CO4870727A1 - Inhibicion pre y post cosecha de la transformacion de pro- ductos almacenados - Google Patents
Inhibicion pre y post cosecha de la transformacion de pro- ductos almacenadosInfo
- Publication number
- CO4870727A1 CO4870727A1 CO98063669A CO98063669A CO4870727A1 CO 4870727 A1 CO4870727 A1 CO 4870727A1 CO 98063669 A CO98063669 A CO 98063669A CO 98063669 A CO98063669 A CO 98063669A CO 4870727 A1 CO4870727 A1 CO 4870727A1
- Authority
- CO
- Colombia
- Prior art keywords
- plant
- tfs
- recombinant dna
- protein
- trehalose phosphate
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1048—Glycosyltransferases (2.4)
- C12N9/1051—Hexosyltransferases (2.4.1)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
- C12N15/8245—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8262—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield involving plant development
- C12N15/8267—Seed dormancy, germination or sprouting
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Nutrition Science (AREA)
- Medicinal Chemistry (AREA)
- Physiology (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Enzymes And Modification Thereof (AREA)
- Storage Of Fruits Or Vegetables (AREA)
- Pretreatment Of Seeds And Plants (AREA)
- Cultivation Of Plants (AREA)
Abstract
Un método para inhibir la removilización pre y post-cosechade compuestos almacenados en plantas mediante la transformación de una planta o de una planta obtenida a partir de su línea materna, con un ADN recombinante capaz de expresar una proteína, caracterizada en que la proteína es la trehalosa fosfato sintetasa (TFS).Un método para evitar la germinación de una parte de una planta mediante la transformación de la planta o de una planta derivada de su línea materna, con un ADN recombinante capaz de expresar una proteína, caracterizada en que la proteína es la trehalosa fosfato sintetasa (TFS). Un método de acuerdo a la reivindicación 2, caracterizado en que el ADN recombinante comprende el gen codificante para la TFS siendo de origen bacteriano, fungal, animal, vegetal o humano y preferiblemente derivado de Escherichia Coli. Un método para inducir la germinación en una planta que se ha hecho no germinadora, de acuerdo al método de las reivindicaciones 2 o el 3, caracterizado en que dicha planta está dotada con ADN recombinante que codifica TFS flanqueado por sitios de blanco de una recombinasa de sitio específico y de que el ADN recombinante que codifica la TFS, es anulado mediante la dotación en dicha planta, bien sea a través de una transformación con un gen codificante para la recombinasa correspondiente, o bien sea cruzándola con una planta que albergue en su interior un ADN recombinante capaz de expresar dicha recombinasa. Un método para inducir la germinación en una planta que se ha hecho no germinadora, de acuerdo a los métodos de las reivindicaciones 2 y 3, mediante su transformación con un ADN recombinante que incluya un gen que codifique un compuesto que sea capaz de neutralizar los efectos de la TFS bajo el control de un promotor inducible y fuerce la expresión de el compuesto neutralizador TFS, mediante la inducción del promotor inducible. Un método de acuerdo a la reivindicación 5, caracterizado en que el compuesto neutralizador es la trehalosa fosfato fosfatasa (TFF).Un método de acuerdo a la reivindicación 5, caracterizado en que el compuesto neutralizador es la trehalosa fosfato sintetasa antisentido. Un método de acuerdo a la reivindicación 5, caracterizado en que el compuesto neutralizador es la trehalosa fosfato hidrolasa (Trec.).
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP97203371 | 1997-10-30 |
Publications (1)
Publication Number | Publication Date |
---|---|
CO4870727A1 true CO4870727A1 (es) | 1999-12-27 |
Family
ID=8228886
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CO98063669A CO4870727A1 (es) | 1997-10-30 | 1998-10-29 | Inhibicion pre y post cosecha de la transformacion de pro- ductos almacenados |
Country Status (17)
Country | Link |
---|---|
US (2) | US6559364B1 (es) |
EP (1) | EP1027449A1 (es) |
JP (1) | JP2001521746A (es) |
CN (1) | CN1280626A (es) |
AR (1) | AR017399A1 (es) |
AU (1) | AU755228B2 (es) |
BR (1) | BR9813340A (es) |
CA (1) | CA2308094A1 (es) |
CO (1) | CO4870727A1 (es) |
EA (1) | EA200000471A1 (es) |
JO (1) | JO2069B1 (es) |
MA (1) | MA24690A1 (es) |
PE (1) | PE131499A1 (es) |
PL (1) | PL341801A1 (es) |
TW (1) | TW517087B (es) |
WO (1) | WO1999023234A1 (es) |
ZA (1) | ZA989782B (es) |
Families Citing this family (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE19956272B4 (de) * | 1999-11-23 | 2004-09-16 | Mpb Cologne Gmbh Molecular Plant & Protein Biotechnology | Verfahren zur gesteuerten Nach-Ernte-Produktion von Proteinen in Wirtsorganismen |
EP1313865A1 (en) * | 2000-08-25 | 2003-05-28 | Institute of Molecular Agrobiology | Reduction of transmission of transgenes in plants |
US6963797B2 (en) * | 2002-08-05 | 2005-11-08 | Ford Global Technologies, Llc | System and method for determining an amount of control for operating a rollover control system |
US20040163310A1 (en) * | 2002-12-10 | 2004-08-26 | Shimon Tsurgil | Method of propagating bananas |
IL160036A0 (en) * | 2004-01-25 | 2004-06-20 | Shimon Tsurgil | Method of propagating bananas |
Family Cites Families (13)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
IL103241A0 (en) | 1991-10-07 | 1993-02-21 | Univ California | Tomato acid invertase gene |
US5422254A (en) * | 1992-02-14 | 1995-06-06 | Oy Alko Ab | Method to increase the trehalose content of organisms by transforming them with the structural genes for the short and long chains of yeast trehalose synthase |
FI943133A0 (fi) | 1994-06-29 | 1994-06-29 | Alko Ab Oy | Transgena vaexter |
DE4213444A1 (de) * | 1992-04-18 | 1993-10-28 | Inst Genbiologische Forschung | Verfahren zur Herstellung von Kartoffelpflanzen, deren Knollensprossung unterdrückt ist |
EP0577915A1 (fr) | 1992-07-09 | 1994-01-12 | N.V. Algist-Bruggeman | Souches de levures transformées de manière à posséder une résistance au stress et/ou un pouvoir fermentatif amélioré |
WO1994028146A2 (en) * | 1993-05-24 | 1994-12-08 | Hoechst Schering Agrevo Gmbh | Dna sequences and plasmids for the preparation of sugar beet with changed sucrose concentration |
WO1995006126A1 (en) * | 1993-08-24 | 1995-03-02 | Mogen International N.V. | Production of trehalose in plants |
US20040078838A1 (en) | 1994-03-09 | 2004-04-22 | Bernd Muller-Rober | Processes for inhibiting and for inducing flower formation in plants |
GB9421287D0 (en) * | 1994-10-21 | 1994-12-07 | Danisco | A method of reducing the level of sugar in an organism |
DE4444460A1 (de) | 1994-11-29 | 1996-05-30 | Inst Genbiologische Forschung | Verfahren zur Steigerung des Ertrags sowie zur Veränderung des Blühverhaltens bei Pflanzen |
GB9526613D0 (en) * | 1995-12-28 | 1996-02-28 | Scottish Crop Research Inst | Sequence |
EP0784095A3 (en) * | 1996-01-12 | 1997-12-29 | Mogen International N.V. | Enhanced accummulation of trehalose in plants |
IN1997CH00924A (en) | 1996-05-03 | 2005-03-04 | Syngenta Mogen Bv | Regulating metabolism by modifying the level of trehalose-6-phosphate |
-
1998
- 1998-10-27 ZA ZA989782A patent/ZA989782B/xx unknown
- 1998-10-28 PE PE1998001013A patent/PE131499A1/es not_active Application Discontinuation
- 1998-10-29 AR ARP980105416A patent/AR017399A1/es not_active Application Discontinuation
- 1998-10-29 CO CO98063669A patent/CO4870727A1/es unknown
- 1998-10-29 JO JO19982069A patent/JO2069B1/en active
- 1998-10-30 EP EP98958899A patent/EP1027449A1/en not_active Withdrawn
- 1998-10-30 AU AU14877/99A patent/AU755228B2/en not_active Ceased
- 1998-10-30 BR BR9813340-3A patent/BR9813340A/pt not_active IP Right Cessation
- 1998-10-30 JP JP2000519089A patent/JP2001521746A/ja not_active Withdrawn
- 1998-10-30 CN CN98811752A patent/CN1280626A/zh active Pending
- 1998-10-30 WO PCT/EP1998/007010 patent/WO1999023234A1/en not_active Application Discontinuation
- 1998-10-30 MA MA25325A patent/MA24690A1/fr unknown
- 1998-10-30 PL PL98341801A patent/PL341801A1/xx unknown
- 1998-10-30 CA CA002308094A patent/CA2308094A1/en not_active Abandoned
- 1998-10-30 EA EA200000471A patent/EA200000471A1/ru unknown
- 1998-10-30 US US09/529,993 patent/US6559364B1/en not_active Expired - Fee Related
- 1998-11-18 TW TW087119116A patent/TW517087B/zh active
-
2002
- 2002-11-08 US US10/291,039 patent/US20030233678A1/en not_active Abandoned
Also Published As
Publication number | Publication date |
---|---|
CA2308094A1 (en) | 1999-05-14 |
MA24690A1 (fr) | 1999-07-01 |
EA200000471A1 (ru) | 2000-10-30 |
US6559364B1 (en) | 2003-05-06 |
EP1027449A1 (en) | 2000-08-16 |
AR017399A1 (es) | 2001-09-05 |
ZA989782B (en) | 1999-05-04 |
TW517087B (en) | 2003-01-11 |
BR9813340A (pt) | 2000-08-22 |
CN1280626A (zh) | 2001-01-17 |
US20030233678A1 (en) | 2003-12-18 |
AU755228B2 (en) | 2002-12-05 |
WO1999023234A1 (en) | 1999-05-14 |
JO2069B1 (en) | 2000-05-21 |
PE131499A1 (es) | 1999-12-29 |
PL341801A1 (en) | 2001-05-07 |
JP2001521746A (ja) | 2001-11-13 |
AU1487799A (en) | 1999-05-24 |
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