DE2145936C2 - Verfahren zum Isolieren von Protein aus Fischen oder Fischprodukten - Google Patents
Verfahren zum Isolieren von Protein aus Fischen oder FischproduktenInfo
- Publication number
- DE2145936C2 DE2145936C2 DE2145936A DE2145936A DE2145936C2 DE 2145936 C2 DE2145936 C2 DE 2145936C2 DE 2145936 A DE2145936 A DE 2145936A DE 2145936 A DE2145936 A DE 2145936A DE 2145936 C2 DE2145936 C2 DE 2145936C2
- Authority
- DE
- Germany
- Prior art keywords
- protein
- fish
- solution
- solvent
- temperature
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 102000004169 proteins and genes Human genes 0.000 title claims description 51
- 108090000623 proteins and genes Proteins 0.000 title claims description 51
- 238000000034 method Methods 0.000 title claims description 27
- 241000251468 Actinopterygii Species 0.000 title claims description 18
- 235000013332 fish product Nutrition 0.000 title claims 7
- 239000000243 solution Substances 0.000 claims description 33
- 239000002904 solvent Substances 0.000 claims description 33
- 239000012460 protein solution Substances 0.000 claims description 20
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 15
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical group CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 claims description 14
- 239000003513 alkali Substances 0.000 claims description 14
- 108090000790 Enzymes Proteins 0.000 claims description 11
- 102000004190 Enzymes Human genes 0.000 claims description 11
- 229940088598 enzyme Drugs 0.000 claims description 11
- 239000000126 substance Substances 0.000 claims description 11
- 238000011282 treatment Methods 0.000 claims description 10
- 235000019733 Fish meal Nutrition 0.000 claims description 9
- 238000000605 extraction Methods 0.000 claims description 9
- 239000004467 fishmeal Substances 0.000 claims description 9
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 8
- 239000007788 liquid Substances 0.000 claims description 8
- NTIZESTWPVYFNL-UHFFFAOYSA-N Methyl isobutyl ketone Chemical compound CC(C)CC(C)=O NTIZESTWPVYFNL-UHFFFAOYSA-N 0.000 claims description 7
- UIHCLUNTQKBZGK-UHFFFAOYSA-N Methyl isobutyl ketone Natural products CCC(C)C(C)=O UIHCLUNTQKBZGK-UHFFFAOYSA-N 0.000 claims description 7
- 239000000047 product Substances 0.000 claims description 7
- 230000007062 hydrolysis Effects 0.000 claims description 6
- 238000006460 hydrolysis reaction Methods 0.000 claims description 6
- 239000002244 precipitate Substances 0.000 claims description 6
- 108090000526 Papain Proteins 0.000 claims description 5
- 239000004365 Protease Substances 0.000 claims description 5
- 229940055729 papain Drugs 0.000 claims description 5
- 235000019834 papain Nutrition 0.000 claims description 5
- 239000000843 powder Substances 0.000 claims description 5
- 239000011780 sodium chloride Substances 0.000 claims description 4
- 239000007858 starting material Substances 0.000 claims description 4
- 150000002632 lipids Chemical class 0.000 claims description 3
- 108091005804 Peptidases Proteins 0.000 claims description 2
- 102000035195 Peptidases Human genes 0.000 claims description 2
- 239000000470 constituent Substances 0.000 claims description 2
- 235000019688 fish Nutrition 0.000 claims 10
- 239000002609 medium Substances 0.000 claims 5
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 claims 2
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 claims 2
- 239000012736 aqueous medium Substances 0.000 claims 2
- 238000004519 manufacturing process Methods 0.000 claims 2
- 235000016709 nutrition Nutrition 0.000 claims 2
- BHPQYMZQTOCNFJ-UHFFFAOYSA-N Calcium cation Chemical compound [Ca+2] BHPQYMZQTOCNFJ-UHFFFAOYSA-N 0.000 claims 1
- 108010028690 Fish Proteins Proteins 0.000 claims 1
- AXCZMVOFGPJBDE-UHFFFAOYSA-L calcium dihydroxide Chemical compound [OH-].[OH-].[Ca+2] AXCZMVOFGPJBDE-UHFFFAOYSA-L 0.000 claims 1
- 239000000920 calcium hydroxide Substances 0.000 claims 1
- 229910001861 calcium hydroxide Inorganic materials 0.000 claims 1
- 229910001424 calcium ion Inorganic materials 0.000 claims 1
- 238000004090 dissolution Methods 0.000 claims 1
- 239000000463 material Substances 0.000 claims 1
- 238000006386 neutralization reaction Methods 0.000 claims 1
- 235000021032 oily fish Nutrition 0.000 claims 1
- 230000020477 pH reduction Effects 0.000 claims 1
- 238000002360 preparation method Methods 0.000 claims 1
- 230000002035 prolonged effect Effects 0.000 claims 1
- 239000002994 raw material Substances 0.000 claims 1
- 239000010802 sludge Substances 0.000 claims 1
- 238000003860 storage Methods 0.000 claims 1
- 235000018102 proteins Nutrition 0.000 description 26
- 238000005119 centrifugation Methods 0.000 description 13
- 239000007787 solid Substances 0.000 description 12
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 11
- 239000002002 slurry Substances 0.000 description 11
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 9
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 8
- 238000001816 cooling Methods 0.000 description 7
- RAHZWNYVWXNFOC-UHFFFAOYSA-N Sulphur dioxide Chemical compound O=S=O RAHZWNYVWXNFOC-UHFFFAOYSA-N 0.000 description 6
- VLKZOEOYAKHREP-UHFFFAOYSA-N n-Hexane Chemical compound CCCCCC VLKZOEOYAKHREP-UHFFFAOYSA-N 0.000 description 6
- 238000005406 washing Methods 0.000 description 6
- 238000010438 heat treatment Methods 0.000 description 5
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 4
- 238000001223 reverse osmosis Methods 0.000 description 4
- 102000004895 Lipoproteins Human genes 0.000 description 3
- 108090001030 Lipoproteins Proteins 0.000 description 3
- 239000000654 additive Substances 0.000 description 3
- 230000000996 additive effect Effects 0.000 description 3
- 235000013361 beverage Nutrition 0.000 description 3
- 210000000988 bone and bone Anatomy 0.000 description 3
- 239000003925 fat Substances 0.000 description 3
- 235000013305 food Nutrition 0.000 description 3
- 238000009987 spinning Methods 0.000 description 3
- 238000000108 ultra-filtration Methods 0.000 description 3
- 241001465754 Metazoa Species 0.000 description 2
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 2
- 238000011033 desalting Methods 0.000 description 2
- 238000001035 drying Methods 0.000 description 2
- 238000001125 extrusion Methods 0.000 description 2
- 238000005342 ion exchange Methods 0.000 description 2
- 239000012452 mother liquor Substances 0.000 description 2
- 239000013557 residual solvent Substances 0.000 description 2
- NWUYHJFMYQTDRP-UHFFFAOYSA-N 1,2-bis(ethenyl)benzene;1-ethenyl-2-ethylbenzene;styrene Chemical compound C=CC1=CC=CC=C1.CCC1=CC=CC=C1C=C.C=CC1=CC=CC=C1C=C NWUYHJFMYQTDRP-UHFFFAOYSA-N 0.000 description 1
- 235000019750 Crude protein Nutrition 0.000 description 1
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000005587 bubbling Effects 0.000 description 1
- 239000007795 chemical reaction product Substances 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000004042 decolorization Methods 0.000 description 1
- 239000000839 emulsion Substances 0.000 description 1
- 230000009931 harmful effect Effects 0.000 description 1
- 238000011221 initial treatment Methods 0.000 description 1
- 239000003456 ion exchange resin Substances 0.000 description 1
- 229920003303 ion-exchange polymer Polymers 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 239000002798 polar solvent Substances 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 235000004252 protein component Nutrition 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 230000007928 solubilization Effects 0.000 description 1
- 238000005063 solubilization Methods 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 239000004575 stone Substances 0.000 description 1
- 239000004552 water soluble powder Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
- A23J1/04—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from fish or other sea animals
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
- Y10S530/855—Proteins from animals other than mammals or birds
- Y10S530/857—Fish; fish eggs; shell fish; crustacea
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Biochemistry (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Peptides Or Proteins (AREA)
- Meat, Egg Or Seafood Products (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Seasonings (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB4531170 | 1970-09-23 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DE2145936A1 DE2145936A1 (de) | 1972-03-30 |
| DE2145936C2 true DE2145936C2 (de) | 1982-12-23 |
Family
ID=10436739
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE2145936A Expired DE2145936C2 (de) | 1970-09-23 | 1971-09-14 | Verfahren zum Isolieren von Protein aus Fischen oder Fischprodukten |
Country Status (18)
Families Citing this family (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB1383223A (en) * | 1972-05-22 | 1975-02-05 | Nestle Sa | Soluble protein |
| GB1348241A (en) * | 1972-05-22 | 1974-03-13 | Nestle Sa | Fish protein isolate |
| US3978234A (en) * | 1972-05-22 | 1976-08-31 | Societe D'assistance Technique Pour Produits Nestle S.A. | Protein composition |
| JPS5336033B2 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) * | 1972-12-11 | 1978-09-30 | ||
| US4060642A (en) * | 1974-12-16 | 1977-11-29 | Tokai Regional Fisheries Research Laboratory | Concentrated proteinaceous food material from marine animal meat |
| US4119619A (en) * | 1976-12-16 | 1978-10-10 | Sergei Vasilievich Rogozhin | Emulsification method for the processing of kriel to produce protein, lipids and chitin |
| SE421990B (sv) * | 1977-01-27 | 1982-02-15 | Alfa Laval Ab | Forfarande for uppdelning av animaliska ravaror i en fettfraktion och atminstone en proteinfraktion |
| DE2915734A1 (de) * | 1979-04-19 | 1980-11-06 | Hoechst Ag | Verfahren zur verbesserung der eigenschaften von rohproteinen tierischen ursprungs |
| NO147625C (no) * | 1980-05-14 | 1983-05-25 | Mustad & Soen As | Stimuliopploesning til bruk i fiskeagn eller fiskefor |
| US20020182290A1 (en) * | 2001-05-01 | 2002-12-05 | Novozymes A/S | Method for processing fish material |
| US20040038391A1 (en) * | 2002-02-06 | 2004-02-26 | Pyntikov Alexander V. | Amino acids factory |
| US6960451B2 (en) * | 2002-02-06 | 2005-11-01 | Green Earth Industries | Proteolytic fermenter |
| US20040203134A1 (en) * | 2002-02-06 | 2004-10-14 | Pyntikov Alexander V. | Complex technologies using enzymatic protein hydrolysate |
| FR2835703B1 (fr) * | 2002-02-08 | 2006-05-05 | Techniagro | Procede d'obtention d'une huile et d'un hydrolysat de proteines a partir d'une source marine de tissus proteiques et huile et hydrolysat de proteines obtenus par mise en oeuvre de ce procede |
| JP4226299B2 (ja) * | 2002-10-22 | 2009-02-18 | 日本水産株式会社 | 魚由来ゼラチンペプチドの製造方法 |
| DK175501B1 (da) * | 2002-12-02 | 2004-11-15 | Green Earth | Anlæg og fremgangsmåde til kontinuerlig hydrolyse af et proteinholdigt animalsk eller vegetabilsk råmateriale |
| EP1622466A4 (en) * | 2003-05-15 | 2007-02-14 | Rhode Island Education | BIOPRODUCTION OF HYDROLYSAT FROM CALAMAR SECONDARY PRODUCTS FOR THE MANUFACTURE OF AQUACULTURE FOOD INGREDIENTS AND ORGANIC FERTILIZERS |
| US20060099305A1 (en) * | 2004-05-17 | 2006-05-11 | Lee Chong M | Bioproduction of hydrolysate from squid processing byproducts for aquaculture feed ingredient and organic fertilizer |
| DE102011050905A1 (de) * | 2011-06-07 | 2012-12-13 | Gea Mechanical Equipment Gmbh | Verfahren zur Gewinnung von Proteinen aus einem nativen Stoffgemenge |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE837643C (de) * | 1948-06-22 | 1952-04-28 | Hugh Hanchard Goodwin | Verfahren zur Gewinnung von OEl und Proteinen aus Fisch |
-
1970
- 1970-09-23 GB GB4531170A patent/GB1322243A/en not_active Expired
-
1971
- 1971-08-30 ZA ZA715795A patent/ZA715795B/xx unknown
- 1971-08-31 CA CA121,820A patent/CA959335A/en not_active Expired
- 1971-09-01 YU YU02233/71A patent/YU36431B/xx unknown
- 1971-09-03 CH CH1293671A patent/CH528869A/fr not_active IP Right Cessation
- 1971-09-06 AU AU33112/71A patent/AU454090B2/en not_active Expired
- 1971-09-06 BE BE772237A patent/BE772237A/xx not_active IP Right Cessation
- 1971-09-10 SE SE7111501A patent/SE383824B/xx unknown
- 1971-09-10 US US00179586A patent/US3798126A/en not_active Expired - Lifetime
- 1971-09-13 NL NL7112557.A patent/NL157490B/xx not_active IP Right Cessation
- 1971-09-13 FI FI2556/71A patent/FI53776C/fi active
- 1971-09-14 DE DE2145936A patent/DE2145936C2/de not_active Expired
- 1971-09-15 NO NO3432/71A patent/NO132573C/no unknown
- 1971-09-17 IT IT28776/71A patent/IT972037B/it active
- 1971-09-22 JP JP7418571A patent/JPS552938B1/ja active Pending
- 1971-09-22 FR FR7134144A patent/FR2108431A5/fr not_active Expired
- 1971-09-22 PL PL1971150627A patent/PL84995B1/pl unknown
- 1971-09-22 ES ES395315A patent/ES395315A1/es not_active Expired
Also Published As
| Publication number | Publication date |
|---|---|
| AU3311271A (en) | 1973-03-15 |
| BE772237A (fr) | 1972-03-08 |
| DE2145936A1 (de) | 1972-03-30 |
| NL157490B (nl) | 1978-08-15 |
| IT972037B (it) | 1974-05-20 |
| ZA715795B (en) | 1972-04-26 |
| AU454090B2 (en) | 1974-10-17 |
| GB1322243A (en) | 1973-07-04 |
| NO132573B (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) | 1975-08-25 |
| NO132573C (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) | 1975-12-03 |
| JPS552938B1 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) | 1980-01-23 |
| FI53776B (fi) | 1978-05-02 |
| CH528869A (fr) | 1972-10-15 |
| FR2108431A5 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) | 1972-05-19 |
| YU223371A (en) | 1982-02-25 |
| NL7112557A (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) | 1972-03-27 |
| PL84995B1 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) | 1976-04-30 |
| US3798126A (en) | 1974-03-19 |
| SE383824B (sv) | 1976-04-05 |
| YU36431B (en) | 1984-02-29 |
| CA959335A (en) | 1974-12-17 |
| FI53776C (fi) | 1978-08-10 |
| ES395315A1 (es) | 1973-12-01 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| OD | Request for examination | ||
| D2 | Grant after examination | ||
| 8339 | Ceased/non-payment of the annual fee |