DE2130308C3 - Verfahren und Vorrichtung zum Bestimmen von enzymatisch umsetzbaren Probensubstanzen - Google Patents
Verfahren und Vorrichtung zum Bestimmen von enzymatisch umsetzbaren ProbensubstanzenInfo
- Publication number
- DE2130308C3 DE2130308C3 DE2130308A DE2130308A DE2130308C3 DE 2130308 C3 DE2130308 C3 DE 2130308C3 DE 2130308 A DE2130308 A DE 2130308A DE 2130308 A DE2130308 A DE 2130308A DE 2130308 C3 DE2130308 C3 DE 2130308C3
- Authority
- DE
- Germany
- Prior art keywords
- solution
- sample
- buffer
- enzyme
- buffer solution
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 238000000034 method Methods 0.000 title claims description 30
- 239000000126 substance Substances 0.000 title claims description 24
- 102000004190 Enzymes Human genes 0.000 claims description 37
- 108090000790 Enzymes Proteins 0.000 claims description 37
- 239000000243 solution Substances 0.000 claims description 37
- 239000000523 sample Substances 0.000 claims description 35
- 239000007853 buffer solution Substances 0.000 claims description 34
- 239000012488 sample solution Substances 0.000 claims description 27
- 238000005259 measurement Methods 0.000 claims description 26
- 239000000872 buffer Substances 0.000 claims description 22
- 230000008859 change Effects 0.000 claims description 13
- 239000007864 aqueous solution Substances 0.000 claims description 4
- 230000008034 disappearance Effects 0.000 claims description 2
- 238000001514 detection method Methods 0.000 claims 1
- 229940088598 enzyme Drugs 0.000 description 32
- 238000006243 chemical reaction Methods 0.000 description 19
- 229930027945 nicotinamide-adenine dinucleotide Natural products 0.000 description 17
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 15
- XJLXINKUBYWONI-DQQFMEOOSA-N [[(2r,3r,4r,5r)-5-(6-aminopurin-9-yl)-3-hydroxy-4-phosphonooxyoxolan-2-yl]methoxy-hydroxyphosphoryl] [(2s,3r,4s,5s)-5-(3-carbamoylpyridin-1-ium-1-yl)-3,4-dihydroxyoxolan-2-yl]methyl phosphate Chemical compound NC(=O)C1=CC=C[N+]([C@@H]2[C@H]([C@@H](O)[C@H](COP([O-])(=O)OP(O)(=O)OC[C@@H]3[C@H]([C@@H](OP(O)(O)=O)[C@@H](O3)N3C4=NC=NC(N)=C4N=C3)O)O2)O)=C1 XJLXINKUBYWONI-DQQFMEOOSA-N 0.000 description 15
- 239000008103 glucose Substances 0.000 description 12
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 9
- 239000001301 oxygen Substances 0.000 description 9
- 229910052760 oxygen Inorganic materials 0.000 description 9
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- 108700040460 Hexokinases Proteins 0.000 description 8
- 210000002966 serum Anatomy 0.000 description 8
- 238000011088 calibration curve Methods 0.000 description 7
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- 229910019142 PO4 Inorganic materials 0.000 description 6
- 239000000463 material Substances 0.000 description 6
- BOPGDPNILDQYTO-NNYOXOHSSA-N nicotinamide-adenine dinucleotide Chemical compound C1=CCC(C(=O)N)=CN1[C@H]1[C@H](O)[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OC[C@@H]2[C@H]([C@@H](O)[C@@H](O2)N2C3=NC=NC(N)=C3N=C2)O)O1 BOPGDPNILDQYTO-NNYOXOHSSA-N 0.000 description 6
- 239000010452 phosphate Substances 0.000 description 6
- 230000008569 process Effects 0.000 description 6
- LEHOTFFKMJEONL-UHFFFAOYSA-N Uric Acid Chemical compound N1C(=O)NC(=O)C2=C1NC(=O)N2 LEHOTFFKMJEONL-UHFFFAOYSA-N 0.000 description 5
- TVWHNULVHGKJHS-UHFFFAOYSA-N Uric acid Natural products N1C(=O)NC(=O)C2NC(=O)NC21 TVWHNULVHGKJHS-UHFFFAOYSA-N 0.000 description 5
- 229940116269 uric acid Drugs 0.000 description 5
- 101710088194 Dehydrogenase Proteins 0.000 description 4
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 4
- 229940024606 amino acid Drugs 0.000 description 4
- 230000015572 biosynthetic process Effects 0.000 description 4
- 239000003153 chemical reaction reagent Substances 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- BAWFJGJZGIEFAR-NNYOXOHSSA-N NAD zwitterion Chemical compound NC(=O)C1=CC=C[N+]([C@H]2[C@@H]([C@H](O)[C@@H](COP([O-])(=O)OP(O)(=O)OC[C@@H]3[C@H]([C@@H](O)[C@@H](O3)N3C4=NC=NC(N)=C4N=C3)O)O2)O)=C1 BAWFJGJZGIEFAR-NNYOXOHSSA-N 0.000 description 3
- LCTONWCANYUPML-UHFFFAOYSA-M Pyruvate Chemical compound CC(=O)C([O-])=O LCTONWCANYUPML-UHFFFAOYSA-M 0.000 description 3
- 108010092464 Urate Oxidase Proteins 0.000 description 3
- 150000001413 amino acids Chemical class 0.000 description 3
- 238000004458 analytical method Methods 0.000 description 3
- 238000010276 construction Methods 0.000 description 3
- 238000005553 drilling Methods 0.000 description 3
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- 229950006238 nadide Drugs 0.000 description 3
- 230000003287 optical effect Effects 0.000 description 3
- KHPXUQMNIQBQEV-UHFFFAOYSA-N oxaloacetic acid Chemical compound OC(=O)CC(=O)C(O)=O KHPXUQMNIQBQEV-UHFFFAOYSA-N 0.000 description 3
- 229920001296 polysiloxane Polymers 0.000 description 3
- NLKNQRATVPKPDG-UHFFFAOYSA-M potassium iodide Chemical compound [K+].[I-] NLKNQRATVPKPDG-UHFFFAOYSA-M 0.000 description 3
- 230000035945 sensitivity Effects 0.000 description 3
- BIRSGZKFKXLSJQ-SQOUGZDYSA-N 6-Phospho-D-gluconate Chemical compound OP(=O)(O)OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C(O)=O BIRSGZKFKXLSJQ-SQOUGZDYSA-N 0.000 description 2
- LRFVTYWOQMYALW-UHFFFAOYSA-N 9H-xanthine Chemical compound O=C1NC(=O)NC2=C1NC=N2 LRFVTYWOQMYALW-UHFFFAOYSA-N 0.000 description 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 102000016938 Catalase Human genes 0.000 description 2
- 108010053835 Catalase Proteins 0.000 description 2
- NBSCHQHZLSJFNQ-GASJEMHNSA-N D-Glucose 6-phosphate Chemical compound OC1O[C@H](COP(O)(O)=O)[C@@H](O)[C@H](O)[C@H]1O NBSCHQHZLSJFNQ-GASJEMHNSA-N 0.000 description 2
- VFRROHXSMXFLSN-UHFFFAOYSA-N Glc6P Natural products OP(=O)(O)OCC(O)C(O)C(O)C(O)C=O VFRROHXSMXFLSN-UHFFFAOYSA-N 0.000 description 2
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 2
- WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 description 2
- 108090000854 Oxidoreductases Proteins 0.000 description 2
- 102000004316 Oxidoreductases Human genes 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 2
- POJWUDADGALRAB-UHFFFAOYSA-N allantoin Chemical compound NC(=O)NC1NC(=O)NC1=O POJWUDADGALRAB-UHFFFAOYSA-N 0.000 description 2
- 238000003776 cleavage reaction Methods 0.000 description 2
- 229930195712 glutamate Natural products 0.000 description 2
- FDGQSTZJBFJUBT-UHFFFAOYSA-N hypoxanthine Chemical compound O=C1NC=NC2=C1NC=N2 FDGQSTZJBFJUBT-UHFFFAOYSA-N 0.000 description 2
- 150000002500 ions Chemical class 0.000 description 2
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 2
- 235000019341 magnesium sulphate Nutrition 0.000 description 2
- 229940049920 malate Drugs 0.000 description 2
- BJEPYKJPYRNKOW-UHFFFAOYSA-N malic acid Chemical compound OC(=O)C(O)CC(O)=O BJEPYKJPYRNKOW-UHFFFAOYSA-N 0.000 description 2
- 229910052751 metal Inorganic materials 0.000 description 2
- 239000002184 metal Substances 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 230000003647 oxidation Effects 0.000 description 2
- 238000007254 oxidation reaction Methods 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 2
- 239000004033 plastic Substances 0.000 description 2
- 230000008929 regeneration Effects 0.000 description 2
- 238000011069 regeneration method Methods 0.000 description 2
- 238000005070 sampling Methods 0.000 description 2
- 230000007017 scission Effects 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- KPGXRSRHYNQIFN-UHFFFAOYSA-L 2-oxoglutarate(2-) Chemical compound [O-]C(=O)CCC(=O)C([O-])=O KPGXRSRHYNQIFN-UHFFFAOYSA-L 0.000 description 1
- POJWUDADGALRAB-PVQJCKRUSA-N Allantoin Natural products NC(=O)N[C@@H]1NC(=O)NC1=O POJWUDADGALRAB-PVQJCKRUSA-N 0.000 description 1
- QGZKDVFQNNGYKY-UHFFFAOYSA-O Ammonium Chemical compound [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 description 1
- ATRRKUHOCOJYRX-UHFFFAOYSA-N Ammonium bicarbonate Chemical compound [NH4+].OC([O-])=O ATRRKUHOCOJYRX-UHFFFAOYSA-N 0.000 description 1
- BTBUEUYNUDRHOZ-UHFFFAOYSA-N Borate Chemical compound [O-]B([O-])[O-] BTBUEUYNUDRHOZ-UHFFFAOYSA-N 0.000 description 1
- 108050008072 Cytochrome c oxidase subunit IV Proteins 0.000 description 1
- 102000000634 Cytochrome c oxidase subunit IV Human genes 0.000 description 1
- 102000018832 Cytochromes Human genes 0.000 description 1
- 108010052832 Cytochromes Proteins 0.000 description 1
- 239000004366 Glucose oxidase Substances 0.000 description 1
- 108010015776 Glucose oxidase Proteins 0.000 description 1
- UGQMRVRMYYASKQ-UHFFFAOYSA-N Hypoxanthine nucleoside Natural products OC1C(O)C(CO)OC1N1C(NC=NC2=O)=C2N=C1 UGQMRVRMYYASKQ-UHFFFAOYSA-N 0.000 description 1
- LKDRXBCSQODPBY-AMVSKUEXSA-N L-(-)-Sorbose Chemical compound OCC1(O)OC[C@H](O)[C@@H](O)[C@@H]1O LKDRXBCSQODPBY-AMVSKUEXSA-N 0.000 description 1
- JVTAAEKCZFNVCJ-REOHCLBHSA-N L-lactic acid Chemical compound C[C@H](O)C(O)=O JVTAAEKCZFNVCJ-REOHCLBHSA-N 0.000 description 1
- JVTAAEKCZFNVCJ-UHFFFAOYSA-M Lactate Chemical compound CC(O)C([O-])=O JVTAAEKCZFNVCJ-UHFFFAOYSA-M 0.000 description 1
- 241000283986 Lepus Species 0.000 description 1
- 102000004882 Lipase Human genes 0.000 description 1
- 108090001060 Lipase Proteins 0.000 description 1
- 239000004367 Lipase Substances 0.000 description 1
- 108090000128 Lipoxygenases Proteins 0.000 description 1
- 102000003820 Lipoxygenases Human genes 0.000 description 1
- 102000013460 Malate Dehydrogenase Human genes 0.000 description 1
- 108010026217 Malate Dehydrogenase Proteins 0.000 description 1
- SPYYZVSDULLIHD-UHFFFAOYSA-N OC(=O)CCCC(O)=O.OC(=O)CC(=O)C(O)=O Chemical compound OC(=O)CCCC(O)=O.OC(=O)CC(=O)C(O)=O SPYYZVSDULLIHD-UHFFFAOYSA-N 0.000 description 1
- 240000000220 Panda oleosa Species 0.000 description 1
- 235000016496 Panda oleosa Nutrition 0.000 description 1
- 101710098398 Probable alanine aminotransferase, mitochondrial Proteins 0.000 description 1
- 102000009609 Pyrophosphatases Human genes 0.000 description 1
- 108010009413 Pyrophosphatases Proteins 0.000 description 1
- 241000220317 Rosa Species 0.000 description 1
- 229910000831 Steel Inorganic materials 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 description 1
- 239000004809 Teflon Substances 0.000 description 1
- 229920006362 Teflon® Polymers 0.000 description 1
- 244000269722 Thea sinensis Species 0.000 description 1
- GSEJCLTVZPLZKY-UHFFFAOYSA-N Triethanolamine Chemical compound OCCN(CCO)CCO GSEJCLTVZPLZKY-UHFFFAOYSA-N 0.000 description 1
- 108010046334 Urease Proteins 0.000 description 1
- IKHGUXGNUITLKF-XPULMUKRSA-N acetaldehyde Chemical compound [14CH]([14CH3])=O IKHGUXGNUITLKF-XPULMUKRSA-N 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 229960000458 allantoin Drugs 0.000 description 1
- WQZGKKKJIJFFOK-PHYPRBDBSA-N alpha-D-galactose Chemical compound OC[C@H]1O[C@H](O)[C@H](O)[C@@H](O)[C@H]1O WQZGKKKJIJFFOK-PHYPRBDBSA-N 0.000 description 1
- 239000001099 ammonium carbonate Substances 0.000 description 1
- 235000012501 ammonium carbonate Nutrition 0.000 description 1
- QGAVSDVURUSLQK-UHFFFAOYSA-N ammonium heptamolybdate Chemical compound N.N.N.N.N.N.O.O.O.O.O.O.O.O.O.O.O.O.O.O.O.O.O.O.O.O.O.O.O.O.[Mo].[Mo].[Mo].[Mo].[Mo].[Mo].[Mo] QGAVSDVURUSLQK-UHFFFAOYSA-N 0.000 description 1
- 150000001450 anions Chemical class 0.000 description 1
- 229940009098 aspartate Drugs 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 108010051210 beta-Fructofuranosidase Proteins 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 239000004202 carbamide Substances 0.000 description 1
- 239000012159 carrier gas Substances 0.000 description 1
- 230000003197 catalytic effect Effects 0.000 description 1
- 239000007795 chemical reaction product Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 239000005515 coenzyme Substances 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000005859 coupling reaction Methods 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 230000018044 dehydration Effects 0.000 description 1
- 238000006297 dehydration reaction Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 235000014113 dietary fatty acids Nutrition 0.000 description 1
- XPPKVPWEQAFLFU-UHFFFAOYSA-J diphosphate(4-) Chemical compound [O-]P([O-])(=O)OP([O-])([O-])=O XPPKVPWEQAFLFU-UHFFFAOYSA-J 0.000 description 1
- 235000011180 diphosphates Nutrition 0.000 description 1
- 229930195729 fatty acid Natural products 0.000 description 1
- 239000000194 fatty acid Substances 0.000 description 1
- 150000004665 fatty acids Chemical class 0.000 description 1
- 238000005429 filling process Methods 0.000 description 1
- 229930182830 galactose Natural products 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 239000003365 glass fiber Substances 0.000 description 1
- 229940116332 glucose oxidase Drugs 0.000 description 1
- 235000019420 glucose oxidase Nutrition 0.000 description 1
- 239000001573 invertase Substances 0.000 description 1
- 235000011073 invertase Nutrition 0.000 description 1
- 229940116871 l-lactate Drugs 0.000 description 1
- 235000019421 lipase Nutrition 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 229910052756 noble gas Inorganic materials 0.000 description 1
- 230000036284 oxygen consumption Effects 0.000 description 1
- 230000002572 peristaltic effect Effects 0.000 description 1
- 150000002978 peroxides Chemical class 0.000 description 1
- -1 polytetrafluoroethylene Polymers 0.000 description 1
- 229920001343 polytetrafluoroethylene Polymers 0.000 description 1
- 239000004810 polytetrafluoroethylene Substances 0.000 description 1
- 235000020777 polyunsaturated fatty acids Nutrition 0.000 description 1
- 239000008057 potassium phosphate buffer Substances 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 150000003222 pyridines Chemical class 0.000 description 1
- 230000035484 reaction time Effects 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 230000000087 stabilizing effect Effects 0.000 description 1
- 239000010959 steel Substances 0.000 description 1
- 238000005728 strengthening Methods 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 229940075420 xanthine Drugs 0.000 description 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/02—Burettes; Pipettes
- B01L3/0289—Apparatus for withdrawing or distributing predetermined quantities of fluid
- B01L3/0293—Apparatus for withdrawing or distributing predetermined quantities of fluid for liquids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/001—Enzyme electrodes
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T137/00—Fluid handling
- Y10T137/8593—Systems
- Y10T137/86493—Multi-way valve unit
- Y10T137/86718—Dividing into parallel flow paths with recombining
- Y10T137/86759—Reciprocating
- Y10T137/86767—Spool
- Y10T137/86775—With internal passage
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/11—Automated chemical analysis
- Y10T436/117497—Automated chemical analysis with a continuously flowing sample or carrier stream
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Genetics & Genomics (AREA)
- Clinical Laboratory Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
Priority Applications (13)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE2130308A DE2130308C3 (de) | 1971-06-18 | 1971-06-18 | Verfahren und Vorrichtung zum Bestimmen von enzymatisch umsetzbaren Probensubstanzen |
| US00261763A US3838011A (en) | 1971-06-18 | 1972-06-12 | Process and apparatus for the quantitative determination of an enzymatically reactive substance |
| FR7221258A FR2142416A5 (enExample) | 1971-06-18 | 1972-06-13 | |
| IT25820/72A IT972153B (it) | 1971-06-18 | 1972-06-16 | Dispositivo per la determinazione quantitativa di sostenz |
| GB2822972A GB1392878A (en) | 1971-06-18 | 1972-06-16 | Process for the quantitative determination of enzymatically reactable substances and of enzyme activities |
| SU1797434A SU542484A3 (ru) | 1971-06-18 | 1972-06-16 | Способ количественного определени веществ, способных к ферментативному окислению в водном растворе |
| SE7207963A SE403518B (sv) | 1971-06-18 | 1972-06-16 | Sett att kvantitativt bestemma en zymatiskt omsettbara substanser jemte medel for dess genomforande |
| JP6029972A JPS5717256B1 (enExample) | 1971-06-18 | 1972-06-16 | |
| CA144,994A CA988818A (en) | 1971-06-18 | 1972-06-16 | Process for the quantitative determination of an enzymatically reactive substance |
| CH904572A CH575601A5 (enExample) | 1971-06-18 | 1972-06-16 | |
| DK303172A DK147163C (da) | 1971-06-18 | 1972-06-16 | Fremgangsmaade til kvantitativ bestemmelse af enzymatisk reagerende stoffer og apparat til udfoerelse af fremgangsmaaden |
| NLAANVRAGE7208230,A NL176586C (nl) | 1971-06-18 | 1972-06-16 | Werkwijze voor de kwantitatieve bepaling van stoffen en inrichting voor het uitvoeren van deze werkwijze. |
| BE784986A BE784986A (fr) | 1971-06-18 | 1972-06-16 | Procede de determination quantitative de substances |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE2130308A DE2130308C3 (de) | 1971-06-18 | 1971-06-18 | Verfahren und Vorrichtung zum Bestimmen von enzymatisch umsetzbaren Probensubstanzen |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| DE2130308A1 DE2130308A1 (de) | 1972-12-21 |
| DE2130308B2 DE2130308B2 (de) | 1974-12-05 |
| DE2130308C3 true DE2130308C3 (de) | 1975-07-24 |
Family
ID=5811144
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE2130308A Expired DE2130308C3 (de) | 1971-06-18 | 1971-06-18 | Verfahren und Vorrichtung zum Bestimmen von enzymatisch umsetzbaren Probensubstanzen |
Country Status (13)
| Country | Link |
|---|---|
| US (1) | US3838011A (enExample) |
| JP (1) | JPS5717256B1 (enExample) |
| BE (1) | BE784986A (enExample) |
| CA (1) | CA988818A (enExample) |
| CH (1) | CH575601A5 (enExample) |
| DE (1) | DE2130308C3 (enExample) |
| DK (1) | DK147163C (enExample) |
| FR (1) | FR2142416A5 (enExample) |
| GB (1) | GB1392878A (enExample) |
| IT (1) | IT972153B (enExample) |
| NL (1) | NL176586C (enExample) |
| SE (1) | SE403518B (enExample) |
| SU (1) | SU542484A3 (enExample) |
Families Citing this family (30)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5631957B2 (enExample) * | 1973-10-09 | 1981-07-24 | ||
| US3926734A (en) * | 1973-12-21 | 1975-12-16 | Owens Illinois Inc | Urea analysis |
| US3930957A (en) * | 1974-07-11 | 1976-01-06 | Honeywell Inc. | Apparatus and method for biological analysis |
| US4018651A (en) * | 1975-03-20 | 1977-04-19 | Canto Elio D | Method for regenerating glucose oxidase reagent |
| SE397731B (sv) * | 1975-03-27 | 1977-11-14 | Servochem | Steriliserbar och regenererbar enzymelektrod samt sett att framstella densamma |
| DE2526558C2 (de) * | 1975-06-13 | 1984-11-29 | Boehringer Mannheim Gmbh, 6800 Mannheim | Verfahren zur enzymatischen Analyse |
| US3975238A (en) * | 1975-06-26 | 1976-08-17 | General Electric Company | Method and apparatus for detecting molecules in solutions |
| US4032406A (en) * | 1975-12-04 | 1977-06-28 | Monsanto Company | Dopamine-β-hydroxylase determination method |
| SE396819B (sv) * | 1975-12-31 | 1977-10-03 | Gambro Ab | Sett och anordning for bestemning av koncentrationen av en lagmolekyler forening i ett komplext medium genom dialys |
| DE2642232C3 (de) * | 1976-09-20 | 1980-05-14 | Boehringer Mannheim Gmbh, 6800 Mannheim | Verfahren und Vorrichtung zur laufenden Bestimmung der Konzentration eines Enzymsubstrates |
| NL7708564A (nl) * | 1977-08-03 | 1979-02-06 | Philips Nv | Inrichting voor enzymatische analyse. |
| US4347313A (en) * | 1978-02-27 | 1982-08-31 | Boehringer Mannheim Gmbh | Analytical determination of lipase |
| US4243753A (en) * | 1978-02-27 | 1981-01-06 | Purdue Research Foundation | Apparatus for enzyme detection |
| US4247647A (en) * | 1978-03-14 | 1981-01-27 | Technicon Instruments Corporation | Apparatus for the quantitative determination of saccharides |
| US4350763A (en) * | 1978-03-23 | 1982-09-21 | Ajinomoto Company, Inc. | Method for determining biochemical oxygen demand |
| US4242446A (en) * | 1978-07-26 | 1980-12-30 | Coulter Electronics, Inc. | Method for determining a substance in a biological fluid and reagent combination for use in the method |
| US4371611A (en) * | 1978-08-07 | 1983-02-01 | W. R. Grace & Co. | Enzymatic diagnostic composition |
| US4226935A (en) * | 1978-08-07 | 1980-10-07 | W. R. Grace & Co. | Enzymatic diagnostic composition |
| US4340448A (en) * | 1978-08-28 | 1982-07-20 | University Of Pittsburgh | Potentiometric detection of hydrogen peroxide and apparatus therefor |
| JPS5718979A (en) | 1980-07-09 | 1982-01-30 | Olympus Optical Co Ltd | Measuring container for use in analysis with immobilized enzyme |
| ATE4328T1 (de) * | 1980-09-19 | 1983-08-15 | Boehringer Mannheim Gmbh | Verfahren und reagens zur bestimmung von glycerin. |
| JPS58179866U (ja) * | 1982-05-24 | 1983-12-01 | 三菱重工業株式会社 | オルタネ−タ駆動系の緩衝装置 |
| US4655880A (en) * | 1983-08-01 | 1987-04-07 | Case Western Reserve University | Apparatus and method for sensing species, substances and substrates using oxidase |
| US4652520A (en) * | 1984-07-16 | 1987-03-24 | Immuno-Mycologics, Inc. | Comparative assay method and device |
| US5045284A (en) * | 1988-11-22 | 1991-09-03 | The Dow Chemical Company | Flow cell for precipitation titration flow injection analysis |
| US5205988A (en) * | 1989-04-06 | 1993-04-27 | Nihon Bunko Kogyo Kabushiki Kaisha | Apparatus for measuring gaseous aldehyde |
| CA2024548C (en) * | 1989-09-05 | 2002-05-28 | David Issachar | Analyte specific chemical sensor |
| US7641660B2 (en) | 2004-03-08 | 2010-01-05 | Biomet Manufacturing Corporation | Method, apparatus, and system for image guided bone cutting |
| DE102007017681A1 (de) * | 2007-04-14 | 2009-01-08 | Papst Licensing Gmbh & Co. Kg | Vorrichtung und Verfahren zum Messen der Aktivität von Enzymen nach Inhibitorentzug |
| CN114042524B (zh) * | 2021-11-29 | 2022-12-09 | 河南猎金科技开发有限公司 | 一种颚式破碎机投料辅助装置 |
-
1971
- 1971-06-18 DE DE2130308A patent/DE2130308C3/de not_active Expired
-
1972
- 1972-06-12 US US00261763A patent/US3838011A/en not_active Expired - Lifetime
- 1972-06-13 FR FR7221258A patent/FR2142416A5/fr not_active Expired
- 1972-06-16 BE BE784986A patent/BE784986A/xx not_active IP Right Cessation
- 1972-06-16 CH CH904572A patent/CH575601A5/xx not_active IP Right Cessation
- 1972-06-16 CA CA144,994A patent/CA988818A/en not_active Expired
- 1972-06-16 SE SE7207963A patent/SE403518B/xx unknown
- 1972-06-16 GB GB2822972A patent/GB1392878A/en not_active Expired
- 1972-06-16 JP JP6029972A patent/JPS5717256B1/ja active Pending
- 1972-06-16 DK DK303172A patent/DK147163C/da not_active IP Right Cessation
- 1972-06-16 SU SU1797434A patent/SU542484A3/ru active
- 1972-06-16 IT IT25820/72A patent/IT972153B/it active
- 1972-06-16 NL NLAANVRAGE7208230,A patent/NL176586C/xx not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| FR2142416A5 (enExample) | 1973-01-26 |
| CA988818A (en) | 1976-05-11 |
| GB1392878A (en) | 1975-05-07 |
| NL176586C (nl) | 1985-05-01 |
| BE784986A (fr) | 1972-12-18 |
| IT972153B (it) | 1974-05-20 |
| SU542484A3 (ru) | 1977-01-05 |
| NL7208230A (enExample) | 1972-12-20 |
| US3838011A (en) | 1974-09-24 |
| DK147163B (da) | 1984-04-30 |
| DE2130308A1 (de) | 1972-12-21 |
| SE403518B (sv) | 1978-08-21 |
| JPS5717256B1 (enExample) | 1982-04-09 |
| DK147163C (da) | 1984-10-15 |
| DE2130308B2 (de) | 1974-12-05 |
| CH575601A5 (enExample) | 1976-05-14 |
| NL176586B (nl) | 1984-12-03 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C3 | Grant after two publication steps (3rd publication) | ||
| E77 | Valid patent as to the heymanns-index 1977 | ||
| 8339 | Ceased/non-payment of the annual fee |