DE112023001290T5 - Modifizierte Proteinglutaminase - Google Patents

Modifizierte Proteinglutaminase Download PDF

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Publication number
DE112023001290T5
DE112023001290T5 DE112023001290.3T DE112023001290T DE112023001290T5 DE 112023001290 T5 DE112023001290 T5 DE 112023001290T5 DE 112023001290 T DE112023001290 T DE 112023001290T DE 112023001290 T5 DE112023001290 T5 DE 112023001290T5
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DE
Germany
Prior art keywords
residue
amino acid
substitution
mutant
acid residue
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
DE112023001290.3T
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German (de)
English (en)
Inventor
Tetsuya Takahashi
Atsushi Ono
Kazunori Yoshida
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Amano Enzyme Inc
Original Assignee
Amano Enzyme Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Amano Enzyme Inc filed Critical Amano Enzyme Inc
Publication of DE112023001290T5 publication Critical patent/DE112023001290T5/de
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C12N9/80—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in linear amides (3.5.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y305/00—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5)
    • C12Y305/01—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5) in linear amides (3.5.1)
    • C12Y305/01002—Glutaminase (3.5.1.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y305/00—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5)
    • C12Y305/01—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5) in linear amides (3.5.1)
    • C12Y305/01044—Protein-glutamine glutaminase (3.5.1.44)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
DE112023001290.3T 2022-03-09 2023-03-09 Modifizierte Proteinglutaminase Pending DE112023001290T5 (de)

Applications Claiming Priority (13)

Application Number Priority Date Filing Date Title
JP2022036683 2022-03-09
JP2022-036683 2022-03-09
JP2022072469 2022-04-26
JP2022-072469 2022-04-26
JP2022081567 2022-05-18
JP2022-081567 2022-05-18
JP2022-087170 2022-05-27
JP2022087170 2022-05-27
JP2022-100227 2022-06-22
JP2022100227 2022-06-22
JP2022122327 2022-07-29
JP2022-122327 2022-07-29
PCT/JP2023/009183 WO2023171778A1 (ja) 2022-03-09 2023-03-09 改変型プロテイングルタミナーゼ

Publications (1)

Publication Number Publication Date
DE112023001290T5 true DE112023001290T5 (de) 2025-01-02

Family

ID=87935307

Family Applications (1)

Application Number Title Priority Date Filing Date
DE112023001290.3T Pending DE112023001290T5 (de) 2022-03-09 2023-03-09 Modifizierte Proteinglutaminase

Country Status (5)

Country Link
US (1) US20250188435A1 (https=)
EP (1) EP4491731A1 (https=)
JP (1) JPWO2023171778A1 (https=)
DE (1) DE112023001290T5 (https=)
WO (1) WO2023171778A1 (https=)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2025164794A1 (ja) * 2024-02-01 2025-08-07 天野エンザイム株式会社 改変型プロテイングルタミナーゼ
WO2025239456A1 (ja) * 2024-05-17 2025-11-20 天野エンザイム株式会社 複合型プロテイングルタミナーゼ
WO2026060197A1 (en) * 2024-09-13 2026-03-19 International N&H Denmark Aps Novel protein glutaminase for plant based beverages

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK2351837T3 (da) * 2008-09-09 2019-05-13 Amano Enzyme Inc Fremgangsmåde til at designe mutant enzym, fremgangsmåde til at fremstille mutant enzym og mutant enzym

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
A novel protein-deamidating enzyme from Chryseobacterium proteolyticum sp. nov., a newly isolated bacterium from soil. Applied and Environmental Microbiology 2000; 66(8): 3337-
Sanger et al. (1977), Proc. Natl. Acad. Sci. USA 74: 5463
Tatiana A. Tatsusova, Thomas L. Madden, FEMS Microbiol. Lett., Band 174, Seiten 247-250, 1999

Also Published As

Publication number Publication date
JPWO2023171778A1 (https=) 2023-09-14
WO2023171778A1 (ja) 2023-09-14
US20250188435A1 (en) 2025-06-12
EP4491731A1 (en) 2025-01-15

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