CN218345470U - Cell culture bag fluid infusion device - Google Patents
Cell culture bag fluid infusion device Download PDFInfo
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- CN218345470U CN218345470U CN202221317848.3U CN202221317848U CN218345470U CN 218345470 U CN218345470 U CN 218345470U CN 202221317848 U CN202221317848 U CN 202221317848U CN 218345470 U CN218345470 U CN 218345470U
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- fluid infusion
- culture bag
- overflow ball
- funnel
- cell culture
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Abstract
The utility model belongs to the technical field of the medical instrument technique and specifically relates to just a cell culture bag fluid infusion device, including fluid infusion subassembly, culture bag and fluid infusion support, culture bag one end is equipped with the fluid infusion pipe, the fluid infusion subassembly includes funnel, a plurality of branch pipe, anti-overflow ball and connecting pipe, a plurality of branch pipe evenly connect in between funnel and the anti-overflow ball, connecting pipe one end and anti-overflow ball through connection, the connecting pipe other end is connected with fluid infusion pipe detachable, the fluid infusion support includes stores pylon, standing groove and counter weight box, the standing groove sets up in counter weight box back, the stores pylon sets up in the standing groove upside. Set up the fluid infusion subassembly, connect the fluid infusion subassembly on cultivateing the bag, add the culture solution in to the cultivation bag through the fluid infusion subassembly, make the fluid infusion process reduce the use of devices such as syringes, reduce the pollution risk, it is easy and simple to handle.
Description
Technical Field
The utility model belongs to the technical field of medical apparatus technique and specifically relates to a cell culture bag fluid infusion device.
Background
Cell culture (cellcell) refers to a method for simulating in vivo environment (sterility, proper temperature, pH value, certain nutritional conditions, etc.) in vitro to make it survive, grow, reproduce and maintain the main structure and function.
The fed-batch culture is that a certain amount of culture solution is firstly filled into a reactor, cells are inoculated under proper conditions for culture, the cells grow continuously, products are formed continuously, in the process, new nutrient components are continuously supplemented into a system along with the continuous consumption of nutrient substances, the cells are further metabolized for growth, and the products are taken out after the whole culture is finished. The culture bag is one of reactors commonly used by us, and in order to reduce the damage of air bubbles to cells during uniform mixing and sampling, the air in the culture bag is squeezed out after liquid supplement. However, the operation of the existing reactor is inconvenient during feeding, the liquid needs to be fed by an injector for multiple times, and the cell culture liquid is easy to splash and induce pollution when gas in a culture bag is extruded.
SUMMERY OF THE UTILITY MODEL
For solving above-mentioned culture bag fluid infusion operation inconvenience, cause the problem that the culture solution splashes easily during exhaust air, the utility model provides a cell culture bag fluid infusion device.
The utility model provides a technical scheme that its technical problem adopted is: the utility model provides a cell culture bag fluid infusion device, includes fluid infusion subassembly, cultivates bag and fluid infusion support, it is equipped with fluid infusion pipe to cultivate bag one end, the fluid infusion subassembly includes funnel, a plurality of branch pipe, anti-overflow ball and connecting pipe, a plurality of branch pipe evenly connect in between funnel and the anti-overflow ball, connecting pipe one end and anti-overflow ball through connection, the connecting pipe other end is connected with fluid infusion pipe detachable, the fluid infusion support includes stores pylon, standing groove and counter weight box, the standing groove sets up in counter weight box back, the stores pylon sets up in the standing groove upside.
As optimization, the funnel lower extreme be connected with the tee bend, the branch pipe is equipped with two, branch pipe one end and tee bend connection, the branch pipe other end is connected in anti-overflow ball upper portion, anti-overflow ball is inside to be cavity, the connecting pipe is connected in anti-overflow ball bottom, the connecting pipe sets up with the axle center with the funnel.
Preferably, the anti-overflow ball is hollow, and the inner diameter of the anti-overflow ball is larger than the diameter of the connecting pipe and the diameter of the branch pipe.
As an optimization, the placing groove is a groove body with an upward inclined opening, the bottom of the placing groove is flush with the bottom of the counterweight box, and the bottom of the placing groove is inclined.
Preferably, the inside of the counterweight box is provided with an accommodating space, the top of the counterweight box is provided with a sealing cover, one end of the sealing cover is rotatably connected with the top of the counterweight box, and a sealing rubber strip is arranged on the lower side of the sealing cover and the top of the counterweight box.
As optimization, the other end of the sealing cover is provided with a locking block, the outer side of the counterweight box is provided with a limiting hole, and the locking block is connected with the limiting hole in a matched mode.
Preferably, the hanging rack is provided with a hanging hole, the hanging hole penetrates through the hanging rack from top to bottom, a through hole is formed in one side of the hanging hole and one side of the hanging rack, the diameter of the hanging hole is smaller than the maximum diameter of the funnel, and the width of the through hole is larger than the minimum diameter of the funnel.
Preferably, the distance between the hanging rack and the top of the placing groove is larger than the distance between the lower end of the funnel and the culture bag.
The beneficial effect of this scheme is: a cell culture bag fluid infusion device has the following benefits:
set up the fluid infusion subassembly, connect the fluid infusion subassembly on cultivateing the bag, add the culture solution to cultivateing the bag through the fluid infusion subassembly, make the fluid infusion process reduce the use of devices such as syringes, the pollution abatement risk, easy and simple to handle, will cultivate the bag during fluid infusion and set up on the standing groove, the funnel is placed on the stores pylon, air gathering in cultivateing the bag is at cultivateing the bag top, conveniently extrudes, the design of anti-overflow ball can effectively reduce splashing of culture solution, reduce the waste of culture solution, reduce the pollution probability.
Drawings
Fig. 1 is a schematic axial view of the present invention.
Figure 2 is the utility model discloses fluid infusion subassembly axle side sketch map.
Fig. 3 is a schematic front view of the fluid infusion assembly of the present invention.
Figure 4 is the utility model discloses fluid infusion support shaft side schematic diagram.
Figure 5 is the utility model discloses fluid infusion support back axoplasm sketch map.
Fig. 6 is the schematic diagram of the bottom shaft side of the fluid infusion support of the utility model.
The anti-overflow device comprises a culture bag 1, a culture bag 2, a liquid supplementing pipe 3, a funnel 4, a branch pipe 5, an anti-overflow ball 6, a connecting pipe 7, a hanging frame 8, a placing groove 9, a counterweight box 10, a sealing cover 11, a sealing rubber strip 12, a locking block 13, a limiting hole 14, a hanging hole 15 and a through hole.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are some, but not all, embodiments of the present invention. The components of embodiments of the present invention, as generally described and illustrated in the figures herein, may be arranged and designed in a wide variety of different configurations. Thus, the following detailed description of the embodiments of the invention, as presented in the figures, is not intended to limit the scope of the invention, as claimed, but is merely representative of selected embodiments of the invention. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative efforts all belong to the protection scope of the present invention.
It should be noted that: like reference numbers and letters refer to like items in the following figures, and thus, once an item is defined in one figure, it need not be further defined and explained in subsequent figures.
In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", and the like indicate the position or positional relationship based on the position or positional relationship shown in the drawings, or the position or positional relationship which is usually placed when the product of the present invention is used, and are only for convenience of description and simplification of the description, but do not indicate or imply that the device or element referred to must have a specific position, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first," "second," "third," and the like are used solely to distinguish one from another and are not to be construed as indicating or implying relative importance.
As shown in fig. 1 and 4, a cell culture bag fluid infusion device, includes fluid infusion subassembly, culture bag 1 and fluid infusion support, culture bag 1 one end is equipped with fluid infusion pipe 2, the fluid infusion subassembly includes funnel 3, a plurality of branch pipe 4, anti-overflow ball 5 and connecting pipe 6, a plurality of branch pipe 4 evenly connect in between funnel 3 and the anti-overflow ball 5, 6 one end of connecting pipe and anti-overflow ball 5 through connections, the 6 other end of connecting pipe and the detachable of fluid infusion pipe are connected, the fluid infusion support includes stores pylon 7, standing groove 8 and counter weight box 9, standing groove 8 sets up in the 9 backs of counter weight box, stores pylon 7 sets up in 8 upsides of standing groove. The liquid supplementing pipe 2 of the culture bag 1 is provided with a checkpoint, and the culture bag 1 is also connected with a sampling port with a knob.
As shown in fig. 2, the lower end of the funnel 3 is connected with a tee joint, the branch pipes 4 are provided with two, one end of each branch pipe 4 is connected with the tee joint, the other end of each branch pipe 4 is connected to the upper part of the anti-overflow ball 5, the anti-overflow ball 5 is hollow, the connecting pipe 6 is connected to the bottom of the anti-overflow ball 5, and the connecting pipe 6 and the funnel 3 are coaxially arranged.
As shown in fig. 2, the anti-spill ball 5 is hollow, and the inner diameter of the anti-spill ball 5 is larger than the diameter of the connecting pipe 6 and the diameter of the branch pipe 4.
As shown in fig. 4, the placing groove 8 is a groove body with an opening inclined upwards, the bottom of the placing groove 8 is flush with the bottom of the counterweight box 9, and the bottom of the placing groove 8 is inclined.
As shown in fig. 5, an accommodating space is provided inside the counterweight box 9, a sealing cover 10 is provided on the top of the counterweight box 9, one end of the sealing cover 10 is rotatably connected with the top of the counterweight box 9, and a sealing rubber strip 11 is provided on the lower side of the sealing cover 10 and the top of the counterweight box 9.
As shown in fig. 5, a locking block 12 is arranged at the other end of the sealing cover 10, a limiting hole 13 is arranged on the outer side of the counterweight box 9, and the locking block 12 is connected with the limiting hole 13 in a matching manner.
As shown in fig. 3 and 4, a hanging hole 14 is formed in the hanging rack 7, the hanging hole 14 penetrates through the hanging rack 7 from top to bottom, a through hole 15 is formed in one side of the hanging hole 14 and one side of the hanging rack 7, the diameter of the hanging hole 14 is smaller than the maximum diameter of the funnel 3, and the width of the through hole 15 is larger than the minimum diameter of the funnel 3.
The distance between the hanging rack 7 and the top of the placing groove 8 is larger than the distance between the lower end of the funnel 3 and the culture bag 1.
When the device is used specifically, the disposable liquid supplementing assembly is taken out, and the funnel 3 is hung in the hanging hole 14 of the hanging frame 7;
the screw cap at the end part of the liquid supplementing pipe 2 of the culture bag 1 is opened, the liquid supplementing pipe 2 is connected with the connecting pipe 6, and the culture bag 1 is placed in the inclined placing groove 8;
measuring a fresh cell culture solution with a proper volume by using a pipettor, and adding the fresh cell culture solution into the funnel 3;
the culture solution enters the culture bag 1 through the funnel 3, the tee joint, the branch pipe 4, the anti-overflow ball 5, the connecting pipe 6 and the liquid supplementing pipe 2, the culture bag is extruded to enable gas in the culture bag 1 to be upwards discharged along the liquid supplementing pipe 2 and the liquid supplementing assembly, and the anti-overflow ball 5 can effectively prevent the liquid from splashing;
after the liquid supplementing is finished, the connecting pipe 6 is taken down from the liquid supplementing pipe 2, the screw cap on the liquid supplementing pipe 2 is screwed on, and the used liquid supplementing device is discarded.
The above embodiments are only specific cases of the present invention, and the protection scope of the present invention includes but is not limited to the product forms and styles of the above embodiments, any cell culture bag feeding device according to the claims of the present invention and any suitable changes or modifications made to it by those skilled in the art should fall within the protection scope of the present invention.
Claims (8)
1. The utility model provides a cell culture bag fluid infusion device which characterized in that: including fluid infusion subassembly, cultivation bag and fluid infusion support, cultivation bag one end is equipped with the fluid infusion pipe, the fluid infusion subassembly includes funnel, a plurality of branch pipe, anti-overflow ball and connecting pipe, a plurality of branch pipe evenly connect in between funnel and the anti-overflow ball, connecting pipe one end and anti-overflow ball through connection, the connecting pipe other end is connected with fluid infusion pipe detachable, the fluid infusion support includes stores pylon, standing groove and counter weight box, the standing groove sets up in counter weight box back, the stores pylon sets up in the standing groove upside.
2. The cell culture bag feeding device according to claim 1, wherein: the anti-overflow ball connecting device is characterized in that the lower end of the funnel is connected with a tee joint, the branch pipes are two, one end of each branch pipe is connected with the tee joint, the other end of each branch pipe is connected to the upper portion of the anti-overflow ball, the anti-overflow ball is hollow, the connecting pipe is connected to the bottom of the anti-overflow ball, and the connecting pipe and the funnel are coaxially arranged.
3. The cell culture bag feeding device according to claim 2, wherein: the anti-overflow ball is hollow, and the inner diameter of the anti-overflow ball is larger than the diameter of the connecting pipe and the diameter of the branch pipe.
4. The cell culture bag feeding device according to claim 1, wherein: the standing groove be the ascending cell body of opening slant, the standing groove bottom flushes the setting with counter weight box bottom, the setting of standing groove bottom surface slope.
5. The cell culture bag feeding device according to claim 4, wherein: the counterweight box is characterized in that an accommodating space is formed inside the counterweight box, a sealing cover is arranged at the top of the counterweight box, one end of the sealing cover is rotatably connected with the top of the counterweight box, and a sealing rubber strip is arranged on the lower side of the sealing cover and the top of the counterweight box.
6. The cell culture bag feeding device according to claim 5, wherein: the other end of the sealing cover is provided with a locking block, the outer side of the counterweight box is provided with a limiting hole, and the locking block is connected with the limiting hole in a matched mode.
7. The cell culture bag feeding device according to claim 6, wherein: the hanging rack is provided with a hanging hole, the hanging hole penetrates through the hanging rack from top to bottom, a through opening is formed in one side of the hanging hole and one side of the hanging rack, the diameter of the hanging hole is smaller than the maximum diameter of the funnel, and the width of the through opening is larger than the minimum diameter of the funnel.
8. The cell culture bag feeding device according to claim 7, wherein: the distance between the hanging rack and the top of the placing groove is larger than the distance between the lower end of the funnel and the culture bag.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202221317848.3U CN218345470U (en) | 2022-05-30 | 2022-05-30 | Cell culture bag fluid infusion device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202221317848.3U CN218345470U (en) | 2022-05-30 | 2022-05-30 | Cell culture bag fluid infusion device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN218345470U true CN218345470U (en) | 2023-01-20 |
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ID=84921872
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202221317848.3U Active CN218345470U (en) | 2022-05-30 | 2022-05-30 | Cell culture bag fluid infusion device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN218345470U (en) |
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2022
- 2022-05-30 CN CN202221317848.3U patent/CN218345470U/en active Active
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