CO2020009638A2 - Proceso de purificación de proteína - Google Patents
Proceso de purificación de proteínaInfo
- Publication number
- CO2020009638A2 CO2020009638A2 CONC2020/0009638A CO2020009638A CO2020009638A2 CO 2020009638 A2 CO2020009638 A2 CO 2020009638A2 CO 2020009638 A CO2020009638 A CO 2020009638A CO 2020009638 A2 CO2020009638 A2 CO 2020009638A2
- Authority
- CO
- Colombia
- Prior art keywords
- protein
- purification process
- protein purification
- feed
- multimodal
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/165—Extraction; Separation; Purification by chromatography mixed-mode chromatography
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/06—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies from serum
- C07K16/065—Purification, fragmentation
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
- C07K16/24—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against cytokines, lymphokines or interferons
- C07K16/241—Tumor Necrosis Factors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/10—Separation or concentration of fermentation products
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Medicinal Chemistry (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Analytical Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Sustainable Development (AREA)
- General Engineering & Computer Science (AREA)
- Peptides Or Proteins (AREA)
Abstract
RESUMEN La presente divulgación describe un método para purificar una proteína, en donde dicha proteína está presente en una alimentación, y en donde dicho método comprende un paso de cromatografía multimodal, en donde dicha alimentación es puesta en contacto con un intercambiador iónico multimodal que comprende un ligando con un residuo hidrofóbico y un residuo cargado y en donde la unión de la proteína de interés a dicho intercambiador ocurre bajo condiciones de alta conductividad.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201862623812P | 2018-01-30 | 2018-01-30 | |
| US201862731385P | 2018-09-14 | 2018-09-14 | |
| PCT/EP2019/052104 WO2019149691A1 (en) | 2018-01-30 | 2019-01-29 | Protein purification process |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CO2020009638A2 true CO2020009638A2 (es) | 2020-12-10 |
Family
ID=65243564
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CONC2020/0009638A CO2020009638A2 (es) | 2018-01-30 | 2020-08-03 | Proceso de purificación de proteína |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US20210040146A1 (es) |
| EP (1) | EP3746538A1 (es) |
| KR (1) | KR20200131224A (es) |
| CO (1) | CO2020009638A2 (es) |
| EC (1) | ECSP20045838A (es) |
| MX (1) | MX2020008000A (es) |
| PE (1) | PE20211445A1 (es) |
| WO (1) | WO2019149691A1 (es) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AR125236A1 (es) * | 2021-03-31 | 2023-06-28 | Hoffmann La Roche | Purificación de anticuerpos por cromatografía de modo mixto |
| EP4347778A1 (en) * | 2021-05-26 | 2024-04-10 | Simabs NV | Integrated continuous bioprocess production platform |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SE9904197D0 (sv) * | 1999-11-22 | 1999-11-22 | Amersham Pharm Biotech Ab | A method for anion exchange adsorption on matrices carrying mixed mode ligands |
| US7750129B2 (en) * | 2004-02-27 | 2010-07-06 | Ge Healthcare Bio-Sciences Ab | Process for the purification of antibodies |
| WO2009126603A1 (en) | 2008-04-08 | 2009-10-15 | Bio-Rad Laboratories, Inc. | Chromatography purification of antibodies |
| EP2490780A4 (en) * | 2009-10-20 | 2014-04-09 | Merck Sharp & Dohme | USE OF A MIXED MODE CHROMATOGRAPHY FOR THE DETECTION AND PURIFICATION OF BASIC ANTIBODY PRODUCTS |
| EP2684951A1 (en) * | 2012-07-13 | 2014-01-15 | Sandoz Ag | Method for producing a recombinant protein of interest |
| WO2014179665A1 (en) * | 2013-05-03 | 2014-11-06 | Biogen Idec Inc. | Methods for increasing polypeptide recovery |
| US10927145B2 (en) * | 2016-06-15 | 2021-02-23 | Agency For Science, Technology And Research | Method of enhancing the performance of chromatography methods for purification of proteins |
| HUE068564T2 (hu) * | 2016-06-17 | 2025-01-28 | F Hoffmann La Roche Ag | Multispecifikus antitestek tisztítása |
-
2019
- 2019-01-29 MX MX2020008000A patent/MX2020008000A/es unknown
- 2019-01-29 WO PCT/EP2019/052104 patent/WO2019149691A1/en active Application Filing
- 2019-01-29 KR KR1020207024991A patent/KR20200131224A/ko unknown
- 2019-01-29 US US16/965,792 patent/US20210040146A1/en active Pending
- 2019-01-29 EP EP19702410.2A patent/EP3746538A1/en active Pending
- 2019-01-29 PE PE2020001128A patent/PE20211445A1/es unknown
-
2020
- 2020-07-31 EC ECSENADI202045838A patent/ECSP20045838A/es unknown
- 2020-08-03 CO CONC2020/0009638A patent/CO2020009638A2/es unknown
Also Published As
| Publication number | Publication date |
|---|---|
| WO2019149691A1 (en) | 2019-08-08 |
| KR20200131224A (ko) | 2020-11-23 |
| EP3746538A1 (en) | 2020-12-09 |
| ECSP20045838A (es) | 2020-08-31 |
| US20210040146A1 (en) | 2021-02-11 |
| MX2020008000A (es) | 2021-01-08 |
| PE20211445A1 (es) | 2021-08-05 |
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