CY1109847T1 - Μεθοδος για τη διευκολυνση ανακτησης ετερολογων πολυπεπτιδιων απο βακτηριδιακα κυτταρα - Google Patents

Μεθοδος για τη διευκολυνση ανακτησης ετερολογων πολυπεπτιδιων απο βακτηριδιακα κυτταρα

Info

Publication number
CY1109847T1
CY1109847T1 CY20091100188T CY091100188T CY1109847T1 CY 1109847 T1 CY1109847 T1 CY 1109847T1 CY 20091100188 T CY20091100188 T CY 20091100188T CY 091100188 T CY091100188 T CY 091100188T CY 1109847 T1 CY1109847 T1 CY 1109847T1
Authority
CY
Cyprus
Prior art keywords
nucleic acid
cells
polypeptides
acid encoding
bacteridic
Prior art date
Application number
CY20091100188T
Other languages
English (en)
Inventor
Woon-Lam Susan Leung
James R Swartz
Original Assignee
Genentech, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genentech, Inc. filed Critical Genentech, Inc.
Publication of CY1109847T1 publication Critical patent/CY1109847T1/el

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2462—Lysozyme (3.2.1.17)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/65—Insulin-like growth factors, i.e. somatomedins, e.g. IGF-1, IGF-2
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2839—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the integrin superfamily
    • C07K16/2845—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the integrin superfamily against integrin beta2-subunit-containing molecules, e.g. CD11, CD18
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/06—Lysis of microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
    • C07K2317/24—Immunoglobulins specific features characterized by taxonomic origin containing regions, domains or residues from different species, e.g. chimeric, humanized or veneered
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/54—F(ab')2
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2795/00—Bacteriophages
    • C12N2795/00011—Details
    • C12N2795/10011—Details dsDNA Bacteriophages
    • C12N2795/10211—Podoviridae
    • C12N2795/10222—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2795/00—Bacteriophages
    • C12N2795/00011—Details
    • C12N2795/10011—Details dsDNA Bacteriophages
    • C12N2795/10311—Siphoviridae
    • C12N2795/10322—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Virology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Diabetes (AREA)
  • Toxicology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Endocrinology (AREA)
  • Mycology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Epoxy Compounds (AREA)

Abstract

Η παρούσα εφεύρεση σχετίζεται με διαδικασίες για ανάκτηση ετερόλογων πολυπεπτιδίων από βακτηριδιακά κύτταρα, συμπεριλαμβανομένου του περιπλάσματος και του κυτταροπλάσματος. Οι διαδικασίες ενέχουν καλλιέργεια των βακτηριδιακών κυττάρων,τα οποία κύτταρα περιλαμβάνουν νουκλεϊνικό οξύ που κωδικοποιεί λυσοζύμη του φάγου και νουκλεϊνικό οξύ που κωδικοποιεί μία πρωτεΐνη η οποία επιδεικνύει ενεργότητα πέψης του DNA, όπου αυτά τα νουκλεϊνικά οξέα είναι συνδεδεμένα σε έναν πρώτο προαγωγέα, και νουκλεϊνικό οξύ που κωδικοποιεί το ετερόλογο πολυπεπτίδιο, το οποίο νουκλεϊνικό οξύ είναι συνδεδεμένο σε έναν δεύτερο προαγωγέα, υπό συγκεκριμένες συνθήκες ώστε να παράγει ένα κυτταρόλυμα ζωμού· και την ανάκτηση συσσωρευμένου ετερόλογου πολυπεπτιδίου από το κυτταρόλυμα του ζωμού.
CY20091100188T 1998-10-28 2009-02-18 Μεθοδος για τη διευκολυνση ανακτησης ετερολογων πολυπεπτιδιων απο βακτηριδιακα κυτταρα CY1109847T1 (el)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US10605298P 1998-10-28 1998-10-28
EP03006278A EP1323820B1 (en) 1998-10-28 1999-10-21 Process for facillitated recovery of heterologous proteins from bacterial cells

Publications (1)

Publication Number Publication Date
CY1109847T1 true CY1109847T1 (el) 2014-09-10

Family

ID=22309219

Family Applications (1)

Application Number Title Priority Date Filing Date
CY20091100188T CY1109847T1 (el) 1998-10-28 2009-02-18 Μεθοδος για τη διευκολυνση ανακτησης ετερολογων πολυπεπτιδιων απο βακτηριδιακα κυτταρα

Country Status (13)

Country Link
US (2) US6180367B1 (el)
EP (2) EP1124941B1 (el)
JP (1) JP3711326B2 (el)
AT (2) ATE415469T1 (el)
AU (1) AU752429B2 (el)
CA (1) CA2346797C (el)
CY (1) CY1109847T1 (el)
DE (2) DE69911450T2 (el)
DK (2) DK1323820T3 (el)
ES (2) ES2207340T3 (el)
IL (2) IL142269A0 (el)
PT (2) PT1323820E (el)
WO (1) WO2000024873A1 (el)

Families Citing this family (38)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MXPA00009561A (es) * 1998-03-30 2002-08-06 Metabolix Inc Cepas microbianas y procesos para la fabricacion de biomateriales.
US7858339B1 (en) 1998-10-28 2010-12-28 Genentech, Inc. Process for bacterial production of polypeptides
AU4305501A (en) * 1999-12-30 2001-07-16 Intel Corporation Optimized driver layout for integrated circuits with staggered bond pads
US6784558B2 (en) * 1999-12-30 2004-08-31 Intel Corporation Semiconductor device inlcluding optimized driver layout for integrated circuit with staggered bond pads
MXPA04001566A (es) * 2001-08-27 2004-05-17 Genentech Inc Un sistema para expresion y ensamblado de anticuerpos.
US20030198682A1 (en) * 2002-02-12 2003-10-23 Gruber James V. Composition and method for protecting labile active components during high temperature drying
US9453251B2 (en) 2002-10-08 2016-09-27 Pfenex Inc. Expression of mammalian proteins in Pseudomonas fluorescens
US7195611B1 (en) * 2002-12-31 2007-03-27 Advanced Cardiovascular Systems, Inc. Rapid exchange balloon catheter having a reinforced inner tubular member
US20060134739A1 (en) * 2003-03-11 2006-06-22 Chatterjee Deb K Synthesis of proteins by cell-free protein expression
EP2327718B1 (en) 2003-11-21 2016-03-23 Pfenex, Inc. Improved expression systems with SEC-system secretion
CN101031655A (zh) 2004-07-26 2007-09-05 陶氏环球技术公司 通过株工程改进蛋白表达的方法
BRPI0515418A (pt) * 2004-08-16 2008-07-22 Nature Technology Corp método para a produção de dna de plasmìdio super-enrolado fechado covalentemente e composição de matéria
US20060156998A1 (en) * 2005-01-19 2006-07-20 Shawn Bridy Protective collar for animals
MY143274A (en) 2005-12-22 2011-04-15 Genentech Inc Recombinant production of heparin binding proteins
US20080125580A1 (en) 2006-07-14 2008-05-29 Genentech, Inc. Refolding of Recombinant Proteins
DK2431470T3 (da) * 2006-11-30 2018-01-29 Novozymes As DNase expression i rekombinante værtsceller
KR101491867B1 (ko) * 2007-01-31 2015-02-10 피페넥스 인크. 증가된 발현을 위한 박테리아 리더 서열
US9580719B2 (en) 2007-04-27 2017-02-28 Pfenex, Inc. Method for rapidly screening microbial hosts to identify certain strains with improved yield and/or quality in the expression of heterologous proteins
WO2008134461A2 (en) 2007-04-27 2008-11-06 Dow Global Technologies, Inc. Method for rapidly screening microbial hosts to identify certain strains with improved yield and/or quality in the expression of heterologous proteins
US9017966B2 (en) * 2007-05-23 2015-04-28 Nature Technology Corporation E. coli plasmid DNA production
US20110020830A1 (en) * 2008-03-31 2011-01-27 Schneider Jane C Design for rapidly cloning one or more polypeptide chains into an expression system
ES2370947B1 (es) * 2009-12-23 2012-11-02 Consejo Superior De Investigaciones Científicas (Csic) Sistema de autolisis celular para el procesado de la biomasa bacteriana en la produccion de polihidroxialcanoatos en pseudomonas putida kt2440
KR101222889B1 (ko) 2010-02-25 2013-01-17 고려대학교 산학협력단 이중프로모터를 포함하는 발현 시스템 및 그 시스템을 이용한 유전자 과다 발현 방법
WO2013147043A1 (ja) * 2012-03-29 2013-10-03 株式会社シノテスト 核酸の検出又は定量方法及び検出又は定量用試薬キット
GB201208367D0 (en) 2012-05-14 2012-06-27 Ucb Pharma Sa Biological product
KR20160029731A (ko) 2013-03-12 2016-03-15 더 제너럴 하스피탈 코포레이션 변형된 뮐러관 억제 물질(mis) 단백질 및 질환 치료를 위한 그 용도
EP3079712B1 (en) 2013-12-11 2022-02-02 The General Hospital Corporation Use of mullerian inhibiting substance (mis) proteins for contraception and ovarian reserve preservation
JP6803328B2 (ja) * 2014-08-28 2021-01-06 シンセティック バイオロジクス，インコーポレイテッド 大腸菌ベースでのベータラクタマーゼ生産
MX2018011834A (es) * 2016-03-29 2019-03-06 Geltor Inc Expresión de proteínas en bacterias gram negativas en la que la relación del volumen periplasmático con respecto al volumen citoplasmático está entre 0.5:1 y 10:1.
US11180541B2 (en) 2017-09-28 2021-11-23 Geltor, Inc. Recombinant collagen and elastin molecules and uses thereof
GB201814590D0 (en) * 2018-09-07 2018-10-24 Oxford Biomedica Ltd Viral vector production system
JP2022024197A (ja) * 2018-09-28 2022-02-09 Ｓｐｉｂｅｒ株式会社 組換え細胞、組換え細胞の破砕方法及び目的タンパク質の製造方法
CN113993885A (zh) 2019-04-12 2022-01-28 格尔托公司 重组弹性蛋白及其生产
JP7259131B2 (ja) 2019-08-05 2023-04-17 ワッカー ケミー アクチエンゲゼルシャフト 発酵法で組換えタンパク質を放出する細菌株
EP4127205A1 (en) * 2020-04-03 2023-02-08 Novozymes A/S Submerged fermentation process
EP3926039A1 (en) * 2020-06-17 2021-12-22 AB Enzymes GmbH Use of a nuclease for reducing the viscosity and/or preventing an increase in viscosity of a fermentation broth
WO2023019197A1 (en) * 2021-08-11 2023-02-16 Duke University Rapid expression and purification of thermostable proteins including taq polymerase
WO2024133316A1 (en) * 2022-12-23 2024-06-27 Thermo Fisher Scientific Baltics Uab Closed system for expressing recombinant proteins using e. coli and methods for producing clarified cell cultures thereof

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4595658A (en) 1982-09-13 1986-06-17 The Rockefeller University Method for facilitating externalization of proteins synthesized in bacteria
US4637980A (en) 1983-08-09 1987-01-20 Smithkline Beckman Corporation Externalization of products of bacteria
EP0155189A3 (en) * 1984-03-16 1987-09-16 Genentech, Inc. Expression of cell wall degrading proteins and host cells harboring dna encoding such protein
DE3507648A1 (de) 1985-03-05 1986-09-11 Klöckner-Humboldt-Deutz AG, 5000 Köln Vorrichtung und verfahren zum warmhalten von fluessigen metallschmelzen
JPH0640832B2 (ja) 1985-05-10 1994-06-01 味の素株式会社 インタ−ロイキン２ポリペプチドの回収方法
SE8505027D0 (sv) * 1985-10-24 1985-10-24 Kabigen Ab A lytic virulent phage, a hostcell containing same and a process for protein production
US5169772A (en) 1988-06-06 1992-12-08 Massachusetts Institute Of Technology Large scale method for purification of high purity heparinase from flavobacterium heparinum
US5512456A (en) 1990-05-25 1996-04-30 James Madison University Method for the improved production and recovery of poly-β-hydroxybutyrate from transformed Escherichia coli
RU2043415C1 (ru) 1992-02-18 1995-09-10 Биотехнологическая компания "Биосервис" ВЕКТОР PEL 5b, ПРЕДНАЗНАЧЕННЫЙ ДЛЯ ЭКСПРЕССИИ ЧУЖЕРОДНОЙ ДНК
RU2071503C1 (ru) 1992-02-18 1997-01-10 Биотехнологическая компания "Биосервис" ВЕКТОР pЕL5С, ПРЕДНАЗНАЧЕННЫЙ ДЛЯ ЭКСПРЕССИИ ЧУЖЕРОДНОЙ ДНК
RU2071501C1 (ru) 1992-02-18 1997-01-10 Биотехнологическая компания "Биосервис" Вектор реl25а, предназначенный для экспрессии чужеродного днк
WO1994012214A1 (en) * 1992-12-01 1994-06-09 Protein Design Labs, Inc. Humanized antibodies reactive with cd18

Also Published As

Publication number Publication date
CA2346797A1 (en) 2000-05-04
DK1323820T3 (da) 2009-03-16
PT1124941E (pt) 2004-02-27
IL142269A0 (en) 2002-03-10
AU1219500A (en) 2000-05-15
ATE415469T1 (de) 2008-12-15
DE69911450D1 (de) 2003-10-23
ES2318070T3 (es) 2009-05-01
IL142269A (en) 2010-12-30
DE69911450T2 (de) 2004-07-22
DE69939991D1 (de) 2009-01-08
US6258560B1 (en) 2001-07-10
ATE250125T1 (de) 2003-10-15
JP2002528071A (ja) 2002-09-03
DK1124941T3 (da) 2004-02-02
EP1323820B1 (en) 2008-11-26
EP1323820A2 (en) 2003-07-02
US6180367B1 (en) 2001-01-30
PT1323820E (pt) 2009-03-05
WO2000024873A1 (en) 2000-05-04
JP3711326B2 (ja) 2005-11-02
ES2207340T3 (es) 2004-05-16
EP1124941A1 (en) 2001-08-22
WO2000024873A9 (en) 2000-11-02
EP1124941B1 (en) 2003-09-17
AU752429B2 (en) 2002-09-19
EP1323820A3 (en) 2003-07-09
CA2346797C (en) 2008-09-23

Similar Documents

Publication Publication Date Title
CY1109847T1 (el) Μεθοδος για τη διευκολυνση ανακτησης ετερολογων πολυπεπτιδιων απο βακτηριδιακα κυτταρα
ATE317398T1 (de) Modifiziertes e.coli enterotoxin ii signalpeptid sowie ein mikroorganismus der ein fusionsprotein sowie ein heterologes protein exprimiert
TW201629226A (zh) 用於胜肽生產之融合夥伴
CA2203373A1 (en) Process for improved recombinant production of disulphide bond-containing polypeptides via a protein disulfide isomerase
ATE421977T1 (de) Reinigung von peptiden unter verwendung von metall-ion-affinitätschromatographie
AU3774300A (en) Secreted and transmembrane polypeptides and nucleic acids encoding the same
CA2313248A1 (en) Process for the production of naturally folded and secreted proteins by co-secretion of molecular chaperones
EP1027437B8 (en) Uses of the wnt-1 induced secreted polypeptide wisp-1
EP1609863A3 (en) Secreted and transmembrane polypeptides and nucleic acids encoding the same
DE69739013D1 (de) Verfahren zur Herstellung sekretorischer KEX2-Derivate
ITMI930456A1 (it) Vettore ricombinante e suo impiego per la preparazione esocellulare di anticorpi in forma di singola molecola da bacillus subtilis
DE60335602D1 (de) Rekombinante Desoxyribonuclease I aus Rinder-Pankreas mit hoher spezifischer Aktivität
Wong et al. Escherichia coli: A versatile platform for recombinant protein expression
EP1010758A3 (en) An expression system for producing recombinant human erythropoietin, a method for purifying the secreted human erythropoietin and uses thereof
DK0959130T3 (da) Kloning og udtrykkelse af nyt glucoamylasegen fra Endomyces fibuliger
EP1241186A3 (en) Human growth arrest specific gene 6 (gas-6) and nucleic acids encoding the same
Balganesh et al. Vector to produce biologically important peptides
EP1300417A3 (en) Secreted and transmembrane polypeptides and nucleic acids encoding the same
ATE409225T1 (de) Durch wnt-1 induzierbare gene
EP1251173A3 (en) Secreted and transmembrane polypeptides and nucleic acids encoding the same
DK1591452T3 (da) Secernerede og transmembrane polypeptider og nukleinsyrer kodende for samme
EP1217006A3 (en) Polypeptides and nucleic acids encoding the same
ATE362984T1 (de) Neuartige proteine und deren dna