AR125236A1 - Purificación de anticuerpos por cromatografía de modo mixto - Google Patents

Purificación de anticuerpos por cromatografía de modo mixto

Info

Publication number
AR125236A1
AR125236A1 ARP220100746A ARP220100746A AR125236A1 AR 125236 A1 AR125236 A1 AR 125236A1 AR P220100746 A ARP220100746 A AR P220100746A AR P220100746 A ARP220100746 A AR P220100746A AR 125236 A1 AR125236 A1 AR 125236A1
Authority
AR
Argentina
Prior art keywords
antibody
mixed mode
antibodies
purification
mode chromatography
Prior art date
Application number
ARP220100746A
Other languages
English (en)
Inventor
Roberto Falkenstein
Susanne Konrad
Original Assignee
Hoffmann La Roche
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoffmann La Roche filed Critical Hoffmann La Roche
Publication of AR125236A1 publication Critical patent/AR125236A1/es

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/395—Antibodies; Immunoglobulins; Immune serum, e.g. antilymphocytic serum
    • A61K39/39516—Antibodies; Immunoglobulins; Immune serum, e.g. antilymphocytic serum from serum, plasma
    • A61K39/39525—Purification
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/165—Extraction; Separation; Purification by chromatography mixed-mode chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/20—Partition-, reverse-phase or hydrophobic interaction chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/06—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies from serum
    • C07K16/065—Purification, fragmentation

Abstract

En el presente documento se informa un procedimiento para producir o purificar un anticuerpo usando un material de cromatografía de modo mixto que comprende grupos funcionales de intercambio iónico y grupos funcionales de interacción hidrófoba (MM HIC/IEX) operados en modo de flujo continuo, en el que el anticuerpo es un anticuerpo hidrófilo y el anticuerpo se aplica en una solución que comprende el anticuerpo y una sal anticaotrópica al material de cromatografía de MM HIC/IEX.
ARP220100746A 2021-03-31 2022-03-29 Purificación de anticuerpos por cromatografía de modo mixto AR125236A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP21166167 2021-03-31

Publications (1)

Publication Number Publication Date
AR125236A1 true AR125236A1 (es) 2023-06-28

Family

ID=75339461

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP220100746A AR125236A1 (es) 2021-03-31 2022-03-29 Purificación de anticuerpos por cromatografía de modo mixto

Country Status (11)

Country Link
EP (1) EP4314009A1 (es)
JP (1) JP2024514306A (es)
KR (1) KR20230165245A (es)
CN (1) CN117120457A (es)
AR (1) AR125236A1 (es)
AU (1) AU2022249605A1 (es)
BR (1) BR112023020173A2 (es)
CA (1) CA3214610A1 (es)
IL (1) IL307236A (es)
TW (1) TW202304946A (es)
WO (1) WO2022207615A1 (es)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116621948B (zh) * 2023-07-21 2023-10-13 易康生物(苏州)有限公司 一种重组呼吸道合胞病毒f蛋白的纯化工艺方法

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4816567A (en) 1983-04-08 1989-03-28 Genentech, Inc. Recombinant immunoglobin preparations
IE922437A1 (en) 1991-07-25 1993-01-27 Idec Pharma Corp Recombinant antibodies for human therapy
US5731168A (en) 1995-03-01 1998-03-24 Genentech, Inc. Method for making heteromultimeric polypeptides
DE69830901T2 (de) 1997-05-02 2006-05-24 Genentech Inc., San Francisco ein verfahren zur herstellung multispezifischer antikörper die heteromultimere und gemeinsame komponenten besitzen
WO1999062936A1 (en) 1998-06-01 1999-12-09 Genentech, Inc. Separation of protein monomers from aggregates by use of ion-exchange chromatography
ES2617777T5 (es) 2010-04-23 2022-10-13 Hoffmann La Roche Producción de proteínas heteromultiméricas
ES2637613T5 (es) 2010-05-25 2022-07-20 Hoffmann La Roche Procedimientos de purificación de polipéptidos
CN105324393A (zh) * 2013-05-13 2016-02-10 米迪缪尼有限公司 具有最小单体分离的重组多克隆抗体多聚体的分离
EP3676280A1 (en) * 2017-08-30 2020-07-08 Fresenius Kabi Deutschland GmbH Method for purifying anti-il-6 receptor antibodies
MX2020008000A (es) * 2018-01-30 2021-01-08 Exothera Sa Procedimiento para la purificacion de proteinas.

Also Published As

Publication number Publication date
WO2022207615A1 (en) 2022-10-06
AU2022249605A1 (en) 2023-09-14
JP2024514306A (ja) 2024-04-01
CA3214610A1 (en) 2022-10-06
TW202304946A (zh) 2023-02-01
CN117120457A (zh) 2023-11-24
EP4314009A1 (en) 2024-02-07
BR112023020173A2 (pt) 2023-11-28
IL307236A (en) 2023-11-01
KR20230165245A (ko) 2023-12-05

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