DE1598110A1 - Vorrichtung zur Elektrophoreses - Google Patents
Vorrichtung zur ElektrophoresesInfo
- Publication number
- DE1598110A1 DE1598110A1 DE19661598110 DE1598110A DE1598110A1 DE 1598110 A1 DE1598110 A1 DE 1598110A1 DE 19661598110 DE19661598110 DE 19661598110 DE 1598110 A DE1598110 A DE 1598110A DE 1598110 A1 DE1598110 A1 DE 1598110A1
- Authority
- DE
- Germany
- Prior art keywords
- buffer solution
- electrode
- flow
- chamber
- intermediate zone
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000001962 electrophoresis Methods 0.000 title claims description 14
- 239000007853 buffer solution Substances 0.000 claims description 63
- 230000004888 barrier function Effects 0.000 claims description 36
- 239000003792 electrolyte Substances 0.000 claims description 30
- 150000002500 ions Chemical class 0.000 claims description 26
- 238000005192 partition Methods 0.000 claims description 12
- 230000005012 migration Effects 0.000 claims description 9
- 238000013508 migration Methods 0.000 claims description 9
- 239000000126 substance Substances 0.000 claims description 7
- 238000012360 testing method Methods 0.000 claims description 5
- 239000000243 solution Substances 0.000 claims description 3
- 239000000872 buffer Substances 0.000 claims description 2
- 239000002245 particle Substances 0.000 description 19
- 239000007788 liquid Substances 0.000 description 7
- 230000005684 electric field Effects 0.000 description 6
- 239000000203 mixture Substances 0.000 description 5
- 239000012528 membrane Substances 0.000 description 4
- 230000009471 action Effects 0.000 description 3
- 230000008901 benefit Effects 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 230000010287 polarization Effects 0.000 description 3
- 238000000926 separation method Methods 0.000 description 3
- 229920005372 Plexiglas® Polymers 0.000 description 2
- 238000009825 accumulation Methods 0.000 description 2
- 238000004891 communication Methods 0.000 description 2
- 239000012777 electrically insulating material Substances 0.000 description 2
- 238000005868 electrolysis reaction Methods 0.000 description 2
- 239000012530 fluid Substances 0.000 description 2
- 238000011010 flushing procedure Methods 0.000 description 2
- 239000011521 glass Substances 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 239000004925 Acrylic resin Substances 0.000 description 1
- 229920000178 Acrylic resin Polymers 0.000 description 1
- VVQNEPGJFQJSBK-UHFFFAOYSA-N Methyl methacrylate Chemical compound COC(=O)C(C)=C VVQNEPGJFQJSBK-UHFFFAOYSA-N 0.000 description 1
- PMZURENOXWZQFD-UHFFFAOYSA-L Sodium Sulfate Chemical compound [Na+].[Na+].[O-]S([O-])(=O)=O PMZURENOXWZQFD-UHFFFAOYSA-L 0.000 description 1
- 150000001450 anions Chemical class 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000012928 buffer substance Substances 0.000 description 1
- 150000001768 cations Chemical class 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000000502 dialysis Methods 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 238000006073 displacement reaction Methods 0.000 description 1
- 239000008151 electrolyte solution Substances 0.000 description 1
- 238000005370 electroosmosis Methods 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 239000003014 ion exchange membrane Substances 0.000 description 1
- 210000003041 ligament Anatomy 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 230000003472 neutralizing effect Effects 0.000 description 1
- 125000002467 phosphate group Chemical class [H]OP(=O)(O[H])O[*] 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 239000004926 polymethyl methacrylate Substances 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000005086 pumping Methods 0.000 description 1
- 239000004627 regenerated cellulose Substances 0.000 description 1
- 229910052938 sodium sulfate Inorganic materials 0.000 description 1
- 235000011152 sodium sulphate Nutrition 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44756—Apparatus specially adapted therefor
- G01N27/44769—Continuous electrophoresis, i.e. the sample being continuously introduced, e.g. free flow electrophoresis [FFE]
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Molecular Biology (AREA)
- Chemical & Material Sciences (AREA)
- Physics & Mathematics (AREA)
- Electrochemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Electrostatic Separation (AREA)
- Separation Using Semi-Permeable Membranes (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US481679A US3412008A (en) | 1965-08-23 | 1965-08-23 | Electrophoresis apparatus |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DE1598110A1 true DE1598110A1 (de) | 1972-04-20 |
Family
ID=23912935
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE19661598110 Pending DE1598110A1 (de) | 1965-08-23 | 1966-05-21 | Vorrichtung zur Elektrophoreses |
Country Status (4)
| Country | Link |
|---|---|
| US (1) | US3412008A (cg-RX-API-DMAC7.html) |
| DE (1) | DE1598110A1 (cg-RX-API-DMAC7.html) |
| GB (1) | GB1088308A (cg-RX-API-DMAC7.html) |
| SE (1) | SE334489B (cg-RX-API-DMAC7.html) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0497077A1 (de) * | 1991-01-28 | 1992-08-05 | Ciba-Geigy Ag | Vorrichtung zur Vorbereitung von Proben insbesondere für Analysezwecke |
Families Citing this family (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3458428A (en) * | 1966-11-03 | 1969-07-29 | Beckman Instruments Inc | Continuous particle electrophoresis apparatus having improved particle band stability |
| US3509035A (en) * | 1967-04-14 | 1970-04-28 | Beckman Instruments Inc | Continuous particle electrophoresis cell |
| US3755132A (en) * | 1971-11-08 | 1973-08-28 | Univ California | Fluid belt electrophoresis apparatus |
| DE2508844C2 (de) * | 1975-02-28 | 1984-06-14 | Max Planck Gesellschaft zur Förderung der Wissenschaften e.V., 3400 Göttingen | Einrichtung zur trägerfreien kontinuierlichen Ablenkungselektrophorese |
| US4310408A (en) * | 1980-02-20 | 1982-01-12 | Mcdonnell Douglas Corporation | Electrophoresis chamber |
| US6767443B2 (en) | 1999-03-29 | 2004-07-27 | Percy H. Rhodes | Method and apparatus for electrophoretic focusing |
| US6171466B1 (en) | 1999-03-29 | 2001-01-09 | Percy H. Rhodes | Method and apparatus for electrophoretic focusing |
| US6660146B2 (en) | 1999-03-29 | 2003-12-09 | Percy H. Rhodes | Method for electrophoretic focusing |
| DE10047088C2 (de) * | 2000-09-21 | 2002-10-17 | Gerhard Weber | Medium für analytische und präparative Elektrophorese |
| DE10063097B4 (de) * | 2000-12-18 | 2007-04-19 | Becton, Dickinson And Co. | Elektrophoresevorrichtung |
| DE10063096C1 (de) * | 2000-12-18 | 2002-09-12 | Gerhard Weber | Elektrophoresevorrichtung, Elektrophoreseverfahren unter Verwendung einer Elektrophoresevorrichtung und Verwendung der Elektrophoresevorrichtung |
| DE102005020134A1 (de) | 2005-04-29 | 2006-11-02 | Becton, Dickinson And Co. | Verfahren und Vorrichtung zur Durchführung eines parallelen und simultanen Mehrfachprozesses der trägerfreien isoelektrischen Fokussierung |
| AU2007291260B2 (en) * | 2006-08-29 | 2013-09-05 | Becton, Dickinson And Company | Method and apparatus for carrier-free deflection electrophoresis |
| US9766206B2 (en) | 2013-09-27 | 2017-09-19 | ProteinSimple | Apparatus, systems, and methods for capillary electrophoresis |
| CN113348362A (zh) | 2019-01-25 | 2021-09-03 | 普诺森公司 | 用于毛细管电泳的可重复使用的盒 |
| EP4041427A4 (en) * | 2019-10-09 | 2023-11-08 | ProteinSimple | METHOD FOR SINGLE-CHANNEL FREE-FLOW ELECTROPHORESIS WITH SEQUENTIAL PH ADJUSTMENT |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US2555487A (en) * | 1948-02-27 | 1951-06-05 | United Shoe Machinery Corp | Chromatographic process and apparatus |
| US2879217A (en) * | 1956-06-07 | 1959-03-24 | Beckman Instruments Inc | Continuous electrophoresis apparatus with laminated wick electrodes |
-
1965
- 1965-08-23 US US481679A patent/US3412008A/en not_active Expired - Lifetime
-
1966
- 1966-05-05 GB GB19840/66A patent/GB1088308A/en not_active Expired
- 1966-05-20 SE SE06983/66A patent/SE334489B/xx unknown
- 1966-05-21 DE DE19661598110 patent/DE1598110A1/de active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0497077A1 (de) * | 1991-01-28 | 1992-08-05 | Ciba-Geigy Ag | Vorrichtung zur Vorbereitung von Proben insbesondere für Analysezwecke |
Also Published As
| Publication number | Publication date |
|---|---|
| SE334489B (cg-RX-API-DMAC7.html) | 1971-04-26 |
| US3412008A (en) | 1968-11-19 |
| GB1088308A (en) | 1967-10-25 |
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