000012412 chemical coupling Methods 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 150000003841 chloride salts Chemical class 0.000 description 1
- 150000001989 diazonium salts Chemical class 0.000 description 1
- IJGRMHOSHXDMSA-UHFFFAOYSA-O diazynium Chemical compound [NH+]#N IJGRMHOSHXDMSA-UHFFFAOYSA-O 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 239000006185 dispersion Substances 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 239000000706 filtrate Substances 0.000 description 1
- 238000010304 firing Methods 0.000 description 1
- 125000000524 functional group Chemical group 0.000 description 1
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 1
- 229910010272 inorganic material Inorganic materials 0.000 description 1
- 239000011147 inorganic material Substances 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 238000003801 milling Methods 0.000 description 1
- 229910003455 mixed metal oxide Inorganic materials 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 230000000737 periodic effect Effects 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 235000021317 phosphate Nutrition 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 235000018102 proteins Nutrition 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 239000000376 reactant Substances 0.000 description 1
- 239000011541 reaction mixture Substances 0.000 description 1
- 230000003252 repetitive effect Effects 0.000 description 1
- 229910000077 silane Inorganic materials 0.000 description 1
- 238000005245 sintering Methods 0.000 description 1
- 239000002002 slurry Substances 0.000 description 1
- 235000010267 sodium hydrogen sulphite Nutrition 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- YNJBWRMUSHSURL-UHFFFAOYSA-N trichloroacetic acid Chemical compound OC(=O)C(Cl)(Cl)Cl YNJBWRMUSHSURL-UHFFFAOYSA-N 0.000 description 1
- 125000001493 tyrosinyl group Chemical group [H]OC1=C([H])C([H])=C(C([H])=C1[H])C([H])([H])C([H])(N([H])[H])C(*)=O 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/14—Enzymes or microbial cells immobilised on or in an inorganic carrier
Landscapes
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Inorganic Chemistry (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US05/473,717 US3930951A (en) | 1974-05-28 | 1974-05-28 | Bonding enzymes to porous inorganic carriers |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA1037399A true CA1037399A (en) | 1978-08-29 |
Family
ID=23880695
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA219,586A Expired CA1037399A (en) | 1974-05-28 | 1975-02-07 | Method of bonding enzymes to porous inorganic carriers |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US3930951A ( ) |
| JP (1) | JPS5819274B2 ( ) |
| CA (1) | CA1037399A ( ) |
| DE (1) | DE2514225C2 ( ) |
| FR (1) | FR2273008B1 ( ) |
| GB (1) | GB1457993A ( ) |
| IT (1) | IT1038178B ( ) |
Families Citing this family (20)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3983000A (en) * | 1976-04-01 | 1976-09-28 | Corning Glass Works | Bonding proteins to inorganic supports |
| JPS52120190A (en) * | 1976-04-02 | 1977-10-08 | Cpc International Inc | Fixing method of glucose isomerase and cotinuous isomerization of glucose |
| US4107287A (en) * | 1976-04-12 | 1978-08-15 | University Of Pennsylvania | GC Antigen-specific immunochemical indicator reagent and method for preparing same |
| US4072566A (en) * | 1976-09-27 | 1978-02-07 | Corning Glass Works | Immobilized biologically active proteins |
| DE2726188C2 (de) * | 1977-06-10 | 1979-05-10 | Kali-Chemie Ag, 3000 Hannover | Verfahren zur Herstellung eines wasserunlöslichen Enzympräparats |
| GB2004300B (en) * | 1977-09-14 | 1982-08-04 | Corning Glass Works | High surface low volume biomass composites |
| US4206286A (en) * | 1977-11-14 | 1980-06-03 | Technicon Instruments Corporation | Immobilization of proteins on inorganic supports |
| US4204040A (en) * | 1977-11-18 | 1980-05-20 | Technicon Instruments Corporation | Copolymerization of proteins on an inorganic support |
| JPS5849537B2 (ja) * | 1980-03-18 | 1983-11-05 | 三菱化学株式会社 | 尿素の製造方法 |
| US4748121A (en) * | 1984-11-30 | 1988-05-31 | Ppg Industries, Inc. | Porous glass fibers with immobilized biochemically active material |
| US4861714A (en) * | 1985-04-04 | 1989-08-29 | Verax Corporation | Weighted collagen microsponge for immobilizing bioactive material |
| US5100783A (en) * | 1985-05-10 | 1992-03-31 | Verax Corporation | Weighted microsponge for immobilizing bioactive material |
| IL93134A (en) * | 1990-01-23 | 1997-11-20 | Yissum Res Dev Co | Doped sol-gel glasses for obtaining chemical interactions |
| US5371018A (en) * | 1990-01-23 | 1994-12-06 | Yissum, Research Development Company Of The Hebrew University Of Jerusalem | Qualitative and quantitative processes for reactive chemicals in liquids using doped sol-gel glasses |
| JPH0536930U (ja) * | 1991-10-18 | 1993-05-18 | アルプス電気株式会社 | 平衡型発振器の共振回路 |
| JP2735458B2 (ja) * | 1993-06-18 | 1998-04-02 | 日本碍子株式会社 | 生体触媒用担体 |
| US7008599B1 (en) * | 1999-05-10 | 2006-03-07 | Smithkline Beecham Corporation | High throughput crystal form screening workstation and method of use |
| US20110218365A1 (en) * | 2008-09-05 | 2011-09-08 | Cobalt Technologies, Inc. | Engineered light-emitting reporter genes and methods of use |
| CN102482690A (zh) | 2009-06-26 | 2012-05-30 | 钴技术有限公司 | 生物产物生产的集成系统和方法 |
| CN103387975A (zh) | 2012-05-11 | 2013-11-13 | 上海天伟生物制药有限公司 | 一种固定化环脂肽酰基转移酶及其制备方法和用途 |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US2968635A (en) * | 1958-09-19 | 1961-01-17 | Universal Oil Prod Co | Activated alumina and the use thereof |
| US3666627A (en) * | 1968-10-14 | 1972-05-30 | Corning Glass Works | Method of stabilizing enzymes |
| US3669841A (en) * | 1970-02-11 | 1972-06-13 | Monsanto Co | Attachment of enzymes to siliceous materials |
| US3705084A (en) * | 1970-03-18 | 1972-12-05 | Monsanto Co | Macroporous enzyme reactor |
| US3783101A (en) * | 1972-02-17 | 1974-01-01 | Corning Glass Works | Enzymes bound to carriers having a metal oxide surface layer |
| US3804719A (en) * | 1972-08-07 | 1974-04-16 | Corning Glass Works | Adsorbing and crosslinking enzymes within the pores of porous glass |
-
1974
- 1974-05-28 US US05/473,717 patent/US3930951A/en not_active Expired - Lifetime
-
1975
- 1975-02-07 CA CA219,586A patent/CA1037399A/en not_active Expired
- 1975-04-01 DE DE2514225A patent/DE2514225C2/de not_active Expired
- 1975-05-16 IT IT23395/75A patent/IT1038178B/it active
- 1975-05-21 GB GB2177475A patent/GB1457993A/en not_active Expired
- 1975-05-26 JP JP50062845A patent/JPS5819274B2/ja not_active Expired
- 1975-05-27 FR FR7516419A patent/FR2273008B1/fr not_active Expired
Also Published As
| Publication number | Publication date |
|---|---|
| US3930951A (en) | 1976-01-06 |
| IT1038178B (it) | 1979-11-20 |
| FR2273008B1 ( ) | 1979-07-13 |
| JPS5819274B2 (ja) | 1983-04-16 |
| DE2514225C2 (de) | 1985-08-08 |
| JPS511691A (en) | 1976-01-08 |
| DE2514225A1 (de) | 1975-12-11 |
| FR2273008A1 ( ) | 1975-12-26 |
| GB1457993A (en) | 1976-12-08 |
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