CN220335163U - Culture separation equipment for exosome - Google Patents
Culture separation equipment for exosome Download PDFInfo
- Publication number
- CN220335163U CN220335163U CN202321874542.2U CN202321874542U CN220335163U CN 220335163 U CN220335163 U CN 220335163U CN 202321874542 U CN202321874542 U CN 202321874542U CN 220335163 U CN220335163 U CN 220335163U
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- box
- separation
- filter screen
- upper side
- pipe
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- 238000000926 separation method Methods 0.000 title claims abstract description 36
- 210000001808 exosome Anatomy 0.000 title claims abstract description 26
- 238000003756 stirring Methods 0.000 claims abstract description 10
- 238000007599 discharging Methods 0.000 claims description 11
- 238000007789 sealing Methods 0.000 claims description 3
- 230000000149 penetrating effect Effects 0.000 claims description 2
- 239000007787 solid Substances 0.000 abstract description 13
- 230000000694 effects Effects 0.000 abstract description 12
- 238000001914 filtration Methods 0.000 abstract description 5
- 238000000034 method Methods 0.000 abstract description 2
- 239000011550 stock solution Substances 0.000 description 11
- 239000008280 blood Substances 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- 210000001124 body fluid Anatomy 0.000 description 2
- 239000010839 body fluid Substances 0.000 description 2
- 210000004027 cell Anatomy 0.000 description 2
- 210000004251 human milk Anatomy 0.000 description 2
- 235000020256 human milk Nutrition 0.000 description 2
- 150000002632 lipids Chemical class 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 210000002487 multivesicular body Anatomy 0.000 description 2
- 210000003296 saliva Anatomy 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 210000002700 urine Anatomy 0.000 description 2
- 102000010834 Extracellular Matrix Proteins Human genes 0.000 description 1
- 108010037362 Extracellular Matrix Proteins Proteins 0.000 description 1
- 108700011259 MicroRNAs Proteins 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 210000002744 extracellular matrix Anatomy 0.000 description 1
- 238000005194 fractionation Methods 0.000 description 1
- 230000004927 fusion Effects 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 108020004999 messenger RNA Proteins 0.000 description 1
- 239000002679 microRNA Substances 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 239000013049 sediment Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses culture separation equipment for exosomes, which comprises a treatment box and a base, wherein a motor is arranged on the upper side surface of the treatment box, the output end of the motor is connected with a connecting shaft, the bottom end of the connecting shaft penetrates through the upper side surface of the treatment box to enter the interior of the treatment box, a stirring rod is arranged on the outer side surface of the connecting shaft, a separation box is arranged on the lower side surface of the treatment box, and a shunt tube is arranged on the upper side surface of the interior of the separation box. This cultivate splitter that exosome was used is provided with shunt tubes, filter equipment, stopper, first filter screen and second filter screen, when cultivateing the separation to exosome, separate the stoste through the shunt tubes, let inside the stoste enters into two sets of filter equipment, the hierarchical filtration of rethread first filter screen and second filter screen simultaneously, let the solid separation in the stoste get rid of the effect better, and through the effect of reposition of redundant personnel, avoid appearing separating and get rid of the in-process, because the solid is too much, cause the effect that the jam influences the separation and gets rid of.
Description
Technical Field
The utility model particularly relates to the technical field of exosome separation, in particular to culture separation equipment for exosomes.
Background
Exosomes (exosomes) are membranous vesicles that are released into the extracellular matrix after fusion of intracellular multivesicular bodies (multivesicular body, MVB) with the cell membrane. Almost all types of cells can produce and release exosomes. It is a nano-scale lipid inclusion structure with the diameter of 30-100nm, and substances such as protein, mRNA, microRNA and the like are wrapped inside the nano-scale lipid inclusion structure. Exosomes naturally exist in body fluid, including blood, saliva, urine and breast milk, and secreted exosomes can enter into body fluid such as blood, saliva, urine and breast milk, reach other cells and tissues through circulatory system, produce long-range regulation and control effect, in order to improve the research efficiency to exosomes, need to carry out the separation with the exosomes.
When the existing culture separation equipment for exosomes is used, solid contents in stock solution are not directly separated and removed, so that the culture separation process is complex, and time and labor are wasted.
Disclosure of Invention
The present utility model is directed to a culture separation device for exosomes, which solves the problems set forth in the background art.
In order to achieve the above purpose, the present utility model provides the following technical solutions: the utility model provides a cultivate splitter that exosome was used, includes treatment box and base, side-mounting has the motor on the treatment box, and the motor output is connected with the connecting axle, the connecting axle bottom runs through the treatment box and goes into the inside of treatment box, and the connecting axle lateral surface is provided with the stirring rod, the treatment box downside is provided with the separator box, side-mounting has the shunt tubes on the separator box is inside, and the shunt tubes top is connected with the discharging pipe bottom, separator box inside is provided with filter equipment, and the inside left and right sides face of filter equipment is provided with the stopper, the side is provided with first filter screen and second filter screen respectively on the stopper, the filter equipment upside is connected with the shunt tubes bottom, and the filter equipment downside is provided with the pressure manifold, the separator box downside is run through to the pressure manifold bottom, and the pressure manifold leading flank is provided with the second solenoid valve.
Preferably, the upper side of the treatment box is provided with a feeding pipe, the feeding pipe is provided with two groups, and the upper side of the feeding pipe is provided with a sealing cover.
Preferably, the treatment box downside through connection has the discharging pipe, and the inside first solenoid valve that is provided with of discharging pipe, the discharging pipe bottom is connected with the separator box.
Preferably, the first filter screen and the second filter screen are respectively provided with two groups, and the first filter screen is arranged on the upper side of the second filter screen.
Preferably, the base sets up in the separator box below, and the base upside is provided with places the case, place the case setting in the pressure manifold below, and the pressure manifold bottom runs through and places the case upside and arrive and place inside the case, place inside the case and be provided with the collecting box, and the collecting box sets up in the pressure manifold below.
Compared with the prior art, the utility model has the beneficial effects that: the culture separation device for the exosome,
(1) The device is provided with the shunt tubes, the filtering devices, the limiting blocks, the first filter screen and the second filter screen, when the exosome is cultured and separated, the stock solution is separated through the shunt tubes, the stock solution enters the two groups of filtering devices, and then the stock solution is subjected to graded filtration through the first filter screen and the second filter screen, so that the solid matters in the stock solution are better in separation and removal effect, and the separation and removal effect is avoided due to the fact that the blockage influences the separation and removal effect due to the fact that the solid matters are too much in the separation and removal process;
(2) Be provided with stirring rod and collecting pipe, the stirring rod plays the stirring effect to the stoste in the processing case, avoids the solid dress thing in the stoste to deposit the condition, and the collecting pipe can concentrate the collection to the stoste after the separation is got rid of, improves collection efficiency.
Drawings
FIG. 1 is a schematic elevational view of the present utility model;
FIG. 2 is a schematic view of the front cross-section structure of the present utility model;
FIG. 3 is a schematic view of a left cut-away structure of a filter device of the present utility model;
fig. 4 is an enlarged schematic view of the structure of fig. 2 a according to the present utility model.
In the figure: 1. a treatment box; 2. a feed pipe; 3. a motor; 4. a connecting shaft; 5. a stirring rod; 6. a discharge pipe; 7. a first electromagnetic valve; 8. a separation box; 9. a shunt; 10. a filtering device; 11. a limiting block; 12. a first filter screen; 13. a second filter screen; 14. collecting pipes; 15. a second electromagnetic valve; 16. a base; 17. placing a box; 18. and (5) collecting a box.
Detailed Description
The following description of the embodiments of the present utility model will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present utility model, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.
Referring to fig. 1-4, the present utility model provides a technical solution: the utility model provides a cultivate splitter for exosome, install motor 3 on the processing box 1, and motor 3 output is connected with connecting axle 4, the connecting axle 4 bottom runs through processing box 1 up side and gets into processing box 1 inside, and the connecting axle 4 lateral surface is provided with stirring rod 5, processing box 1 downside is provided with separator box 8, side-mounting has shunt tubes 9 on the separator box 8 inside, and shunt tubes 9 top is connected with discharging pipe 6 bottom, separator box 8 inside is provided with filter equipment 10, and the inside left and right sides face of filter equipment 10 is provided with stopper 11, stopper 11 up side is provided with first filter screen 12 and second filter screen 13 respectively, filter equipment 10 up side is connected with shunt tubes 9 bottom, and filter equipment 10 downside is provided with collecting main 14, the collecting main 14 bottom runs through separator box 8 downside, and the collecting main 14 leading to side is provided with second solenoid valve 15, the solid in the stock solution is got rid of through the hierarchical separation of first filter screen 12 and second filter screen 13, make solid removal effect better, the effect of shunt tubes 9 plays the separation that the effect that the jam appears simultaneously leads to the concentrated removal condition.
As shown in fig. 1 and 2, the upper side of the treatment tank 1 is provided with a feed pipe 2, and the feed pipe 2 is provided with two groups, and the upper side of the feed pipe 2 is provided with a sealing cover, so that the stock solution throwing efficiency is not affected.
As shown in fig. 1 and 2, the lower side surface of the treatment tank 1 is connected with a discharge pipe 6 in a penetrating manner, a first electromagnetic valve 7 is arranged inside the discharge pipe 6, and the bottom end of the discharge pipe 6 is connected with a separation tank 8, so that solid matters in stock solution can be separated and removed.
As shown in fig. 2, 3 and 4, the first filter screen 12 and the second filter screen 13 are respectively provided with two groups, and the first filter screen 12 is arranged on the upper side of the second filter screen 13, so that solid matters in the stock solution can be removed by grading separation, and the solid matters in the stock solution are better in separation and removal effect.
As shown in fig. 2, the base 16 is disposed below the separation tank 8, a placement tank 17 is disposed on the upper side of the base 16, the placement tank 17 is disposed below the collecting pipe 14, the bottom end of the collecting pipe 14 penetrates through the upper side of the placement tank 17 to reach the inside of the placement tank 17, a collection tank 18 is disposed inside the placement tank 17, the collection tank 18 is disposed below the collecting pipe 14, and the separated and removed stock solution is collected intensively through the collecting pipe 14.
Working principle: when using this culture splitter that exosome was used, connect external power supply, start up motor 3, put into inside processing box 1 through inlet pipe 2 to the stoste, this moment motor 3 drives stirring rod 5 through connecting axle 4 and rotates, can stir the stoste, avoid solid sediment, open the inside first solenoid valve 7 of discharging pipe 6 this moment, make the inside stoste of processing box 1 enter into shunt tubes 9 inside through discharging pipe 6, finally enter into respectively inside two sets of filter equipment 10, get into respectively through the fractionation of first filter screen 12 and second filter screen 13 simultaneously, let the solid in the stoste get rid of, the inside of downside collecting main 14 is flowed into to the back stoste that the separation was got rid of, open the inside second solenoid valve 15 of collecting main 14 this moment, make the inside stoste of collecting main 14 get into inside collecting box 18 and collect, after the separation is got rid of, open filter equipment 10 leading flank chamber door, take out first filter screen 12 and second filter screen 13 alright clear, the content that does not describe in detail in this specification belongs to the prior art that the expert of known in the art.
Although the present utility model has been described with reference to the foregoing embodiments, it will be apparent to those skilled in the art that modifications may be made to the embodiments described, or equivalents may be substituted for elements thereof, and any modifications, equivalents, improvements and changes may be made without departing from the spirit and principles of the present utility model.
Claims (5)
1. Culture separation equipment for exosome, including processing case (1) and base (16), its characterized in that: the utility model discloses a collecting main, including processing case (1), motor (3), connecting axle (4), filter device (10), filter device (14) bottom, separating case (8) are inside to be gone up side-mounting, and motor (3) output is connected with connecting axle (4), inside processing case (1) is got into to connecting axle (4) bottom, and connecting axle (4) lateral surface is provided with stirring rod (5) on processing case (1), processing case (1) downside is provided with separator box (8), side-mounting has shunt tubes (9) on separator box (8) are inside, and shunt tubes (9) top is connected with discharging pipe (6) bottom, separator box (8) are inside to be provided with filter device (10), and filter device (10) are inside left and right sides face to be provided with stopper (11), filter device (10) are gone up the side and are connected with shunt tubes (9) bottom respectively, and filter device (10) downside is provided with separator box (14), separator box (8) downside is run through to separator box (14) bottom, and header pipe (14) front side is provided with second solenoid valve (15).
2. The culture separation device for exosomes according to claim 1, wherein: the upper side of the treatment box (1) is provided with a feeding pipe (2), the feeding pipe (2) is provided with two groups, and the upper side of the feeding pipe (2) is provided with a sealing cover.
3. The culture separation device for exosomes according to claim 1, wherein: the treatment box is characterized in that a discharging pipe (6) is connected to the lower side face of the treatment box (1) in a penetrating mode, a first electromagnetic valve (7) is arranged inside the discharging pipe (6), and a separation box (8) is connected to the bottom end of the discharging pipe (6).
4. The culture separation device for exosomes according to claim 1, wherein: the first filter screen (12) and the second filter screen (13) are respectively provided with two groups, and the first filter screen (12) is arranged on the upper side of the second filter screen (13).
5. The culture separation device for exosomes according to claim 1, wherein: the base (16) is arranged below the separation box (8), the upper side of the base (16) is provided with the placement box (17), the placement box (17) is arranged below the collecting pipe (14), the bottom end of the collecting pipe (14) penetrates through the upper side of the placement box (17) to reach the inside of the placement box (17), the collection box (18) is arranged inside the placement box (17), and the collection box (18) is arranged below the collecting pipe (14).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321874542.2U CN220335163U (en) | 2023-07-17 | 2023-07-17 | Culture separation equipment for exosome |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321874542.2U CN220335163U (en) | 2023-07-17 | 2023-07-17 | Culture separation equipment for exosome |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN220335163U true CN220335163U (en) | 2024-01-12 |
Family
ID=89456042
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202321874542.2U Active CN220335163U (en) | 2023-07-17 | 2023-07-17 | Culture separation equipment for exosome |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN220335163U (en) |
-
2023
- 2023-07-17 CN CN202321874542.2U patent/CN220335163U/en active Active
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