CO4870727A1 - Inhibicion pre y post cosecha de la transformacion de pro- ductos almacenados - Google Patents

Inhibicion pre y post cosecha de la transformacion de pro- ductos almacenados

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Publication number
CO4870727A1
CO4870727A1 CO98063669A CO98063669A CO4870727A1 CO 4870727 A1 CO4870727 A1 CO 4870727A1 CO 98063669 A CO98063669 A CO 98063669A CO 98063669 A CO98063669 A CO 98063669A CO 4870727 A1 CO4870727 A1 CO 4870727A1
Authority
CO
Colombia
Prior art keywords
plant
tfs
recombinant dna
protein
trehalose phosphate
Prior art date
Application number
CO98063669A
Other languages
English (en)
Inventor
Goddijn Oscar Johannes Maria
Dun Cornelis Maria Petrus Van
Tigelaar Hendrick
Krause Klaus-Peter
Original Assignee
Mogen Internat Nv
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Mogen Internat Nv filed Critical Mogen Internat Nv
Publication of CO4870727A1 publication Critical patent/CO4870727A1/es

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/1048Glycosyltransferases (2.4)
    • C12N9/1051Hexosyltransferases (2.4.1)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8245Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8262Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield involving plant development
    • C12N15/8267Seed dormancy, germination or sprouting
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/16Hydrolases (3) acting on ester bonds (3.1)
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146Genetically Modified [GMO] plants, e.g. transgenic plants

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Nutrition Science (AREA)
  • Medicinal Chemistry (AREA)
  • Physiology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Storage Of Fruits Or Vegetables (AREA)
  • Pretreatment Of Seeds And Plants (AREA)
  • Cultivation Of Plants (AREA)

Abstract

Un método para inhibir la removilización pre y post-cosechade compuestos almacenados en plantas mediante la transformación de una planta o de una planta obtenida a partir de su línea materna, con un ADN recombinante capaz de expresar una proteína, caracterizada en que la proteína es la trehalosa fosfato sintetasa (TFS).Un método para evitar la germinación de una parte de una planta mediante la transformación de la planta o de una planta derivada de su línea materna, con un ADN recombinante capaz de expresar una proteína, caracterizada en que la proteína es la trehalosa fosfato sintetasa (TFS). Un método de acuerdo a la reivindicación 2, caracterizado en que el ADN recombinante comprende el gen codificante para la TFS siendo de origen bacteriano, fungal, animal, vegetal o humano y preferiblemente derivado de Escherichia Coli. Un método para inducir la germinación en una planta que se ha hecho no germinadora, de acuerdo al método de las reivindicaciones 2 o el 3, caracterizado en que dicha planta está dotada con ADN recombinante que codifica TFS flanqueado por sitios de blanco de una recombinasa de sitio específico y de que el ADN recombinante que codifica la TFS, es anulado mediante la dotación en dicha planta, bien sea a través de una transformación con un gen codificante para la recombinasa correspondiente, o bien sea cruzándola con una planta que albergue en su interior un ADN recombinante capaz de expresar dicha recombinasa. Un método para inducir la germinación en una planta que se ha hecho no germinadora, de acuerdo a los métodos de las reivindicaciones 2 y 3, mediante su transformación con un ADN recombinante que incluya un gen que codifique un compuesto que sea capaz de neutralizar los efectos de la TFS bajo el control de un promotor inducible y fuerce la expresión de el compuesto neutralizador TFS, mediante la inducción del promotor inducible. Un método de acuerdo a la reivindicación 5, caracterizado en que el compuesto neutralizador es la trehalosa fosfato fosfatasa (TFF).Un método de acuerdo a la reivindicación 5, caracterizado en que el compuesto neutralizador es la trehalosa fosfato sintetasa antisentido. Un método de acuerdo a la reivindicación 5, caracterizado en que el compuesto neutralizador es la trehalosa fosfato hidrolasa (Trec.).
CO98063669A 1997-10-30 1998-10-29 Inhibicion pre y post cosecha de la transformacion de pro- ductos almacenados CO4870727A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP97203371 1997-10-30

Publications (1)

Publication Number Publication Date
CO4870727A1 true CO4870727A1 (es) 1999-12-27

Family

ID=8228886

Family Applications (1)

Application Number Title Priority Date Filing Date
CO98063669A CO4870727A1 (es) 1997-10-30 1998-10-29 Inhibicion pre y post cosecha de la transformacion de pro- ductos almacenados

Country Status (17)

Country Link
US (2) US6559364B1 (es)
EP (1) EP1027449A1 (es)
JP (1) JP2001521746A (es)
CN (1) CN1280626A (es)
AR (1) AR017399A1 (es)
AU (1) AU755228B2 (es)
BR (1) BR9813340A (es)
CA (1) CA2308094A1 (es)
CO (1) CO4870727A1 (es)
EA (1) EA200000471A1 (es)
JO (1) JO2069B1 (es)
MA (1) MA24690A1 (es)
PE (1) PE131499A1 (es)
PL (1) PL341801A1 (es)
TW (1) TW517087B (es)
WO (1) WO1999023234A1 (es)
ZA (1) ZA989782B (es)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19956272B4 (de) * 1999-11-23 2004-09-16 Mpb Cologne Gmbh Molecular Plant & Protein Biotechnology Verfahren zur gesteuerten Nach-Ernte-Produktion von Proteinen in Wirtsorganismen
EP1313865A1 (en) * 2000-08-25 2003-05-28 Institute of Molecular Agrobiology Reduction of transmission of transgenes in plants
US6963797B2 (en) * 2002-08-05 2005-11-08 Ford Global Technologies, Llc System and method for determining an amount of control for operating a rollover control system
US20040163310A1 (en) * 2002-12-10 2004-08-26 Shimon Tsurgil Method of propagating bananas
IL160036A0 (en) * 2004-01-25 2004-06-20 Shimon Tsurgil Method of propagating bananas

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL103241A0 (en) 1991-10-07 1993-02-21 Univ California Tomato acid invertase gene
US5422254A (en) * 1992-02-14 1995-06-06 Oy Alko Ab Method to increase the trehalose content of organisms by transforming them with the structural genes for the short and long chains of yeast trehalose synthase
FI943133A0 (fi) 1994-06-29 1994-06-29 Alko Ab Oy Transgena vaexter
DE4213444A1 (de) * 1992-04-18 1993-10-28 Inst Genbiologische Forschung Verfahren zur Herstellung von Kartoffelpflanzen, deren Knollensprossung unterdrückt ist
EP0577915A1 (fr) 1992-07-09 1994-01-12 N.V. Algist-Bruggeman Souches de levures transformées de manière à posséder une résistance au stress et/ou un pouvoir fermentatif amélioré
WO1994028146A2 (en) * 1993-05-24 1994-12-08 Hoechst Schering Agrevo Gmbh Dna sequences and plasmids for the preparation of sugar beet with changed sucrose concentration
WO1995006126A1 (en) * 1993-08-24 1995-03-02 Mogen International N.V. Production of trehalose in plants
US20040078838A1 (en) 1994-03-09 2004-04-22 Bernd Muller-Rober Processes for inhibiting and for inducing flower formation in plants
GB9421287D0 (en) * 1994-10-21 1994-12-07 Danisco A method of reducing the level of sugar in an organism
DE4444460A1 (de) 1994-11-29 1996-05-30 Inst Genbiologische Forschung Verfahren zur Steigerung des Ertrags sowie zur Veränderung des Blühverhaltens bei Pflanzen
GB9526613D0 (en) * 1995-12-28 1996-02-28 Scottish Crop Research Inst Sequence
EP0784095A3 (en) * 1996-01-12 1997-12-29 Mogen International N.V. Enhanced accummulation of trehalose in plants
IN1997CH00924A (en) 1996-05-03 2005-03-04 Syngenta Mogen Bv Regulating metabolism by modifying the level of trehalose-6-phosphate

Also Published As

Publication number Publication date
CA2308094A1 (en) 1999-05-14
MA24690A1 (fr) 1999-07-01
EA200000471A1 (ru) 2000-10-30
US6559364B1 (en) 2003-05-06
EP1027449A1 (en) 2000-08-16
AR017399A1 (es) 2001-09-05
ZA989782B (en) 1999-05-04
TW517087B (en) 2003-01-11
BR9813340A (pt) 2000-08-22
CN1280626A (zh) 2001-01-17
US20030233678A1 (en) 2003-12-18
AU755228B2 (en) 2002-12-05
WO1999023234A1 (en) 1999-05-14
JO2069B1 (en) 2000-05-21
PE131499A1 (es) 1999-12-29
PL341801A1 (en) 2001-05-07
JP2001521746A (ja) 2001-11-13
AU1487799A (en) 1999-05-24

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