DE2513259A1 - Verfahren zum anreichern von mutanten in hefekulturen - Google Patents
Verfahren zum anreichern von mutanten in hefekulturenInfo
- Publication number
- DE2513259A1 DE2513259A1 DE19752513259 DE2513259A DE2513259A1 DE 2513259 A1 DE2513259 A1 DE 2513259A1 DE 19752513259 DE19752513259 DE 19752513259 DE 2513259 A DE2513259 A DE 2513259A DE 2513259 A1 DE2513259 A1 DE 2513259A1
- Authority
- DE
- Germany
- Prior art keywords
- medium
- glucose
- mutants
- cells
- fluoro
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000000034 method Methods 0.000 title claims description 23
- 240000004808 Saccharomyces cerevisiae Species 0.000 title claims description 10
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims description 29
- 230000012010 growth Effects 0.000 claims description 22
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 19
- 239000008103 glucose Substances 0.000 claims description 19
- GHASVSINZRGABV-UHFFFAOYSA-N Fluorouracil Chemical compound FC1=CNC(=O)NC1=O GHASVSINZRGABV-UHFFFAOYSA-N 0.000 claims description 18
- 229960002949 fluorouracil Drugs 0.000 claims description 18
- 235000011187 glycerol Nutrition 0.000 claims description 12
- 230000003505 mutagenic effect Effects 0.000 claims description 6
- 239000003471 mutagenic agent Substances 0.000 claims description 5
- 235000015097 nutrients Nutrition 0.000 claims description 5
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 claims description 4
- 231100000707 mutagenic chemical Toxicity 0.000 claims description 4
- 239000003795 chemical substances by application Substances 0.000 claims description 2
- 235000013305 food Nutrition 0.000 claims description 2
- 230000006798 recombination Effects 0.000 claims description 2
- 238000005215 recombination Methods 0.000 claims description 2
- 230000000392 somatic effect Effects 0.000 claims description 2
- 241000894007 species Species 0.000 claims description 2
- 239000002609 medium Substances 0.000 description 34
- 210000004027 cell Anatomy 0.000 description 29
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 description 8
- 230000006698 induction Effects 0.000 description 7
- 230000035772 mutation Effects 0.000 description 7
- 229920001817 Agar Polymers 0.000 description 5
- 239000008272 agar Substances 0.000 description 5
- 150000001413 amino acids Chemical class 0.000 description 5
- 238000011534 incubation Methods 0.000 description 4
- 238000011081 inoculation Methods 0.000 description 4
- 230000004083 survival effect Effects 0.000 description 4
- 230000032823 cell division Effects 0.000 description 3
- 239000001963 growth medium Substances 0.000 description 3
- 230000002401 inhibitory effect Effects 0.000 description 3
- 230000005764 inhibitory process Effects 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 230000000241 respiratory effect Effects 0.000 description 3
- 238000004448 titration Methods 0.000 description 3
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 2
- PYOHODCEOHCZBM-RYUDHWBXSA-N Phe-Met Chemical compound CSCC[C@@H](C(O)=O)NC(=O)[C@@H](N)CC1=CC=CC=C1 PYOHODCEOHCZBM-RYUDHWBXSA-N 0.000 description 2
- 230000003698 anagen phase Effects 0.000 description 2
- 229910052799 carbon Inorganic materials 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000002068 genetic effect Effects 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 210000003470 mitochondria Anatomy 0.000 description 2
- 231100000219 mutagenic Toxicity 0.000 description 2
- 230000036438 mutation frequency Effects 0.000 description 2
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 238000002255 vaccination Methods 0.000 description 2
- 210000005253 yeast cell Anatomy 0.000 description 2
- 229930182555 Penicillin Natural products 0.000 description 1
- JGSARLDLIJGVTE-MBNYWOFBSA-N Penicillin G Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-MBNYWOFBSA-N 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 238000007792 addition Methods 0.000 description 1
- 230000003466 anti-cipated effect Effects 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
- 239000006285 cell suspension Substances 0.000 description 1
- 210000000805 cytoplasm Anatomy 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 210000001840 diploid cell Anatomy 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 239000003292 glue Substances 0.000 description 1
- 230000009036 growth inhibition Effects 0.000 description 1
- SMVZDCVZXKQERC-UHFFFAOYSA-N guanidine;nitrous acid Chemical compound ON=O.NC(N)=N SMVZDCVZXKQERC-UHFFFAOYSA-N 0.000 description 1
- 230000001678 irradiating effect Effects 0.000 description 1
- 238000009630 liquid culture Methods 0.000 description 1
- 231100000350 mutagenesis Toxicity 0.000 description 1
- 238000002703 mutagenesis Methods 0.000 description 1
- 230000008518 non respiratory effect Effects 0.000 description 1
- 229940049954 penicillin Drugs 0.000 description 1
- 230000029058 respiratory gaseous exchange Effects 0.000 description 1
- 230000000284 resting effect Effects 0.000 description 1
- 230000000087 stabilizing effect Effects 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/01—Preparation of mutants without inserting foreign genetic material therein; Screening processes therefor
Landscapes
- Genetics & Genomics (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| IT49767/74A IT1003930B (it) | 1974-03-27 | 1974-03-27 | Procedimento di arricchimento in mu tanti di colture di microorganismi presente in lieviti adibiti ad uso alimentar |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DE2513259A1 true DE2513259A1 (de) | 1975-10-09 |
Family
ID=11271513
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE19752513259 Pending DE2513259A1 (de) | 1974-03-27 | 1975-03-26 | Verfahren zum anreichern von mutanten in hefekulturen |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US3954536A (OSRAM) |
| JP (1) | JPS517175A (OSRAM) |
| BE (1) | BE826970A (OSRAM) |
| DE (1) | DE2513259A1 (OSRAM) |
| FR (1) | FR2265860B3 (OSRAM) |
| GB (1) | GB1500163A (OSRAM) |
| IT (1) | IT1003930B (OSRAM) |
| NL (1) | NL7503601A (OSRAM) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB1590830A (en) * | 1976-12-24 | 1981-06-10 | Lesaffre & Cie | Strains of yeast for bread-making and a process for the obtention thereof |
| US4511656A (en) * | 1981-05-15 | 1985-04-16 | Purdue Research Foundation | Direct fermentation of D-xylose to ethanol by a xylose-fermenting yeast mutant |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE1442304A1 (de) * | 1962-08-08 | 1970-01-15 | Takeda Chemical Industries Ltd | Verfahren zur Herstellung von Purinderivaten |
-
1974
- 1974-03-27 IT IT49767/74A patent/IT1003930B/it active
-
1975
- 1975-03-19 US US05/559,633 patent/US3954536A/en not_active Expired - Lifetime
- 1975-03-21 BE BE154573A patent/BE826970A/xx unknown
- 1975-03-25 GB GB12495/75A patent/GB1500163A/en not_active Expired
- 1975-03-25 NL NL7503601A patent/NL7503601A/xx not_active Application Discontinuation
- 1975-03-25 JP JP50036003A patent/JPS517175A/ja active Pending
- 1975-03-26 DE DE19752513259 patent/DE2513259A1/de active Pending
- 1975-03-26 FR FR7509491A patent/FR2265860B3/fr not_active Expired
Also Published As
| Publication number | Publication date |
|---|---|
| BE826970A (fr) | 1975-07-16 |
| JPS517175A (en) | 1976-01-21 |
| FR2265860B3 (OSRAM) | 1977-12-02 |
| US3954536A (en) | 1976-05-04 |
| NL7503601A (nl) | 1975-09-30 |
| GB1500163A (en) | 1978-02-08 |
| FR2265860A1 (OSRAM) | 1975-10-24 |
| IT1003930B (it) | 1976-06-10 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| OHJ | Non-payment of the annual fee |