CN220766993U - Cell microvesicle collection device - Google Patents
Cell microvesicle collection device Download PDFInfo
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- CN220766993U CN220766993U CN202322458895.0U CN202322458895U CN220766993U CN 220766993 U CN220766993 U CN 220766993U CN 202322458895 U CN202322458895 U CN 202322458895U CN 220766993 U CN220766993 U CN 220766993U
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- filter
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- tank body
- apoptotic bodies
- outer layer
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- 239000012528 membrane Substances 0.000 claims abstract description 61
- 230000001640 apoptogenic effect Effects 0.000 claims abstract description 44
- 239000007788 liquid Substances 0.000 claims abstract description 38
- 238000001914 filtration Methods 0.000 claims abstract description 25
- 230000006907 apoptotic process Effects 0.000 claims abstract description 22
- 238000003756 stirring Methods 0.000 claims abstract description 19
- 238000011419 induction treatment Methods 0.000 claims abstract description 5
- 230000002572 peristaltic effect Effects 0.000 claims description 11
- 239000000706 filtrate Substances 0.000 claims description 3
- 238000000034 method Methods 0.000 abstract description 9
- 230000008569 process Effects 0.000 abstract description 7
- 238000004519 manufacturing process Methods 0.000 abstract description 3
- 210000004027 cell Anatomy 0.000 description 25
- 239000000644 isotonic solution Substances 0.000 description 9
- 239000000243 solution Substances 0.000 description 7
- 239000000047 product Substances 0.000 description 3
- 239000000725 suspension Substances 0.000 description 3
- 230000009471 action Effects 0.000 description 2
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- 239000006285 cell suspension Substances 0.000 description 2
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- 210000001808 exosome Anatomy 0.000 description 2
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- 208000027418 Wounds and injury Diseases 0.000 description 1
- 238000007792 addition Methods 0.000 description 1
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- 238000009776 industrial production Methods 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 210000000633 nuclear envelope Anatomy 0.000 description 1
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- 239000006228 supernatant Substances 0.000 description 1
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Landscapes
- Separation Using Semi-Permeable Membranes (AREA)
Abstract
The utility model provides a cell microvesicle collecting device, which comprises an inner layer membrane bag, an outer layer tank body, a stirring device and a filtering device; the inner layer membrane bag is arranged in the outer layer tank body, the lower end of the outer layer tank body is provided with a liquid outlet, the liquid outlet is communicated with the inside of the filter device through a pipeline, and a filter membrane is arranged in the filter device; the stirring device is arranged in the outer tank body and is used for stirring the liquid in the outer tank body; the inner membrane bag is a semipermeable membrane and is used for filtering out apoptotic bodies continuously produced by cells after apoptosis induction treatment; the filter membrane is used for separating the apoptotic bodies from the solution, so that the apoptotic bodies are collected in the upper space of the filter membrane in the filter device. The cell microvesicle collecting device provided by the utility model can be used for rapidly collecting apoptotic bodies generated in the apoptosis process, and synchronously concentrating, so that the internal loss of the apoptotic bodies in the apoptosis process is reduced, more apoptotic bodies are ensured to be obtained from the same number of cells, and the production efficiency is improved.
Description
Technical Field
The utility model relates to the technical field of bioengineering, in particular to a cell microvesicle collecting device.
Background
After the cells receive the apoptosis signals, an apoptosis program is started, the cell volume is reduced, the nuclear mass is concentrated, the nuclear membrane nucleolus is broken, the cell membrane structure is still complete but a small bubble structure is formed on the membrane, and finally the cells are divided and wrapped into a plurality of apoptosis bodies, wherein the apoptosis bodies can be rapidly phagocytized by adjacent cells, and substances are reused after being digested.
Because apoptosis is a physiological process in a unit of single cell, even though apoptosis induction is uniformly performed, the process is asynchronous, and the whole process can be as long as tens of hours, which means that apoptotic bodies generated by cells undergoing apoptosis earlier can be phagocytized by cells not undergoing apoptosis yet, and the contents of the apoptotic bodies can be digested and decomposed into basic cell nutrients, so that the quantity of the apoptotic bodies finally obtained is greatly reduced.
The current method for collecting apoptotic bodies comprises inducing cells with inducer to start apoptosis process, and placing at 37deg.C and 5% CO 2 And (3) continuously incubating for ten or more hours in a saturated humidity incubator, namely in a cell culture environment, collecting the supernatant, and centrifugally collecting apoptotic bodies at a high speed of 4 ℃ for subsequent research and application. With the apoptosis of cells, apoptotic bodies are continuously generated, wherein the apoptotic bodies generated first are phagocytized and degraded by cells which do not undergo apoptosis if not collected in time, so that the whole yield is reduced, and the problem is not solved at present.
Apoptotic bodies are a current research hot spot, especially those of stem cells, have application range and therapeutic effect close to those of exosomes, have wide application prospect in the direction of injury repair, and compared with exosomes, the single availability of equal cells is large, and along with the vigorous development of the biomedical field, the development of the products is believed to be increased in the next few years. Therefore, preparation for industrialization of apoptotic body drug production is needed, and how to collect apoptotic bodies efficiently is a problem to be solved.
Disclosure of Invention
Aiming at the problems existing in the prior art, the utility model provides the cell microvesicle collecting device which can collect apoptotic bodies in time and increase the yield of the apoptotic bodies per unit cell quantity, thereby laying a foundation for the industrial production of related products.
The utility model is realized by the following technical scheme:
providing a cell microvesicle collecting device, comprising an inner layer membrane bag, an outer layer tank body, a stirring device and a filtering device;
the inner layer membrane bag is arranged in the outer layer tank body, a liquid outlet is formed in the lower end of the outer layer tank body, the liquid outlet is communicated with the inside of the filter device through a pipeline, and a filter membrane is arranged in the filter device; the stirring device is arranged in the outer layer tank body and is used for stirring liquid in the outer layer tank body;
the inner membrane bag is a semipermeable membrane and is used for filtering out apoptotic bodies continuously generated by cells after apoptosis induction treatment; the filter membrane is used for separating the apoptotic bodies from the solution, so that the apoptotic bodies are collected in the upper space of the filter membrane in the filter device.
As a further explanation of the present utility model, the collecting device further comprises a return pipe and a peristaltic pump, wherein one end of the return pipe is connected to a liquid outlet of the filtering device, the other end of the return pipe is connected to a return pipe interface of the outer tank, the liquid outlet is located below the filter membrane, and the peristaltic pump is used for feeding the filtrate flowing into the return pipe into the outer tank.
As a further illustration of the utility model, the peristaltic pump is snapped onto the return tube.
As a further explanation of the utility model, two ends of the return pipe are detachably connected with the return pipe interface and the liquid discharge port respectively.
As a further explanation of the utility model, the upper end of the outer layer tank body is provided with a mounting opening, the inner layer film bag is fixedly connected in the mounting opening, and the upper end of the inner layer film bag is provided with a liquid inlet.
As a further explanation of the utility model, the upper end of the inner layer film bag is also provided with an air hole.
As a further illustration of the utility model, the filter device side wall is provided with a collection port located at the upper end of the filter membrane.
As a further illustration of the utility model, the filter membrane is arranged obliquely, and the collecting port is positioned at the upper end of the lowest point of the filter membrane.
As a further explanation of the present utility model, a filter frame is attached to the inner wall of the filter device, and the filter membrane is mounted on the filter frame.
Compared with the prior art, the utility model has the following beneficial technical effects:
the cell microvesicle collecting device provided by the utility model can be used for rapidly collecting apoptotic bodies generated in the apoptosis process, so that the internal loss of the apoptotic bodies in the apoptosis process is reduced, more apoptotic bodies are ensured to be obtained from the same number of cells, and the production efficiency is improved.
The apoptotic body solution collected by the method is synchronously concentrated to obtain the apoptotic body suspension with high concentration and direct application.
Drawings
Fig. 1 is a schematic diagram of the overall structure of the cell microvesicle collection device according to the present utility model.
Reference numerals:
1. the device comprises an air hole, a liquid inlet, an inner layer membrane bag, an outer layer tank, a stirring device, a liquid outlet, a filtering device, a filtering membrane, a liquid outlet, a backflow pipe, a peristaltic pump, a backflow pipe interface, a mounting port and a collecting port.
Description of the embodiments
For the purpose of making the objects, technical solutions and advantages of the embodiments of the present utility model more apparent, the technical solutions of the embodiments of the present utility model will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present utility model, and it is apparent that the described embodiments are some embodiments of the present utility model, but not all embodiments of the present utility model. The components of the embodiments of the present utility model generally described and illustrated in the figures herein may be arranged and designed in a wide variety of different configurations.
Thus, the following detailed description of the embodiments of the utility model, as presented in the figures, is not intended to limit the scope of the utility model, as claimed, but is merely representative of selected embodiments of the utility model. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.
It should be noted that: like reference numerals and letters denote like items in the following figures, and thus once an item is defined in one figure, no further definition or explanation thereof is necessary in the following figures.
In the description of the present utility model, it should be noted that, directions or positional relationships indicated by terms such as "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc., are directions or positional relationships based on those shown in the drawings, or are directions or positional relationships conventionally put in use of the inventive product, are merely for convenience of describing the present utility model and simplifying the description, and are not indicative or implying that the apparatus or element to be referred to must have a specific direction, be constructed and operated in a specific direction, and thus should not be construed as limiting the present utility model. Furthermore, the terms "first," "second," "third," and the like are used merely to distinguish between descriptions and should not be construed as indicating or implying relative importance.
Furthermore, the terms "horizontal," "vertical," and the like do not denote a requirement that the component be absolutely horizontal or overhang, but rather may be slightly inclined. As "horizontal" merely means that its direction is more horizontal than "vertical", and does not mean that the structure must be perfectly horizontal, but may be slightly inclined.
In the description of the present utility model, it should also be noted that, unless explicitly specified and limited otherwise, the terms "disposed," "mounted," "connected," and "connected" are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium, and can be communication between two elements. The specific meaning of the above terms in the present utility model will be understood in specific cases by those of ordinary skill in the art.
The technical scheme of the present utility model will be explained in conjunction with specific embodiments.
As shown in fig. 1, the present application provides a cell microvesicle collecting device, which comprises an inner layer membrane bag 3, an outer layer tank 4, a stirring device 5 and a filtering device 7; the inner layer membrane bag 3 is arranged inside the outer layer tank body 4, a liquid outlet 6 is arranged at the lower end of the outer layer tank body 4, the liquid outlet 6 is communicated with the inside of the filter device 7 through a pipeline, and a filter membrane 8 is arranged in the filter device 7; the stirring device 5 is arranged in the outer tank body 4 and is used for stirring the liquid in the outer tank body 4; the inner layer membrane bag 3 is a semipermeable membrane and is used for filtering out apoptotic bodies continuously produced by cells after apoptosis induction treatment; the filter membrane 8 is used to separate the apoptotic bodies from the solution, so that the apoptotic bodies are collected in the filter device 7 in the upper space of the filter membrane 8.
When the collecting device is used, normal isotonic solution such as normal saline or PBS and the like in the cell culture process is firstly added into the inner layer membrane bag 3, and the isotonic solution flows into the outer layer tank body 4 through the inner layer membrane bag 3 (the aperture is less than or equal to 5 microns, the isotonic solution freely passes through, the volume of apoptotic bodies is small and can not pass through, and the cell volume is large); then adding cell suspension or cell-microcarrier suspension subjected to apoptosis induction treatment into the inner membrane bag 3, starting the bottom stirring device 5, enabling the apoptotic bodies generated after apoptosis of cells in the inner membrane bag 3 to pass through the membrane bag along with the liquid flowing, distributing the apoptotic bodies in isotonic liquid of the outer tank body, enabling the isotonic liquid containing the apoptotic bodies to enter the filtering device 7 through the liquid outlet 6, enabling the isotonic liquid to pass through the filtering membrane 8 (aperture is smaller than 0.5 micron, enabling the isotonic solution to pass through freely), concentrating the apoptotic bodies (diameter is 1-5 microns), collecting the liquid in the upper space of the filtering membrane 8 in the filtering device 7 for a period of time, and finally taking out the liquid, namely the apoptotic body concentrated liquid.
In some embodiments, the stirring device 5 may be implemented by using a motor to drive a stirring paddle, wherein the motor is assembled below the outer tank 4, and an output shaft of the motor is connected to the stirring paddle, and the stirring paddle is located at a position below the inner part of the outer tank 4, so as to stir the liquid.
In some embodiments, the collecting device further comprises a return pipe 10 and a peristaltic pump 11, wherein one end of the return pipe 10 is connected to the liquid outlet 9 of the filtering device 7, the other end of the return pipe is connected to the return pipe interface 12 of the outer layer tank 4, the liquid outlet 9 is located below the filter membrane 8, the peristaltic pump 11 is used for delivering the filtrate flowing into the return pipe 10 into the outer layer tank 4, and the peristaltic pump 11 is clamped on the return pipe 10.
Under the suction action of the peristaltic pump 11, isotonic solution containing apoptotic bodies can smoothly enter the filtering device 7 through the liquid outlet 6, and can more quickly pass through the filter membrane 8 (the aperture is smaller than 0.5 microns, the isotonic solution freely passes through) under the suction action, the apoptotic bodies (the diameter is 1-5 microns) are concentrated, the discharged isotonic solution is returned to the outer layer tank 4 through the return pipe 10 for recycling, and after the isotonic solution is continuously collected for a period of time, the liquid in the upper space of the filter membrane 8 in the filtering device 7 is finally taken out, namely the apoptotic body concentrated solution.
In some embodiments, both ends of the return tube 10 are detachably connected to the return tube connection 12 and the drain port 9, respectively, and if the concentration of the apoptotic body concentrate is to be further increased, the final volume is reduced, the connection between the return tube 10 and the outer tank 4 may be disconnected at the return tube connection 12 at a later stage of collection.
In some embodiments, the upper end of the outer layer tank body 4 is provided with the mounting opening 13, the inner layer membrane bag 3 is fixedly connected in the mounting opening 13, the upper end of the inner layer membrane bag 3 is provided with the liquid inlet 2, and the arrangement can more conveniently add isotonic solution, cell suspension, cell-microcarrier suspension and the like into the inner layer membrane bag 3. Further, the upper end of the inner layer film bag 3 is also provided with an air hole 1, and the arrangement of the air hole 1 can ensure that the air pressure of the inner layer film bag 3 is normal.
In some embodiments, the side wall of the filtering device 7 is provided with a collecting port 14, the collecting port 14 is positioned at the upper end of the filtering membrane 8, and the collecting port 14 is arranged to be convenient for collecting the apoptotic body concentrated solution. In other embodiments, the filtering device 7 and the liquid outlet 6 can be detachably connected, so that the apoptotic body concentrated liquid in the filtering device 7 can be conveniently poured out from the upper port of the filtering device.
In some embodiments, the filter membrane 8 is disposed obliquely, and the collection port 14 is located at the upper end of the lowest point of the filter membrane 8; the filter device 7 inner wall is connected with the filter frame, assembles filter membrane 8 on the filter frame, and the cooperation of filter membrane 8 and filter frame of this department can adopt current filter screen mounting structure, installs whole filtration, with its fixed mounting at filter device 7 inner wall can, the slope setting of filter membrane 8 and the setting of collecting port 14 position can be convenient for fully collect the apoptosis body concentrate in the filter device 7.
The embodiments given above are preferred examples for realizing the present utility model, and the present utility model is not limited to the above-described embodiments. Any immaterial additions and substitutions made by those skilled in the art according to the technical features of the technical scheme of the utility model are all within the protection scope of the utility model.
Claims (9)
1. The cell microvesicle collecting device is characterized by comprising an inner layer membrane bag (3), an outer layer tank body (4), a stirring device (5) and a filtering device (7);
the inner layer membrane bag (3) is arranged inside the outer layer tank body (4), a liquid outlet (6) is formed in the lower end of the outer layer tank body (4), the liquid outlet (6) is communicated with the filter device (7) through a pipeline, and a filter membrane (8) is arranged in the filter device (7); the stirring device (5) is arranged in the outer layer tank body (4) and is used for stirring liquid in the outer layer tank body (4);
the inner layer membrane bag (3) is a semipermeable membrane and is used for filtering out apoptotic bodies continuously produced by cells after apoptosis induction treatment; the filter membrane (8) is used for separating the apoptotic bodies from the solution, so that the apoptotic bodies are collected in the upper space of the filter membrane (8) in the filter device (7).
2. The cell microvesicle collection device according to claim 1, further comprising a return tube (10) and a peristaltic pump (11), wherein one end of the return tube (10) is connected to a liquid outlet (9) of the filter device (7), and the other end is connected to a return tube interface (12) of the outer tank (4), the liquid outlet (9) is located below the filter membrane (8), and the peristaltic pump (11) is used for feeding the filtrate flowing into the return tube (10) into the outer tank (4).
3. The cell microvesicle collection device according to claim 2, wherein the peristaltic pump (11) is clamped on the return tube (10).
4. The cell microvesicle collection device according to claim 2, wherein both ends of the return tube (10) are detachably connected to the return tube interface (12) and the drain port (9), respectively.
5. The cell microvesicle collecting device according to claim 1, wherein the upper end of the outer layer tank body (4) is provided with a mounting opening (13), the inner layer membrane bag (3) is fixedly connected in the mounting opening (13), and the upper end of the inner layer membrane bag (3) is provided with a liquid inlet (2).
6. The cell microvesicle collection device according to claim 5, wherein the upper end of the inner membrane bag (3) is further provided with an air hole (1).
7. Cell microvesicle collection device according to claim 1, characterized in that the side wall of the filtration device (7) is provided with a collection opening (14), the collection opening (14) being located at the upper end of the filter membrane (8).
8. The cell microvesicle collection device according to claim 7, wherein the filter (8) is disposed obliquely, and the collection port (14) is located at the upper end position of the lowest point of the filter (8).
9. Cell microvesicle collection device according to claim 1, wherein the filter device (7) is connected to a filter frame on its inner wall, on which the filter membrane (8) is mounted.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202322458895.0U CN220766993U (en) | 2023-09-11 | 2023-09-11 | Cell microvesicle collection device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202322458895.0U CN220766993U (en) | 2023-09-11 | 2023-09-11 | Cell microvesicle collection device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN220766993U true CN220766993U (en) | 2024-04-12 |
Family
ID=90604147
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202322458895.0U Active CN220766993U (en) | 2023-09-11 | 2023-09-11 | Cell microvesicle collection device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN220766993U (en) |
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2023
- 2023-09-11 CN CN202322458895.0U patent/CN220766993U/en active Active
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