CN216891011U - Electric magnetic frame for nucleic acid extraction - Google Patents
Electric magnetic frame for nucleic acid extraction Download PDFInfo
- Publication number
- CN216891011U CN216891011U CN202220564506.5U CN202220564506U CN216891011U CN 216891011 U CN216891011 U CN 216891011U CN 202220564506 U CN202220564506 U CN 202220564506U CN 216891011 U CN216891011 U CN 216891011U
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- CN
- China
- Prior art keywords
- nucleic acid
- iron core
- rack
- isolation according
- acid isolation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 108020004707 nucleic acids Proteins 0.000 title claims abstract description 31
- 102000039446 nucleic acids Human genes 0.000 title claims abstract description 31
- 150000007523 nucleic acids Chemical class 0.000 title claims abstract description 31
- 238000000605 extraction Methods 0.000 title claims abstract description 15
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical group [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 claims abstract description 42
- 238000009434 installation Methods 0.000 claims abstract description 24
- 238000002955 isolation Methods 0.000 claims description 12
- 229910000976 Electrical steel Inorganic materials 0.000 claims description 4
- 230000005611 electricity Effects 0.000 claims description 4
- 229910052742 iron Inorganic materials 0.000 claims description 4
- 239000000463 material Substances 0.000 claims description 4
- 239000011148 porous material Substances 0.000 claims description 2
- 239000011324 bead Substances 0.000 abstract description 22
- 239000002184 metal Substances 0.000 description 10
- 229910052751 metal Inorganic materials 0.000 description 10
- 238000000034 method Methods 0.000 description 8
- 239000007788 liquid Substances 0.000 description 5
- 230000005389 magnetism Effects 0.000 description 4
- 238000001179 sorption measurement Methods 0.000 description 4
- 108090000623 proteins and genes Proteins 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- UQSXHKLRYXJYBZ-UHFFFAOYSA-N Iron oxide Chemical compound [Fe]=O UQSXHKLRYXJYBZ-UHFFFAOYSA-N 0.000 description 2
- 239000013543 active substance Substances 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 238000000746 purification Methods 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000000975 bioactive effect Effects 0.000 description 1
- 239000003990 capacitor Substances 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000003759 clinical diagnosis Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000003480 eluent Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000009169 immunotherapy Methods 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000001821 nucleic acid purification Methods 0.000 description 1
- 239000002773 nucleotide Substances 0.000 description 1
- 125000003729 nucleotide group Chemical group 0.000 description 1
- 238000006116 polymerization reaction Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
Images
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model relates to an electric magnetic rack for nucleic acid extraction, which comprises a rack body, wherein the rack body comprises a handheld part which extends along the vertical direction and is used for being held by an operator, the rack body also comprises an installation part which is arranged at the lower end of the handheld part and extends along the transverse direction, at least three iron cores which are arranged along the transverse direction at intervals are installed on the installation part, the length of each iron core extends along the vertical direction, the periphery of each iron core is annularly provided with a corresponding inductance coil, a battery which is electrically connected with the corresponding inductance coil through a circuit is arranged in the rack body, and a button switch for controlling the corresponding inductance coil to be powered on and powered off is arranged on the handheld part. The utility model provides an electric magnetic rack for extracting nucleic acid, which can take and place magnetic beads in a plurality of containers at one time.
Description
Technical Field
The utility model relates to the technical field of molecular biology and cell biology purification, in particular to an electric magnetic frame for extracting nucleic acid.
Background
In a plurality of technical fields such as molecular biology research, clinical diagnosis, biochemical extraction, food detection, immunotherapy and the like, rapid and efficient purification of bioactive substances such as proteins, nucleic acids and the like and cell sorting play an important role.
In the case of nucleic acids, the nucleic acids are biologically active substances, which are the genetic material of life, formed by the polymerization of a plurality of nucleotides. At present, the mainstream of nucleic acid purification methods is divided into two types, one is an adsorption column method, and the other is a magnetic bead adsorption method. The magnetic bead separation technology is a simple and efficient method for purifying the current nucleic acid, the magnetic bead is made of iron oxide particles with active groups (active genes can specifically adsorb substances to be purified and separated) coated on the surfaces of the particles, and the magnetic bead is added into a sample mixture and adsorbs the nucleic acid.
At the moment, magnetism is added on the side wall of the container, the magnetic beads combined with the nucleic acid are gathered on the wall of the container, and then other impurity solutions can be removed by directly pouring or gun suction, so that repeated centrifugation is avoided, and compared with an adsorption column method, the method is simpler and easier to realize, and is widely applied.
At present, the magnetic bead adsorption method is divided into two types of manual extraction and automatic instrument extraction according to the implementation form of nucleic acid extraction, the automatic instrument extraction needs to purchase the automatic nucleic acid extraction instrument, and the instrument cost is tens of thousands yuan; manual extraction generally requires purchasing a magnetic frame, the operation process is relatively complex, liquid can be absorbed once each time, and the efficiency is low.
SUMMERY OF THE UTILITY MODEL
The utility model aims to provide an electric magnetic rack for nucleic acid extraction, which can take and place magnetic beads in a plurality of containers at one time.
In order to solve the above technical problems, the present invention provides an electromagnetic rack for nucleic acid extraction, comprising:
an electric magnetic frame for nucleic acid extraction is characterized in that: including the support body, the support body includes along the handheld portion that is used for operating personnel handheld of vertical extension, the support body is still including setting up in the installation department along horizontal extension of handheld portion lower extreme, install the at least three iron core of arranging along horizontal interval on the installation department, the length of each iron core extends along upper and lower direction, the periphery of each iron core all encircles and is equipped with the inductance coils that corresponds, be provided with in the support body through the circuit with correspond the battery that the inductance coils electricity is connected, be provided with the button switch that the control corresponds inductance coils and gets electric, cut off the power supply in the handheld portion.
The utility model has the beneficial effects that: during the use, the handheld portion of operating personnel, through control button switch, can make each inductance coils get electric, and the iron core that corresponds inductance coils inboard like this will have magnetism, stretches into respectively each iron core to correspond in having the container of magnetic bead, and every iron core can adsorb the magnetic bead of a container, and this just so can adsorb out together the magnetic bead of a plurality of containers, cuts off the power supply to inductance coils afterwards, and the iron core loses magnetism, just can realize the separation of iron core and magnetic bead.
Further, the iron core is made of soft iron or silicon steel material.
Further, the number of the iron cores is 6, 8 or 12.
Furthermore, a slide rheostat is arranged on the circuit in series, and the button switch is connected with a resistance adjusting switch of the slide rheostat.
Furthermore, one side of one end of the handheld part, which is far away from the installation part, is provided with a hook structure.
Further, the inductance coils are connected in parallel.
Further, the iron core and the corresponding inductance coil are installed on a module support, an installation hole for installing the corresponding module support is formed in the installation portion, and the module support is detachably connected with the installation portion.
Further, the module bracket and the mounting portion are detachably connected through a screw.
Further, the periphery of module support is provided with the anodal sheetmetal and the negative pole sheetmetal that are connected with corresponding inductance coils electricity, and the direction removes on the pore wall of mounting hole to be equipped with and is used for with corresponding anodal sheetmetal, the electrically conductive complex positive contact pilotage of negative pole sheetmetal contact and negative contact pilotage, positive contact pilotage and negative contact pilotage with circuit connection.
Further, a contact pin return spring is arranged between the hole wall of the mounting hole and the corresponding contact pin.
Drawings
The above and other objects, features and advantages of exemplary embodiments of the present disclosure will become readily apparent from the following detailed description read in conjunction with the accompanying drawings. Several embodiments of the present disclosure are illustrated by way of example, and not by way of limitation, in the figures of the accompanying drawings and in which like reference numerals refer to like or corresponding parts and in which:
FIG. 1 is a schematic view showing the structure of one embodiment of an electromagnetic rack for nucleic acid isolation according to the present invention;
FIG. 2 is an enlarged view taken at A in FIG. 1;
FIG. 3 is a schematic view of the module support of FIG. 1 in cooperation with the core and inductor coils;
FIG. 4 is a schematic circuit diagram of an electric magnetic rack for nucleic acid isolation according to the present invention;
FIG. 5 is a schematic view showing the structure of a container set which is provided in association with an electromotive force rack for nucleic acid extraction according to the present invention;
description of reference numerals: 1. a hook structure; 2. a push button switch; 3. a battery; 4. a hand-held portion; 5. a frame body; 6. a positive electrode contact pin; 7. a negative contact pin; 8. an inductor coil; 9. an inductance module; 10. an iron core; 11. an installation part; 12. a contact pin mounting groove; 13. a stylus return spring; 14. a contact pin spring seat; 15. a positive electrode metal sheet; 16. a module support; 17. a screw; 18. a negative electrode metal piece; 19. a slide rheostat; 20. a container holder; 21. a container; 22. an electrical circuit.
Detailed Description
In order to facilitate an understanding of the utility model, the utility model is described in more detail below with reference to the accompanying drawings and specific examples. Preferred embodiments of the present invention are shown in the drawings. This invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete.
It is to be noted that, unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terminology used in the description of the utility model herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the utility model.
Examples of an electromagnetic rack for nucleic acid isolation according to the present invention are shown in FIGS. 1 to 5: including support body 5, the support body includes along vertical extension be used for handheld portion 4 of operating personnel handheld, handheld portion 4 is shaft-like structure, and the support body is still including setting up in the installation department 11 of the horizontal extension in edge of handheld portion lower extreme, and installation department 11 also is shaft-like structure, and the installation department is arranged with handheld portion is perpendicular.
The electric magnetic frame further comprises eight inductance modules 9 arranged at intervals along the length direction of the installation part, each inductance module comprises a module support 16, an iron core 10 and an inductance coil 8 annularly arranged on the periphery of the upper end of the iron core are arranged on the module support 16, in the embodiment, the module support 16 is of a hollow shell-shaped structure, the iron core is fixed on an upper shell plate and a lower shell plate of the module support, and the inductance coil 8 is arranged in an inner cavity of the module support. The core 10 is made of a soft iron (or silicon steel) material. The soft iron (or silicon steel) material demagnetizes fast, and when the power supply is turned off, the iron core loses magnetism fast.
The handheld portion of the frame body is internally provided with a battery 3 which is electrically connected with each inductance coil through a circuit, each inductance coil is connected in parallel in the circuit, and the handheld portion is provided with a button switch 2 which controls each inductance coil to be powered on or powered off.
The periphery of the upper end of the module support is provided with a positive electrode metal sheet 15, and the lower end of the module support is provided with a negative electrode metal sheet 18.
The installation part is provided with an installation hole for installing the corresponding module bracket, and the module bracket is detachably connected with the installation part. In this embodiment, the lower end of the module bracket is provided with a connection turning edge, and a screw 17 for realizing detachable connection between the module bracket and the installation part is arranged between the connection turning edge and the installation part.
The hole wall of the mounting hole is provided with contact pin mounting grooves 12 which extend along the transverse direction and are respectively arranged corresponding to the metal sheets, the upper contact pin mounting groove is provided with a positive contact pin 6 which is in contact and electric connection with the positive metal sheet in a guiding and moving mode, and the lower contact pin mounting groove is provided with a negative contact pin which is in contact and electric connection with the negative metal sheet 18 in a guiding and moving mode. A positive contact pin and a negative contact pin are connected to the circuit 22.
A contact pin return spring 13 is arranged between the hole wall of the mounting hole and the corresponding contact pin, the contact pin return spring 13 is used for applying an acting force towards the iron core direction to the corresponding contact pin, one end of the contact pin return spring is propped against the corresponding contact pin, the other end of the contact pin return spring is propped against a contact pin spring seat 14, the contact pin spring seat 14 is fixed on the hole wall of the mounting hole, and in the embodiment, the contact pin spring seat is of an annular structure. One end of the contact pin extends out of the contact pin spring seat and then is connected with the battery through a flexible lead, and meanwhile, one end of the contact pin, which is far away from the metal sheet, is also provided with a stopping and turning edge which is used for being in stopping fit with the contact pin spring seat so as to limit the moving limit of the contact pin towards the direction of the iron core.
The module support is in rotation stopping fit with the mounting hole, after the module support is inserted into the mounting hole, the module support is connected with the mounting part through a screw, one end of the positive contact pin is pressed against the positive metal sheet, and one end of the negative contact pin 7 is pressed against the negative metal sheet, so that the inductance coil is connected in series in the circuit 22. The inductance coil 8 and the iron core 11 adopt a modular structure, and the corresponding number of inductance modules can be selected as required to be installed, so that different capacitor groups can be matched.
One side of the one end of installation department is kept away from to handheld portion is provided with couple structure 1, and the couple structure has a groove of hanging, can be convenient hang electronic magnetic frame on moving liquid support.
The circuit is provided with a slide rheostat 19 in series, and the button switch 2 is connected with a resistance adjusting switch of the slide rheostat 19.
The container group matched with the present invention has a structure as shown in fig. 5, and includes a container holder 20 and eight containers 21 arranged on the container holder at intervals in the left-right direction, and the containers in this embodiment may be test tubes.
When the device is used, a liquid-transferring gun with 8 channels is adopted to transfer sample liquid into each container, a specified amount of magnetic beads are placed into each container, after the sample liquid is mixed and purified, nucleic acid is adsorbed on the magnetic beads, an operator holds the holding part of the device by hand, inserts each iron core into the corresponding container respectively, turns on the resistance adjusting switch and pushes the resistance adjusting switch to a proper current gear, the iron core generates magnetic force, the iron core can suck the magnetic beads away, the magnetic beads are placed in cleaning liquid, then the magnetic beads are placed in eluent by using the iron core, target products are eluted, and finally the magnetic beads are removed to leave the target products.
That is to say, the electric magnetic rack can realize the taking and placing of magnetic beads in a plurality of containers, and can improve the working efficiency.
In other embodiments of the present invention, if magnetic beads with different magnetic specifications are added into a container, the surface active groups of the magnetic beads with different magnetic specifications are different, and therefore the active substances to be selectively adsorbed are also different, so that different magnetic forces can be generated by using different resistance levels, and by sorting the magnetic beads, two different substances can be purified by colleagues in one sample.
In other embodiments of the utility model: the iron core and the inductance coil do not need to adopt a modular structure, the iron core is directly fixed on the mounting part, and the inductance coil is also directly mounted on the mounting part.
In the above description of the present specification, the terms "fixed," "mounted," "connected," or "connected," and the like, are to be construed broadly unless otherwise expressly specified or limited. For example, with the term "coupled", it can be fixedly coupled, detachably coupled, or integrally formed; can be mechanically or electrically connected; they may be directly connected or indirectly connected through intervening media, or they may be connected internally or in any other suitable relationship. Therefore, unless the specification explicitly defines otherwise, those skilled in the art can understand the specific meaning of the above terms in the present invention according to specific situations.
From the above description of the present specification, those skilled in the art will also understand the terms used below, terms indicating orientation or positional relationship such as "upper", "lower", "front", "rear", "left", "right", "length", "width", "thickness", "vertical", "horizontal", "top", "bottom", "inner", "outer", "axial", "radial", "circumferential", "central", "longitudinal", "transverse", "clockwise" or "counterclockwise" and the like are based on the orientation or positional relationship shown in the drawings of the present specification, it is for the purpose of facilitating the explanation of the utility model and simplifying the description, and it is not intended to state or imply that the devices or elements involved must be in the particular orientation described, constructed and operated, therefore, the above terms of orientation or positional relationship should not be understood or interpreted as limiting the present invention.
In addition, the terms "first" or "second", etc. used in this specification are used to refer to numbers or ordinal numbers only for descriptive purposes and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one of the feature. In the description of the present specification, "a plurality" means at least two, for example, two, three or more, and the like, unless specifically defined otherwise.
Finally, it should be noted that: the above examples are only intended to illustrate the technical solution of the present invention, but not to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, it should be understood by those of ordinary skill in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some technical features may be equivalently replaced; and such modifications or substitutions do not depart from the spirit and scope of the corresponding technical solutions of the embodiments of the present invention.
Claims (10)
1. An electric magnetic frame for nucleic acid extraction is characterized in that: including the support body, the support body includes along the handheld portion that is used for operating personnel handheld of vertical extension, the support body is still including setting up in the installation department along horizontal extension of handheld portion lower extreme, install the at least three iron core of arranging along horizontal interval on the installation department, the length of each iron core extends along upper and lower direction, the periphery of each iron core all encircles and is equipped with the inductance coils that corresponds, be provided with in the support body through the circuit with correspond the battery that the inductance coils electricity is connected, be provided with the button switch that the control corresponds inductance coils and gets electric, cut off the power supply in the handheld portion.
2. The electromotive force rack for nucleic acid isolation according to claim 1, wherein: the iron core is made of soft iron or silicon steel material.
3. The electromotive force rack for nucleic acid isolation according to claim 1, wherein: the number of the iron cores is 6, 8 or 12.
4. The electromotive force rack for nucleic acid isolation according to claim 1, wherein: the circuit is provided with a slide rheostat in series, and the button switch is connected with a resistance adjusting switch of the slide rheostat.
5. The electromotive force rack for nucleic acid isolation according to claim 1, wherein: one side of the handheld part, which is far away from one end of the mounting part, is provided with a hook structure.
6. The electromotive force rack for nucleic acid isolation according to claim 1, wherein: the inductance coils are connected in parallel.
7. The electric magnetic rack for nucleic acid isolation according to any one of claims 1 to 6, characterized in that: the iron core and the corresponding inductance coil are installed on a module support, an installation hole for installing the corresponding module support is formed in the installation portion, and the module support is detachably connected with the installation portion.
8. The electromotive force rack for nucleic acid isolation according to claim 7, wherein: the module bracket is detachably connected with the mounting part through a screw.
9. The electromotive force rack for nucleic acid isolation according to claim 7, wherein: the periphery of module support is provided with the anodal sheetmetal and the negative pole sheetmetal that correspond the inductance coils electricity and be connected, and the direction is removed to be equipped with on the pore wall of mounting hole and is used for with corresponding anodal sheetmetal, the electrically conductive complex anodal contact pilotage of negative pole sheetmetal contact and negative pole contact pilotage, anodal contact pilotage and negative pole contact pilotage with circuit connection.
10. The electric magnetic rack for nucleic acid isolation according to claim 9, wherein: and a contact pin return spring is arranged between the hole wall of the mounting hole and the corresponding contact pin.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202220564506.5U CN216891011U (en) | 2022-03-16 | 2022-03-16 | Electric magnetic frame for nucleic acid extraction |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202220564506.5U CN216891011U (en) | 2022-03-16 | 2022-03-16 | Electric magnetic frame for nucleic acid extraction |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN216891011U true CN216891011U (en) | 2022-07-05 |
Family
ID=82190961
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202220564506.5U Expired - Fee Related CN216891011U (en) | 2022-03-16 | 2022-03-16 | Electric magnetic frame for nucleic acid extraction |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN216891011U (en) |
-
2022
- 2022-03-16 CN CN202220564506.5U patent/CN216891011U/en not_active Expired - Fee Related
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| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20220705 |
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| CF01 | Termination of patent right due to non-payment of annual fee |