CO6251278A2 - Metodo para la produccion de conjugados del factor de crecimiento similar a la insulina -1 y polietilenglicol - Google Patents

Metodo para la produccion de conjugados del factor de crecimiento similar a la insulina -1 y polietilenglicol

Info

Publication number
CO6251278A2
CO6251278A2 CO09008773A CO09008773A CO6251278A2 CO 6251278 A2 CO6251278 A2 CO 6251278A2 CO 09008773 A CO09008773 A CO 09008773A CO 09008773 A CO09008773 A CO 09008773A CO 6251278 A2 CO6251278 A2 CO 6251278A2
Authority
CO
Colombia
Prior art keywords
pro
igf
variant
thr
gly
Prior art date
Application number
CO09008773A
Other languages
English (en)
Inventor
Stephan Fischer
Friederike Hesse
Hendrik Knoetgen
Kurt Lang
Friedrich Metzger
Joerg Regula
Christian Schantz
Andreas Schaubmar
Hans Joachim Schoenfeld
Original Assignee
Hoffmann La Roche
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=37667695&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=CO6251278(A2) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Hoffmann La Roche filed Critical Hoffmann La Roche
Publication of CO6251278A2 publication Critical patent/CO6251278A2/es

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/65—Insulin-like growth factors, i.e. somatomedins, e.g. IGF-1, IGF-2
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
    • A61K47/51—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent
    • A61K47/56—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic macromolecular compound, e.g. an oligomeric, polymeric or dendrimeric molecule
    • A61K47/59—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic macromolecular compound, e.g. an oligomeric, polymeric or dendrimeric molecule obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds, e.g. polyureas or polyurethanes
    • A61K47/60—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic macromolecular compound, e.g. an oligomeric, polymeric or dendrimeric molecule obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds, e.g. polyureas or polyurethanes the organic macromolecular compound being a polyoxyalkylene oligomer, polymer or dendrimer, e.g. PEG, PPG, PEO or polyglycerol
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/28—Drugs for disorders of the nervous system for treating neurodegenerative disorders of the central nervous system, e.g. nootropic agents, cognition enhancers, drugs for treating Alzheimer's disease or other forms of dementia
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C07K2319/21—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a His-tag
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/50—Fusion polypeptide containing protease site

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • General Engineering & Computer Science (AREA)
  • Animal Behavior & Ethology (AREA)
  • Wood Science & Technology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Epidemiology (AREA)
  • Diabetes (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Endocrinology (AREA)
  • Neurology (AREA)
  • Plant Pathology (AREA)
  • Neurosurgery (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Psychiatry (AREA)
  • Hospice & Palliative Care (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Peptides Or Proteins (AREA)
  • Medicinal Preparation (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

1.- Un método para la producción de un IGF-I o una variante de IGF-I PEGilado en la lisina, y dicha variante comprende uno o dos aminoácido(s) seleccionados del grupo que consiste en lisina 27, 65 y/o 68, que están sustituidos independientemente por otro aminoácido polar, que se caracteriza por a) cultivar una célula hospedera procariota que comprende un vector de expresión que contiene un ácido nucleico que codifica una proteína de fusión que comprende dicho IGF-I o variante de IGF-I unida por el extremo N-terminal al C-terminal de un propéptido, b) en el que dicho propéptido finaliza en el extremo C-terminal con los aminoácidos -Y-Pro, en el que Y se selecciona del grupo que consiste en Pro, Pro-Ala, Pro-Gly, Pro-Thr, Ala-Pro, Gly-Pro, Thr-Pro, Arg-Pro, o Pro-Arg-Pro, c) recuperar y PEGilar dicha proteína de fusión, d) escindir dicha proteína de fusión PEGilada con la proteasa IgA, y e) recuperar dicho IGF-I PEGilado o variante de IGF-I. 2.- Un método de acuerdo con la reivindicación 1, que se caracteriza porque el propéptido se muestra mediante la fórmula Met-X1-Hisn-X2-Y-Pro-, en la que ° Met indica metionina ° X1 es un enlace, serina o asparagina ° His es histidina, ° n es un número de 0 a 10, ° X2 es un péptido enlazante, seleccionado de entre el grupo que consiste en los péptidos Id. de Sec. N°: 6-10, ° Pro es prolina, y ° Y se selecciona del grupo que consiste en Pro, Pro-Ala, Pro-Gly, Pro-Thr, Ala-Pro, Gly-Pro, Thr-Pro, Arg-Pro o Pro-Arg-Pro.3.- Un método de acuerdo con las reivindicaciones 1 a 2, que se caracteriza porque dicho propéptido no comprende un residuo de lisina. 4.- Un método de acuerdo con las reivindicaciones 1 a 3, que se caracteriza porque dicho(s) aminoácido(s) polar(es) es/ son independientemente arginina, glutamina o asparagina. 5.- Un método de acuerdo con las reivindicaciones 1 a 4, que se caracteriza porque la variante de IGP-I PEGilada en la lisina está mono- o diPEGilada en el(los) residuo(s) de lisina 65 y 68.
CO09008773A 2006-08-31 2009-01-30 Metodo para la produccion de conjugados del factor de crecimiento similar a la insulina -1 y polietilenglicol CO6251278A2 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP06018170 2006-08-31

Publications (1)

Publication Number Publication Date
CO6251278A2 true CO6251278A2 (es) 2011-02-21

Family

ID=37667695

Family Applications (1)

Application Number Title Priority Date Filing Date
CO09008773A CO6251278A2 (es) 2006-08-31 2009-01-30 Metodo para la produccion de conjugados del factor de crecimiento similar a la insulina -1 y polietilenglicol

Country Status (21)

Country Link
US (4) US7625996B2 (es)
EP (1) EP2059261B1 (es)
JP (1) JP5184532B2 (es)
KR (1) KR101106931B1 (es)
CN (1) CN101511390B (es)
AR (1) AR062575A1 (es)
AU (1) AU2007291502B2 (es)
BR (1) BRPI0715943A2 (es)
CA (1) CA2662062C (es)
CL (1) CL2007002502A1 (es)
CO (1) CO6251278A2 (es)
CR (1) CR10591A (es)
ES (1) ES2397660T3 (es)
IL (1) IL196736A (es)
MA (1) MA30660B1 (es)
MX (1) MX2009002011A (es)
NO (1) NO20090451L (es)
PE (1) PE20080912A1 (es)
RU (1) RU2009106118A (es)
TW (1) TW200819468A (es)
WO (1) WO2008025528A1 (es)

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Publication number Publication date
EP2059261A1 (en) 2009-05-20
CN101511390B (zh) 2013-04-24
AU2007291502B2 (en) 2012-05-31
KR20090046875A (ko) 2009-05-11
IL196736A0 (en) 2009-11-18
CA2662062C (en) 2015-06-23
US20100210547A1 (en) 2010-08-19
CN101511390A (zh) 2009-08-19
US20100035817A1 (en) 2010-02-11
MA30660B1 (fr) 2009-08-03
US20080119409A1 (en) 2008-05-22
CL2007002502A1 (es) 2008-05-30
JP5184532B2 (ja) 2013-04-17
KR101106931B1 (ko) 2012-01-25
IL196736A (en) 2013-10-31
NO20090451L (no) 2009-02-26
MX2009002011A (es) 2009-03-05
AU2007291502A1 (en) 2008-03-06
RU2009106118A (ru) 2010-10-10
US20130065831A1 (en) 2013-03-14
TW200819468A (en) 2008-05-01
CA2662062A1 (en) 2008-03-06
PE20080912A1 (es) 2008-07-19
US8476232B2 (en) 2013-07-02
US7625996B2 (en) 2009-12-01
ES2397660T3 (es) 2013-03-08
EP2059261B1 (en) 2012-10-24
JP2010501192A (ja) 2010-01-21
AR062575A1 (es) 2008-11-19
BRPI0715943A2 (pt) 2013-07-30
WO2008025528A1 (en) 2008-03-06
CR10591A (es) 2009-04-03

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