CN213977499U - Antibody extraction equipment - Google Patents
Antibody extraction equipment Download PDFInfo
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- CN213977499U CN213977499U CN202022421435.7U CN202022421435U CN213977499U CN 213977499 U CN213977499 U CN 213977499U CN 202022421435 U CN202022421435 U CN 202022421435U CN 213977499 U CN213977499 U CN 213977499U
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- bottom plate
- antibody
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- pipe
- crushing
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- 238000000605 extraction Methods 0.000 title claims abstract description 36
- 239000007788 liquid Substances 0.000 claims abstract description 71
- 239000012530 fluid Substances 0.000 claims abstract description 8
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- 238000003756 stirring Methods 0.000 claims description 21
- 239000007787 solid Substances 0.000 claims description 14
- 238000009826 distribution Methods 0.000 claims description 12
- 238000003825 pressing Methods 0.000 claims description 12
- 238000000926 separation method Methods 0.000 claims description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 9
- 230000007246 mechanism Effects 0.000 claims description 7
- 230000005540 biological transmission Effects 0.000 claims description 5
- 239000002245 particle Substances 0.000 claims description 5
- 238000013019 agitation Methods 0.000 claims description 3
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- 108090000623 proteins and genes Proteins 0.000 description 4
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Images
Abstract
The utility model discloses an antibody extraction device, which mainly aims to solve the problems that the traditional antibody extraction device is too complex, different instruments are needed to finish different extraction steps, and the extraction speed is slow; this antibody extraction equipment includes the bottom plate, the bottom plate upper end is provided with two stands, stand fixedly connected with workstation, the workstation upper surface sets up crushing case, fixed surface is provided with the fixed block on the bottom plate, be provided with the agitator tank on the fixed block, the agitator tank bottom is provided with the fluid-discharge tube, the bottom plate upper surface is provided with the support, be provided with branch fluid reservoir on the support, branch fluid reservoir bottom is provided with the drain pipe, the drain pipe lower extreme is provided with divides liquid switch, be provided with the stock solution bottle under the liquid outlet. The antibody extraction equipment adopts integrated automatic operation, does not need to transfer the intermediate product artificially, has high extraction rate and low cost, and is suitable for industrial production.
Description
Technical Field
The utility model relates to an antibody extraction equipment field specifically is an antibody extraction equipment.
Background
The antibody is a protein having a protective effect produced by the body due to stimulation by an antigen, which is a large Y-shaped protein secreted by plasma cells and used by the immune system to identify and neutralize foreign substances such as bacteria or viruses, and is found only in body fluids such as blood of vertebrates, and on the cell membrane surface of B cells thereof. The antibody is used as reagent for in vitro diagnosis, medicine for treating diseases, ligand for immunoaffinity chromatography, etc.
When an antibody is used for carrying out a corresponding experiment, the antibody with high specificity and high titer needs to be prepared, in the prior art, although an extraction device for extracting antibody protein can extract the antibody, the extraction device has a complex structure during extraction, different steps of extraction can be completed only by using different instruments, the extraction speed is slow, and the efficiency of antibody production is reduced; the existing extraction equipment has low practicability and the defect of incomplete antibody extraction in use, thereby slowing down the progress of antibody extraction, wasting a large amount of time of a user, increasing the labor capacity of the user and being inconvenient for the user to use.
SUMMERY OF THE UTILITY MODEL
An object of the utility model is to provide an antibody extraction equipment to reach above-mentioned purpose.
In order to achieve the above object, the utility model provides a following technical scheme:
an antibody extraction device comprises a bottom plate, wherein two upright columns are arranged at the upper end of the bottom plate, a workbench is transversely and fixedly connected with the upper ends of the two upright columns, a crushing box with a square structure is arranged at the center of the upper surface of the workbench, two symmetrical crushing devices for crushing solids are arranged on the side wall of the crushing box, each crushing device comprises an air cylinder, a pressing block and crushing teeth arranged on the pressing block, the air cylinders are fixedly arranged on the upper surface of the workbench, the pressing block penetrates through the side wall of the crushing box and is fixedly connected with the telescopic end of the air cylinder, a fixed block is fixedly arranged on the upper surface of the bottom plate, an agitating tank is arranged on the fixed block, a rotating rod penetrating through the bottom of the agitating tank is vertically arranged at the top of the fixed block, the rotating rod is rotatably connected with a bearing arranged on the fixed block, agitating blades are fixedly arranged at the top of the rotating rod, and a driving mechanism for driving the rotating rod to rotate is arranged on the bottom plate, the agitator tank bottom is provided with the fluid-discharge tube, the fluid-discharge tube other end is provided with the water pump, the water pump is provided with the feed liquor pipe, the bottom plate upper surface is provided with the support, be provided with branch fluid reservoir on the support, divide fluid reservoir bottom to be provided with the drain pipe, the drain pipe lower extreme is provided with divides the liquid switch, divide the liquid switch to include a support frame, a rotary switch and two branch liquid pipes, rotary switch is the cylinder groove normal running fit of cylinder structure and support frame inside, the inside L shape that is provided with of rotary switch crosses liquid pipe, two divide liquid pipe symmetric distribution in support frame both sides, divide liquid pipe mouth of pipe below to be provided with the stock solution bottle.
On the basis of the technical scheme, the utility model discloses still provide following optional technical scheme:
in one alternative: the stirring tank top sets up the charge door of infundibulate, charge door inside is provided with the filter screen.
In one alternative: the bottom of the crushing box is funnel-shaped, and the bottom of the crushing box and the top of the stirring tank are both provided with a strainer for blocking solid particles.
In one alternative: and a heating wire is arranged in the interlayer on the side wall of the liquid separation tank.
In one alternative: the drive mechanism comprises a rotating motor, a transmission belt and a belt pulley arranged on the rotating rod, and the rotating motor is connected with the belt pulley through the transmission belt.
Compared with the prior art, the beneficial effects of the utility model are as follows:
1. the antibody extraction equipment adopts integrated operation to automatically complete, does not need to artificially transfer each intermediate product, automatically stratifies the extracting solution after mixing and stirring, is beneficial to separating subsequent antibodies from other impurities, reduces the labor intensity, does not need to pass through a complex process, is easy to operate, has high extraction rate and low cost, and is suitable for industrial production
2. The inside stirring vane that is provided with of agitator tank of antibody extraction equipment prevents that the extract stirring is insufficient, has guaranteed the more abundant mixture of extract and additive, has saved the step of user's manual stirring for the progress that the antibody drawed, setting through the bottom fixed block can play reinforced (rfd) effect to the agitator tank, the situation that rocks appears when having avoided the agitator tank to use, stability when having improved the stirring and mixing extract, made things convenient for user's use.
3. The antibody extraction equipment is provided with a leakage net and a filter screen, so that impurities in the extract can be filtered, and the purity of the antibody in the extracting solution is ensured; the strainer and the filter screen can be repeatedly cleaned and used, the economy is high, and the service life is long.
Drawings
FIG. 1 is a schematic view of the structure of an antibody extraction apparatus.
FIG. 2 is a schematic diagram of a liquid separating switch in the antibody extraction apparatus.
FIG. 3 is a schematic view of the structure of an agitation mechanism in the antibody extraction apparatus.
Notations for reference numerals: 1-bottom plate, 2-upright post, 3-workbench, 4-cylinder, 5-briquetting, 6-crushing box, 7-leakage net, 8-stirring tank, 9-rotating motor, 10-driving belt, 11-rotating rod, 12-stirring blade, 13-liquid discharge pipe, 14-water pump, 15-liquid inlet pipe, 16-bracket, 17-liquid separation tank, 18-liquid discharge pipe, 19-liquid storage bottle, 20-liquid separation switch, 201-support frame, 202-rotating switch, 203-liquid separation pipe, 21-charging hole, 22-filter screen, 23-fixed block, 24-belt pulley, 25-heating wire.
Detailed Description
The present invention will be described in detail with reference to the following embodiments, wherein like or similar elements are designated by like reference numerals throughout the drawings or description, and wherein the shape, thickness or height of the various elements may be expanded or reduced in practical applications. The embodiments of the present invention are provided only for illustration, and not for limiting the scope of the present invention. Any obvious and obvious modifications or alterations to the present invention can be made without departing from the spirit and scope of the present invention.
Referring to fig. 1-3, in the embodiment of the present invention, an antibody extraction apparatus comprises a bottom plate 1, two columns 2 are disposed on the upper end of the bottom plate 1, a worktable 3 is transversely and fixedly connected to the upper ends of the two columns 2, a square-structured crushing box 6 is disposed at the center of the upper surface of the worktable 3, two symmetrical crushing devices for crushing solid are disposed on the side wall of the crushing box 6, each crushing device comprises a cylinder 4, a pressing block 5 and crushing teeth 26 disposed on the pressing block 5, the cylinder 4 is fixedly disposed on the upper surface of the worktable 3, the pressing block 5 penetrates through the side wall of the crushing box 6 and is fixedly connected to the telescopic end of the cylinder 4, the cylinder 4 drives the pressing block 5 to slide on the upper surface of the worktable 3 through the telescopic end, a fixed block 23 is fixedly disposed on the upper surface of the bottom plate 1, an agitator tank 8 is disposed on the fixed block 23, a rotating rod 11 passing through the bottom of the agitator tank 8 is vertically disposed on the top of the fixed block 23, dwang 11 and the bearing that sets up on fixed block 23 rotate and are connected, the fixed stirring vane 12 that is provided with in dwang 11 top, dwang 11 drives stirring vane 12 and rotates 8 inside agitator tanks, be provided with the actuating mechanism that drives dwang 11 pivoted on the bottom plate 1.
The utility model discloses a stirring tank, including agitator tank 8, bottom, water pump 14, bottom plate, liquid distribution switch 20, liquid distribution switch 202, liquid distribution pipe 203, liquid storage bottle 19, liquid storage tube 203, liquid pump 13 other end, water pump 14 is provided with feed liquor pipe 15, the bottom plate upper surface is provided with support 16, be provided with on support 16 and divide liquid jar 17, water pump 14 takes out the inside liquid of agitator tank 8 through liquid discharge pipe 13 and pours into and divide liquid jar 17, it is provided with drain pipe 18 to divide liquid jar 17 bottom, the drain pipe 18 lower extreme is provided with liquid distribution switch 20, liquid distribution switch 20 includes a support frame 201, a rotary switch 202 and two liquid distribution pipes 203, rotary switch 202 is the inside cylinder groove normal running fit of cylinder structure and support frame 201, the inside L shape of being provided with liquid distribution pipe, two liquid distribution pipe 203 symmetric distribution pipe mouth below is provided with liquid storage bottle 19.
8 tops of agitator tank set up the charge door 21 of infundibulate, 21 inside filter screen 22 that is provided with of charge door, filter screen 22 can filter the liquid of adding, smash 6 bottoms of case for the infundibulate, smash 6 bottoms of case and all be provided with strainer 7 with 8 tops of agitator tank, strainer 7 separates the solid particle who produces in smashing case 6, be provided with heater strip 25 in the branch fluid reservoir 17 lateral wall intermediate layer, the heater strip can guarantee to divide the inside constancy of temperature of fluid reservoir 17, actuating mechanism is including rotating the belt pulley 24 that sets up on motor 9, drive belt 10 and the dwang 11, it is connected with belt pulley 24 through drive belt 10 to rotate motor 9, drive belt 10 drives dwang 11 through drive belt 10 and rotates.
The utility model discloses a theory of operation is: when the device is used, protein solid or tissue blocks to be extracted are placed into the crushing box 6, the air cylinder 4 is opened, the air cylinder 4 drives the pressing block 5 to gradually approach to the center of the crushing box 6, the side wall of the pressing block 5 is provided with crushing teeth 26 for crushing the solid, the two symmetrical crushing teeth 26 are meshed with each other to crush the solid into solid particles, the crushing teeth 26 are driven by the expansion and contraction of the air cylinder 4 to continuously crush the solid, the bottom of the crushing box 6 is provided with a leakage net, and the solid particles enter the stirring tank 8 through the leakage net; after the solid is crushed, adding a liquid catalyst and other substances required for extraction through a feeding port 21 arranged at the top of the stirring tank 8, after the charging is finished, opening a rotating motor 9, driving a rotating rod 11 to rotate through a transmission belt 10 by the rotating motor 9, driving a stirring blade 12 to rotate by the rotating rod 11, stirring the mixed liquid in the stirring tank 8 by the stirring blade 12, after the solid is completely dissolved, closing the rotating motor 9, opening a valve of a liquid discharge pipe 13, pumping the liquid in the stirring tank 8 out through the liquid discharge pipe 13 by a water pump 14 and injecting the liquid into a liquid separation tank 17 through a liquid inlet pipe 15, adjusting the temperature of the liquid separation tank 17 to the temperature required for liquid separation and standing, after the mixed liquid in the liquid separation tank 17 is layered, rotating a rotating switch 202 on a liquid separation switch 20, connecting the liquid outlet pipe 18 with a liquid separation pipe 203 through a liquid passing pipe in the rotating switch 202, and enabling the liquid at the bottom layer of the liquid separation tank 17 to flow into a liquid storage bottle 19, after the bottom layer liquid flows out, the rotary switch 202 is rotated, the liquid outlet pipe 18 is communicated with the other liquid distribution pipe 203 through the liquid passing pipe, and the upper layer liquid flows into other liquid storage bottles; and (3) successively putting the liquid in different layers in the liquid separating tank 17 into different liquid storage bottles, and finishing the antibody extraction operation after the liquid in the liquid separating tank 17 completely flows out.
The above description is only for the specific embodiments of the present disclosure, but the scope of the present disclosure is not limited thereto, and any person skilled in the art can easily conceive of the changes or substitutions within the technical scope of the present disclosure, and all the changes or substitutions should be covered within the scope of the present disclosure. Therefore, the protection scope of the present disclosure shall be subject to the protection scope of the claims.
Claims (5)
1. The antibody extraction equipment comprises a bottom plate (1) and is characterized in that two upright columns (2) are arranged at the upper end of the bottom plate (1), two working tables (3) are transversely and fixedly connected to the upper ends of the upright columns (2), a crushing box (6) of a square structure is arranged at the center of the upper surface of each working table (3), two symmetrical crushing devices for crushing solids are arranged on the side wall of each crushing box (6), each crushing device comprises a cylinder (4), a pressing block (5) and crushing teeth (26) arranged on the pressing block (5), the cylinder (4) is fixedly arranged on the upper surface of each working table (3), the pressing block (5) penetrates through the side wall of each crushing box (6) and is fixedly connected with the telescopic end of the cylinder (4), a fixing block (23) is fixedly arranged on the upper surface of the bottom plate (1), and a stirring tank (8) is arranged on the fixing block (23), fixed block (23) top is provided with dwang (11) that passes agitator tank (8) bottom perpendicularly, the bearing that sets up on dwang (11) and fixed block (23) rotates to be connected, dwang (11) top is fixed to be provided with stirring vane (12), be provided with drive dwang (11) pivoted actuating mechanism on bottom plate (1), agitator tank (8) bottom is provided with fluid-discharge tube (13), fluid-discharge tube (13) other end is provided with water pump (14), water pump (14) are provided with feed liquor pipe (15), the bottom plate upper surface is provided with support (16), be provided with branch fluid reservoir (17) on support (16), branch fluid reservoir (17) bottom is provided with drain pipe (18), drain pipe (18) lower extreme is provided with branch liquid switch (20), divide liquid switch (20) to include one support frame (201), One rotates switch (202) and two and divides liquid pipe (203), rotate switch (202) and be cylinder structure and the inside cylinder groove normal running fit of support frame (201), rotate switch (202) inside and be provided with L shape liquid pipe, two divide liquid pipe (203) symmetric distribution in support frame (201) both sides, divide liquid pipe (203) mouth of pipe below to be provided with stock solution bottle (19).
2. The antibody extraction apparatus according to claim 1, wherein a funnel-shaped feed opening (21) is provided at the top of the agitation tank (8), and a strainer (22) is provided inside the feed opening (21).
3. The antibody extraction apparatus according to claim 1, wherein the bottom of the pulverization chamber (6) is funnel-shaped, and the bottom of the pulverization chamber (6) and the top of the agitation tank (8) are provided with a strainer (7) for blocking solid particles.
4. The antibody extraction apparatus according to claim 1, wherein a heating wire (25) is provided in an interlayer of a side wall of the separation tank (17).
5. The antibody extraction apparatus according to claim 1, wherein the drive mechanism comprises a rotating motor (9), a transmission belt (10), and a belt pulley (24) provided on the rotating lever (11), and the rotating motor (9) is connected to the belt pulley (24) through the transmission belt (10).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202022421435.7U CN213977499U (en) | 2020-10-27 | 2020-10-27 | Antibody extraction equipment |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202022421435.7U CN213977499U (en) | 2020-10-27 | 2020-10-27 | Antibody extraction equipment |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN213977499U true CN213977499U (en) | 2021-08-17 |
Family
ID=77258934
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202022421435.7U Active CN213977499U (en) | 2020-10-27 | 2020-10-27 | Antibody extraction equipment |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN213977499U (en) |
-
2020
- 2020-10-27 CN CN202022421435.7U patent/CN213977499U/en active Active
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20240205 Address after: No. 26 Development Zone Road, Economic and Technological Development Zone, Fengning Manchu Autonomous County, Chengde City, Hebei Province, 068350 Patentee after: Fengning Manchu Autonomous County Zhongcheng Pharmaceutical Technology Co.,Ltd. Country or region after: China Address before: 1-2302-7, 15612 Century Avenue, high tech Zone, Jinan City, Shandong Province Patentee before: Shandong Saiya Biotechnology Co.,Ltd. Country or region before: China |
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| TR01 | Transfer of patent right |