DE1517775B2 - Verfahren zur herstellung eines milchkoagulierenden enzyms - Google Patents
Verfahren zur herstellung eines milchkoagulierenden enzymsInfo
- Publication number
- DE1517775B2 DE1517775B2 DE19661517775 DE1517775A DE1517775B2 DE 1517775 B2 DE1517775 B2 DE 1517775B2 DE 19661517775 DE19661517775 DE 19661517775 DE 1517775 A DE1517775 A DE 1517775A DE 1517775 B2 DE1517775 B2 DE 1517775B2
- Authority
- DE
- Germany
- Prior art keywords
- mucor
- strain
- milk
- culture
- enzyme
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Ceased
Links
- 108090000790 Enzymes Proteins 0.000 title claims description 22
- 102000004190 Enzymes Human genes 0.000 title claims description 22
- 238000004519 manufacturing process Methods 0.000 title claims description 9
- 229940088598 enzyme Drugs 0.000 claims description 21
- 238000000034 method Methods 0.000 claims description 16
- 241000235403 Rhizomucor miehei Species 0.000 claims description 14
- 235000013336 milk Nutrition 0.000 claims description 11
- 239000008267 milk Substances 0.000 claims description 11
- 210000004080 milk Anatomy 0.000 claims description 11
- 230000000694 effects Effects 0.000 claims description 10
- 230000001112 coagulating effect Effects 0.000 claims description 9
- 241000235395 Mucor Species 0.000 claims description 7
- 241000235525 Rhizomucor pusillus Species 0.000 claims description 7
- 235000015097 nutrients Nutrition 0.000 claims description 7
- 108010058314 rennet Proteins 0.000 claims description 7
- 229940108461 rennet Drugs 0.000 claims description 6
- 238000007654 immersion Methods 0.000 claims description 4
- 238000009395 breeding Methods 0.000 claims description 3
- 230000001488 breeding effect Effects 0.000 claims description 3
- 230000000052 comparative effect Effects 0.000 claims description 3
- 241000233866 Fungi Species 0.000 claims description 2
- 238000012136 culture method Methods 0.000 claims description 2
- 235000013351 cheese Nutrition 0.000 claims 6
- 230000002797 proteolythic effect Effects 0.000 claims 5
- 244000309466 calf Species 0.000 claims 3
- 239000005018 casein Substances 0.000 claims 3
- BECPQYXYKAMYBN-UHFFFAOYSA-N casein, tech. Chemical compound NCCCCC(C(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(CC(C)C)N=C(O)C(CCC(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(C(C)O)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(COP(O)(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(N)CC1=CC=CC=C1 BECPQYXYKAMYBN-UHFFFAOYSA-N 0.000 claims 3
- 235000021240 caseins Nutrition 0.000 claims 3
- 230000005070 ripening Effects 0.000 claims 3
- 210000002784 stomach Anatomy 0.000 claims 3
- 108091005804 Peptidases Proteins 0.000 claims 2
- 102000035195 Peptidases Human genes 0.000 claims 2
- 239000002609 medium Substances 0.000 claims 2
- 244000005700 microbiome Species 0.000 claims 2
- 235000019833 protease Nutrition 0.000 claims 2
- 241000228251 Aspergillus phoenicis Species 0.000 claims 1
- 244000063299 Bacillus subtilis Species 0.000 claims 1
- 235000014469 Bacillus subtilis Nutrition 0.000 claims 1
- 241001465754 Metazoa Species 0.000 claims 1
- 239000005862 Whey Substances 0.000 claims 1
- 102000007544 Whey Proteins Human genes 0.000 claims 1
- 108010046377 Whey Proteins Proteins 0.000 claims 1
- 238000007598 dipping method Methods 0.000 claims 1
- 230000002349 favourable effect Effects 0.000 claims 1
- 239000001963 growth medium Substances 0.000 claims 1
- 230000006698 induction Effects 0.000 claims 1
- 230000000813 microbial effect Effects 0.000 claims 1
- 230000000877 morphologic effect Effects 0.000 claims 1
- 102000004196 processed proteins & peptides Human genes 0.000 claims 1
- 108090000765 processed proteins & peptides Proteins 0.000 claims 1
- 239000000203 mixture Substances 0.000 description 9
- 235000013312 flour Nutrition 0.000 description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 7
- 235000010469 Glycine max Nutrition 0.000 description 5
- 238000002474 experimental method Methods 0.000 description 5
- 229920001817 Agar Polymers 0.000 description 4
- 240000005979 Hordeum vulgare Species 0.000 description 4
- 235000007340 Hordeum vulgare Nutrition 0.000 description 4
- 239000008272 agar Substances 0.000 description 4
- 238000000855 fermentation Methods 0.000 description 4
- 230000004151 fermentation Effects 0.000 description 4
- 229920001592 potato starch Polymers 0.000 description 4
- 239000004382 Amylase Substances 0.000 description 3
- 102000013142 Amylases Human genes 0.000 description 3
- 108010065511 Amylases Proteins 0.000 description 3
- 235000019418 amylase Nutrition 0.000 description 3
- 230000001580 bacterial effect Effects 0.000 description 3
- 238000001816 cooling Methods 0.000 description 3
- 238000011534 incubation Methods 0.000 description 3
- 238000011081 inoculation Methods 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 239000000758 substrate Substances 0.000 description 3
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
- 229920002472 Starch Polymers 0.000 description 2
- 240000008042 Zea mays Species 0.000 description 2
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 2
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 2
- 238000005273 aeration Methods 0.000 description 2
- 229940041514 candida albicans extract Drugs 0.000 description 2
- 235000005822 corn Nutrition 0.000 description 2
- 239000008103 glucose Substances 0.000 description 2
- 235000012424 soybean oil Nutrition 0.000 description 2
- 239000003549 soybean oil Substances 0.000 description 2
- 239000008107 starch Substances 0.000 description 2
- 235000019698 starch Nutrition 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- 235000015099 wheat brans Nutrition 0.000 description 2
- 239000012138 yeast extract Substances 0.000 description 2
- 241000221756 Cryphonectria parasitica Species 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 241000219730 Lathyrus aphaca Species 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 239000002518 antifoaming agent Substances 0.000 description 1
- 239000006286 aqueous extract Substances 0.000 description 1
- 239000007900 aqueous suspension Substances 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 239000000706 filtrate Substances 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 230000002538 fungal effect Effects 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 235000012054 meals Nutrition 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 235000020183 skimmed milk Nutrition 0.000 description 1
- 230000028070 sporulation Effects 0.000 description 1
- 239000008223 sterile water Substances 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING OR TREATMENT THEREOF
- A23C19/00—Cheese; Cheese preparations; Making thereof
- A23C19/02—Making cheese curd
- A23C19/032—Making cheese curd characterised by the use of specific microorganisms, or enzymes of microbial origin
- A23C19/0326—Rennet produced by fermentation, e.g. microbial rennet; Rennet produced by genetic engineering
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/8215—Microorganisms
- Y10S435/911—Microorganisms using fungi
- Y10S435/931—Mucor
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Wood Science & Technology (AREA)
- Mycology (AREA)
- Biomedical Technology (AREA)
- Medicinal Chemistry (AREA)
- Polymers & Plastics (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Food Science & Technology (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB51270/65A GB1108287A (en) | 1965-12-02 | 1965-12-02 | Improvements in or relating to the preparation of a milk-coagulating enzyme |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DE1517775A1 DE1517775A1 (de) | 1970-01-29 |
| DE1517775B2 true DE1517775B2 (de) | 1973-02-08 |
Family
ID=10459340
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE19661517775 Ceased DE1517775B2 (de) | 1965-12-02 | 1966-11-30 | Verfahren zur herstellung eines milchkoagulierenden enzyms |
Country Status (14)
Families Citing this family (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4136201A (en) * | 1967-12-06 | 1979-01-23 | Gb Fermentation Industries, Inc. | Microbial rennin |
| US3549390A (en) * | 1968-09-09 | 1970-12-22 | Miles Lab | Milk-clotting enzyme product and process therefor |
| US4116219A (en) * | 1976-04-22 | 1978-09-26 | Robertshaw Controls Company | Differential thermostatic controller for solar heating system |
| USRE30669E (en) * | 1979-10-15 | 1981-07-07 | Gist-Brocades N.V. | Microbial rennin |
| WO2003052092A2 (en) * | 2001-12-19 | 2003-06-26 | Dsm Ip Assets B.V. | Method for the inactivation of amylase in the presence of protease |
| EP1507858B1 (en) * | 2002-05-29 | 2008-09-17 | DSM IP Assets B.V. | Method for the purification of a rhizomucor aspartic protease |
| EP2333056B1 (en) | 2006-04-13 | 2014-05-07 | DSM IP Assets B.V. | Liquid composition comprising an aspartic protease |
| US8703463B2 (en) | 2009-03-10 | 2014-04-22 | Dsm Ip Assets B.V. | Pregastric esterase and derivatives thereof |
| EP2516640A2 (en) | 2009-12-22 | 2012-10-31 | Novozymes A/S | Pullulanase variants and uses thereof |
| CA2795806C (en) | 2010-04-14 | 2019-01-08 | Novozymes A/S | Polypeptides having glucoamylase activity and polynucleotides encoding same |
| US20190161786A1 (en) | 2016-04-07 | 2019-05-30 | Novozymes A/S | Methods for selecting enzymes having protease activity |
| PT3638044T (pt) | 2017-06-15 | 2024-06-12 | Dsm Ip Assets Bv | Péletes de enzima congeladas |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3212905A (en) * | 1961-07-20 | 1965-10-19 | Meito Sangyo Kk | Process for making cheese |
-
0
- DK DK110031D patent/DK110031A/da unknown
-
1965
- 1965-12-02 GB GB51270/65A patent/GB1108287A/en not_active Expired
-
1966
- 1966-11-21 US US04/595,643 patent/US3988207A/en not_active Expired - Lifetime
- 1966-11-22 CA CA000976177A patent/CA1137898A/en not_active Expired
- 1966-11-24 SE SE6616104A patent/SE347288C/xx unknown
- 1966-11-25 FI FI3123/66A patent/FI42693B/fi active
- 1966-11-29 NO NO165794A patent/NO120183B/no unknown
- 1966-11-29 ES ES0333922A patent/ES333922A1/es not_active Expired
- 1966-11-30 CH CH1721066A patent/CH470477A/de not_active IP Right Cessation
- 1966-11-30 DE DE19661517775 patent/DE1517775B2/de not_active Ceased
- 1966-11-30 BR BR185010/66A patent/BR6685010D0/pt unknown
- 1966-12-01 BE BE690571D patent/BE690571A/xx not_active IP Right Cessation
- 1966-12-01 AT AT1114066A patent/AT282511B/de not_active IP Right Cessation
- 1966-12-02 NL NL666617035A patent/NL147782B/xx not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| CH470477A (de) | 1969-03-31 |
| AT282511B (de) | 1970-06-25 |
| BE690571A (enrdf_load_stackoverflow) | 1967-06-01 |
| ES333922A1 (es) | 1967-11-01 |
| DE1517775A1 (de) | 1970-01-29 |
| NL6617035A (enrdf_load_stackoverflow) | 1967-06-05 |
| GB1108287A (en) | 1968-04-03 |
| CA1137898A (en) | 1982-12-21 |
| FI42693B (enrdf_load_stackoverflow) | 1970-06-30 |
| DK110031A (enrdf_load_stackoverflow) | |
| NO120183B (enrdf_load_stackoverflow) | 1970-09-14 |
| BR6685010D0 (pt) | 1973-10-25 |
| SE347288B (enrdf_load_stackoverflow) | 1972-07-31 |
| US3988207A (en) | 1976-10-26 |
| SE347288C (sv) | 1977-11-07 |
| NL147782B (nl) | 1975-11-17 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 8235 | Patent refused |