DE2224132C2 - Verfahren und Reagenz zur Bestimmung von Cholesterin - Google Patents
Verfahren und Reagenz zur Bestimmung von CholesterinInfo
- Publication number
- DE2224132C2 DE2224132C2 DE19722224132 DE2224132A DE2224132C2 DE 2224132 C2 DE2224132 C2 DE 2224132C2 DE 19722224132 DE19722224132 DE 19722224132 DE 2224132 A DE2224132 A DE 2224132A DE 2224132 C2 DE2224132 C2 DE 2224132C2
- Authority
- DE
- Germany
- Prior art keywords
- cholesterol
- determination
- reagent
- cholestenone
- buffer
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 title claims description 90
- 235000012000 cholesterol Nutrition 0.000 title claims description 44
- 239000003153 chemical reaction reagent Substances 0.000 title claims description 22
- 238000000034 method Methods 0.000 title claims description 22
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims description 21
- 108010089254 Cholesterol oxidase Proteins 0.000 claims description 16
- YRKCREAYFQTBPV-UHFFFAOYSA-N acetylacetone Chemical compound CC(=O)CC(C)=O YRKCREAYFQTBPV-UHFFFAOYSA-N 0.000 claims description 16
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 claims description 11
- GGCLNOIGPMGLDB-GYKMGIIDSA-N cholest-5-en-3-one Chemical compound C1C=C2CC(=O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 GGCLNOIGPMGLDB-GYKMGIIDSA-N 0.000 claims description 11
- NYOXRYYXRWJDKP-UHFFFAOYSA-N cholestenone Natural products C1CC2=CC(=O)CCC2(C)C2C1C1CCC(C(C)CCCC(C)C)C1(C)CC2 NYOXRYYXRWJDKP-UHFFFAOYSA-N 0.000 claims description 11
- 102000016938 Catalase Human genes 0.000 claims description 9
- 108010053835 Catalase Proteins 0.000 claims description 9
- 239000000872 buffer Substances 0.000 claims description 9
- 102000003992 Peroxidases Human genes 0.000 claims description 8
- 230000036284 oxygen consumption Effects 0.000 claims description 8
- 108040007629 peroxidase activity proteins Proteins 0.000 claims description 8
- HORQAOAYAYGIBM-UHFFFAOYSA-N 2,4-dinitrophenylhydrazine Chemical compound NNC1=CC=C([N+]([O-])=O)C=C1[N+]([O-])=O HORQAOAYAYGIBM-UHFFFAOYSA-N 0.000 claims description 6
- 238000007127 saponification reaction Methods 0.000 claims description 6
- 239000012736 aqueous medium Substances 0.000 claims description 4
- 125000000468 ketone group Chemical group 0.000 claims description 4
- 239000004094 surface-active agent Substances 0.000 claims description 4
- LSNNMFCWUKXFEE-UHFFFAOYSA-M Bisulfite Chemical compound OS([O-])=O LSNNMFCWUKXFEE-UHFFFAOYSA-M 0.000 claims description 2
- 229930194542 Keto Natural products 0.000 claims 1
- 239000003795 chemical substances by application Substances 0.000 claims 1
- 150000007857 hydrazones Chemical class 0.000 claims 1
- 238000006243 chemical reaction Methods 0.000 description 13
- 239000000243 solution Substances 0.000 description 13
- 210000002966 serum Anatomy 0.000 description 10
- 238000005259 measurement Methods 0.000 description 9
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 8
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 5
- 238000011088 calibration curve Methods 0.000 description 4
- WFDIJRYMOXRFFG-UHFFFAOYSA-N Acetic anhydride Chemical compound CC(=O)OC(C)=O WFDIJRYMOXRFFG-UHFFFAOYSA-N 0.000 description 3
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 description 3
- 208000035150 Hypercholesterolemia Diseases 0.000 description 3
- 230000001476 alcoholic effect Effects 0.000 description 3
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 3
- 230000002255 enzymatic effect Effects 0.000 description 3
- 239000001301 oxygen Substances 0.000 description 3
- 229910052760 oxygen Inorganic materials 0.000 description 3
- NLKNQRATVPKPDG-UHFFFAOYSA-M potassium iodide Chemical compound [K+].[I-] NLKNQRATVPKPDG-UHFFFAOYSA-M 0.000 description 3
- 239000012086 standard solution Substances 0.000 description 3
- 150000003431 steroids Chemical class 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 108090000371 Esterases Proteins 0.000 description 2
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 238000002835 absorbance Methods 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 235000019256 formaldehyde Nutrition 0.000 description 2
- 150000002429 hydrazines Chemical class 0.000 description 2
- -1 hypercholesterolemia Chemical compound 0.000 description 2
- 210000004185 liver Anatomy 0.000 description 2
- CKFGINPQOCXMAZ-UHFFFAOYSA-N methanediol Chemical compound OCO CKFGINPQOCXMAZ-UHFFFAOYSA-N 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 239000008057 potassium phosphate buffer Substances 0.000 description 2
- QYIXCDOBOSTCEI-QCYZZNICSA-N (5alpha)-cholestan-3beta-ol Chemical compound C([C@@H]1CC2)[C@@H](O)CC[C@]1(C)[C@@H]1[C@@H]2[C@@H]2CC[C@H]([C@H](C)CCCC(C)C)[C@@]2(C)CC1 QYIXCDOBOSTCEI-QCYZZNICSA-N 0.000 description 1
- 206010003210 Arteriosclerosis Diseases 0.000 description 1
- 201000001320 Atherosclerosis Diseases 0.000 description 1
- 208000008439 Biliary Liver Cirrhosis Diseases 0.000 description 1
- 206010004659 Biliary cirrhosis Diseases 0.000 description 1
- 206010052895 Coronary artery insufficiency Diseases 0.000 description 1
- 206010014476 Elevated cholesterol Diseases 0.000 description 1
- 241000243251 Hydra Species 0.000 description 1
- 206010029164 Nephrotic syndrome Diseases 0.000 description 1
- 108010055297 Sterol Esterase Proteins 0.000 description 1
- 102000000019 Sterol Esterase Human genes 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- QYIXCDOBOSTCEI-UHFFFAOYSA-N alpha-cholestanol Natural products C1CC2CC(O)CCC2(C)C2C1C1CCC(C(C)CCCC(C)C)C1(C)CC2 QYIXCDOBOSTCEI-UHFFFAOYSA-N 0.000 description 1
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 1
- QGAVSDVURUSLQK-UHFFFAOYSA-N ammonium heptamolybdate Chemical compound N.N.N.N.N.N.O.O.O.O.O.O.O.O.O.O.O.O.O.O.O.O.O.O.O.O.O.O.O.O.[Mo].[Mo].[Mo].[Mo].[Mo].[Mo].[Mo] QGAVSDVURUSLQK-UHFFFAOYSA-N 0.000 description 1
- 208000011775 arteriosclerosis disease Diseases 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000010839 body fluid Substances 0.000 description 1
- 239000003518 caustics Substances 0.000 description 1
- FLKYBGKDCCEQQM-WYUVZMMLSA-M cefazolin sodium Chemical compound [Na+].S1C(C)=NN=C1SCC1=C(C([O-])=O)N2C(=O)[C@@H](NC(=O)CN3N=NN=C3)[C@H]2SC1 FLKYBGKDCCEQQM-WYUVZMMLSA-M 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 206010012601 diabetes mellitus Diseases 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- MNNHAPBLZZVQHP-UHFFFAOYSA-N diammonium hydrogen phosphate Chemical compound [NH4+].[NH4+].OP([O-])([O-])=O MNNHAPBLZZVQHP-UHFFFAOYSA-N 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000006911 enzymatic reaction Methods 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 239000000945 filler Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 238000004817 gas chromatography Methods 0.000 description 1
- 208000003532 hypothyroidism Diseases 0.000 description 1
- 230000002989 hypothyroidism Effects 0.000 description 1
- QRXWMOHMRWLFEY-UHFFFAOYSA-N isoniazide Chemical compound NNC(=O)C1=CC=NC=C1 QRXWMOHMRWLFEY-UHFFFAOYSA-N 0.000 description 1
- 208000019423 liver disease Diseases 0.000 description 1
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 1
- 235000019341 magnesium sulphate Nutrition 0.000 description 1
- 230000028161 membrane depolarization Effects 0.000 description 1
- 208000010125 myocardial infarction Diseases 0.000 description 1
- 210000000496 pancreas Anatomy 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 229920006395 saturated elastomer Polymers 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 239000003381 stabilizer Substances 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- QAOWNCQODCNURD-UHFFFAOYSA-N sulfuric acid Substances OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/60—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving cholesterol
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Priority Applications (32)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE2264847A DE2264847C2 (de) | 1972-05-17 | 1972-05-17 | Verfahren und Reagenz zur Bestimmung von Cholesterin |
| DE19722224132 DE2224132C2 (de) | 1972-05-17 | 1972-05-17 | Verfahren und Reagenz zur Bestimmung von Cholesterin |
| AR247906A AR195000A1 (es) | 1972-05-17 | 1973-05-08 | Procedimiento para la determinacion de colesterol |
| DK255673A DK145234C (da) | 1972-05-17 | 1973-05-09 | Fremgangsmaade og reagens til kvantitativ bestemmelse af cholesterol |
| CH163077A CH610108A5 (enrdf_load_stackoverflow) | 1972-05-17 | 1973-05-14 | |
| CH681273A CH589295A5 (enrdf_load_stackoverflow) | 1972-05-17 | 1973-05-14 | |
| HUBO1435A HU169171B (enrdf_load_stackoverflow) | 1972-05-17 | 1973-05-15 | |
| ZA733258A ZA733258B (en) | 1972-05-17 | 1973-05-15 | Enzymatic determination of cholesterol |
| NL7306738A NL181282C (nl) | 1972-05-17 | 1973-05-15 | Werkwijze en reagentiacombinatie voor het bepalen van veresterd en vrij cholesterol in vloeistoffen. |
| US05/360,515 US4212938A (en) | 1972-05-17 | 1973-05-15 | Reagent and method for the determination of cholesterol |
| AT426273A AT324580B (de) | 1972-05-17 | 1973-05-15 | Verfahren und reagens zur bestimmung von cholesterin |
| BE131117A BE799543A (fr) | 1972-05-17 | 1973-05-15 | Procede et reactif pour le dosage du cholesterol, |
| DD170832A DD103324A5 (enrdf_load_stackoverflow) | 1972-05-17 | 1973-05-15 | |
| GB2308273A GB1429525A (en) | 1972-05-17 | 1973-05-15 | Process and reagent for the quantitative determination of cholesterol |
| IT24188/73A IT994863B (it) | 1972-05-17 | 1973-05-16 | Reagente per la determinazione della colesterina |
| SE7306915A SE387175B (sv) | 1972-05-17 | 1973-05-16 | Forfarande och reagens for bestemning av kolesterol |
| FI1573/73A FI53507C (fi) | 1972-05-17 | 1973-05-16 | Foerfarande och reagens foer bestaemning av kolesterol |
| IL42289A IL42289A (en) | 1972-05-17 | 1973-05-16 | Process and notice for cholesterol determination |
| YU01295/73A YU129573A (en) | 1972-05-17 | 1973-05-16 | Process for the determination of cholesterine |
| CS3543A CS163299B2 (enrdf_load_stackoverflow) | 1972-05-17 | 1973-05-17 | |
| JP48055157A JPS4962193A (enrdf_load_stackoverflow) | 1972-05-17 | 1973-05-17 | |
| SU731928101A SU664536A3 (ru) | 1972-05-17 | 1973-05-17 | Реактив дл определени холестерина |
| MX143632A MX153761A (es) | 1972-05-17 | 1973-05-17 | Procedimiento para determinar la colesterina(colesterol)presente en productos industriales y material biologico |
| AU55861/73A AU484754B2 (en) | 1972-05-17 | 1973-05-17 | Process and reagent forthe determination of cholesterol |
| FR7317898A FR2191743A5 (enrdf_load_stackoverflow) | 1972-05-17 | 1973-05-17 | |
| SU731981860A SU637097A3 (ru) | 1972-05-17 | 1973-12-07 | Способ определени свободного св занного холестерина в биологических жидкост х |
| SU762325958A SU622424A3 (ru) | 1972-05-17 | 1976-02-24 | Реактив дл определени холестерина |
| JP9956576A JPS52106856A (en) | 1972-05-17 | 1976-08-20 | Quantitative method of cholesterol and reagent utilized in said method |
| JP2586877A JPS53111791A (en) | 1972-05-17 | 1977-03-09 | Reagent for determination of cholesterin |
| JP2586977A JPS53111792A (en) | 1972-05-17 | 1977-03-09 | Quantitative determination of cholesterin |
| JP61127423A JPS62275700A (ja) | 1972-05-17 | 1986-05-30 | 血清中のコレステリンを測定するための試薬 |
| JP61127422A JPS62272999A (ja) | 1972-05-17 | 1986-05-30 | 血清中のコレステリンを測定するための試薬 |
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE19722224132 DE2224132C2 (de) | 1972-05-17 | 1972-05-17 | Verfahren und Reagenz zur Bestimmung von Cholesterin |
| DE2264847A DE2264847C2 (de) | 1972-05-17 | 1972-05-17 | Verfahren und Reagenz zur Bestimmung von Cholesterin |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| DE2224132A1 DE2224132A1 (enrdf_load_stackoverflow) | 1973-11-22 |
| DE2224132B1 DE2224132B1 (de) | 1973-11-22 |
| DE2224132C2 true DE2224132C2 (de) | 1980-04-03 |
Family
ID=25763268
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE19722224132 Expired DE2224132C2 (de) | 1972-05-17 | 1972-05-17 | Verfahren und Reagenz zur Bestimmung von Cholesterin |
| DE2264847A Expired DE2264847C2 (de) | 1972-05-17 | 1972-05-17 | Verfahren und Reagenz zur Bestimmung von Cholesterin |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE2264847A Expired DE2264847C2 (de) | 1972-05-17 | 1972-05-17 | Verfahren und Reagenz zur Bestimmung von Cholesterin |
Country Status (7)
| Country | Link |
|---|---|
| AT (1) | AT324580B (enrdf_load_stackoverflow) |
| BE (1) | BE799543A (enrdf_load_stackoverflow) |
| CH (1) | CH589295A5 (enrdf_load_stackoverflow) |
| DE (2) | DE2224132C2 (enrdf_load_stackoverflow) |
| FR (1) | FR2191743A5 (enrdf_load_stackoverflow) |
| GB (1) | GB1429525A (enrdf_load_stackoverflow) |
| NL (1) | NL181282C (enrdf_load_stackoverflow) |
Families Citing this family (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4186251A (en) * | 1973-03-01 | 1980-01-29 | Miles Laboratories, Inc. | Composition and method for determination of cholesterol |
| US4164448A (en) * | 1973-12-07 | 1979-08-14 | Boehringer Mannheim Gmbh | Activation of cholesterol oxidase for cholesterol assay |
| US3869349A (en) * | 1974-03-25 | 1975-03-04 | Eastman Kodak Co | Method for the enzymatic hydrolysis of cholesterol esters |
| CA1056282A (en) * | 1974-03-25 | 1979-06-12 | Charles T. Goodhue | Multilayer analytical elements for use in the assay of cholesterol |
| US3884764A (en) * | 1974-03-25 | 1975-05-20 | Eastman Kodak Co | Method and composition for blood serum cholesterol analysis |
| US4116773A (en) * | 1976-07-01 | 1978-09-26 | Beckman Instruments, Inc. | Enzymatic reagent and method for increasing the sensitivity of enzymatic substrate analyses using oxygen rate analyzers |
| DE2649749A1 (de) * | 1976-10-29 | 1978-05-11 | Boehringer Mannheim Gmbh | Verfahren und reagens zur bestimmung von gesamtcholesterin oder gebundenem cholesterin |
| DE2653537C3 (de) | 1976-11-25 | 1979-08-23 | Merck Patent Gmbh, 6100 Darmstadt | Verfahren und Mittel zur Bestimmung von Hydroperoxiden |
| ATE8912T1 (de) * | 1980-07-10 | 1984-08-15 | Ivan Endre Modrovich | Stabilisierte enzymatische loesungen und verfahren zum bestimmen von gesamtcholesterin im menschlichen serum. |
| US5047327A (en) * | 1982-04-02 | 1991-09-10 | Ivan E. Modrovich | Time-stable liquid cholesterol assay compositions |
| EP2502079B1 (en) | 2009-11-20 | 2015-01-28 | Pharnext | New diagnostic tools for charcot-marie-tooth disease |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR1422881A (fr) * | 1963-08-16 | 1966-01-03 | Warner Lambert Pharmaceutical | Composition auxiliaire de diagnostic utilisable pour déterminer le cholestérol |
| NL137003C (enrdf_load_stackoverflow) * | 1967-10-14 | |||
| FR2047147A5 (enrdf_load_stackoverflow) * | 1969-05-06 | 1971-03-12 | Kyowa Hakko Kogyo Kk | |
| DE2130340C3 (de) * | 1971-06-18 | 1975-07-03 | Boehringer Mannheim Gmbh, 6800 Mannheim | Verfahren und Vorrichtung zur quantitativen Bestimmung von einzelnen Proben unterschiedlicher Konzentration von Substanzen |
| GB1385319A (en) * | 1971-09-22 | 1975-02-26 | Nat Res Dev | Enzyme preparations |
-
1972
- 1972-05-17 DE DE19722224132 patent/DE2224132C2/de not_active Expired
- 1972-05-17 DE DE2264847A patent/DE2264847C2/de not_active Expired
-
1973
- 1973-05-14 CH CH681273A patent/CH589295A5/xx not_active IP Right Cessation
- 1973-05-15 AT AT426273A patent/AT324580B/de active
- 1973-05-15 BE BE131117A patent/BE799543A/xx not_active IP Right Cessation
- 1973-05-15 GB GB2308273A patent/GB1429525A/en not_active Expired
- 1973-05-15 NL NL7306738A patent/NL181282C/xx not_active IP Right Cessation
- 1973-05-17 FR FR7317898A patent/FR2191743A5/fr not_active Expired
Also Published As
| Publication number | Publication date |
|---|---|
| NL181282C (nl) | 1987-07-16 |
| AU5586173A (en) | 1974-11-21 |
| DE2264847C2 (de) | 1978-11-30 |
| NL7306738A (enrdf_load_stackoverflow) | 1973-11-20 |
| AT324580B (de) | 1975-09-10 |
| BE799543A (fr) | 1973-11-16 |
| CH589295A5 (enrdf_load_stackoverflow) | 1977-06-30 |
| FR2191743A5 (enrdf_load_stackoverflow) | 1974-02-01 |
| DE2224132A1 (enrdf_load_stackoverflow) | 1973-11-22 |
| DE2264847A1 (de) | 1975-05-28 |
| DE2224132B1 (de) | 1973-11-22 |
| NL181282B (nl) | 1987-02-16 |
| GB1429525A (en) | 1976-03-24 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| EP0354441B1 (de) | Verfahren zur kolorimetrischen Bestimmung eines Analyten mittels enzymatischer Oxidation | |
| EP0037056B1 (de) | Verfahren und diagnostische Mittel zum Nachweis von Redox-Reaktionen | |
| DE2913553C2 (de) | Verfahren und Reagenz zur enzymatischen Bestimmung von Enzymsubstraten | |
| DE2265122A1 (de) | Verfahren und mittel zur bestimmung von cholesterin | |
| DE2224132C2 (de) | Verfahren und Reagenz zur Bestimmung von Cholesterin | |
| US4212938A (en) | Reagent and method for the determination of cholesterol | |
| DE3872998T2 (de) | Test auf okkultes blut im stuhl. | |
| DE3003490A1 (de) | Reagenz zur analytischen bestimmung von wasserstoffperoxid | |
| DE2833612A1 (de) | Mittel und verfahren zur bestimmung von wasserstoffperoxyd | |
| DE3788828T2 (de) | Stabilisierte flüssige Enzymzusammensetzung zur Bestimmung von Glucose. | |
| EP0071730A1 (de) | Stabilisiertes Reagenz zum Nachweis von Wasserstoffperoxid | |
| DE2649749C2 (enrdf_load_stackoverflow) | ||
| DE2818603A1 (de) | Kinetisches glucosereagens und dessen verwendung in einem kinetischen nachweisverfahren | |
| DE2326756C3 (de) | Verfahren zur quantitativen Bestimmung von Harnsäure | |
| DE2906732C2 (de) | Verfahren und Reagenz zur Bestimmung der Peroxidase | |
| DE2834706A1 (de) | Verfahren und reagens zur bestimmung von glutamat-oxalacetat-transaminase und glutamat-pyruvat-transaminase | |
| DE2439348B2 (de) | Diagnostisches mittel zum nachweis und zur bestimmung von cholesterin und cholesterinestern in koerperfluessigkeiten | |
| DE2459087A1 (de) | Verfahren und reagenz zur bestimmung von serumtriglyceriden | |
| DE3443415A1 (de) | Zusammensetzung zur bestimmung von wasserstoffperoxid | |
| DE69128181T2 (de) | Enzymtests | |
| EP0228046A2 (de) | Verfahren zur Steigerung der Quanten-Ausbeute bei der Oxidation von Luminol durch Peroxide in Gegenwart von Peroxidase | |
| DE2612725C3 (de) | Verfahren und Reagenz zur Bestimmung von Cholesterin | |
| DE69709485T2 (de) | Reagenszusammensetzung, Teststück und Testkit | |
| DE2718588C3 (de) | Verfahren und Reagens zur Bestimmung von Harnsäure | |
| EP0011107B1 (de) | Methode zur Bestimmung einer Amino- oder Aminosulfonsäure |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| OI | Miscellaneous see part 1 | ||
| OI | Miscellaneous see part 1 | ||
| AG | Has addition no. |
Ref country code: DE Ref document number: 2265122 Format of ref document f/p: P |