CO6300961A2 - Circovirus porcino quimerico pcv2gen-1rep y usos del mismo - Google Patents

Circovirus porcino quimerico pcv2gen-1rep y usos del mismo

Info

Publication number
CO6300961A2
CO6300961A2 CO10128065A CO10128065A CO6300961A2 CO 6300961 A2 CO6300961 A2 CO 6300961A2 CO 10128065 A CO10128065 A CO 10128065A CO 10128065 A CO10128065 A CO 10128065A CO 6300961 A2 CO6300961 A2 CO 6300961A2
Authority
CO
Colombia
Prior art keywords
nucleic acid
pcv2
porcine circovirus
rep
pcv2gen
Prior art date
Application number
CO10128065A
Other languages
English (en)
Inventor
Nicole M Juhan
Xiang-Jin Meng
Original Assignee
Virginia Tech Intell Prop
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Virginia Tech Intell Prop filed Critical Virginia Tech Intell Prop
Publication of CO6300961A2 publication Critical patent/CO6300961A2/es

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C07K14/01—DNA viruses
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/12—Viral antigens
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A61P31/14—Antivirals for RNA viruses
    • A61P31/16—Antivirals for RNA viruses for influenza or rhinoviruses
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A61P31/20—Antivirals for DNA viruses
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A61P37/02—Immunomodulators
    • A61P37/04—Immunostimulants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
    • A61K2039/525—Virus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
    • C12N2750/00011—Details
    • C12N2750/10011—Circoviridae
    • C12N2750/10021—Viruses as such, e.g. new isolates, mutants or their genomic sequences

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Virology (AREA)
  • Organic Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Engineering & Computer Science (AREA)
  • Communicable Diseases (AREA)
  • Oncology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Pulmonology (AREA)
  • Mycology (AREA)
  • Epidemiology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

La presente invención se refiere a una molécula de ácido nucleico quimérico novedoso del circovirus porcino (PCV2Gen-1Rep) que comprende una molécula de ácido nucleico que codifica el circovirus porcino de tipo 2 (PCV2), que contiene una secuencia de ácido nucleico que codifica una proteína Rep del circovirus porcino de tipo 1 (PCV1), particularmente en donde la secuencia de ácido nucleico que codifica la proteína Rep de PCV1 es un gen del marco de lectura abierto (ORF) y, más particularmente, en donde el gen Rep del ORF es ORF1; se construye una molécula de ácido nucleico quimérico muy conveniente reemplazando el gen Rep de ORF1 de PCV2 con el gen Rep de ORF1 de PCV1; la invención también abarca el plásmido o vector viral biológicamente funcional que contiene las moléculas de ácido nucleico quimérico único, células hospederas adecuadas transfectadas por el plásmido o vector, circovirus porcinos quiméricos infecciosos que son producidos por las células hospederas adecuadas, el procedimiento de producción de un producto polipeptídico inmunogénico que utiliza la quimera nueva, vacunas virales que protegen a un cerdo contra la infección viral o el síndrome del desmedro multisistémico post-destete (PMWS) causados por PCV2, métodos de protección de un cerdo contra la infección viral o el síndrome del desmedro multisistémico post-destete (PMWS) causados por PCV2, métodos de preparación de la quimera única de PCV2Gen-1Rep, etcétera; además, esta invención incluye un método nuevo para mejorar la replicación y el título de PCV2 en un cultivo celular.
CO10128065A 2008-04-16 2010-10-14 Circovirus porcino quimerico pcv2gen-1rep y usos del mismo CO6300961A2 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US12438308P 2008-04-16 2008-04-16

Publications (1)

Publication Number Publication Date
CO6300961A2 true CO6300961A2 (es) 2011-07-21

Family

ID=41199387

Family Applications (1)

Application Number Title Priority Date Filing Date
CO10128065A CO6300961A2 (es) 2008-04-16 2010-10-14 Circovirus porcino quimerico pcv2gen-1rep y usos del mismo

Country Status (15)

Country Link
US (1) US20110027312A1 (es)
EP (1) EP2276507A4 (es)
JP (1) JP2011519273A (es)
KR (1) KR20100135903A (es)
CN (1) CN102065890B (es)
AU (1) AU2009236669B2 (es)
BR (1) BRPI0911200A2 (es)
CA (1) CA2721513A1 (es)
CO (1) CO6300961A2 (es)
MX (1) MX2010011353A (es)
NZ (1) NZ588639A (es)
RU (1) RU2515901C2 (es)
UA (1) UA106587C2 (es)
WO (1) WO2009128878A1 (es)
ZA (1) ZA201007401B (es)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AR078253A1 (es) 2009-09-02 2011-10-26 Boehringer Ingelheim Vetmed Metodos para reducir la actividad antivirica en composiciones pcv-2 y composiciones pcv-2 con mejor inmunogenicidad
HUP1100470A2 (en) * 2011-08-30 2013-03-28 Mezoegazdasagi Biotechnologiai Kutatokoezpont Nanoparticle-based veterinary vaccine
EP2564869A1 (en) 2011-09-02 2013-03-06 Ceva Sante Animale Synthetic capsid proteins and uses thereof
CN102716476B (zh) * 2012-05-31 2015-04-22 郑州后羿制药有限公司 一种细胞灭活疫苗和卵黄抗体注射液及其制备方法
CN102861327B (zh) * 2012-09-27 2015-01-21 郑州后羿制药有限公司 一种细胞灭活疫苗、卵黄抗体及卵黄抗体的注射液和冻干粉
BR112018007525A2 (pt) * 2015-10-16 2018-10-30 Kansas State University Research Foundation composições imunogênicas de circovírus suíno tipo 3 e métodos de produzir e usar as mesmas
CN105606804A (zh) * 2016-01-20 2016-05-25 福州大北农生物技术有限公司 一种猪圆环病毒灭活疫苗效力检验方法
CN107298700B (zh) * 2017-06-14 2020-07-28 山东省农业科学院畜牧兽医研究所 人工改造的PCV2 Rep蛋白、重组PCV2病毒及其应用
CN109535229B (zh) * 2018-11-21 2020-06-09 长江大学 重组猪圆环病毒2型Rep蛋白、ELISA检测试剂盒及其制备方法与应用
CN110041409B (zh) * 2019-05-07 2022-09-06 山东省农业科学院畜牧兽医研究所 一种突变型猪圆环病毒2型病毒及应用
CN111892659A (zh) * 2020-07-20 2020-11-06 武汉科前生物股份有限公司 一种嵌合型猪圆环病毒PCV1-2d疫苗及其制备方法和应用

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002077210A2 (en) * 2001-03-27 2002-10-03 University Of Saskatchewan Methods to culture circovirus
US7276353B2 (en) * 2001-12-12 2007-10-02 Virginia Tech Intellectual Properties, Inc. Chimeric infectious DNA clones, chimeric porcine circoviruses and uses thereof
US7279166B2 (en) * 2001-12-12 2007-10-09 Virginia Tech Intellectual Properties, Inc. Chimeric infectious DNA clones, chimeric porcine circoviruses and uses thereof
CN1693455A (zh) * 2005-04-08 2005-11-09 扬州大学 嵌合型猪圆环病毒pcv1－2及其构建方法、用途

Also Published As

Publication number Publication date
CN102065890B (zh) 2014-04-02
CA2721513A1 (en) 2009-10-22
MX2010011353A (es) 2011-02-23
BRPI0911200A2 (pt) 2016-07-05
JP2011519273A (ja) 2011-07-07
WO2009128878A1 (en) 2009-10-22
NZ588639A (en) 2012-10-26
CN102065890A (zh) 2011-05-18
UA106587C2 (uk) 2014-09-25
EP2276507A4 (en) 2011-11-16
KR20100135903A (ko) 2010-12-27
RU2515901C2 (ru) 2014-05-20
EP2276507A1 (en) 2011-01-26
ZA201007401B (en) 2011-06-29
US20110027312A1 (en) 2011-02-03
AU2009236669A1 (en) 2009-10-22
RU2010146480A (ru) 2012-05-27
AU2009236669B2 (en) 2012-12-06

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