DE10231543B3 - Konfokale 3D-Scanning Absorption - Google Patents

Konfokale 3D-Scanning Absorption Download PDF

Info

Publication number
DE10231543B3
DE10231543B3 DE2002131543 DE10231543A DE10231543B3 DE 10231543 B3 DE10231543 B3 DE 10231543B3 DE 2002131543 DE2002131543 DE 2002131543 DE 10231543 A DE10231543 A DE 10231543A DE 10231543 B3 DE10231543 B3 DE 10231543B3
Authority
DE
Germany
Prior art keywords
focus
measuring beam
sample
transmission
change
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE2002131543
Other languages
German (de)
English (en)
Inventor
Erwin Dr. Thiel
Rainer Dipl.-Chem. Bornemann
Ingo Gregor
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bornemann Rainer 76571 Gaggenau De
Original Assignee
Universitaet Siegen
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Universitaet Siegen filed Critical Universitaet Siegen
Priority to DE2002131543 priority Critical patent/DE10231543B3/de
Priority to PCT/EP2003/007290 priority patent/WO2004008217A1/fr
Priority to AU2003246655A priority patent/AU2003246655A1/en
Application granted granted Critical
Publication of DE10231543B3 publication Critical patent/DE10231543B3/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • GPHYSICS
    • G02OPTICS
    • G02BOPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00Microscopes
    • G02B21/0004Microscopes specially adapted for specific applications
    • G02B21/002Scanning microscopes
    • G02B21/0024Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/0052Optical details of the image generation
    • G02B21/0076Optical details of the image generation arrangements using fluorescence or luminescence
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/59Transmissivity
    • G01N21/5907Densitometers
    • G01N21/5911Densitometers of the scanning type
    • GPHYSICS
    • G02OPTICS
    • G02BOPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00Microscopes
    • G02B21/0004Microscopes specially adapted for specific applications
    • G02B21/002Scanning microscopes
    • G02B21/0024Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/0032Optical details of illumination, e.g. light-sources, pinholes, beam splitters, slits, fibers
    • GPHYSICS
    • G02OPTICS
    • G02BOPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00Microscopes
    • G02B21/0004Microscopes specially adapted for specific applications
    • G02B21/002Scanning microscopes
    • G02B21/0024Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/0052Optical details of the image generation
    • G02B21/006Optical details of the image generation focusing arrangements; selection of the plane to be imaged
    • GPHYSICS
    • G02OPTICS
    • G02BOPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00Microscopes
    • G02B21/0004Microscopes specially adapted for specific applications
    • G02B21/002Scanning microscopes
    • G02B21/0024Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/008Details of detection or image processing, including general computer control

Landscapes

  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Optics & Photonics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Computer Vision & Pattern Recognition (AREA)
  • General Engineering & Computer Science (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
DE2002131543 2002-07-11 2002-07-11 Konfokale 3D-Scanning Absorption Expired - Fee Related DE10231543B3 (de)

Priority Applications (3)

Application Number Priority Date Filing Date Title
DE2002131543 DE10231543B3 (de) 2002-07-11 2002-07-11 Konfokale 3D-Scanning Absorption
PCT/EP2003/007290 WO2004008217A1 (fr) 2002-07-11 2003-07-08 Absorption confocale lors d'un balayage tridimensionnel
AU2003246655A AU2003246655A1 (en) 2002-07-11 2003-07-08 Confocal 3d-scanning absorption

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE2002131543 DE10231543B3 (de) 2002-07-11 2002-07-11 Konfokale 3D-Scanning Absorption

Publications (1)

Publication Number Publication Date
DE10231543B3 true DE10231543B3 (de) 2004-02-26

Family

ID=30009923

Family Applications (1)

Application Number Title Priority Date Filing Date
DE2002131543 Expired - Fee Related DE10231543B3 (de) 2002-07-11 2002-07-11 Konfokale 3D-Scanning Absorption

Country Status (3)

Country Link
AU (1) AU2003246655A1 (fr)
DE (1) DE10231543B3 (fr)
WO (1) WO2004008217A1 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102006046369A1 (de) * 2006-09-29 2008-04-03 Carl Zeiss Microimaging Gmbh Auflösungsgesteigerte Lumineszenzmikroskopie

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1944600B1 (fr) * 2005-07-22 2016-01-27 Carl Zeiss Microscopy GmbH Microscope à balayage laser
US9341515B2 (en) 2011-02-11 2016-05-17 University Of Central Florida Research Foundation, Inc. Optical absorbance measurement apparatus, method, and applications
CN103115900A (zh) * 2013-01-21 2013-05-22 合肥知常光电科技有限公司 一种探测固体材料表面及亚表面光学吸收的方法及装置

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4631581A (en) * 1984-03-15 1986-12-23 Sarastro Ab Method and apparatus for microphotometering microscope specimens
DE4040441A1 (de) * 1990-12-18 1992-07-02 Hell Stefan Doppelkonfokales rastermikroskop
WO1992018850A1 (fr) * 1991-04-10 1992-10-29 Mayo Foundation For Medical Education And Research Systeme d'imagerie a foyer commun pour la lumiere visible et la lumiere ultraviolette
DE4324681A1 (de) * 1993-07-22 1995-03-30 Hell Stefan Verfahren und Vorrichtung zum optischen Anregen eines Energiezustandes einer Probe in einem Probenpunkt mit hoher Ortsauflösung
DE4331570C2 (de) * 1993-08-17 1996-10-24 Hell Stefan Verfahren zum optischen Anregen einer Probe
DE4416558C2 (de) * 1994-02-01 1997-09-04 Hell Stefan Verfahren zum optischen Messen eines Probenpunkts einer Probe und Vorrichtung zur Durchführung des Verfahrens

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9014570D0 (en) * 1990-06-29 1990-08-22 Dixon Arthur E Method for transmitted-light and reflected-light imaging

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4631581A (en) * 1984-03-15 1986-12-23 Sarastro Ab Method and apparatus for microphotometering microscope specimens
DE4040441A1 (de) * 1990-12-18 1992-07-02 Hell Stefan Doppelkonfokales rastermikroskop
WO1992018850A1 (fr) * 1991-04-10 1992-10-29 Mayo Foundation For Medical Education And Research Systeme d'imagerie a foyer commun pour la lumiere visible et la lumiere ultraviolette
DE4324681A1 (de) * 1993-07-22 1995-03-30 Hell Stefan Verfahren und Vorrichtung zum optischen Anregen eines Energiezustandes einer Probe in einem Probenpunkt mit hoher Ortsauflösung
DE4331570C2 (de) * 1993-08-17 1996-10-24 Hell Stefan Verfahren zum optischen Anregen einer Probe
DE4416558C2 (de) * 1994-02-01 1997-09-04 Hell Stefan Verfahren zum optischen Messen eines Probenpunkts einer Probe und Vorrichtung zur Durchführung des Verfahrens

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102006046369A1 (de) * 2006-09-29 2008-04-03 Carl Zeiss Microimaging Gmbh Auflösungsgesteigerte Lumineszenzmikroskopie
US8399857B2 (en) 2006-09-29 2013-03-19 Carl Zeiss Microscopy Gmbh Luminescence microscopy with enhanced resolution

Also Published As

Publication number Publication date
WO2004008217A1 (fr) 2004-01-22
AU2003246655A1 (en) 2004-02-02

Similar Documents

Publication Publication Date Title
DE60109128T2 (de) Vorrichtung zur Bestimmung des Volumens einer einzigen roten Blutzelle
DE60133383T2 (de) Verfahren und Vorrichtung zur Oberflächenplasmonenmikroskopie
DE2446032A1 (de) Verfahren und vorrichtung zur feststellung submikrometrisch bemessener partikel
US20080038835A1 (en) Reference Member for Fluorescence Measurements, and Method for the Production Thereof
WO2002008732A1 (fr) Procede et dispositif d"analyse de coincidence par fluorescence a deux photons polychrome
DE102008049886A1 (de) Vorrichtung, insbesondere ein Mikroskop, zur Untersuchung von Proben
EP2233913A1 (fr) Standard optique destiné au calibrage et à la caractérisation de dispositifs de mesure optique
EP0979402B1 (fr) Procede de detection optique de molecules a analyser dans un milieu biologique naturel
DE102009003548A1 (de) Verfahren zur hochaufgelösten Erfassung von Nanopartikeln auf zweidimensionalen Messflächen
EP1678547B1 (fr) Dispositif et procede de mesure des proprietes optiques d'un objet
DE102008040513B4 (de) Verwendung einer langwellig emittierenden Cyaninverbindung als NIR-Fluoreszenzstandard und Kit zur Kalibrierung von Photolumineszenzmesssystemen
DE10231543B3 (de) Konfokale 3D-Scanning Absorption
DE102013022026A1 (de) Mehrfarben-Scanning-Mikroskop
DE60111010T2 (de) Verfahren zur Bestimmung des Volumens einer einzigen roten Blutzelle
EP2988950B9 (fr) Procédé d'étalonnage et procédé permettant de déterminer rapidement l'intensité absolue de la luminescence
DE19822452C2 (de) Verfahren zur Bestimmung der Dichte lumineszierender Moleküle an einer Oberfläche, Verwendung des Verfahrens zur Bestimmung von Adsorptions- und Bindungskinetiken und Gleichgewichts- und Bindungskonstanten von Molekülen an einer Oberfläche durch Lumineszenz-Messungen und Vorrichtung zur Durchführung des Verfahrens
DE102012214932B4 (de) Testprobenvorrichtung und Testverfahren für ein optisches, im Sub-Wellenlängenbereich auflösendes Mikroskop
EP2261641A2 (fr) Dispositif et procédé de détermination du rendement quantique de la luminescence d'un échantillon luminescent
WO2022136205A1 (fr) Dispositif et procédé de mesure de fluorescence pour une analyse d'un échantillon biochimique
CN112557363A (zh) 一种基于飞秒激光调制相位的单粒子快速识别装置及方法
DE60204735T2 (de) Verfahren zum Kalibrieren der Probenhöhe in einem Analysegerät
Kato et al. Near-field absorption imaging by a Raman nano-light source
DE4104014A1 (de) Verfahren zur bestimmung von calciumionenkonzentration in zellen
Carriles et al. Confocal and multiphoton microscopy
EP0950893A2 (fr) Dispositif pour la détection d'un colorant fluorescent

Legal Events

Date Code Title Description
8100 Publication of the examined application without publication of unexamined application
8364 No opposition during term of opposition
8327 Change in the person/name/address of the patent owner

Owner name: BORNEMANN, RAINER, 76571 GAGGENAU, DE

8320 Willingness to grant licenses declared (paragraph 23)
R082 Change of representative

Representative=s name: DENNEMEYER & ASSOCIATES S.A., DE

R082 Change of representative

Representative=s name: DENNEMEYER & ASSOCIATES S.A., DE

R119 Application deemed withdrawn, or ip right lapsed, due to non-payment of renewal fee

Effective date: 20140201