CO5050270A1 - Proceso para la produccion devirus en cultivos celulares - Google Patents

Proceso para la produccion devirus en cultivos celulares

Info

Publication number
CO5050270A1
CO5050270A1 CO99016858A CO99016858A CO5050270A1 CO 5050270 A1 CO5050270 A1 CO 5050270A1 CO 99016858 A CO99016858 A CO 99016858A CO 99016858 A CO99016858 A CO 99016858A CO 5050270 A1 CO5050270 A1 CO 5050270A1
Authority
CO
Colombia
Prior art keywords
virus
medium
cell culture
appropriate period
cells
Prior art date
Application number
CO99016858A
Other languages
English (en)
Inventor
Freire Marcos Da Silva
Anna Maya Yoshida Yamamura
George Fobes Mann
Ricardo Galler
Original Assignee
Fundacao Oswaldo Cruz
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=4070797&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=CO5050270(A1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Fundacao Oswaldo Cruz filed Critical Fundacao Oswaldo Cruz
Publication of CO5050270A1 publication Critical patent/CO5050270A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A61P31/14—Antivirals for RNA viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
    • C12N2770/00011—Details
    • C12N2770/24011—Flaviviridae
    • C12N2770/24111—Flavivirus, e.g. yellow fever virus, dengue, JEV
    • C12N2770/24151—Methods of production or purification of viral material

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Virology (AREA)
  • Wood Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Communicable Diseases (AREA)
  • Oncology (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

El objetivo de la invención es un proceso para la producción devirus en cultivos celulares con una alta producción y bajos niveles de contaminación por proteínas celulares. El virus es apropiado para ser usado en la preparación de lotes de semilla y vacunas. Como se usa aquí, el término "virus" significa virus inactivado, virus atenuado y virus recombinante, incluyendo virus quimérico. El proceso comprende las etapas de : (a) preparar un cultivo de células que es permisivo al virus y aceptable como un sustrato para producción de vacunas; (b) suspender las células en un medio adecuado seguido por un crecimiento celular en bajas densidades; c) incubar el cultivo de células entre 30 a 40° C durante un período de tiempo apropiado; d) remover el medio desde el cultivo de células de la etapa (c) e inocularlo con virus de semilla; (e) incubar el cultivo celular de la etapa (d) entre 25 a 40° C durante un período de tiempo apropiado; (f) remover el medio seguido por un lavado de las células una o varias veces y reemplazar el medio; (g) incubar el cultivo celular de la etapa (f) entre 25 a 40° C durante un período de tiempo apropiado; (h) recolectar total o parcialmente el sobrenadante del cultivo que contiene el virus con o sin la adición del estabilizador, (i) opcionalmente, llevar a cabo múltiples recolecciones del medio que contiene virus, a cualquier intervalo deseado, por medio de reemplazar el medio removido y reencubar el cultivo durante un período apropiado; (j) opcionalmente, remover los desechos de células y células completas del virus recolectado; (k) opcionalmente, inactivación del virus y; (l) almacenar el virus a -45° C o menos.
CO99016858A 1998-03-18 1999-03-18 Proceso para la produccion devirus en cultivos celulares CO5050270A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
BRPI9804283-1A BR9804283B1 (pt) 1998-03-18 1998-03-18 processo para a produção de flavivìrus em baixa densidade de células em cultura e processo para a produção de flavivìrus recombinante em baixa densidade de células em cultura.

Publications (1)

Publication Number Publication Date
CO5050270A1 true CO5050270A1 (es) 2001-06-27

Family

ID=4070797

Family Applications (1)

Application Number Title Priority Date Filing Date
CO99016858A CO5050270A1 (es) 1998-03-18 1999-03-18 Proceso para la produccion devirus en cultivos celulares

Country Status (8)

Country Link
EP (1) EP1002054B1 (es)
JP (1) JP2001527422A (es)
BR (1) BR9804283B1 (es)
CA (1) CA2288679A1 (es)
CO (1) CO5050270A1 (es)
DE (1) DE69927769T2 (es)
OA (1) OA11393A (es)
WO (1) WO1999047645A1 (es)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2014080676A1 (ja) 2012-11-22 2014-05-30 旭化成メディカル株式会社 高感染価のパルボウイルスの生産方法
GB201310031D0 (en) * 2013-06-05 2013-07-17 Pirbright Inst The Cell
WO2022119560A1 (en) * 2020-12-01 2022-06-09 National Health Research Institutes Cell strain having increased virus production ability and production method thereof

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AT393277B (de) * 1990-01-04 1991-09-25 Immuno Ag Verfahren zur herstellung von fruehsommer- meningoenzephalitis-virus (fsme-virus)-antigen
ES2026827A6 (es) * 1991-03-26 1992-05-01 Ercros Sa Procedimiento para la produccion de una vacuna subunidad contra el parvovirus porcino.
FR2689520B1 (fr) * 1992-04-03 1996-07-19 Bio Merieux Procede et milieu de culture pour l'obtention de cellules infectees par un virus associe a la sclerose en plaques.

Also Published As

Publication number Publication date
BR9804283A (pt) 2000-04-04
BR9804283B1 (pt) 2010-11-30
CA2288679A1 (en) 1999-09-23
JP2001527422A (ja) 2001-12-25
EP1002054B1 (en) 2005-10-19
WO1999047645A1 (en) 1999-09-23
DE69927769D1 (de) 2006-03-02
EP1002054A1 (en) 2000-05-24
OA11393A (en) 2004-04-08
DE69927769T2 (de) 2006-07-20

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