DE10338531A1 - Verfahren zur Reklonierung von Produktionszellen - Google Patents

Verfahren zur Reklonierung von Produktionszellen Download PDF

Info

Publication number
DE10338531A1
DE10338531A1 DE10338531A DE10338531A DE10338531A1 DE 10338531 A1 DE10338531 A1 DE 10338531A1 DE 10338531 A DE10338531 A DE 10338531A DE 10338531 A DE10338531 A DE 10338531A DE 10338531 A1 DE10338531 A1 DE 10338531A1
Authority
DE
Germany
Prior art keywords
cells
cell
mammalian
feeder
serum
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
DE10338531A
Other languages
German (de)
English (en)
Inventor
Jürgen FIEDER
Thomas Krieg
Ralf Otto
Barbara Dr. Enenkel
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Boehringer Ingelheim Pharma GmbH and Co KG
Boehringer Ingelheim Pharmaceuticals Inc
Original Assignee
Boehringer Ingelheim Pharma GmbH and Co KG
Boehringer Ingelheim Pharmaceuticals Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boehringer Ingelheim Pharma GmbH and Co KG, Boehringer Ingelheim Pharmaceuticals Inc filed Critical Boehringer Ingelheim Pharma GmbH and Co KG
Priority to DE10338531A priority Critical patent/DE10338531A1/de
Priority to ES04764194T priority patent/ES2317023T3/es
Priority to PCT/EP2004/009204 priority patent/WO2005019442A2/de
Priority to CA2536239A priority patent/CA2536239C/en
Priority to KR1020067003371A priority patent/KR101184572B1/ko
Priority to DE502004008542T priority patent/DE502004008542D1/de
Priority to JP2006523593A priority patent/JP5129959B2/ja
Priority to ES08151018T priority patent/ES2368772T3/es
Priority to EP08151018A priority patent/EP1918364B1/de
Priority to DK04764194T priority patent/DK1658365T3/da
Priority to AT04764194T priority patent/ATE415471T1/de
Priority to DK08151018.2T priority patent/DK1918364T3/da
Priority to AT08151018T priority patent/ATE515565T1/de
Priority to EP04764194A priority patent/EP1658365B1/de
Priority to TW093124872A priority patent/TWI337201B/zh
Priority to US10/922,577 priority patent/US7833787B2/en
Publication of DE10338531A1 publication Critical patent/DE10338531A1/de
Priority to US12/899,914 priority patent/US8669109B2/en
Ceased legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/0693Tumour cells; Cancer cells
    • C12N5/0694Cells of blood, e.g. leukemia cells, myeloma cells
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/0693Tumour cells; Cancer cells
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00Specific components of cell culture medium
    • C12N2500/90Serum-free medium, which may still contain naturally-sourced components
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00Coculture with; Conditioned medium produced by
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00Coculture with; Conditioned medium produced by
    • C12N2502/30Coculture with; Conditioned medium produced by tumour cells
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00Genetically modified cells
    • C12N2510/02Cells for production

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Oncology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
DE10338531A 2003-08-19 2003-08-19 Verfahren zur Reklonierung von Produktionszellen Ceased DE10338531A1 (de)

Priority Applications (17)

Application Number Priority Date Filing Date Title
DE10338531A DE10338531A1 (de) 2003-08-19 2003-08-19 Verfahren zur Reklonierung von Produktionszellen
DK04764194T DK1658365T3 (da) 2003-08-19 2004-08-17 Fremgangsmåde til genkloning af produktionsceller
AT04764194T ATE415471T1 (de) 2003-08-19 2004-08-17 Verfahren zur reklonierung von produktionszellen
CA2536239A CA2536239C (en) 2003-08-19 2004-08-17 Method for recloning production cells
KR1020067003371A KR101184572B1 (ko) 2003-08-19 2004-08-17 생산 세포를 재클로닝하는 방법
DE502004008542T DE502004008542D1 (de) 2003-08-19 2004-08-17 Verfahren zur reklonierung von produktionszellen
JP2006523593A JP5129959B2 (ja) 2003-08-19 2004-08-17 生産細胞の再クローン化方法
ES08151018T ES2368772T3 (es) 2003-08-19 2004-08-17 Método para reclonar células de producción.
EP08151018A EP1918364B1 (de) 2003-08-19 2004-08-17 Verfahren zur Reklonierung von Produktionszellen
ES04764194T ES2317023T3 (es) 2003-08-19 2004-08-17 Metodo para reclonar celulas de produccion.
PCT/EP2004/009204 WO2005019442A2 (de) 2003-08-19 2004-08-17 Verfahren zur reklonierung von produktionszellen
DK08151018.2T DK1918364T3 (da) 2003-08-19 2004-08-17 Fremgangsmåde til rekloning af produktionsceller
AT08151018T ATE515565T1 (de) 2003-08-19 2004-08-17 Verfahren zur reklonierung von produktionszellen
EP04764194A EP1658365B1 (de) 2003-08-19 2004-08-17 Verfahren zur reklonierung von produktionszellen
TW093124872A TWI337201B (en) 2003-08-19 2004-08-18 Method for recloning production cells
US10/922,577 US7833787B2 (en) 2003-08-19 2004-08-19 Method for recloning Chinese hamster ovary (CHO) cells
US12/899,914 US8669109B2 (en) 2003-08-19 2010-10-07 Methods of producing proteins in Chinese hamster ovary (CHO) cells

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE10338531A DE10338531A1 (de) 2003-08-19 2003-08-19 Verfahren zur Reklonierung von Produktionszellen

Publications (1)

Publication Number Publication Date
DE10338531A1 true DE10338531A1 (de) 2005-04-07

Family

ID=34201814

Family Applications (2)

Application Number Title Priority Date Filing Date
DE10338531A Ceased DE10338531A1 (de) 2003-08-19 2003-08-19 Verfahren zur Reklonierung von Produktionszellen
DE502004008542T Expired - Lifetime DE502004008542D1 (de) 2003-08-19 2004-08-17 Verfahren zur reklonierung von produktionszellen

Family Applications After (1)

Application Number Title Priority Date Filing Date
DE502004008542T Expired - Lifetime DE502004008542D1 (de) 2003-08-19 2004-08-17 Verfahren zur reklonierung von produktionszellen

Country Status (11)

Country Link
US (1) US7833787B2 (cg-RX-API-DMAC7.html)
EP (2) EP1658365B1 (cg-RX-API-DMAC7.html)
JP (1) JP5129959B2 (cg-RX-API-DMAC7.html)
KR (1) KR101184572B1 (cg-RX-API-DMAC7.html)
AT (2) ATE515565T1 (cg-RX-API-DMAC7.html)
CA (1) CA2536239C (cg-RX-API-DMAC7.html)
DE (2) DE10338531A1 (cg-RX-API-DMAC7.html)
DK (2) DK1658365T3 (cg-RX-API-DMAC7.html)
ES (2) ES2317023T3 (cg-RX-API-DMAC7.html)
TW (1) TWI337201B (cg-RX-API-DMAC7.html)
WO (1) WO2005019442A2 (cg-RX-API-DMAC7.html)

Families Citing this family (47)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060223183A1 (en) * 2005-03-11 2006-10-05 Bates Steven E Gamma irradiation of frozen feeder cells
EP1901068A1 (en) * 2006-09-15 2008-03-19 Boehringer Ingelheim Pharma GmbH & Co. KG Methods of selecting cell clones
US20080268470A1 (en) * 2006-09-15 2008-10-30 Hitto Kaufmann Methods of selecting cell clones
EP2257620A1 (de) * 2008-03-19 2010-12-08 Boehringer Ingelheim Pharma GmbH & Co. KG Methode zur steigerung der reklonierungseffizienz
JP5304275B2 (ja) * 2009-01-29 2013-10-02 Jnc株式会社 アポクライティン−iiをコードするコドン最適化核酸およびその使用方法
US8597943B2 (en) 2009-04-09 2013-12-03 Cellca Gmbh Method for improved single cell cloning
JP5697042B2 (ja) * 2009-11-19 2015-04-08 国立大学法人 岡山大学 遺伝子発現を上昇させるシステム及び該システムを保持したベクター
PE20141568A1 (es) 2011-10-28 2014-11-21 Neotope Biosciences Ltd Anticuerpos humanizados que reconocen la alfa-sinucleina
AU2013211874B2 (en) 2012-01-27 2017-11-02 Prothena Biosciences Limited Humanized antibodies that recognize alpha-synuclein
UA118441C2 (uk) 2012-10-08 2019-01-25 Протена Біосаєнсиз Лімітед Антитіло, що розпізнає альфа-синуклеїн
CU24446B1 (es) 2013-03-13 2019-10-04 Prothena Biosciences Ltd Un anticuerpo monoclonal humanizado que se une a tau
US10513555B2 (en) 2013-07-04 2019-12-24 Prothena Biosciences Limited Antibody formulations and methods
WO2015004632A1 (en) 2013-07-12 2015-01-15 Neotope Biosciences Limited Antibodies that recognize iapp
US9951131B2 (en) 2013-07-12 2018-04-24 Prothena Biosciences Limited Antibodies that recognize IAPP
WO2015075635A2 (en) 2013-11-19 2015-05-28 Prothena Biosciences Limited Monitoring immunotherapy of lewy body disease from constipation symptoms
TW201623331A (zh) 2014-03-12 2016-07-01 普羅帝納生物科學公司 抗黑色素瘤細胞黏著分子(mcam)抗體類及使用彼等之相關方法
US20170158755A1 (en) 2014-03-12 2017-06-08 Prothena Biosciences Limited Anti-laminin4 antibodies specific for lg1-3
WO2015136472A1 (en) 2014-03-12 2015-09-17 Prothena Biosciences Limited Anti-laminin4 antibodies specific for lg4-5
DK3116911T3 (da) 2014-03-12 2019-09-30 Prothena Biosciences Ltd Anti-mcam-antistoffer og tilhørende fremgangsmåder til anvendelse
EP3129051A1 (en) 2014-04-08 2017-02-15 Prothena Biosciences Limited Blood-brain barrier shuttles containing antibodies recognizing alpha-synuclein
TWI786505B (zh) 2015-01-28 2022-12-11 愛爾蘭商普羅佘納生物科技有限公司 抗甲狀腺素運送蛋白抗體
TWI718121B (zh) 2015-01-28 2021-02-11 愛爾蘭商普羅佘納生物科技有限公司 抗甲狀腺素運送蛋白抗體
TWI769570B (zh) 2015-01-28 2022-07-01 愛爾蘭商普羅佘納生物科技有限公司 抗甲狀腺素運送蛋白抗體
JP2018530540A (ja) 2015-09-16 2018-10-18 プロセナ バイオサイエンシーズ リミテッド 巨細胞動脈炎、リウマチ性多発筋痛症又は高安動脈炎の処置又は予防のための抗mcam抗体の使用
WO2017046774A2 (en) 2015-09-16 2017-03-23 Prothena Biosciences Limited Use of anti-mcam antibodies for treatment or prophylaxis of giant cell arteritis, polymyalgia rheumatica or takayasu's arteritis
WO2017149513A1 (en) 2016-03-03 2017-09-08 Prothena Biosciences Limited Anti-mcam antibodies and associated methods of use
WO2017153955A1 (en) 2016-03-09 2017-09-14 Prothena Biosciences Limited Use of anti-mcam antibodies for treatment or prophylaxis of granulomatous lung diseases
WO2017153953A1 (en) 2016-03-09 2017-09-14 Prothena Biosciences Limited Use of anti-mcam antibodies for treatment or prophylaxis of granulomatous lung diseases
BR112018072394A2 (pt) 2016-05-02 2019-02-19 Prothena Biosciences Limited anticorpos que reconhecem a tau
ES2933491T3 (es) 2016-05-02 2023-02-09 Prothena Biosciences Ltd Inmunoterapia tau
US10889638B2 (en) 2016-05-02 2021-01-12 Prothena Biosciences Limited Antibodies recognizing tau
WO2017208210A1 (en) 2016-06-03 2017-12-07 Prothena Biosciences Limited Anti-mcam antibodies and associated methods of use
WO2018007922A2 (en) 2016-07-02 2018-01-11 Prothena Biosciences Limited Anti-transthyretin antibodies
EP3478715A2 (en) 2016-07-02 2019-05-08 Prothena Biosciences Limited Anti-transthyretin antibodies
WO2018007924A2 (en) 2016-07-02 2018-01-11 Prothena Biosciences Limited Anti-transthyretin antibodies
US11958896B2 (en) 2017-05-02 2024-04-16 Prothena Biosciences Limited Antibodies recognizing tau
WO2019064053A1 (en) 2017-09-28 2019-04-04 Prothena Biosciences Limited DOSAGE REGIMES FOR THE TREATMENT OF SYNUCLEINOPATHIES
EP3691626A4 (en) 2017-10-06 2021-06-30 Prothena Biosciences Limited METHODS OF DETECTION OF TRANSTHYRETINE
PH12020550694A1 (en) 2017-11-29 2021-04-12 Prothena Biosciences Ltd Lyophilized formulation of a monoclonal antibody against transthyretin
CN119409813A (zh) 2018-11-08 2025-02-11 普罗塞纳生物科学有限公司 识别tau的抗体
UA128431C2 (uk) 2018-11-26 2024-07-10 Форті Севен, Інк. ГУМАНІЗОВАНЕ АНТИТІЛО ДО c-Kit
PE20212324A1 (es) 2019-03-03 2021-12-14 Prothena Biosciences Ltd Anticuerpos que reconocen tau
US20230235048A1 (en) 2020-06-24 2023-07-27 Andriani IOANNOU Antibodies recognizing sortilin
AR133829A1 (es) 2023-09-15 2025-11-05 Othair Prothena Ltd Agentes penetrantes de células y usos de los mismos
TW202530253A (zh) 2023-09-15 2025-08-01 愛爾蘭商普羅希那生物科學有限公司 抗tdp-43抗體及其用途
AR133834A1 (es) 2023-09-15 2025-11-05 Prothena Biosciences Ltd Agentes de penetración célular y sus usos
WO2025059491A2 (en) 2023-09-15 2025-03-20 Prothena Platform Technologies Limited Methods, compositions, and kits including cell-penetrating agents

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3584341D1 (de) 1984-08-24 1991-11-14 Upjohn Co Rekombinante dna-verbindungen und expression von polypeptiden wie tpa.
US5008198A (en) 1984-09-14 1991-04-16 Scripps Clinic And Research Foundation Merozoite surface glycoproteins
EP0557459B1 (en) 1990-11-13 1997-10-22 Immunex Corporation Bifunctional selectable fusion genes
US5459058A (en) * 1991-03-28 1995-10-17 Benjamin Rich Cell culture system
CA2163427A1 (en) 1993-05-21 1994-12-08 Stephen D. Lupton Bifunctional selectable fusion genes based on the cytosine deaminase (cd) gene
DE19539493A1 (de) 1995-10-24 1997-04-30 Thomae Gmbh Dr K Starker homologer Promotor aus Hamster
CN1299409A (zh) 1998-04-17 2001-06-13 雀巢制品公司 由正常人皮肤组织衍生的无限增殖细胞系
WO2000034326A1 (en) 1998-12-11 2000-06-15 Clontech Laboratories, Inc. Fluorescent proteins from non-bioluminescent species of class anthozoa, genes encoding such proteins and uses thereof
WO2000034325A1 (en) 1998-12-11 2000-06-15 Clontech Laboratories, Inc. Fluorescent proteins from non-bioluminescent species of class anthozoa, genes encoding such proteins and uses thereof
EP1135532B1 (en) 1998-12-11 2011-03-16 Clontech Laboratories Inc. Fluorescent proteins from non-bioluminescent species of class anthozoa, genes encoding such proteins and uses thereof
JP2003504059A (ja) 1999-07-12 2003-02-04 ジェネンテック・インコーポレーテッド 発現ベクターと方法
AU780810B2 (en) * 1999-08-05 2005-04-21 Baxalta GmbH Recombinant stable cell clone, its production and use thereof
US7456017B2 (en) * 1999-10-01 2008-11-25 University Of North Carolina At Chapel Hill Processes for clonal growth of hepatic progenitor cells
AU783767B2 (en) 1999-10-14 2005-12-01 Takara Bio Usa, Inc. Anthozoa derived chromophores/fluorophores and methods for using the same
JP2004510434A (ja) * 2000-10-03 2004-04-08 ユニバーシティ オブ ノース カロライナ 肝前駆細胞のクローン増殖方法
US20030073234A1 (en) * 2001-10-12 2003-04-17 Michal Amit Clonal human embryonic stem cell lines and methods of generating same

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
Acta Tropica 1981, Vol. 38, No. 3, S. 241-250 *
Biology of Reproduktion 2000, Vol. 62, No Supplement, S. 230 *
Int. J. Hyperthermia 1989, Vol. 5, No. 1, S. 99-104 *

Also Published As

Publication number Publication date
CA2536239C (en) 2014-05-27
TWI337201B (en) 2011-02-11
WO2005019442A2 (de) 2005-03-03
US20050059146A1 (en) 2005-03-17
KR101184572B1 (ko) 2012-09-21
TW200516148A (en) 2005-05-16
EP1918364A3 (de) 2008-05-14
JP2007502608A (ja) 2007-02-15
EP1918364A2 (de) 2008-05-07
EP1658365A2 (de) 2006-05-24
EP1658365B1 (de) 2008-11-26
ATE415471T1 (de) 2008-12-15
US7833787B2 (en) 2010-11-16
ATE515565T1 (de) 2011-07-15
WO2005019442A3 (de) 2005-10-06
JP5129959B2 (ja) 2013-01-30
ES2368772T3 (es) 2011-11-22
CA2536239A1 (en) 2005-03-03
EP1918364B1 (de) 2011-07-06
DE502004008542D1 (de) 2009-01-08
DK1658365T3 (da) 2009-02-02
DK1918364T3 (da) 2011-08-29
ES2317023T3 (es) 2009-04-16
KR20060065706A (ko) 2006-06-14

Similar Documents

Publication Publication Date Title
EP1658365B1 (de) Verfahren zur reklonierung von produktionszellen
JP2007502608A6 (ja) 生産細胞の再クローン化方法
US10655156B2 (en) Overexpression of N-glycosylation pathway regulators to modulate glycosylation of recombinant proteins
EP2049671A1 (de) Regulatorische nukleinsäureelemente
US11299760B2 (en) Use of monensin to regulate glycosylation of recombinant proteins
EP1567648A1 (de) Expressionsvektor, verfahren zur herstellung von heterologen genprodukten und selektionsverfahren für hochproduzierende rekombinante zellen
US8669109B2 (en) Methods of producing proteins in Chinese hamster ovary (CHO) cells
US8597909B2 (en) Use of HSA-producing cells
WO2009115495A1 (de) Methode zur steigerung der reklonierungseffizienz
HK1157387B (en) Use of hsa-producing cells
EA047078B1 (ru) Сверхэкспрессия регуляторов пути n-гликозилирования для модуляции гликозилирования рекомбинантных белков

Legal Events

Date Code Title Description
OP8 Request for examination as to paragraph 44 patent law
8131 Rejection