DE2330555A1 - Verfahren zur untersuchung von serumthyroxin, durch das aenderungen des gehaltes an thyroxin-bindendem globulin durch physiologische bedingungen kompensiert werden - Google Patents
Verfahren zur untersuchung von serumthyroxin, durch das aenderungen des gehaltes an thyroxin-bindendem globulin durch physiologische bedingungen kompensiert werdenInfo
- Publication number
- DE2330555A1 DE2330555A1 DE19732330555 DE2330555A DE2330555A1 DE 2330555 A1 DE2330555 A1 DE 2330555A1 DE 19732330555 DE19732330555 DE 19732330555 DE 2330555 A DE2330555 A DE 2330555A DE 2330555 A1 DE2330555 A1 DE 2330555A1
- Authority
- DE
- Germany
- Prior art keywords
- thyroxine
- serum
- serum sample
- binding
- binding globulin
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- XUIIKFGFIJCVMT-UHFFFAOYSA-N thyroxine-binding globulin Natural products IC1=CC(CC([NH3+])C([O-])=O)=CC(I)=C1OC1=CC(I)=C(O)C(I)=C1 XUIIKFGFIJCVMT-UHFFFAOYSA-N 0.000 title claims abstract description 117
- 210000002966 serum Anatomy 0.000 title claims abstract description 84
- 229940034208 thyroxine Drugs 0.000 title claims abstract description 84
- XUIIKFGFIJCVMT-GFCCVEGCSA-N D-thyroxine Chemical compound IC1=CC(C[C@@H](N)C(O)=O)=CC(I)=C1OC1=CC(I)=C(O)C(I)=C1 XUIIKFGFIJCVMT-GFCCVEGCSA-N 0.000 title claims abstract description 78
- 108010000259 Thyroxine-Binding Globulin Proteins 0.000 claims abstract description 44
- 238000000034 method Methods 0.000 claims abstract description 23
- 239000011347 resin Substances 0.000 claims abstract description 20
- 229920005989 resin Polymers 0.000 claims abstract description 20
- NWUYHJFMYQTDRP-UHFFFAOYSA-N 1,2-bis(ethenyl)benzene;1-ethenyl-2-ethylbenzene;styrene Chemical compound C=CC1=CC=CC=C1.CCC1=CC=CC=C1C=C.C=CC1=CC=CC=C1C=C NWUYHJFMYQTDRP-UHFFFAOYSA-N 0.000 claims abstract description 8
- 239000003456 ion exchange resin Substances 0.000 claims abstract description 8
- 229920003303 ion-exchange polymer Polymers 0.000 claims abstract description 8
- 230000004962 physiological condition Effects 0.000 claims abstract description 7
- 230000002159 abnormal effect Effects 0.000 claims abstract description 6
- 239000000700 radioactive tracer Substances 0.000 claims abstract description 6
- 230000009137 competitive binding Effects 0.000 claims abstract 2
- 102000002248 Thyroxine-Binding Globulin Human genes 0.000 claims description 39
- 230000027455 binding Effects 0.000 claims description 26
- 239000000203 mixture Substances 0.000 claims description 11
- 230000002285 radioactive effect Effects 0.000 claims description 6
- 229920006395 saturated elastomer Polymers 0.000 claims description 6
- 239000003957 anion exchange resin Substances 0.000 claims description 4
- 102000009488 Thyroxine-Binding Proteins Human genes 0.000 claims description 2
- 108010048889 Thyroxine-Binding Proteins Proteins 0.000 claims description 2
- 230000037406 food intake Effects 0.000 claims description 2
- 239000002904 solvent Substances 0.000 claims description 2
- ZCYVEMRRCGMTRW-UHFFFAOYSA-N 7553-56-2 Chemical compound [I] ZCYVEMRRCGMTRW-UHFFFAOYSA-N 0.000 claims 3
- 229910052740 iodine Inorganic materials 0.000 claims 3
- 239000011630 iodine Substances 0.000 claims 3
- 102000006395 Globulins Human genes 0.000 claims 2
- 108010044091 Globulins Proteins 0.000 claims 2
- 238000012360 testing method Methods 0.000 abstract description 17
- 239000000262 estrogen Substances 0.000 abstract description 11
- 230000035935 pregnancy Effects 0.000 abstract description 11
- 238000002560 therapeutic procedure Methods 0.000 abstract description 8
- 238000002156 mixing Methods 0.000 abstract description 2
- 102100028709 Thyroxine-binding globulin Human genes 0.000 abstract 5
- 230000004075 alteration Effects 0.000 abstract 1
- 239000000523 sample Substances 0.000 description 15
- 102000004169 proteins and genes Human genes 0.000 description 11
- 108090000623 proteins and genes Proteins 0.000 description 11
- 229940011871 estrogen Drugs 0.000 description 10
- 206010020850 Hyperthyroidism Diseases 0.000 description 9
- AUYYCJSJGJYCDS-LBPRGKRZSA-N Thyrolar Chemical compound IC1=CC(C[C@H](N)C(O)=O)=CC(I)=C1OC1=CC=C(O)C(I)=C1 AUYYCJSJGJYCDS-LBPRGKRZSA-N 0.000 description 9
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 8
- 229940088597 hormone Drugs 0.000 description 6
- 239000005556 hormone Substances 0.000 description 6
- 230000002860 competitive effect Effects 0.000 description 5
- 230000000694 effects Effects 0.000 description 5
- 208000003532 hypothyroidism Diseases 0.000 description 5
- 230000002989 hypothyroidism Effects 0.000 description 5
- 210000001685 thyroid gland Anatomy 0.000 description 5
- 230000009977 dual effect Effects 0.000 description 4
- 238000000605 extraction Methods 0.000 description 3
- 238000011534 incubation Methods 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 229940127234 oral contraceptive Drugs 0.000 description 3
- 239000003539 oral contraceptive agent Substances 0.000 description 3
- 230000001575 pathological effect Effects 0.000 description 3
- 238000010998 test method Methods 0.000 description 3
- 102000004506 Blood Proteins Human genes 0.000 description 2
- 108010017384 Blood Proteins Proteins 0.000 description 2
- LRHPLDYGYMQRHN-UHFFFAOYSA-N N-Butanol Chemical compound CCCCO LRHPLDYGYMQRHN-UHFFFAOYSA-N 0.000 description 2
- 125000000129 anionic group Chemical group 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- 239000008280 blood Substances 0.000 description 2
- 230000000052 comparative effect Effects 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 229940079593 drug Drugs 0.000 description 2
- 238000002523 gelfiltration Methods 0.000 description 2
- 238000000338 in vitro Methods 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 238000002483 medication Methods 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 102000009027 Albumins Human genes 0.000 description 1
- 108010088751 Albumins Proteins 0.000 description 1
- 241000736355 Euthyroides Species 0.000 description 1
- XUIIKFGFIJCVMT-LBPRGKRZSA-N L-thyroxine Chemical compound IC1=CC(C[C@H]([NH3+])C([O-])=O)=CC(I)=C1OC1=CC(I)=C(O)C(I)=C1 XUIIKFGFIJCVMT-LBPRGKRZSA-N 0.000 description 1
- 206010029164 Nephrotic syndrome Diseases 0.000 description 1
- 239000004743 Polypropylene Substances 0.000 description 1
- 229920005830 Polyurethane Foam Polymers 0.000 description 1
- 102000007584 Prealbumin Human genes 0.000 description 1
- 108010071690 Prealbumin Proteins 0.000 description 1
- 208000007536 Thrombosis Diseases 0.000 description 1
- 230000005856 abnormality Effects 0.000 description 1
- 239000003098 androgen Substances 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000009134 cell regulation Effects 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 238000003759 clinical diagnosis Methods 0.000 description 1
- 239000013068 control sample Substances 0.000 description 1
- 238000012937 correction Methods 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 238000010494 dissociation reaction Methods 0.000 description 1
- 230000005593 dissociations Effects 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 210000004907 gland Anatomy 0.000 description 1
- 229950008325 levothyroxine Drugs 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 208000009928 nephrosis Diseases 0.000 description 1
- 238000010606 normalization Methods 0.000 description 1
- 231100000915 pathological change Toxicity 0.000 description 1
- 230000036285 pathological change Effects 0.000 description 1
- -1 polypropylene Polymers 0.000 description 1
- 229920001155 polypropylene Polymers 0.000 description 1
- 239000011496 polyurethane foam Substances 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 239000013049 sediment Substances 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/74—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving hormones or other non-cytokine intercellular protein regulatory factors such as growth factors, including receptors to hormones and growth factors
- G01N33/78—Thyroid gland hormones, e.g. T3, T4, TBH, TBG or their receptors
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Molecular Biology (AREA)
- Engineering & Computer Science (AREA)
- Endocrinology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Biotechnology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US26351472A | 1972-06-16 | 1972-06-16 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DE2330555A1 true DE2330555A1 (de) | 1974-01-03 |
Family
ID=23002079
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE19732330555 Pending DE2330555A1 (de) | 1972-06-16 | 1973-06-15 | Verfahren zur untersuchung von serumthyroxin, durch das aenderungen des gehaltes an thyroxin-bindendem globulin durch physiologische bedingungen kompensiert werden |
Country Status (4)
| Country | Link |
|---|---|
| JP (1) | JPS4964492A (enExample) |
| AU (1) | AU5701473A (enExample) |
| CA (1) | CA1015252A (enExample) |
| DE (1) | DE2330555A1 (enExample) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0002544A1 (en) * | 1977-12-16 | 1979-06-27 | Mallinckrodt Diagnostica (Holland) B.V. | Radioimmunological method of determining the thyroid function by an in vitro test in blood serum |
-
1973
- 1973-03-30 CA CA167,585A patent/CA1015252A/en not_active Expired
- 1973-06-08 JP JP6395773A patent/JPS4964492A/ja active Pending
- 1973-06-15 DE DE19732330555 patent/DE2330555A1/de active Pending
- 1973-06-18 AU AU57014/73A patent/AU5701473A/en not_active Expired
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0002544A1 (en) * | 1977-12-16 | 1979-06-27 | Mallinckrodt Diagnostica (Holland) B.V. | Radioimmunological method of determining the thyroid function by an in vitro test in blood serum |
Also Published As
| Publication number | Publication date |
|---|---|
| JPS4964492A (enExample) | 1974-06-21 |
| CA1015252A (en) | 1977-08-09 |
| AU5701473A (en) | 1974-12-19 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| OHA | Expiration of time for request for examination |